• Journal of Environmental Science International
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• v.26 no.6
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• pp.779-788
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• 2017

In this study, we analyzed the changes in the echolocation and prey-capture behavior of the horseshoe bat Rhinolophus ferrumequinum from search phase to capture time. The experiment was conducted in an indoor free-flight room fitted with an ultra-high-speed camera. We found that the bats searched for food while hanging from a structure, and capturing was carried out using the flight membrane. In addition, it was confirmed that the mouth and uropatagium were continuously used in tandem during the capturing process. Furthermore, using Constant Frequency (CF), we confirmed that the prey catching method reflected the wing morphology and echolocation pattern of R. ferrumequinum. The echolocation analysis revealed that the pulse duration, pulse interval, peak frequency, start-FM-bandwidth, and CF duration decreased as the search phase approached the terminal phase. Detailed analysis of echolocation pulse showed that the end-FM bandwidth, which increases as it gets nearer to the capture time of prey, was closely related to the accurate grasp of the location of an insect. At the final moment of prey capture, the passive listening that stopped the divergence of the echolocation was identified; this was determined to be the process of minimizing the interruption from the echo of the echolocation call emitted from the bat itself and sound waves emitted from the prey.

• BMB Reports
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• v.38 no.3
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• pp.294-299
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• 2005

In this report, we describe the high-yield secretory expression of the recombinant human anti-HBsAg Fab fragment from Pichia pastoris that was achieved by co-integration of the genes encoding the heavy and light chains (both under the control of alcohol oxidase promoter) into the genome of the yeast cells. The fed-batch fermentations were carried out in a 5 L scale. Both chains of the Fab were successfully expressed upon methanol induction. The absorbance ($OD_{600}$) of the broth can reach 350~500 at the end of fed-batch phase. After the induction, the expression level of the recombinant Fab (soluble) reached 420~458 mg/L. The recombinant Fab fragment was purified from the crude culture supernatant by ion exchange chromatography and the purity of the recombinant Fab fragment was over 95%. The affinity activities of the crude fermentation supernatant and the purified Fab were analyzed by indirect ELISA, which showed that the purified recombinant Fab fragment had high affinity activity with hepatitis B surface antigen.

• Fisheries and Aquatic Sciences
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• v.4 no.4
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• pp.197-200
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• 2001

To investigate the re-treatment time of Microcotyle sebastis by oral administration of praziquantel, the residue levels of praziquantel in plasma of rockfish Sebastes schlegeli administered orally at a dose of 200 mg/kg B.W. were analyzed by reversed-phase HPLC, and the concentrations of praziquantel in the plasma were correlated with the extermination of M. sebastis. The absorption and depletion of praziquantel in the blood of rockfish were fast and the residual concentrations of praziquantel declined below $4\mu g/mL$ within 24 hr post treatment. Most of worms were exterminated within 3 hr after oral administration of praziquantel, however, a small number of M. sebastis were not killed by the treatment until end of the experiment. Considering fast drop of praziquantel in blood and extermination pattern of M. sebastis in the present results, retreatment at an interval of 9-12 hr would be effective for eradication of M. sebastis.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• autumn
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• pp.450-453
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• 2001

Recently, the government recommend the use of LCC analysis at a feasibility phase by comprehensive countermeasures for efficient public construction projects and comprehensive countermeasures against preventing unconscientious construction. From the end of 1980's, studies of LCC is in progress actively However, it is difficult to put to practical use for lack of a process, a detailed guideline and existing data about LCC analysis. This study proposes a analysis methodology and a cost model can estimate life cycle cost for Buildings. Furthermore, it develops algorithms for computerizing which is able to estimate efficient LCC assessment.

• Journal of the Microelectronics and Packaging Society
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• v.24 no.1
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• pp.17-25
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• 2017

Recently, research and application for a power module have been actively studied according to the increasing demand for the production of vehicles, smartphones and semiconductor devices. The power modules based on the transient liquid phase (TLP) technology for bonding of power semiconductor devices have been introduced in this paper. The TLP bonding has been widely used in semiconductor packaging industry due to inhibiting conventional Pb-base solder by the regulation of end of life vehicle (ELV) and restriction of hazardous substances (RoHS). In TLP bonding, the melting temperature of a joint layer becomes higher than bonding temperature and it is cost-effective technology than conventional Ag sintering process. In this paper, a variety of TLP bonding technologies and their characteristics for bonding of power module have been described.

• Fisheries and Aquatic Sciences
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• v.5 no.4
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• pp.295-301
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• 2002

Experiments were carried out to investigate the effects of metal interaction on the accumulation and elimination of Cd and Cu in the liver of flounder, Paralichthys olivaceus, exposed to sub-chronic Cd (0, 5, 10, 50, 100 ${\mu}g/L$)/Cu $(10 {\mu}g/L)$ mixture. Cd exposure resulted in an increased Cd accumulation in the liver of flounder for exposure periods and concentration, and Cd accumulation increased linearly with exposure time. Cu accumulation profiles were similar to those of Cd. Cd concentration in the liver significantly decreased at the 10th depuration period and elimination rate was $66.20\%,\;86.22\%$ in 50 and $100 {\mu}g/L$at the end of depuration periods, respectively. Although, Cu elimination was similar to Cd elimination phase, Cd elimination rate was higher than that of Cu. Co-relationship of Cd and Cu have a positive correlation coefficient r=0.8620 (P<0.001) and support the strong relationship between Cd and Cu accumulation. As increase with the Cd exposure concentration, there were significant (P<0.001) differences between Cd and Cu accumulation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.1
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• pp.44-48
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• 2003

Experiments were carried out to investigate the elimination of cadmium in tissues (gill, intestine, liver, kidney and muscle) of flounder, Paralichthys olivaceus, after sub-lethal Cd exposure (5, 50, 100 ${\mu}g/L$). During the depuration phase, Cd concentration in the gill decreased immediately following the end of the exposure period. The elimination rates at the end of depuration periods were $18\%$ for 50 ${\mu}g/L$ exposure and $70\%$ for 100 ${\mu}g/L$ exposure. Intestine showed fastest elimination rates of Cd at all concentration. At the end of the depuration period, the Cd concentration was similar to that in the control, Cd elimination in liver significantly decreased after 10 days of depuration period. After 20 days of depuration, the elimination rate was $66.20\%$ in the fish exposed to 50 ${\mu}g/L$ and $86.22\%$ in the fish exposed to 100${\mu}g/L$. The order of cadmium elimination in tissues were decreased intestine>liver${\geq}$gill>>kidney during depuration periods. In this study the gill, intestine and liver showed faster elimination rate of Cd in 50, 100 ${\mu}g/L$-Cd exposure concentration. After the end of the Cd exposure, the Cd concentration in the kidney slowly decreased or remained constant and the Cd concentration in the muscle slowly increased or remained constant.

• Archives of Metallurgy and Materials
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• v.64 no.2
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• pp.481-485
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• 2019

This study was attempted to study for recovery of Li as Li₂CO₃ from cathode active material, especially NCA (LiNiCoAlO₂), recovered from spent lithium ion batteries. This consists of two major processes, carbonation using CO₂ and water leaching. Carbonation using CO₂ was performed at 600℃, 700℃ and 800℃, and NCA (LiNiCoAlO₂) was phase-separated into Li₂CO₃, NiO and CoO. The water leaching process using the differences in solubility was performed to obtain the optimum conditions by using the washing time and the ratio of the sample to the distilled water as variables. As a result, NCA (LiNiCoAlO₂) was phase-separated into Li₂CO₃ and NiO, CoO at 700℃, and Li₂CO₃ in water was recovered through vacuum filtration after 1 hour at a 1:30 weight ratio of the powder and distilled water. Finally, Li₂CO₃ containing Li of more than 98 wt.% was recovered.

• Proceedings of the Korea Database Society Conference
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• 1999.06a
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• pp.253-260
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• 1999

The recycling cell formation problem means that disposal products me classified into recycling part families using group technology in their end of life phase. Disposal products have the uncertainties of product status by usage influences during product use phase and recycling cells are formed design, process and usage attributes. In order to treat the uncertainties, fuzzy set theory and fuzzy logic-based neural network model are applied to recycling cell formation problem far disposal products. In this paper, a heuristic approach fuzzy ART neural network is suggested. The modified fuzzy ART neural network is shown that it has a great efficiency and give an extension for systematically generating alternative solutions in the recycling cell formation problem. We present the results of this approach applied to disposal refrigerators and the comparison of performances between other algorithms. This paper introduced a procedure which integrates economic and environmental factors into the disassembly of disposal products for recycling in recycling cells. A qualitative method of disassembly analysis is developed and its ai is to improve the efficiency of the disassembly and to generated an optimal disassembly which maximize profits and minimize environmental impact. Three criteria established to reduce the search space and facilitate recycling opportunities.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 1999.03a
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• pp.253-260
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• 1999

The recycling cell formation problem means that disposal products are classified into recycling part families using group technology in their end of life phase. Disposal products have the uncertainties of product status by usage influences during product use phase and recycling cells are formed design, process and usage attributes. In order to treat the uncertainties, fuzzy set theory and fuzzy logic-based neural network model are applied to recycling cell formation problem for disposal products. In this paper, a heuristic approach for fuzzy ART neural network is suggested. The modified Fuzzy ART neural network is shown that it has a great efficiency and give an extension for systematically generating alternative solutions in the recycling cell formation problem. We present the results of this approach applied to disposal refrigerators and the comparison of performances between other algorithms. This paper introduced a procedure which integrates economic and environmental factors into the disassembly of disposal products for recycling in recycling cells. A qualitative method of disassembly analysis is developed and its aim is to improve the efficiency of the disassembly and to generated an optimal disassembly which maximize profits and minimize environmental impact. Three criteria established to reduce the search space and facilitate recycling opportunities.