• Asian-Australasian Journal of Animal Sciences
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• 제13권7호
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• pp.1026-1034
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• 2000

As described here, most recently developed methods for improving reproduction performance of domesticated animals such as cattle, swine and chicken have been considered to be also usable for restoring some sorts of endangered and/or extinct wild animals in the very near future. Especially, the techniques for in vitro storage of gametes obtained from dead animals shortly after the death, probably 24 h following the sacrifice are also available for obtaining some of experimental specimens. In case of the endangered animals, nobody will be allowed to use any tissues from the living animals, therefore, e.g., the use of skin tissues from these bodies is another possibility of restoring the living animals. Regarding the use of skin tissues, the most highly usable tools must be the cloning techniques for reviving rare cells from the living body. Most possible techniques for cloning cells is nuclear transfer from rare species to highly relative species, and this is the case of germ cells, e.g., primordial germ cells (PGCs) of avian species. One of the possibilities is the nuclear transfer of Crested Ibis (Nipponia nippon) to the PGCs of chicken, resulting in the PGCs with transferred nucleus from the ibis. In mammalian species, the same procedure as in the case of birds would be successful, e.g., the removed nucleus from Giant Pandas will be transferred to the cell, such as somatic cells or germ cells from black bears or lesser pandas, leading to the production of transnucleared cells in the body of female black bears. These two cases are most promising techniques for reviving endangered animals in the world, particularly in Asian countries, mainly in China. As a conclusion, possible production of cloned animals carrying transnucleared cells from endangered animals, such as Giant Pandas and Crested Ibis, may be reproduced gradually in the near future. Scientists are, therefore, required to convert the paradigm from domestic animals to wild animals, including endangered and/or extinct animals on the earth.

• Journal of Ecology and Environment
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• 제47권4호
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• pp.200-210
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• 2023

Background: Cambaroides similis is an endangered candidate species living in the stream of South Korea. Freshwater crayfish is known to decline rapidly not only domestically, but also internationally. Its decline is projected to be further exacerbated due to climate change. Understanding physical characteristics of the habitat is crucial for the conservation of an organism. However, comprehensive data regarding the distribution and physical habitat characteristics of C. similis are currently unavailable in South Korea. Thus, the objective of this study was to ascertain preferred ranges for water depth, current velocity, and streambed substrate of C. similis using Weibull model. Results: In this study, C. similis was found at 59 sites across 12 regions in South Korea. Its optimal water depth preferences ranged from 11.9 cm to 30.1 cm. Its current velocity preferences ranged from 9.8 cm s^-1 to 29.1 cm s^-1. Its substrate preferences ranged from -5.1 𝜱_m to -2.5 𝜱_m. Median values of central tendency were determined as follows: water depth of 21.4 cm, current velocity of 21.2 cm s^-1, and substrate of -4.1 𝜱_m. Mean values of central tendency were determined as follows: water depth of 21.8 cm, current velocity of 22.0 cm s^-1, and substrate of -4.4 𝜱_m. Mode values of central tendency were determined as follows: water depth of 21.7 cm, current velocity of 20.1 cm s^-1, and substrate of -3.7 𝜱_m. Conclusions: Based on habitat suitability analysis, physical microhabitat characteristics of C. similis within a stream were identified as Run section with coarse particle substrate, low water depth, and slow current velocity. Due to high sensitivity of these habitats to environmental changes, prioritized selection and assessment of threats should be carried out as a primary step.

• Bulletin of the Korean Chemical Society
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• 제32권8호
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• pp.2732-2736
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• 2011

In pH 4.2 HAc-NaAc buffer solution, horseradish peroxidase (HRP) catalyzed $H_2O_2$ oxidation of nanosilver to form $Ag^+$. After centrifugation, $Ag^+$ in the supernatant can be measured by graphite furnace atomic absorption spectrophotometry (GFAAS) at the silver absorption wavelength of 328.1 nm. When HRP concentration increased, the $Ag^+$ concentration in the supernatant increased, and the absorption value enhanced. The HRP concentration in the range of 0.84-50 $ng{\cdot}mL^{-1}$ was linear to the enhanced absorption value (${\Delta}A$), with a regression equation of ${\Delta}A$=0.012C+0.11, correlation coefficient of 0.9988, and detection limit of 0.41 $ng{\cdot}mL^{-1}$ HRP. The proposed GFAAS method was used to detect HRP in waste water samples, with satisfactory results.

• Journal of Species Research
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• 제9권1호
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• pp.35-45
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• 2020

In 2016 and 2017, as part of a comprehensive investigation to identify the prokaryotic species in Korea, a total of 12 bacterial strains were isolated from the gastrointestinal tract and/or fecal samples of four endangered species, including reptile, bird, and marine and terrestrial mammals. Phylogenetic analysis with the 16S rRNA gene sequence was used to assign these strains to the phyla, Firmicutes, Actinobacteria or Proteobacteria. Furthermore, most of the strains Firmicutes belonged to the order Lactobacillales. Interestingly, 12 of the isolated strains have not been previously reported from the Korean Peninsula. Also, based on their high 16S rRNA gene sequence similarities(>98.7%) and formation of strong monophyletic clades with the closest type species, each isolated strain of isolates was assigned to an independent, predefined bacterial species. Gram-stain reaction, colony and cell morphology, biochemical characteristics, isolation source, and NIBR IDs are described in the species description section.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.23-23
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• 2019

전남지역은 도서지역이 많고 지역적 특성으로 인하여 다양한 식물들이 자생하고 있는데 이중 희귀특산식물은 217종으로 조사되었다. 그 중에서 난대림 지역에 생육하고 있는 목본 희귀식물 및 특산식물을 중심으로 지리적 분포위치와 생육상태 등을 보고하고자 한다. 전남지역에 분포하는 멸종위기종(CR)은 35종으로 나타났으며 이 중 목본식물은 초령목(신안), 섬다래(완도), 조도만두나무(진도), 덩굴옻나무(여수), 거문도닥나무(고흥, 여수)등이 비교적 양호하게 자생하고 있다. 위기종(EN)은 37종에서 목본식물은 왕자귀(목포, 무안), 백서향(신안), 나도은조롱(완도), 박달목서(여수, 신안) 등이 자생하고 있다. 취약종(VU)은 54종으로 목본식물은 백량금(완도, 신안), 황근(완도), 붓순나무(진도), 모감주나무(완도), 산닥나무(영암, 진도)등이 자생하고 있다. 약관심종(LC)은 36종으로 목본식물은 검팽나무(여수), 이팝나무(완도, 고흥), 녹나무(완도, 진도), 털조장나무(보성) 등이 양호하게 자생하고 있다.

• 생태와환경
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• 제53권1호
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• pp.63-72
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• 2020

본 연구에서는 멸종위기종이 서식하는 4개 하천(금강, 지천, 황지천, 섬진강)에서 환경유전자(environmental DNA, eDNA)와 보편적 어구를 이용한 조사 방법을 적용하여 지점별 종 다양성을 확인하고, 이를 통해 eDNA의 활용을 고찰하였다. eDNA 조사를 통해서 확인된 종 수는 지점 평균(±표준편차) 19종(±4.4)이며, 이는 어구를 이용한 정량 조사의 10종(±4.8)과 비교하여 높게 나타났다. 대부분의 지점에서 eDNA 조사가 어구를 이용한 조사보다 효율이 높게 나타났다. 반면 eDNA 조사 결과 고유종 및 근연종에 대해서 동정의 오류가 확인되어, universal primer (MiFish primer set)에 대한 국내 적용의 한계를 확인하였다. 또한 멸종위기종의 서식 여부도 일부 종에 대해서 eDNA 조사 결과가 현장 조사 및 문헌과의 차이를 보였다. 현재 개발된 universal primer는 국내에서 서식하는 모든 담수종의 서식을 확인하는 데 있어서 결과의 신뢰성을 담보할 수 없기 때문에 universal primer의 보완 및 개발이 필요하며, 멸종위기종과 같은 특정종의 서식 확인을 위해서는 종특이적 마커 개발을 통한 적용이 고려되어야 한다. 마지막으로 eDNA의 현장 조사 방법에 대한 매뉴얼이 개발될 경우, 수생태계 조사에 대한 활용성이 증대될 수 있을 것이다.

• 환경영향평가
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• 제32권2호
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• pp.83-93
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• 2023

본 연구는 남생이의 출현에 영향을 주는 환경변수를 파악하고 남생이와 환경변수 간의 관계를 파악하기 위해 전라남도 구례군의 주요 저수지에서 남생이 생태 특성을 고려한 17개의 환경변수를 선정하여 서식지 환경 조사를 실시하고, 서식지와 비서식지의 조사 결과를 통계 분석하였다. 남생이의 서식지 적합성 모델을 개발한 결과, 변수 예측값은 logit (p) = -3.68 + (0.17 × 낙엽층두께) + (1.55 × 육상아교목층 피도량) + (0.71 × 육상초본층 피도량) + (0.96 × 수상초본층 피도량)으로 산출되었다. 남생이는 낙엽층두께가 두꺼울수록, 육상교목층 피도량, 육상관목층 피도량, 수상초본층 피도량이 높을수록 출현확률이 높아지는 것으로 파악되었다. 남생이의 출현에 영향을 주는 이러한 환경요인에 대한 정보는 추후 남생이 분포조사 시 도움이 되며, 남생이의 실질적인 서식지 보전을 위해 필요할 것으로 판단된다.

• 해양환경안전학회지
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• 제24권1호
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• pp.112-118
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• 2018

본 연구는 생물 다양성에 대한 중요성과 보호라는 관점에서 중국의 멸종위기종에 대한 법률과 관련된 제도를 분석하고 멸종위기 야생동물의 효율적인 보호를 위해 중국의 현행 야생동물 보호 법률에 대하여 요구되는 몇가지 개선점을 제시하는 것을 목적으로 수행되었다. <중화인민공화국 야생동물 보호법>이 1988년에 공포되고 시행된 이래 관리소홀로 인하여 시급한 조치가 필요하다는 사실을 알았다. 중국의 야생동물 보호법은 단조롭고 체계적이지 못해, 전근대적으로 현 시대의 수요에 부합하지 않기에 수정, 보완이 필요하다. 다른 국가들과 비교하였을 때, 입법취지와 감독체제, 보호범위 및 공중 참여제도, 입법 보호 체계의 혼란, 행정 보상제도의 미비와 실행의 난항, 법률 효율 저하, 내용의 공허성과 운용성의 부족, 생물 종 보호 명부가 법률 체계에 포함되어 있지 않는 등 많은 문제점을 가지고 있음을 확인할 수 있다. 이러한 일련의 문제점을 중국의 현 실정에 적합한 법률체계로 확립하기 위해 멸종위기종 보호 법률 법규의 체계상 혼란을 해결할 수 있는 개선방향에 대하여 제시한다.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.201-207
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• 2003

Tissue culture techniques arguably are an important approach for ex situ conservation of rare and endangered plant species. However, there is utmost importance on maintaining the genetic integrity of the introduced plants especially in tree species. To examine the genetic integrity of the micropropagated plants, we randomly screened few hardened plants of Syzygium travancoricum, a critically endangered tree taxon, using Randomly Amplified Polymorphic DNA (RAPD) markers. Twenty-three random. primers were tried and twenty-five polymorphic loci were identified. The dendrogram based on the Unweighted Pair-Group Method Arithmetic Average and Nei's similarity index depicted about 97% homology between the mother plants and micropropagated plants. Further, an attempt was made to reintroduce the micropropagated plants in the wild. Over three hundred small trees could be successfully established.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.32-32
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• 2019

The aim of this study was to provide basic guidelines for conservation and management of endangered plants in the national parks of Korea. Iris dichotoma Pall. (Iridaceae), which is a popular garden plant, is considered a second-class endangered species by Korean government and it is listed as a EN (Endangered) species in Red Data Book of Korea. We analyzed ecological conditions of I. dichotoma habitats based on vegetation properties and soil characteristics. This species which is known to inhabit in grassland adjacent to the ocean of lowlands slope and its population was located at an elevation of 8 m to 11 m. In the study sites, the mean of soil organic matter, total nitrogen and soil pH were 6.16%, 0.234% and 5.39 respectively. Additionally, the genetic variation and structure of three populations were assessed using ISSR (Inter Simple Sequence Repeat) markers. The genetic diversity of I. dichotoma (P = 59.46%, H = 0.206, S = 0.310) at the species level was relatively high. Analysis of molecular variance (AMOVA) showed 82.1% of the total genetic diversity was occurred in within populations and 17.9% variation among populations. Lastly, we developed predicted distribution model based on climate and topographic factors by applying SDMs (Species Distribution Models). Consequently, current status of I. dichotoma habitats is limited with natural factors such as the increase of the coverage rate of the herbs due to ecological succession. Therefore, it is essential to establish in situ and ex situ conservation strategies for protecting natural habitats and to require exploring potential and alternative habitats for reintroduction.