• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.3035-3042
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• 2015

Background: Isorhamnetin (Iso), a novel and essential monomer derived from total flavones of Hippophae rhamnoides that has long been used as a traditional Chinese medicine for angina pectoris and acute myocardial infarction, has also shown a spectrum of antitumor activity. However, little is known about the mechanisms of action Iso on cancer cells. Objectives: To investigate the effects of Iso on A549 lung cancer cells and underlying mechanisms. Materials and Methods: A549 cells were treated with $10{\sim}320{\mu}g/ml$ Iso. Their morphological and cellular characteristics were assessed by light and electronic microscopy. Growth inhibition was analyzed by MTT, clonogenic and growth curve assays. Apoptotic characteristics of cells were determined by flow cytometry (FCM), DNA fragmentation, single cell gel electrophoresis (comet) assay, immunocytochemistry and terminal deoxynucleotidyl transferase nick end labeling (TUNEL). Tumor models were setup by transplanting Lewis lung carcinoma cells into C57BL/6 mice, and the weights and sizes of tumors were measured. Results: Iso markedly inhibited the growth of A549 cells with induction of apoptotic changes. Iso at $20{\mu}g/ml$, could induce A549 cell apoptosis, up-regulate the expression of apoptosis genes Bax, Caspase-3 and P53, and down-regulate the expression of Bcl-2, cyclinD1 and PCNA protein. The tumors in tumor-bearing mice treated with Iso were significantly smaller than in the control group. The results of apoptosis-related genes, PCNA, cyclinD1 and other protein expression levels of transplanted Lewis cells were the same as those of A549 cells in vitro. Conclusions: Iso, a natural single compound isolated from total flavones, has antiproliferative activity against lung cancer in vitro and in vivo. Its mechanisms of action may involve apoptosis of cells induced by down-regulation of oncogenes and up-regulation of apoptotic genes.

• Journal of Korean Society of Steel Construction
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• v.21 no.4
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• pp.421-432
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• 2009

In this study, push-out and static loading tests were conducted to evaluate the behavioral characteristics of composite beams with a perfobond rib shear connector. The shear capacity of the perfobond rib was found to be proportional to its concrete strength, which is in turn affected by the increase in the concrete end-bearing strength and concrete dowel action to resist the shear force. The relative slips of the push-out specimen, however, which was used to assess the ductility of the shear connector, increased to some extent, but it no longer increased when it reached the critical concrete strength because of the flexibility of the transverse rebar in the rib hole. The static-loading-test results revealed a crack on the concrete slab in the composite beam with a perfobond rib on the side of the rib hole and transverse rebar for the applied moment and shear force to the rib hole, depending on the static loading. The shear resistance characteristics of the perfobond rib shear connector were found to resist the shear force from the relative slip on the interface of the composite beam. Thus, the sectional effect of the shear connector to the composite beam with a perfobond rib should be considered when designing the composite beam because the behavior of the composite beam can change owing to the shear connector.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.03a
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• pp.188-197
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• 2010

This paper presents experimental results of a series of 1-g shaking table model tests performed on end-bearing single piles and pile groups to investigate the effect of particle size on the dynamic behavior of soil-pile systems. Two soil-pile models consisting of a single-pile and a $4{\times}2$-pile group were tested twice; first using Jumoonjin sand, and second using Australian Fine sand, which has a smaller particle size. In the case of single-pile models, the lateral displacement was almost within 1% of pile diameter which corresponds to the elastic range of the pile. The back-calculated p-y curves show that the subgrade reaction of the Jumoonjin-sand-model ground was larger than that of the Australian Fine-sand-model ground at the same displacement. This phenomenon means that the stress-strain behavior of Jumoonjin sand was initially stiffer than that of Australian Fine sand. This difference was also confirmed by resonant column tests and compression triaxial tests. And the single pile p-y backbone curves of the Australian fine sand were constructed and compared with those of the Jumoonjin sand. As a result, the stiffness of the p-y backbone curves of Jumunjin sand was larger than those of Australian fine sand. Therefore, using the same p-y curves regardless of particle size can lead to inaccurate results when evaluating dynamic behavior of soil-pile system. In the case of the group-pile models, the lateral displacement was much larger than the elastic range of pile movement at the same test conditions in the single-pile models. The back-calculated p-y curves in the case of group pile models were very similar in both sands because the stiffness difference between the Jumoonjin-sand-model ground and the Australian Fine-sand-model ground was not significantly large at a large strain level, where both sands showed non-linear behavior. According to a series of single pile and group pile test results, the evaluation group pile effect using the p-multiplier can lead to inaccurate results on dynamic behavior of soil-pile system.

• Journal of the Korea Concrete Institute
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• v.21 no.4
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• pp.401-408
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• 2009

A compression lap splice becomes an important issue due to development of ultra-high strength concrete. Current design codes regarding compression lap splice do not utilize merits of the improved strength of ultra-high strength concrete. Especially, a compression lap splice can be calculated longer than a tension lap splice according to the codes because they do not consider effects of compressive strength of concrete and transverse reinforcement. This anomaly confuses engineers in practice. Design equation is proposed for compression lap splice in 40 to 70 MPa of compressive strength of concrete. The proposed equation is based on 51 specimens conducted by authors. Basic form of the equation includes main parameters which are derived from investigating test results. Through two-variable non-linear regression analysis of measured splice strengths, a strength equation of compression lap splices is then derived. A specified splice strength is defined using a 5% fractile coefficient and a lap length equation is constructed. By the proposed equation, the anomaly of lap lengths in tension and compression is got rid of. In addition, the equation has a reliability equivalent to those of the specified strengths of materials.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.10
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• pp.5291-5298
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• 2012

Beta-asarone is one of the main bioactive constituents in traditional Chinese medicine Acorus calamu. Previous studies have shown that it has antifungal and anthelmintic activities. However, little is known about its anticancer effects. This study aimed to determine inhibitory effects on LoVo colon cancer cell proliferation and to clarify the underlying mechanisms in vitro and in vivo. Dose-response and time-course anti-proliferation effects were examined by MTT assay. Our results demonstrated that LoVo cell viability showed dose- and time-dependence on ${\beta}$-asarone. We further assessed anti-proliferation effects as ${\beta}$-asarone-induced apoptosis by annexin V-fluorescein isothiocyanate/propidium iodide assay usinga flow cytometer and observed characteristic nuclear fragmentation and chromatin condensation of apoptosis by microscopy. Moreover, we found the apoptosis to be induced through the mitochondrial/caspase pathway by decreasing mitochondrial membrane potential (MMP) and reducing the Bcl-2-to-Bax ratio, in addition to activating the caspase-9 and caspase-3 cascades. Additionally, the apoptosis could be inhibited by a pan-caspase inhibitor, carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK). When nude mice bearing LoVo tumor xenografts were treated with ${\beta}$-asarone, tumor volumes were reduced and terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assays of excised tissue also demonstrated apoptotic changes. Taken together, these findings for the first time provide evidence that ${\beta}$-asarone can suppress the growth of colon cancer and the induced apoptosis is possibly mediated through mitochondria/caspase pathways.

• Research in Plant Disease
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• v.8 no.1
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• pp.55-58
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• 2002

In July 2001, rotting and shivering flowers of cotton rose (Hibiscus mutabitis) were fecund in the flower beds along the roadsides in Jinju area. The disease first started as water-soaking, dark-green lesions on the petals, and then whole flower was rotted rapidly, Whitish mycelia and monosporous sporangiophore with monosporous sporangiola were formed abundantly on the lesions. Colony appeared as white to pale yellowish brown mycelia on potato dextrose agar medium (PDA). Monosporous sporangiophore was long slender and branched at the apex, each branch bearing a head of sporangiospores. Sporangium was subglobose in shape and was 42.6-114.2$\mu$m in size. Monosporous sporangiola were elliptic, fusiform or ovoid, and brown in color and 12.3~21.6 $\times$8.3~11.6$\mu$m Um in size. Sporangiospores were elliptic, fusiform or ovoid in shape, dark brown or brown in color and 16.3~23.8$\times$8.2~13.6$\mu$m in size, and they had three or more appendages at bipolar end. Zygospores were mostly globose, dark black colored and sized was 46.2-78.4$\mu$min diameter, The fungus grew on PDA between at 15 to 4$0^{\circ}C$, and the optimum temperature was 3$0^{\circ}C$. This is the first report on the flower rot of cotton rose caused by C. cucurbitarum in Korea.

• Journal of the Korean GEO-environmental Society
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• v.9 no.5
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• pp.45-52
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• 2008

The present study has extended OpenSees, which is an open-source software framework DOS program for developing applications to idealize geotechnical and structural problems, for the static analysis of axial load capacity and settlement of single piles in MS Windows environment. The Windows version of OpenSees as improved by this study has enhanced the DOS version from a general purpose software program to a special purpose program for driven and bored pile analysis with additional features of pre-processing and post-processing and a user friendly graphical interface. The method used in the load capacity analysis is the numerical methods based on load transfer functions combined with finite elements. The use of empirical nonlinear T-z and Q-z load transfer curves to model soil-pile interaction in skin friction and end bearing, respectively, has been shown to capture the nonlinear soil-pile response under settlement due to load. Validation studies have shown the static load capacity and settlement predictions implemented in this study are in fair agreement with reference data from the static loading tests.

• Korean journal of applied entomology
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• v.5_6
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• pp.1-7
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• 1968

The present investigation was initiated to find out the possible insect vector of the paulownia witches'-broom virus disease. 1. Among a number of piercing-sucking mouth type insects that were feeding on the witches'-broom infected Paulownia leaves, the tobacco leaf bug, Cyrtopeltis tenuis REUTER, was most abundant. Eggs, nymphs and adults of Cyrtopeltis tenuis were observed from the mid-August through the end of October. 2. It was found that Paulownia witches'-broom virus is transmitted by Cyrotopeltis tenuis. 3. On young Pnulownia plants, first symptom appeared twenty days after exposure to virus-bearing colonies of Cyrtopeltis tenuis. Forty days after exposure to these viruliferous insects, the characteristic symptom of Paulownia witches'-broom was apparent. 4. Paulownia witches' -broom virus was also transmitted by Cyrtopeltis tenuis to calendula and morning glory. No app"rent symptom was observed from tomato, carrot. aster, zinnia, cosmos, soybean and plantago at least until sixty days after exposure to the viruliferous colonies of Cyrtopeltis tenuis. 5. Calendula and morning glory appear to be highly suitable test plants for Paulownia witches'-broom vims because of their relatively short incubation period and distinct symptom appearance.

• Geotechnical Engineering
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• v.5 no.1
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• pp.7-18
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• 1989

In pile load tests on end bearing piles, generally, it is not possible to continue loading to the ultimate load. Thus, the concept of yield load has been introduced for determining design loads Iron the pile load test records. The conventional rules to determine the yield load were not available for evaluation on pile load test records obtained in 6 fields nearby westers 8r Southern Coasts in Korea. A new rule 9.as presented to determine easily the yield load, based on investigations on the pile load test records. The yield load of piles is determined at the infiection point on semi-logarithmic coordinates (P-logS), in which load is plotted in normal scale and settlement is plotted in logarithmic scale. This method may not only save much costs and times but also present safe luorking circumstances for pile load tests in field. It was found that the yield load represented the elastic limit of the pile load-settlement behalf.iota. The ultimate load, which is given at 25.4mm settlement on pile head, was 1.5 times of the yield load. The allowable long-term and short-term load capacities were, respectively, 50% and 75% of the yield load. The safety factors to get the allowable pile capacity were obtained as 2.0~4.0 for the equations to predict the static pile capacity.

• Parasites, Hosts and Diseases
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• v.57 no.6
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• pp.657-664
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• 2019

We identified the echinostome metacercariae in Chinese mystery snails, Cipangopaludina chinensis malleata, from Xiengkhuang Province, Lao PDR with morphologies of adult worms recovered. Total 20 snails were examined with artificial digestion method and then the collected metacercariae were orally infected to a mouse and a rat. Adult worms recovered from experimental animals were observed with a light microscope and a SEM. The metacercariae were round, 125×123 ㎛ in average size, with a moderately thick cyst wall, collar spines distributed in the head collar and excretory granules in 2 canals of excretory tube. Adult flukes (3-week-old in a rat) were elongated, ventrally curved and 5.310×1.023 mm in average size. Head collar distinct, bearing 43 collar spines with 5 end group ones on each side. Oral sucker subterminal, prepharynx very short, pharynx well developed, and esophagus relatively short. Cirrus sac well developed, with a saccular seminal vesicle, and ventral sucker very large. Ovary round and on the median line of the body. Testes tandom and elongated. Eggs operculated, elliptical and 90×57 ㎛ in average size. In the SEM observation, the head crown prominent, with 43 collar spines resembled with horns of younger stag. Scale-like tegumental spines were densely distributed on the surface between the head collar and ventral sucker, and their densities were decreased posteriorly. Conclusively, the metacercariae detected in C. chinensis malleata from Lao PDR were identified as those of Echinostoma macrorchis based on the morphological characteristics of adult worms.