• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.107-110
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• 2000

Elicited cells with methyl jasmonate continued to produce benzophenanthridine alkaloids throughout medium changes in suspension cultures of Eschscholtzia californica. Large increases in alkaloid production were observed by re-elitations with medium changes. The total alkaloid production increased during the successive elicitation steps reaching a maximum level on the 4th elicitation. The highest total alkaloid produced was 250 mg/I, which was 20fold higher than that of the single elicitation and 4-fold higher than that of the normal culture without elicitation. The large increases in alkaloid production in successive re-elicitations with medium changes are believed to be caused by the accumulation of the signal transduction compound, jasmonate.

• 사상체질의학회지
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• 제14권3호
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• pp.114-131
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• 2002

1. PURPOSE : The purpose of this study is to investigate the effect of Chungpyesagantang on LPS induced Arthritis in Mice. 2. METHOD : All the BALB/C Mice used in this study were 4wks of age at the start of the experiment. The experimental model of Arthritis was induced by injectection of $300{\mu}g/kg$ LPS in mice knee joint. The experiment was compare daily CS treatment group after Arthritis elicitation with Arthritis elicitated group at day 4, 7, 14 after Arthritis elicitation. 3. RESULTS 1) The hyperplasia of synoviocytes of CS treatment group after Arthritis elicitation is soften than Arthritis elicitated group. 2) The aggregation of collagen fibers CS treatment group after Arthritis elicitation is decreased than Arthritis elicitated group. 3) The distribution of TUNEL positive cells(apoptotic cell) of CS treatment group was remarkably increased than Arthritis elicitated group. 4) The distribution of $TNF-{\alpha}$, $NF-{\kappa}B\;p50$, COX-2 positive cells of CS treatment group after Arthritis elicitation in synovial membrane was decreased than Arthritis elicitated group. 5) The distribution of $IL-2R-{\alpha}$, ICAM-1 positive cells of CS treatment group after Arthritis elicitation in apical surface of synovial membrane was decreased than Arthritis elicitated group. 6) The distribution of $NF-{\kappa}B\;p50$, $IL-2R-{\alpha}$ in common iliac lymph node of CS treatment group after Arthritis elicitation positive cells was decreased than Arthritis elicitated group. 4. CONCLUSION : As a result of these experimental results, it may be concluded that Chungpyesagantang used for treatment of LPS induced Arthritis in Mice. Inflamation activity in CS treatment group after Arthritis elicitation was decreased than Arthritis elicitated group.

• Journal of Microbiology and Biotechnology
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• 제9권3호
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• pp.358-360
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• 1999

The effect of pectinase on menthol production by Mentha piperita in shake flasks was investigated. The optimum concentration of pectinase and period of elicitation for menthol production were 15 U/1 and 9 days, respectively. Pectinase elicitation at 15 U/1 for 9 days using a Lin-Staba medium with 2,4-dichlorophenoxyacetic acid (2,4-D) enhanced menthol production 37-fold (211.5 ㎎ menthol/l) with the specific menthol concentration (menthol concentration per unit weight of cells) of 27.5 ㎎/g dry cell weight (DCW). Our results also indicate that pectinase elicitation may activate the conversion of pulegone to menthol.

• Journal of Microbiology and Biotechnology
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• 제3권4호
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• pp.238-243
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• 1993

In the suspension cultures of Eschscholtzia californica, phytohormone effects showed that alkaloid production was increased by IAA treatment without kinetin in both volumetric and specific way. Kinetin, however, suppressed alkaloid accumulation. Addition of ethephon inhibited cell growth. However, it enhanced the alkaloid production significantly in both volumetric and specific way. IAA promoted alkaloid production during elicitation. The highest alkaloid accumulation was observed at 5 $\mu$ M of IAA. Ethephon also enhanced alkaloid production during elicitation. The highest alkaloid formation was observed at 460 mg/l of ethephon with elicitation. Elicitation with ethephon, however, altered cell growth and the pattern of benzophenanthridine alkaloids production.

• 한국컴퓨터정보학회논문지
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• 제24권3호
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• pp.95-104
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• 2019

Crowdsourcing is growing its interests in designing where various designers work independently to a given design task. Recent researchers discovered that collaboration by sharing designs among designers helps to produce high-quality designs. However, design task may still be hard even with that collaboration in case the requirements are not well-defined. Most customers sometimes do not know what they really want and do not know how to clearly define the requirements. Consequently, the lack of requirements creates issues on designers, such as spending much time and effort on collecting requirements alone or from the customers. The designers even end up missing important necessities to complete their tasks. To address this issue, we proposed a collaborative requirements elicitation method that supports designers who are working on the same task. We developed CREFD (Collaborative Requirements Elicitation For Designers and Developers) tool to enable designers collaboratively provide requirements, identify dependencies, add annotations and votes to the provided requirements. We performed the hypothetical and empirical evaluations to test and compare the proposed method with one of the existing elicitation methods, the results show that the proposed method helps in collecting accepted and well-organized requirements better than individual requirements elicitation.

• Nuclear Engineering and Technology
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• 제53권10호
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• pp.3298-3313
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• 2021

The objective of this study is to estimate the occurrence probabilities of severe accident phenomena based on a fuzzy elicitation technique. Normally, it is difficult to determine these probabilities due to the lack of information on severe accident progression and the highly uncertain values currently in use. In this case, fuzzy set theory (FST) can be best exploited. First, questions were devised for expert elicitation on technical issues of severe accident phenomena. To deal with ambiguities and the imprecision of previously developed (reference) probabilities, fuzzy aggregation methods based on FST were employed to derive the occurrence probabilities of severe accidents via four phases: 1) choosing experts, 2) quantifying weighting factors for the experts, 3) aggregating the experts' opinions, and 4) defuzzifying the fuzzy numbers. In this way, this study obtained expert elicitation results in the form of updated occurrence probabilities of severe accident phenomena in the OPR-1000 plant, after which the differences between the reference probabilities and the newly acquired probabilities using fuzzy aggregation were compared, with the advantages of the fuzzy technique over other approaches explained. Lastly, the impact of applying the updated severe accident probabilities on containment integrity was quantitatively investigated in a Level 2 PSA model.

• International Journal of Computer Science & Network Security
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• 제23권8호
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• pp.199-203
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• 2023

Influential trend that widely reflected the software engineering industry is service oriented architecture. Vendors are migrating towards cloud environment to benefit their organization. Companies usually offer products and services with a goal to solve problems at customer end. Because customers are more interested in solution of their problem rather than focusing on products or services. In software industry the approach in which customers' problems are solved by providing services is known as software as a service. However, software development life cycle encounters enormous changes when migrating software from product model to service model. Enough research has been done on the overall development process but a limited work has been done on the factors that influence requirements elicitation process. This paper focuses on those changes that influence requirement elicitation process and proposes a systematic methodology for transformation of software from product to service model in a successful manner. The paper then elaborates the benefits that inherently come along with elicitation process in cloud environment. The paper also describes the problems during transformation. The paper concludes that requirement engineering process turn out to be more profitable after transformation of traditional software from product to service model.

• 한국정밀공학회지
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• 제32권10호
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• pp.845-850
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• 2015

This study was designed to investigate the feasibility of utilizing an adaptation for selective elicitation of tactile sensations by means of transcutaneous electrical stimulation. We conducted the first experiment to investigate how the stimulation frequency affected the adaptation. Twenty healthy subjects participated in the second experiment to confirm our proposal that the perception intensity of the low-frequency vibration can be enhanced after a high-frequency adaptation, and vice versa. It was found that (1) a low-frequency stimulation did not adapt the nerve afferents responsible for the high-frequency vibration, (2) a high-frequency stimulation affected the nerve afferents responsible for the low-frequency vibration, but adapted to the pressure sensation more intensely, and (3) more than 62% of the subjects reported a more clear selective sensation after the adaptation had lessened or depressed the unwanted sensation. The observations showed that adaptation of the nerve afferent could be utilized for selective elicitation of tactile sensations.

• 한국정보과학회논문지:시스템및이론
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• 제35권8호
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• pp.394-406
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• 2008

컨텍스트 인지 시스템에서 컨텍스트는 매우 중요한 요소이지만, 기존 요구공학은 컨텍스트를 도출하고 명세화하는 방법을 지원하지 못하기 때문에 이를 지원할 수 있는 연구가 필요하다. 본 논문은 컨텍스트 인지 시스템의 요구사항을 효과적으로 도출하기 위한 방법과 명세화 방법을 제안한다. 논문에서 제안하는 요구사항 도출 방법은 6단계로 구성된 점진적이고 반복적인 프로세스로서 비즈니스 로직을 위한 요구사항을 먼저 파악하고, 이를 기반으로 컨텍스트 로직을 위한 요구사항을 파악하고, 컨텍스트 모델링과 서브시스템 식별 등의 작업을 수행한다. 요구사항 명세화 방법으로는 컨텍스트 인지 유스케이스 다이어그램, 컨텍스트의 개념을 표현할 수 있는 컨텍스트 다이어그램, 컨텍스트 타입에 영향을 받는 유스케이스를 표현하는 컨텍스트타입-유스케이스 의존 다이어그램을 제안한다. 논문에서는 또한 제안한 방법을 적용한 시스템에 대한 사례연구를 소개하고, 이를 정성적으로 평가한 내용을 제시한다. 본 연구는 이해관계자가 컨텍스트 인지 시스템의 요구사항을 효과적으로 파악하고, 이를 명확히 기술하며, 이해할 수 있도록 도움을 줄 수 있다.

• 한국정보과학회논문지:소프트웨어및응용
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• 제33권8호
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• pp.655-667
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• 2006

본 논문은 목표 및 시나리오 기반의 요구사항을 이용하여 기능점수 (function point)를 산정하는 방법을 제안한다. 기능점수는 소프트웨어의 규모를 계산하는 방법으로 널리 사용되고 있으며 비용계산의 기본자료가 된다. 기능접수를 산정하기 위해서는 요구사항 도출 및 분석이 선행되어야 하나 기존의 기능점수 방법론은 이를 다루지 않고 있다. 한편 시스템 개발의 초기단계에서 대부분의 요구사항은 자연어 형태로 수집된다. 목표와 시나리오 방법론은 자연어 형태의 요구사항을 사용하여 요구사항을 도출하고 분석하는 방법으로 널리 사용되고 있으며 추적성에 대한 장점을 가지고 있다. 그러므로 목표 및 시나리오 기반의 요구사항으로부터 기능점수를 산정 할 수 있다면 요구사항과 기능접수 간의 추적성 관리가 쉬워진다. 이에 본 논문에서는 목표와 시나리오 기반의 요구사항으로부터 기능점수를 산정하는 방안을 제안한다. 제안된 방안은 자연어 형태로 기술된 목표 및 시나리오로부터 기능접수 분석에 필요한 규칙을 제공한다. 제안된 방안은 Order Processing System 예제를 통해 적용 방안을 설명한다.