• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.97-97
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• 2003

For many animals the centrosome consists of a pair of centrioles and surrounding pericentriolar materials (PCMs). PCMs have been known to play roles during cell division. It is known that centrioles are necessary to assemble centrosomal components. However, many types of oocytes undergo meiosis without centrioles. It is known that in nonmurine mammalian species, the sperm introduces an intact proximal centriole unlike sea urchin where two centrioles are introduced. In case of mouse sperm, the presence of centrosome is not clear In this study, a monoclonal antibody was developed to investigate centrosome during mouse germ cell and early embryo development. Results of immunostaining and Western blotting in CHO cells suggest that the monoclonal antibody recognizes a nuclear and centrosomal protein, thus called NCP. The NCP monoclonal antibody was used to screen a cDNA expression library prepared from 12.5 mouse brain to isolate NCP gene. Nucleotide size of NCP gene obtained from immunoscreening was about 5.5kb. It is determined that the NCP may be closely related with pericentriolar material -1 gene (Pcm-1) from the result of sequencing analysis. The molecular weight, 66kDa, calculated by known DNA sequence in database is consistent with that of detected from Western blotting using CHO cell lysates. Therefore, it is assumed that NCP may be alternative splicing form of Pcm-1 of which molecular weight is 228kDa. In mouse oocytes, NCP was distributed in nucleus as in CHO cells. It was shown that the NCP was localized around neck region, probably the centrosome in mouse neck region. Interestingly, dramatic change in distribution of NCP was also shown in male germ cell development. Finally, we observed the cellular distribution of NCP during early embryo development. NCP was detected in nucleus as well as centrosome foci. It is suggested that the centrioles reassembly we occurring in blastocysts and then affects the distribution of NCP.

• Korean Journal of Ichthyology
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• v.22 no.4
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• pp.217-224
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• 2010

An investigation was carried out to obtain basic information needed to develop methods for artificial propagation and conservation of the mottled skate Raja pulchra, an important food resource in western Korea that has declined from overfishing. In this paper we provide evidence of multiple spawning and describe properties of the ovary, and morphology of the egg capsule and the fully-formed offspring. The vitellogenic follicles in the ovary was $179.8{\pm}57.1$ (54~247) and can be classified into five size groups, which the last group of ova are considered as the moving to the capsule gland where fertilization and encapsulation of ova take place. The morphology of the egg capsule of R. pulchra is unique among the species of the family Rajidae and showed multi-embryo characteristics, having two to six yolks in each capsule. The adaptive morphological changes of larvae developing inside the egg capsule are described based on specimens extracted from the capsule.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.1
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• pp.26-34
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• 1996

Copulation process, fertilization and gestation of the viviparous teleost surfperch, Ditrema temmincki were investigated by using photomicroscopy. Samples were collected from the vicinity of Suyoung Bay, Pusan, Korea from May 1992 to August 1993. During the copulation period, the copulatory organs were protruded at the base part of right and left soft ray of the anal fin in mature male. Secondary sexual characteristics index (SSCI) of male participated in copulation was above 3.5. Mature oocytes appeared only in the female containing sperms in the ovarian cavity. Transport of the spermatozoa into the ovarian cavity during copulation belonged to a spermatophore type. After copulation, spermatozoa remained in the ovarian cavity for about one month untill fertilization. Fertilization occured within the follicular cavity. Fertilized eggs were released into the ovarian cavity where they developed during gestation period. Developmental sequence of the female was as following: fertilization-ovulation-hatching-parturition. Right before parturition, the total length (TL) of the embryo was about 63.0 mm. When TL of maternal body was 20.0 cm, the mean numbers of the embryo were 18. The numbers of the embryo were positively related to the maternal body size.

• Korean Journal of Plant Resources
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• v.29 no.3
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• pp.339-346
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• 2016

This study was carried out to develop an effective seed propagation method for Thalictrum rochebrunianum var. grandisepalum (H. Lev.) Nakai by analyzing seed dormancy types and germination characteristics. Seeds were collected between September to October at Gangwon province, and well-selected seeds were used while being dry-stored at 4±1℃. The seed size ranged 4.52 × 1.58 ㎜ and the weight of thousand seeds were 1,603.5 ± 0.02 ㎎. The moisture content was 7.2%. Seeds were achene type, and morphology characters showed an elliptical shape and rough texture, and light brown in color. Moist-chilling treatment was conducted for dormancy breaking because the seeds had an undeveloped embryo of liner type. The embryo had developed during a moist-chilling period, constantly, and fully developed in 10 weeks. Consequently, it seemed to be non-deep complex or intermediate complex type of morphophysiological dormancy, and embryo dormancy was broken by wet-chilling for 10 weeks. After 10 weeks of wet-chilling treatment, seed germination began. Germination percentage was higher in dark condition raher than light condition and recorded the maximum at 25℃ in the dark (16.3%). A pre-soaking treatment with a combined plant growth hormones promoted germination and shortened T₅₀. Specifically, seed germination of 84.5% was achieved by pre-soaking of seeds with a combined solution of 500 ㎎/L GA₃ and 10 ㎎/L kinetin for 24 h after a wet-chilling treatment for 10 weeks. Thus the effect of plant growth hormones coupled with chilling temperature on seed breaking dormancy provide asubsequent growth of seedlings for successful plantation.

• Korean Journal of Animal Reproduction
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• v.21 no.1
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• pp.31-37
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• 1997

Ultrasonographic diagnosis of genital disease and early pregnancy diagnosis was performed in Korean native cattle. The size of ovarian follicle in preovulation, luteal stage and follicular cyst was 18.9, 9.2 and 27.6 mm, respectively, and the thickness of follicular wall was 2.3, 1.8 and 2.8 mm, respectively. The size of corpus luteums in formation stage, activity stage, regression stage, cystic corpora lutea and luteal cyst was 6.2, 11.3, 8.6, 26.7 and 25.9 mm, respectively. The thickness of luteal wall in cystic corpora lutea and luteal cyst was 8.4 and 4.9 mm, respectively. The size of embryo or fetus on day 25, 27, 30, 35, 40, 45 and 50 was 0.8, 0.9, 1.3, 1.5, 2.2, 2.8 and 3.8 cm, respectively. The size of amniotic vesicle on day 25, 27 and 30 was 1.2, 2.1 and 3,0 cm, respectively. The diameter of pregnant uterus on day 25 and 27 was 7.0 and 7.8 cm, respectively. It was concluded that the ultrasonographci values determined in this study can be used as references for the treatment of genital disease and early pregnancy diagnosis in Korean native cattle.

• Journal of Embryo Transfer
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• v.27 no.2
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• pp.81-85
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• 2012

This study investigated the effect of birth weight on the fertility of Holstein heifers. Growth parameter (body weight) was measured at birth. Calves were analyzed as three subgroups: low (L), average (A) and high (H) birth weight (BW) calves. LBW calves were born 10 kg lighter than HBW calves. Fertility data collected included age at first breeding (AFB), number of services per conception, pregnancy rate to first artificial insemination (AI), and age at first calving (AFC). Primiparous calves in HBW are smaller compared to multiparous cows (18.3% versus 48%). Although not significantly different, LBW offspring appeared to breed faster over the service period compared with the ABW offspring that bred three weeks later on the average. The mean pregnancy rate to first AI for LBW heifers was higher (60%) than ABW (48%) and HBW (45%). HBW cows required more services per conception (2.1) than the LBW (1.7) and ABW (1.9). There were significant differences (p<0.05) in age at first calving among the different calving birth weights. Smaller birth size did not have any subsequent adverse effects on fertility. HBW offspring were more likely or tend to have worse fertility parameters.

• Journal of Embryo Transfer
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• v.31 no.2
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• pp.137-143
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• 2016

This study was conducted to evaluate a visual surveillance system. The advancement of recording technology and network service make it easy to record and transfer the videos. Moreover, progressed recognition technology help to make a distinction each other. Cows show distinguishing behaviors during their estrus period. The mounting is one of the behaviors. The result was different depending on the breed of the cows and the size of the farm. In the case of Korean native cattle, the estrus detection rate was 71.15%, however, dairy cows, the estrus detection rate was 39.38%. At the farms having below 6 modules, the estrus detection rate was 87.41%. On the other hand, at the farms having over 6 modules, the estrus detection rate was 77.78%. With the proper progress, the visual surveillance system can be used to detect heat detection.

• Journal of Embryo Transfer
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• v.17 no.3
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• pp.195-201
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• 2002

This study was to assess the developmental capacity of oocytes matured in vitro after 20, 15, 10, 5 and 0 days of organ culture when ovaries were isolated from juvenile mice at 0-, 5-, 10-,15- and 20-day old, respectively, and to develop in vitro culture system that observed a view to morphology of ovaries and nucleus maturation of oocytes. The size of ovaries decreased 35.9%, 8.7%, 1.2% and 14.4% after 20, 15, 10, 5 days of organ culture when the ovaries were isolated from 0-, 5-, 10 and 15-day old mice, respectively. After organ culture, the recovery rates, diameters of oocytes and the number of oocytes progressed from GV to MII were increased as increasing age of mice.

• Current Research on Agriculture and Life Sciences
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• v.6
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• pp.1-6
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• 1988

The experiment was conducted to study on the physiology of developing embryo and endosperm degradation during germination of Zea mays seeds treated with GA 20 and B-Nine 5000 ppm. Data obtained can be summerized as follows : 1. Swelling of seminal root increased the section of GA treatment more or less, on the other hand, the section of B-9 treatment inhibited slightly. 2. According to elapsing of seeding date, epithelial cell of Scutellum expansed in size, and the space of cell increased, that degree was enlarged as follows ; the section of GA, the section of Control, and the section of B-9. 3. According to the elapsing of seeding date, the formation of vascular organization embryo became clearer little by little, the lignification of vascular B-9 treatment section rather higher than the other section. 4. The degradation of Starch Grain is composed of near part of epithelial cell of Scutellum, the shape of degradation radiate from element of a disk shape, and the speed of degradation is the section of GA, the section of Control, the section of B-9 in turn.

• Toxicological Research
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• v.17 no.1
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• pp.27-32
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• 2001

Korean ginseng products have been fumigated with ethylene oxide (EO) for sterilization and prolongation of storage periods. However, there had been controversies indicating that consumption of EO treated foods might cause harmful effects in human. In Korea, the use EO gas for food treatment was banned in 1991. Since then, irradiation technique has been developed as an alternative. This study was carried out to evaluate the safety of irradiated ginseng on embryo and fetal developmental toxicity effects in rats. Either EO gas fumigated or $\gamma$-irradiated ginseng was administered to pregnant Wistar rats by oral gavage from gestational day 7 to 17. The amount of irradiation used in this study was 5, 10 and 30 kGy, respectively. There were no treatment related changes of dams in deaths, clinical signs, food consumption and body/organ weight. No treatment related changes in implantation ratio, litter size, sex ratio and body/organ weight of fetuses were observed. Also, no F1fetuses with external, visceral, head and skeletal mal-formation were observed. The results of this study showed that $\gamma$-irradiated ginseng, up to 30 kGy, has no adverse effects on fetal development of rats.