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Effect of Vegetal Complex Raw Food on Plasma Glucose and Lipid Composition in High Fat Diet-induced Diabetic Rats (식물류를 이용한 생식용 천연복합식품이 고지방 식이에 의한 당뇨병 유발쥐의 혈청 glucose함량 및 지질구성에 미치는 영향)

  • Lee Eun;Lee Mi-Kyung;Kim Pan-Gu;Kim Soo-Kyung;Lim Sang-Cheul
    • Korean Journal of Plant Resources
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    • v.18 no.2
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    • pp.270-278
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    • 2005
  • This experiment was conducted to develop and design a product that is convenient and helpful to both patients and ordinary people who want to maintain their health. In this experimentation, the experimental rat have been given with high fat food to induce Diabetes. Organic brown rice was given as their main energy source and natural complex food, most of materials were freeze-dried, was given to observe its effects on their glucose amount in the plasma. The results are as follows. During 28 days of basal diets, as observing their weight variations, normal group gained weights, but the natural complex food(NCF) groups lost their weights. As comparing the amount of neutral fat levels in the plasma between the diabetes group who just had basal group and one who had NCF, group with NCF groups' level was much lower than the other group who just had basal food. As comparing total cholesterol levels in the plasma, with diabetes group with basal food, $50\%$ and $100\%$ NCF groups' levels were lowered than that group. Regarding HDL-cholesterol level, all NCE groups had around 30mg/dl and there were not much fluctuation among groups. As for LDL-cholesterol level, the $100\%$ NCF group had the lowest level and showed almost the same as the normal group. The amounts of the glucose in the plasma were lowered in all NCF groups. Among those NCE groups, $100\%$ NCF group had 120mg/dl to reach almost the same as the normal group.

Dormancy and Germination Characteristics of Alpine Modest Primrose (Primula modesta var. hannasanensis T.Yamaz.) Seeds (설앵초의 종자 휴면과 발아특성)

  • Cho, Ju Sung;Lee, Cheol Hee
    • Korean Journal of Plant Resources
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    • v.30 no.4
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    • pp.372-377
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    • 2017
  • This research was performed to develop mass propagation method of Alpine modest primrose (Primula modesta var. hannasanensis T.Yamaz.) for improving the conservation and utilization of the species. Seeds were collected on August 2011 in Jeju-city and well-selected seeds were dry-stored at $4{\pm}1.0^{\circ}C$. Seed size ranged $0.44{\pm}0.07{\times}0.61{\pm}0.04mm$, and weight of 1,000 seeds was $51.78{\pm}0.021mg$. Thus the seed was classified as 'dwarf seeds'. As the result of dormancy characteristics, moisture content of freshly matured seeds increased rapidly by water-soaking treatment and seeds did not germinate at $20^{\circ}C$ for 4 weeks under the light condition. Therefore, seeds were estimated to have physiological dormancy. Germination conditions of dry-stored seeds were found to be $20^{\circ}C$ and light condition, and seeds did not germinated under dark condition regardless of all temperature regimes. Therefore, Alpine modest primrose seed was considered as photoblastic type. Percent germination (PG) and germination energy (GE) were greatly improved by soaking the seeds in $GA_3$ and kinetin for 24 hours. Especially, 200~500 mg/L $GA_3$ treatment resulted in the highest PG (95.5%) and GE (98.3%). So, chemical treatment such as $GA_3$ was thought to be a useful method for raising seedling uniformly.

Hepatoprotective Effect of Flavonol Glycosides Rich Fraction from Egyptian Vicia calcarata Desf. Against $CCl_4$-Induced Liver Damage in Rats

  • Singab, Abdel Nasser B.;Youssef, Diaa T.A.;Noaman, Eman;Kotb, Saeed
    • Archives of Pharmacal Research
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    • v.28 no.7
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    • pp.791-798
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    • 2005
  • The hepatoprotective activity of flavonol glycosides rich fraction (F-2), prepared from 70% alcohol extract of the aerial parts of V calcarata Desf., was evaluated in a rat model with a liver injury induced by daily oral administration of $CCl_4$ (100 mg/kg, b.w) for four weeks. Treatment of the animals with F-2 using a dose of (25 mg/kg, b.w) during the induction of hepatic damage by $CCl_4$ significantly reduced the indices of liver injuries. The hepatoprotective effects of F-2 significantly reduced the elevated levels of the following serum enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH). The antioxidant activity of F-2 markedly ameliorated the antioxidant parameters including glutathione (GSH) content, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), plasma catalase (CAT) and packed erythrocytes glucose-6-phosphate dehydrogenase (G6PDH) to be comparable with normal control levels. In addition, it normalized liver malondialdehyde (MDA) levels and creatinine concentration. Chromatographic purification of F-2 resulted in the isolation of two flavonol glycosides that rarely occur in the plant kingdom, identified as quercetin-3,5-di-O-$\beta$-D-diglucoside (5) and kaempferol-3,5-di-O-$\beta$-D-diglucoside (4) in addition to the three known compounds identified as quercetin-3-O-$\alpha$-L-rhamnosyl- (${\rightarrow}6$)-$\beta$-D-glucoside [rutin, 3], quercetin-3-O-$\beta$-D-glucoside [isoquercitrin, 2] and kaempferol-3-O-$\beta$-D-glucoside [astragalin, 1]. These compounds were identified based on interpretation of their physical, chemical, and spectral data. Moreover, the spectrophotometric estimation of the flavonoids content revealed that the aerial parts of the plant contain an appreciable amount of flavonoids (0.89%) calculated as rutin. The data obtained from this study revealed that the flavonol glycosides of F-2 protect the rat liver from hepatic damage induced by $CCl_4$ through inhibition of lipid peroxidation caused by $CCl_4$ reactive free radicals.

Cloning of Cytochrome P450 Gene involved in the Pathway of Capsidiol Biosynthesis in Red Pepper Cells (고추세포에서 Capsidiol 생합성을 유도하는 Cytochrome P450 유전자의 탐색)

  • Kwon, Soon-Tae;Kim, Jae-Sung;Jung, Do-Cheul;Jeong, Jeong-Hag;Hwang, Jae-Moon;Oh, Sei-Myoung
    • Journal of Life Science
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    • v.13 no.6
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    • pp.879-888
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    • 2003
  • In order to measure the enzyme activity of 5-epi-aristolochene hydroxylase, one of cytochrome P450 (P450) enzymes in eicitor-treated pepper cell, we used in vivo assay method and demonstrated a dramatic suppression of the activity by P450-inhibitors, ancymidol and ketocornazole. Using RT-PCR method with degenerate primer of the well conserved domains found within most P450-enzymes, and using cDNA library screening method, one distinct cDNA, being designated P450Hy01, was successfully isolated from elicitor-treated pepper cells. P450Hy01 mRNA was all induced in elicitor-treated cells whereas never induced in control cells. Moreover, levels of P450Hy01 expression were highly correlated with the levels of extracellular capsidiol production by different elicitors in cell cultures. P450Hy01 transcript was also induced by several other elicitors such as, cellulase, arachidonic acid, jasmonic acid, yeast extract as well as UV stress. P450Hy01 sequence contained high probability amino acid matches to known Plant P450 genes and ORF with a conserved FxxGxRxCxG heme-binding domain. P450Hy01 cDNA showed 98% of homology in sequence of nucleotide as well as amino acid to 5-epi-aristolochene-1, 3-hydroxylase (5EAl, 3H) which has been isolated in tobacco cells, suggesting that P450Hy01 is prominent candidate gene for P450-enzyme encoding 5EAl, 3H in pepper cell.

A Study on the Changes in Heavy Metal Emissions when Using Mixed Fuel in a Thermal Power Plant (화력발전소의 혼합연료 사용에 따른 중금속 배출량 변화 연구)

  • Song, Youngho;Kim, Ok;Park, Sanghyun;Lee, Jinheon
    • Journal of Environmental Health Sciences
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    • v.44 no.1
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    • pp.63-75
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    • 2018
  • Objectives: The aim of this research is to explore the total heavy metals from a coal-fired power plant burning bituminous coal with wood pellets due to the implementation of the Renewable Portfolio Standard policy (RPS, 10% of electricity from renewable energy resources by 2023). Methods: The research was carried out by collecting archival data and using the USEPA's AP-42 & EMEP/EEA compilation of emission factors for use in calculating emissions. The Monte Carlo method was also applied for carrying out the calculations of measurement uncertainty. Results: In this paper, the results are listed as follows. Sb was measured at 110 kg (2015) and calculated as 165 kg (2019) and 201 kg (2023). Cr was measured at 1,597 kg (2015) and calculated as 1,687 kg (2019) and 1,728 kg (2023). Cu was measured at 2,888 kg (2015) and calculated as 3,133 kg (2019) and 3,264 kg (2023). Pb was measured at 2,580 kg (2015) and calculated as 2,831 kg (2019) and 2,969 kg (2023). Mn was measured at 3,011 kg (2015) and calculated as 15,034 kg (2019) and 23,014 kg (2023). Hg was measured at 510 kg (2015) and calculated as 513 kg (2019) and 537 kg (2023). Ni was measured at 1,720 kg (2015) and calculated as 1,895 kg (2019) and 1,991 kg (2023). Zn was measured at 7,054 kg (2015) and calculated as 9,938 kg (2019) and 11,778 kg (2023). Se was measured at 7,988 kg (2015) and calculated as 7,663 kg (2019) and 7,351 kg (2023). Conclusion: This shows that most heavy metals would increase steadily from 2015 to 2023. However, Se would decrease by 7.9%. This analysis was conducted with EMEP/EEA's emission factors due to the limited emission factors in South Korea. Co-firewood pellets in coal-fired power plants cause the emission of heavy metals. For this reason, emission factors at air pollution control facilities would be presented and the replacement of wood pellets would be needed.

Modeling of Medium Temperature Drops of the Elevated-bench Hydroponics for Strawberry Cultivation during Low Temperature Season (저온기 딸기 고설 수경재배시 온실기온에 따른 배지내 온도강하 모델 개발)

  • Park, Jae-Wan;Ha, Yu-Shin;Kim, Ki-Dong;Park, Dae-Heum;Lee, Ki-Myung;Jun, Ha-Joon;Kwon, Soon-Gu;Choi, Won-Sik;Chung, Sung-Won
    • Journal of Bio-Environment Control
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    • v.19 no.3
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    • pp.123-129
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    • 2010
  • A study on modeling of medium temperature drops of the elevated-bench hydroponic system for strawberry cultivation during low temperature season was conducted. Four different conditions were used for the experiment. These consisted of two kinds of bed types (plant, V), four kinds of medium (rice, perlite, rice hulls80% and peatmoss20%, perlite80% and peatmoss20%), two kinds of mulched bed (mulched, non mulched) and four kinds of greenhouse air temperature (l.5, 3.2, 5.0, $6.7^{\circ}C$), and the results were summarized as follows: Temperature drop of medium in the V-bed was slower than that in the plant bed, showing better insulation effect of V-bed. Temperature drop of medium with mulching on the top of the bed was slower than the case without mulching, as a result, the beneficial effect of temperature drop was appeared in mulched bed. Linear regression of the temperature descent rate and the temperature difference between medium and air showed significant correlation. The regression equation for the Pearlite80% and Peatmoss20% in the V-bed was f(x) = -0.2656 + 0.1345x at the $R^2$ of 0.9269. Using the model, the temperature drop during night can be predicted for the various media at the different depths.

Gamma Radiation Sensitivity and Quantitative Characters in M1 Generation of Heteropappus hispidus (Thunb.) (갯쑥부쟁이(Heteropappus hispidus (Thunb.))의 감마방사선 감수성과 M1 세대 형질변동 특징)

  • Oh, Byung-Kwon;Hong, Kyung-Ae;Song, Sung-Jun;Lee, Sun-Joo;Lee, Young-Il;U., Zang-Kual
    • Korean Journal of Environmental Agriculture
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    • v.22 no.4
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    • pp.294-299
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    • 2003
  • The seeds of naturally growing Heteropappus hispidus (Thunb.) were treated by nine different doses (0, 10, 20, 40, 80, 120, 160, 200, 300, 400 Gy) of gamma rays to investigate their germination rate and to quantity the characteristics of their germinated plants as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120Gy was not greatly affected but was rapidly decreased at over 160Gy. It seemed that lethal dose ($LD_{50}$) of germination was 160Gy. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40Gy. Multi-shoots were developed over 80Gy. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the high dose of gamma rays could be utilized as the morphological markers for the mutant selection of Heteropappus hispidus (Thunb.).

Mechanical Properties of Concrete Using Recycled Coarse Aggregate from Nuclear Power Plant Simulated Concrete (원자력발전소 모의 콘크리트로부터 생산된 순환 굵은 골재 활용 콘크리트 역학적 특성)

  • Lee, Seong-Cheol;Shin, Kyung-Joon;Kim, Chang-Lak
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.8 no.2
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    • pp.167-174
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    • 2020
  • Many researches have been conducted to utilize recycled aggregates in Korea, but since most sources of recycled aggregates are not clear, there is a lot of uncertainty in applying the existing research results on recycle of aggregates generated from nuclear power plants. In this study, therefore, in order to investigate the possibility of recycling coarse aggregates generated through dismantling of nuclear power plants in Korea, recycled coarse aggregates were produced from concrete simulating nuclear power plants in Korea. Using the recycled coarse aggregates, concrete was mixed in consideration of the mixing ratio of the recycled coarse aggregates, and the mechanical properties were experimentally investigated. From the test results, as the mixing ratio of recycled coarse aggregates increased. concrete compressive strength, tensile strength, and elastic modulus generally decreased up to 36, 37, and 27% from the mechanical properties of normal concrete, respectively. Therefore, it can be concluded that limitation on the mixing ratio of recycled coarse aggregates is necessary when coarse aggregates are recycled through dismantling of nuclear power plants.

Uranium Levels in Soil and Plant, and Estimation of Its Intake by the Residents at the Uranium Deposited Area (우라늄광 부근에서 우라늄의 토양 및 식물체중 함량과 주민들에 의한 체내 집적량추정)

  • U, Zang-Kual;Song, Ki-Joon;Kim, Tai-Soon
    • Applied Biological Chemistry
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    • v.22 no.4
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    • pp.221-226
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    • 1979
  • The soils and plants were sampled from 26 sites of Deogpyeongri, Goisangun, which had been found to be one of the uranium deposit areas. Uranium levels of the samples were determined and the amount of uranium intake by the residents through the food-chains was estimated. The average uranium concentration of Deogpyeongri soils was 15.5ppm with a range of from 4.9 to 43.6ppm showing rather higher values than those of control area, Yangjugun and Icheongun, Gyeonggi-do. The average uranium content of the plant samples from Deogpyeongri was 0.69ppm, about twice the uranium concentration of the control samples. The daily intake of uranium by an adult lived on the agricultural food stuffs produced in Deogpyeongri, was estimated to be about $247{\mu}g$, eqivalent to $0.83{\times}10^{-4}{\mu}Ci$, which is much higher activity compared to the daily intake of uranium by New York citizen, $1.3{\mu}g$. However the calculated uranium level accumulated in the human body of Deogpyeong area was $2.03{\times}10^{-4}{\mu}Ci$ which is still lower than $0.2{\mu}Ci$, the maximum permissible burden in total body recommended by the ICRP.

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Determination of the Period of the Formation and Size of Sieve Element Area and Sieve Pore (Streptanthus tortus 조직배양 세포에서 사공의 형성시기와 사공 영역과 사공의 크기 결정)

  • Cho, Bong-Heuy
    • Journal of Plant Biotechnology
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    • v.29 no.1
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    • pp.41-44
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    • 2002
  • During the phloem development from parenchyma cells in a suspension culture of Streptanthus induced sucrose carrier and glucose carrier disappeared. Sieve element area and sieve pore induced suspension culture of Streptanthus were formed almost at the last period of the synthesis of sieve endoplasmic reticulum (SER) and p-protein. The new synthesized cell wall begann to digeste only after the new cell wall was surrounded by SER. The digested region of the cell wall and the formed region of sieve pore were regular comparatively. The completed sieve pore was an oval form, and the outer portion of sieve pore varied, ca 1.2 ${\mu}{\textrm}{m}$~1.6 ${\mu}{\textrm}{m}$ in longitudinal, 0.8 ${\mu}{\textrm}{m}$~1.3 ${\mu}{\textrm}{m}$ in tangential, and the inner size of sieve pore was irregular form of a star-like shape. The number of sieve pore between sieve cells was ca 2~7 per ${\mu}{\textrm}{m}$$^2$ and the sieve pore wall with callose was 0.05 ${\mu}{\textrm}{m}$~0.07 ${\mu}{\textrm}{m}$ in thickness. The energy for the formation of sieve element area and sieve pore might be supplied by mitochondria near the new cell wall and the role of SER remains to be illucidated.