• Microbiology and Biotechnology Letters
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• v.32 no.4
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• pp.322-327
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• 2004

A strong constitutive PJH promoter from Bacillus sp. was applied to overexpress the endoxylanase gene (639 bp) in Bacillus subtilis. The expression plasmid, pJHKJ4, was designed to contain the $P_{JH}$ promoter and open reading frame of endoxylanase including its own promoter. The plasmid was introduced into B. subtilis DB431 and the resulting transformant was grown on LB glucose medium. At the end of cultivation, the endoxylanase activity in the culture supernatant reached about 140 DIm!. The enzyme in the supernatant was concentrated by ultrafiltration (MW cut-off 10 kDa and 30 kDa) and ammonium sulfate precipitation. For the concentration of the enzyme, ultrafiltration was more efficient than 70% ammonium sulfate precipitation. The stabilization of concentrated enzyme solution at $50^{\circ}C$ was examined with various stabilizers such as NaCI, glycerol, polyethylene glycol, sorbitol, and $CaCI_2$. The most effective stabilizers were found to be NaCI and $CaCI_2$.

• The Korean Journal of Nuclear Medicine
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• v.28 no.3
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• pp.338-342
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• 1994

Pulmonary clearance of Tc-99m-DTPA(PCD) has been used for the measurement of polmonary epithelial permeability. It has been reported to be increased not only in variety of polmonary diseases including ARDS, interstitial fibrosis, and smokers, but also in normal subjects on positive end expiratory pressure respirator, or after exercise. It was also noted that decrease of pulmonary blood flow due to pulmonary arterial obstruction results in delayed PCD. Normal range of PCD varies with institutes. We prospectively measured PCD in 17 normals (5 males and 12 females) consisted of staffs and trainees in the department of radiology of Kangnam St. Mary's hospital using original Bark Nebulizer (India). Age ranged from 32 to 43 years. 370 MBq of Tc-99m-DTPA was inhaled in supine position and supine posterior images were subsequently obtained with 1 min/frame, $64{\times}64$ matrix and word mode for 30min. Regions of interest were set on each lung, whole lungs, and upper, middle and lower thirds of right lung, respectively. Best fit regression curve was obtained by least square method from initial 7min after peak activity on each curve and time for half clearance of maximum activity (t1/2) was calculated. Mean t1/2 was $51{\pm}11.2min$ for whole lung. There was no significant difference between t1/ 2 of right and left lungs. Initial uptake was higher in the lower third and t1/2 was shorter in the lower third than in the upper third(P<0.05). We reviewed several reports on PCD and compared our data with the others. In this study, faster clearance in the lower third may be due to the position imaged with or the environment the subjects belong to, and further investigation is under way.

• Journal of Elementary Mathematics Education in Korea
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• v.14 no.1
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• pp.43-64
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• 2010

This study aimed to foster the learning abilities of mathematics, that is, along with the formation of a sure mathematical concept, extending the powers of doing mathematics, and bringing the creativities for 3rd grade elementary school children. In order to achieve these objects, we have executed mathematical classes for two consecutive hours of 16 times using the teaching model of [Learning contents in textbook]$\rightarrow$[The first problem Posing]$\rightarrow$[Problem solving to childrens' posing some problems]$\rightarrow$[Advanced problem posing] to 3rd grade school children during the first semester of 2009. In this paper, we analyzed problems that are made by children focusing on the four fundamental rules +, -, ${\times}$, $\div$ of arithmetic, with the view points of problem's completion, fluencies, flexibilities, buildings of concept, originalities and using materials. As a result of the comparative analysis of the first problems and advanced problems made by the children, the first problems were revealed to be rather better in of problem's completion and fluencies. And the flexibilities were improved in the division and multiplication classes carried on. Setting up the experimental and comparative class, we compared to the scholastic achievement of two classes for the beginning and end in the first semester. In the result, the former was improved in the scholastic achievement more than the latter.

• The Journal of Korean Academy of Prosthodontics
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• v.53 no.4
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• pp.301-309
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• 2015

Purpose: The aim of this study was to evaluate the clinical performance of screw- and cement-retained implant prosthesis (SCP) design in terms of retained preload of abutment screws and cement washout. Materials and methods: Patients with the partial posterior edentulous areas comprised the study group. Implants were placed, and SCPs were delivered after 3 to 6 months healing. Follow-up examinations were performed. The implant survival rate and the prosthetic success rate were evaluated. The retained preload ratio of abutment screws and the prosthetic decementation ratio were measured. Results: Twenty one SCPs (forty three implants)in twenty patients were followed up to 64 months. All of the implants survived during the follow-up period (mean follow-up: 34 months). The prosthetic success rate was 100 % considering no abutment, screw, porcelain or metal frame fractures, as well as no screw loosening. The retained preload ratio of SCPs at the end of follow-up period was 97.61% (${\pm}16.29$) and the decementation ratio was 9.5 %. Conclusion: Within the limitations of this clinical study, SCP design showed favorable short-term clinical performances in respect of screw loosening and cement washout.

• The Korean Journal of Zoology
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• v.8 no.2
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• pp.89-127
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• 1965

1. This report is on the outcome of observation made up the breeding process of Narcissus flycatcher, Muscicapa narcissina zanthopygia, using 15 ness-boxes at Seoul and Kwang Nung Experimental forest in 1964, and the relevant feeding habit was studied by the collar method of wrapping the necks of thenestlings for 50-60 minutes or more in some cases from 1963 to 1964. 2. The Narcissus flycatcheris a common summer resident throughout Korea arriving at the end of April. The earliest data of arrival of this species in Seoul, is April 26, 1964. They migrate southward from the later part of September to early part of October. 3. In a week after their arrival , they start to build nest after a brief courtship. Male and female prepare their nest ina nestboxes in 4 or 5 days. Nests are usually found in the moss, which provide definite frame-work. The inner side of the nest is usually composed of roots of Gramineae or leaves of pine, oak or other broad leave tree. 4. The laying period is generally from the later part of April to the middle of June : and the number of eggs laid is from 3 to 8 , therefore 5 in average : eggs are pink with traces of milky yellow blending of light auburn and orange red spots and chicken-egg-like in shape. 5.The incubation takes place for 7-12 days from the middle part of May to the middle of June, in the order of male and female . The hatching rate tends to increase in proportion to clutch size. 6. The feeding period usually lasts 15 days in average from the early part of June to the middle of July and the feeding urge tends to increase in proportion to brood size. 7. The animals invading into their nest-boxes are red ants, chipmunks, bees , barkbeetles and philippine red-tailed shirkes, etc. 8. On the basis of analysis covering 714 birds in 31 nests, the food they consumed during the nesting period of nestlings were mostly of animal matters composing of : insect larvae form-39. 88% insect adult form 36.26%, insect pupae-0.19%, spider-17.75% and other animal matters 5.92% , Besides a small amount of plant pieces were found . As to the insect larvae, Lepidoptera was the largest in the content followed by Hymenoptera , Orthoptera and Coleoptera. As to the insect adult, Diptera was the largest in the content followed by Coleoptera, and Hymenoptera. In view of forest protection, noxious insect was of 69.38%, useful insect 4.39%, and spider 17.75%. 9. The rich assortment of its food, the ability of acquiring various insect (adults as well as larvae), its destruction of great numbers of harmful insects , and its quick assimilation artificial nesting with the nest-boxes of 2.5-3cm entrance hole employed, allow us to conclude that the flycatcher may be successfully employed in the elimination of fores pests. The above results are only that part obtained near Seoul in the middle of Korea, however, if we would extend the scheme to other broad areas using similar techniques, the nest-boxes could not only be well used by the birds, but also it would help to prevent the increase of harmful. insects.

• Steel and Composite Structures
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• v.1 no.4
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• pp.459-480
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• 2001

The damage suffered by steel structures during the Northridge (1994) and Kobe (1995) earthquakes indicates that the fully restrained (FR) connections in steel frames did not behave as expected. Consequently, researchers began studying other possibilities, including making the connections more flexible, to reduce the risk of damage from seismic loading. Recent experimental and analytical investigations pointed out that the seismic response of steel frames with partially restrained (PR) connections might be superior to that of similar frames with FR connections since the energy dissipation at PR connections could be significant. This beneficial effect has not yet been fully quantified analytically. Thus, the dissipation of energy at PR connections needs to be considered in analytical evaluations, in addition to the dissipation of energy due to viscous damping and at plastic hinges (if they form). An algorithm is developed and verified by the authors to estimate the nonlinear time-domain dynamic response of steel frames with PR connections. The verified algorithm is then used to quantify the major sources of energy dissipation and their effect on the overall structural response in terms of the maximum base shear and the maximum top displacement. The results indicate that the dissipation of energy at PR connections is comparable to that dissipated by viscous damping and at plastic hinges. In general, the maximum total base shear significantly increases with an increase in the connection stiffness. On the other hand, the maximum top lateral displacement $U_{max}$ does not always increase as the connection stiffness decreases. Energy dissipation is considerably influenced by the stiffness of a connection, defined in terms of the T ratio, i.e., the ratio of the moment the connection would have to carry according to beam line theory (Disque 1964) and the fixed end moment of the girder. A connection with a T ratio of at least 0.9 is considered to be fully restrained. The energy dissipation behavior may be quite different for a frame with FR connections with a T ratio of 1.0 compared to when the T ratio is 0.9. Thus, for nonlinear seismic analysis, a T ratio of at least 0.9 should not be considered to be an FR connection. The study quantitatively confirms the general observations made in experimental results for frames with PR connections. Proper consideration of the PR connection stiffness and other dynamic properties are essential to predict dynamic behavior, no matter how difficult the analysis procedure becomes. Any simplified approach may need to be calibrated using this type of detailed analytical study.

• Trans-
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• v.3
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• pp.1-28
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• 2017

As the field of film aesthetics expands, the concept of classical the mise-en-scene expands too its scope. This paper examines the concept of mise-en-scene through spatial connections. The primary goals of this study are understanding the relation between the frame and the shot, the continuity between the scene and the sequence, and the aesthetic dimension to the plan-sequence. In modern cinema theory, the elements of mise-en-scene are more emphasized in terms of efficiency, but this study is concerned that the classical aesthetic system is still important. To this end, we examine the concept of plan-sequence, which was called the best technique of past visual realism aesthetics, is utilized in digital cinema. Finding significance for the modern use of plan-sequences is a secondary goal of this paper and we expect it to be a stepping stone to search for the aesthetics of digital cinema. The plan-sequence is one of the important factors that can be evaluated in the preparation and application of the event for the movie director. In the real world where the film itself is used commercially, digital film will have a conceptual tool that can utilize allegorics of 'geopolitical unconsciousness' through plan-sequences.

• Journal of the Korea institute for structural maintenance and inspection
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• v.19 no.5
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• pp.56-66
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• 2015

This study aims at developing a new seismic resistant method by using precast concrete wall panels for existing low-rise, reinforced concrete beam-column buildings such as school buildings. Three quasi-static hysteresis loading tests were performed on one unreinforced beam-column specimen and two reinforced specimens with U-type precast wall panels. Seismic resistant test of anchored and welded steel plate connections manifested an average of 2.8 times increase in the maximum loading (average 591.8 kN) in comparison to unreinforced beam-column specimen. The maximum drift ratios were also shown between 1.4% and 2.7%. An analytical study was performed while assuming the RC column on the right side and the vertical element of the reinforced PC panel to behave in completely composite manner and the RC column on the left side and PC panel to behave in completely non-composite manner when loading was exerted from upper right end of RC frame of specimen to its left side. It was found with the assumptions that the overall flexural behavior in principle agreed with the experimental result.

• Journal of Legislation Research
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• no.44
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• pp.103-153
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• 2013

This article aims at the legislation of User-Protection Act in area of ICT. In these days telecommunication and broadcasting are getting more and more convergent. The paradigm of ICT is turning over from the service provider to the end-user. User protection has been in each erea of ICT (C-P-N-D) individually regulated. In the area of telecommunication it is important to protect the interest of user, who stands in contract with the service provider. And in area of broadcasting it is important to protect the interest of viewers, who stands "gratis" with the broadcasters without any contracts. For the more efficient user-protection it is also necessary to make a dedicated organization under KCC(Korean Communications Committee). In this early year the government organization was divided into MSIP(Ministry of Science, ICT and future planing) and KCC. The user-protection act will be very important instrument of ICT regulation in the era of creative economy. It is necessary to establish a new frame act of user protection. It is also necessary to make start to establish a new system of user education in erea of ICT. It is strongly expected the new act will be a turning point of ICT development in Korea.

• The Journal of the Korea Contents Association
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• v.18 no.12
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• pp.405-420
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• 2018

As the intelligent vehicle market continues to develop relevant technologies and services for consumers, it is necessary to understand the characteristics of potential consumers. The purpose of this study is to identify and understand the types of potential consumers of intelligent vehicle using the Q-methodology. A Q-frame was constructed using thirty six statements from intelligent vehicle related literature concerning core technology, technology acceptance and personal consumption value, legal system and policy and social awareness. Q-sorting and in-depth interviews were conducted using thirty nine P-samples snowballed. Analysis produced four types of potential consumers for intelligent vehicle: Smart Car Consumer, Reasonable Consumer, Safety Car Consumer, and Smart Device Consumer. Smart Car Consumer value the vehicle capability of intelligent vehicle as most important while Reasonable Consumer focus upon the economics of intelligent vehicle. Safety Car Consumer recognize the safety of intelligent vehicle as most important while Smart Device Consumer highly value the IT functions provided by intelligent vehicles. Across these four different types of consumers, preventing injuries of intelligent vehicle drivers turned out to be the most common critical factor in assessing intelligent vehicle. Implications for the intelligent vehicle market is discussed at the end with further studies needed.