Bulletin of the Korean Chemical Society

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Graphite Furnace Atomic Absorption Spectrophotometric Determination of Trace Horseradish Peroxidase Using Nanosilver

  • Jiang, Zhi-Liang (Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, the Ministry of Education, School of Environment and Resource, Guangxi Normal University) ;
  • Tang, Ya-Fang (Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, the Ministry of Education, School of Environment and Resource, Guangxi Normal University) ;
  • Wei, Lin (Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, the Ministry of Education, School of Environment and Resource, Guangxi Normal University) ;
  • Liang, Ai-Hui (Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, the Ministry of Education, School of Environment and Resource, Guangxi Normal University)
  • Received : 2011.04.07
  • Accepted : 2011.07.04
  • Published : 2011.08.20
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Abstract

In pH 4.2 HAc-NaAc buffer solution, horseradish peroxidase (HRP) catalyzed $H_2O_2$ oxidation of nanosilver to form $Ag^+$. After centrifugation, $Ag^+$ in the supernatant can be measured by graphite furnace atomic absorption spectrophotometry (GFAAS) at the silver absorption wavelength of 328.1 nm. When HRP concentration increased, the $Ag^+$ concentration in the supernatant increased, and the absorption value enhanced. The HRP concentration in the range of 0.84-50 $ng{\cdot}mL^{-1}$ was linear to the enhanced absorption value (${\Delta}A$), with a regression equation of ${\Delta}A$=0.012C+0.11, correlation coefficient of 0.9988, and detection limit of 0.41 $ng{\cdot}mL^{-1}$ HRP. The proposed GFAAS method was used to detect HRP in waste water samples, with satisfactory results.

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