• Title/Summary/Keyword: Elsholtzia

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Methanol extract of Elsholtzia fruticosa promotes 3T3-L1 preadipocyte differentiation

  • Deumaya Shrestha;Eunbin Kim;Krishna K. Shrestha;Sung-Suk Suh;Sung-Hak Kim;Jong Bae Seo
    • Journal of Animal Science and Technology
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    • v.66 no.1
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    • pp.204-218
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    • 2024
  • Elsholtzia fruticosa (EF) is present in tropical regions throughout South Asian countries as well as the Himalayas. Although it has been used as a traditional medicine to treat digestive, respiratory, and inflammatory issues, its effect on preadipocyte differentiation is unknown. In this study, we examined the effects of a methanol extract prepared from EF on the differentiation of 3T3-L1 preadipocytes. Cell differentiation was assessed by microscopic observation and oil-red O staining. The expression of adipogenic and lipogenic genes, including PPARγ and C/EBPα, was measured by western blot analysis and quantitative real-time polymerase chain reaction (qRT-PCR), to provide insight into adipogenesis and lipogenesis mechanisms. The results indicated that EF promotes the differentiation of 3T3-L1 preadipocytes, with elevated lipid accumulation occurring in a concentration-dependent manner without apparent cytotoxicity. EF enhances the expression of adipogenic and lipogenic genes, including PPARγ, FABP4, adiponectin, and FAS, at the mRNA and protein levels. The effect of EF was more pronounced during the early and middle stages of 3T3-L1 cell differentiation. Treatment with EF decreased C/EBP homologous protein (CHOP) mRNA and protein levels, while increasing C/EBPα and PPARγ expression. Treatment with EF resulted in the upregulation of cyclin E and CDK2 gene expression within 24 h, followed by a decrease at 48 h, demonstrating the early-stage impact of EF. A concomitant increase in cyclin-D1 levels was observed compared with untreated cells, indicating that EF modulates lipogenic and adipogenic genes through intricate mechanisms involving CHOP and cell cycle pathways. In summary, EF induces the differentiation of 3T3-L1 preadipocytes by increasing the expression of adipogenic and lipogenic genes, possibly through CHOP and cell cycle-dependent mechanisms.

Biological Activity of Flavor Components Extracted from Elsholtzia ciliata and Elsholtzia splendens (향유와 꽃향유 향기성분의 생리활성 검정)

  • Jeong Jae-Hoon;Sohn Hyung-Ok;Shin Han-Jae;Hyun Hak-Chul;Lee Dong-Wook;Lim Heung-Bin
    • Journal of the Korean Society of Tobacco Science
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    • v.27 no.1 s.53
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    • pp.19-30
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    • 2005
  • This study was to evaluate the biological activity of flavor components extracted from E. ciliata and E. splendens in order to survey the possibility applicable to tobacco and food industry. Flavor components were extracted with dividing into three parts; essential oil, absolute, oleoresin. In the nonenzymatic lipid peroxidation system, the inhibition rate($\%$) of essential oil were $67.3\;\pm\;20.7\%,\;58.1\;\pm\;19.3\%$ at the concentration of 50 ${\mu}g/mL$ of E. ciliata and E. splendens, respectively. The inhibition rate($\%$) of the oleoresin in E. ciliata was higher than one in E. splendens. In the enzymatic lipid peroxidation system, the inhibition rate($\%$) of essential oil and oleoresin was$14.28\;\pm\;2.38\%,\;and\;65.93\;\pm\;0.01\%,\;and\;was\;22.58\;\pm\;2.84\%\;and\;40.73\;pm\;6.04\%$. The oleoresin of two species were showed above $90\%$ of the inhibition rate($90\%$) against autooxidative lipid peroxidation system. $EC_{50}$ values in neutral red uptake assays 24 h of exposure times were $23.3\;{\mu}g/mL,\;341.0\;{\mu}g/mL\;and\;17.2\;{\mu}g/mL$ in essential oil, absolute and oleoresin from E. ciliata respectively, and were $46.4\;{\mu}g/mL,\;681.7\;{\mu}g/mL\;17.6\;{\mu}g/mL$ in three extractions of E. splendens. Oleoresin of two species showed high rate in the cytotoxic effect by neutral red uptake assay. Absolute and oleoresin did not show antibiotic and mutagenic activity. On the contrary, essential oil with over 500 ug/plate showed antibiotic and mutagenic activity in Ames test. Essential oil and oleoresin have a prolongating effect the ciliostasis of rat trachea. This results indicate that flavor components extracted from E. ciliata and E. splendens can be considered to be toxicological safe and to be the possibility applicable the cigarette, food and drug industry as a flavor for expectoration.

Volatile Compounds of Elsholtzia splendens (꽃향유의 휘발성 향기성분)

  • Lee, So-Young;Chung, Mi-Sook;Kim, Mi-Kyung;Baek, Hyung-Hee;Lee, Mi-Soon
    • Korean Journal of Food Science and Technology
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    • v.37 no.3
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    • pp.339-344
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    • 2005
  • Volatile compounds, isolated from Elsholtzia splendens using simultaneous steam distillation extraction (SDE) and headspace solid phase microextraction (HS-SPME), were analyzed by gas chromatography/mass spectrometry(GC-MS). Twenty-nine compounds, comprising 3 aldehydes, 7 alcohols, 11 hydrocarbons, 5 ketones, and 3 miscellaneous ones, were tentatively identified from volatile compounds of Elsholtzia splendens flowers. From leaves, 30 compounds, comprising 3 aldehydes, 6 alcohols, 11 hydrocarbons, 6 ketones, and 11 miscellaneous ones, were tentatively identified. Volatile compounds extracted by HS-SPME in E. splendens flowers were 3 alcohols, 18 hydrocarbons, 3 ketones, and 2 miscellaneous ones. In leaves, 31 compounds, comprising 7 alcohols, 15 hydrocarbons, 7 ketones, and 2 miscellaneous ones, were tentatively identified. Major volatile compounds identified by SDE and HS-SPME were naginataketone and elsholtziaketone, which were identified as aroma-active compounds, representing characteristic aroma of E. splendens.

The chemical composition and biological activities of volatile flavor components of Elsholtzia splendens (꽃향유 전초의 향기성분 분석과 생리활성 평가)

  • Jeong, Jae Hoon;Lim, Heung Bin
    • Analytical Science and Technology
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    • v.18 no.6
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    • pp.500-510
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    • 2005
  • This study was conducted to investigate the chemical composition of essential oil, absolute and oleoresin isolated from Elsholtzia splendens and their biological activities. Yields of essential oil, absolute and oleoresin extracted from Elsholtzia splendens were 0.28%, 12.45% and 9.95%, respectively. The major component was 2-cyclohexen-1-one in essential oil, methyl linolenate in absolute and 9,12,15-octadecatrienoic acid in oleoresin. Essential oil and oleoresin showed the inhibitory activities in enzyme-dependent, enzyme-independent and autooxidative lipid peroxidation system. $EC_{50}$ values in nuetral red uptake (NRU) assays for the exposure times of 24 h were $46.4{\mu}g/ml$, $681.7{\mu}g/ml$ and $17.6{\mu}g/ml$ in essential oil, absolute and oleoresin, and oleoresins showed the cytotoxic effect at the only high dose. Any mutagenic and antibiotic activity did not show in absolute and oleoresin, but, there were mutagenic and antibiotic activities only when treated with essential oil $500{\mu}g/ml$ above in Ames test. Essential oil and oleoresin might be somewhat effective in prolongating the ciliostasis of rat trachea.

Study of Dried Korean Native Plants Using for Floral Clusters (건조 자생식물 종별 꽃다발 이용 가능성 검토)

  • Sohn, Kwanhwa;Kwon, Hye Jin
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.266-274
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    • 2008
  • Korean native plants were investigated for their suitability to be used for floral clusters after drying. Floral clusters which were made in semi-sphere with a hand-tied method and can be fit in $22{\times}22{\times}37cm$ paper boxes were made with 37 species. Among 37 species, the 14 species which were easy to dry, to make floral clusters, and have not been used in other countries, were Echinochloa crus-galli var. frumentacea, Imperata cylindrica var. koenigii, Muhlenbergia huegelii, Phleum pratense, Setaria glauca, and Setaria viridis in Gramineae, Artemisia capillaris, Artemisia montana, and Chrysanthemum indicum in Compositae, Agastache rugosa, Elsholtzia ciliata, Elsholtzia splendens in Labiatae, Carex neurocarpa in Cyperaceae, and Vitex rotundifolia in Verbenaceae were used with leaves, flowers, and fruits. The suitable plants for a floral cluster in $22{\times}22{\times}37cm$ paper box, a wreath in $22{\times}22{\times}6cm$ paper box, and a flower arrangement in $22{\times}22{\times}22cm$ paper box, which were made to a set, were 10 species, that is Phragmites communis, Setaria glauca, Setaria viridis, Artemisia capillaris, Artemisia montana, Chrysanthemum indicum, Elsholtzia ciliata, Elsholtzia splendens, Mosla punctulata, and Vitex rotundifolia.

Analysis of Essential Oil Components using Elsholtzia splendense Nakai, a Fragrant Plant Distributed in Korea (자생 방향성 식물 꽃향유의 정유성분 분석)

  • Jung, J.H.;Park, N.B.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.23 no.1
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    • pp.5-12
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    • 2021
  • In order to extract the essential oil contained in the Elsholtzia splendense Nakai, a fully-bloomed individual was collected and the living body was used for the experiment. Plants were divided into 0.7kg of flowers and 1.5kg of leaves + stems, and extracted by parts and used for scent pattern analysis, and extracted after adding 5kg of outpost for the analysis of essential oil components. Essential oil extraction was performed using the SDE method improved by Schultz et al. (1977), and the extraction time was limited to 1 hour after the material started boiling. The extracted essential oil component was analyzed for fragrance pattern analysis using an e-nose, and the results of analyzing the substance of the essential oil component by GC-MS are as follows. 1. As for the fragrance pattern analysis, in the case of essential oils extracted from flowers, the scent quality was the best at 10-20 minutes, and the scents extracted from stems and leaves were somewhat of poor scent quality, but the fragrance was good at 10-40 minutes. The intensity of scent was the strongest in 10-20 minutes, and the intensity of incense was high even in 30-40 minutes. The scent extracted from the stems and leaves was generally not strong, but appeared high in 10 to 20 minutes. 2. There were 40 kinds of essential oils contained in Elsholtzia splendense Nakai oil. Among them, Mequinol, Benzene, 1,2,3,4-tetramethyl, Elsholtziaketone, and Dehydroelsholtziaketone were identified.

Screening of Chinese Herbal Medicines with Inhibitory Effect on Aldose Reductase (VII) (중국 약용식물 추출물의 알도즈 환원 효소 억제 효능 검색 (VII))

  • Lee, Yun Mi;Kim, Young Sook;Kim, Joo Hwan;Kim, Jin Sook
    • Korean Journal of Pharmacognosy
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    • v.44 no.2
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    • pp.161-167
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    • 2013
  • Aldose reductase (AR) has been shown to play an important role in the development of the diabetic complications. To discover novel treatments for diabetic complications from natural sources, 59 Chinese herbal medicines have been investigated for inhibitory activities on AR. Among them, 10 herbal medicines, Catalpa fargesii (stem and leaf), Saussurea Laniceps(whole plant), Alnus nepalensis(stem and leaf), Swertia macrosperma (whole plant), Woodfordia fruticosa (stem and leaf), Elsholtzia bodinieri (whole plant), Elsholtzia fruticosa (whole plant), Rosa multiflora (fruit), Nardostachys chinensis (whole plant), Eurya groffii (stem and leaf) exhibited a significant inhibitory activity compared with 3,3-tetramethyleneglutaric acid (TMG) as positive control. Particularly, 4 herbal medicines, C. fargesii (stem and leaf), S. Laniceps (whole plant), A. nepalensis (stem and leaf), S. macrosperma (whole plant) showed two times more potent inhibitory activity than TMG ($5.37{\mu}g/ml$).

Chemical Composition and Biological Activities of Elsholtzia ciliata (Thunb.) Hylander (향유의 향기성분 분석 및 생리활성 검정)

  • Jeong, Jae-Hoon;Lim, Heung-Bin
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.6
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    • pp.463-472
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    • 2004
  • This study was carried out to investigate the chemical composition of essential oils, absolutes and oleoresins isolated from Elsholtzia ciliata and the biological activities of them. Yields of essential oils, absolutes and oleoresins were 0.34%, 11.33% and 15.24%, respectively. The major component was naginate ketone in essential oils, methyl linolenate in absolutes and 9,12,15-octadecatrienoic acid in oleoresins. Eseential oils and oleoresins showed the inhibitory activities in enzyme-dependent, enzyme-independent and autooxidatve lipid peroxidation systems. $EC_{50}$ values in neutral red uptake assays 24 h of exposure times were $23.3\;{\mu}g/m{\ell}$, $341.0\;{\mu}g/m{\ell}$ and $17.2\;{\mu}g/m{\ell}$ in essential oils, absolutes and oleoresins, respectively, and essential oils and oleoresins showed the cytotoxic effect at the only high dose. Absolutes and oleoresins did not show antibiotic and mutagenic activities. On the contrary, essential oils with over $500\;{\mu}g/plate$ showed antibiotic and mutagenic activities in Ames test. Essential oils and oleoresins have a prolongating effect the ciliostasis of rat trachea.