• Journal of Microbiology and Biotechnology
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• v.10 no.1
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• pp.107-110
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• 2000

Elicited cells with methyl jasmonate continued to produce benzophenanthridine alkaloids throughout medium changes in suspension cultures of Eschscholtzia californica. Large increases in alkaloid production were observed by re-elitations with medium changes. The total alkaloid production increased during the successive elicitation steps reaching a maximum level on the 4th elicitation. The highest total alkaloid produced was 250 mg/I, which was 20fold higher than that of the single elicitation and 4-fold higher than that of the normal culture without elicitation. The large increases in alkaloid production in successive re-elicitations with medium changes are believed to be caused by the accumulation of the signal transduction compound, jasmonate.

• Journal of Sasang Constitutional Medicine
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• v.14 no.3
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• pp.114-131
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• 2002

1. PURPOSE : The purpose of this study is to investigate the effect of Chungpyesagantang on LPS induced Arthritis in Mice. 2. METHOD : All the BALB/C Mice used in this study were 4wks of age at the start of the experiment. The experimental model of Arthritis was induced by injectection of $300{\mu}g/kg$ LPS in mice knee joint. The experiment was compare daily CS treatment group after Arthritis elicitation with Arthritis elicitated group at day 4, 7, 14 after Arthritis elicitation. 3. RESULTS 1) The hyperplasia of synoviocytes of CS treatment group after Arthritis elicitation is soften than Arthritis elicitated group. 2) The aggregation of collagen fibers CS treatment group after Arthritis elicitation is decreased than Arthritis elicitated group. 3) The distribution of TUNEL positive cells(apoptotic cell) of CS treatment group was remarkably increased than Arthritis elicitated group. 4) The distribution of $TNF-{\alpha}$, $NF-{\kappa}B\;p50$, COX-2 positive cells of CS treatment group after Arthritis elicitation in synovial membrane was decreased than Arthritis elicitated group. 5) The distribution of $IL-2R-{\alpha}$, ICAM-1 positive cells of CS treatment group after Arthritis elicitation in apical surface of synovial membrane was decreased than Arthritis elicitated group. 6) The distribution of $NF-{\kappa}B\;p50$, $IL-2R-{\alpha}$ in common iliac lymph node of CS treatment group after Arthritis elicitation positive cells was decreased than Arthritis elicitated group. 4. CONCLUSION : As a result of these experimental results, it may be concluded that Chungpyesagantang used for treatment of LPS induced Arthritis in Mice. Inflamation activity in CS treatment group after Arthritis elicitation was decreased than Arthritis elicitated group.

• Journal of Microbiology and Biotechnology
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• v.9 no.3
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• pp.358-360
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• 1999

The effect of pectinase on menthol production by Mentha piperita in shake flasks was investigated. The optimum concentration of pectinase and period of elicitation for menthol production were 15 U/1 and 9 days, respectively. Pectinase elicitation at 15 U/1 for 9 days using a Lin-Staba medium with 2,4-dichlorophenoxyacetic acid (2,4-D) enhanced menthol production 37-fold (211.5 ㎎ menthol/l) with the specific menthol concentration (menthol concentration per unit weight of cells) of 27.5 ㎎/g dry cell weight (DCW). Our results also indicate that pectinase elicitation may activate the conversion of pulegone to menthol.

• Journal of Microbiology and Biotechnology
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• v.3 no.4
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• pp.238-243
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• 1993

In the suspension cultures of Eschscholtzia californica, phytohormone effects showed that alkaloid production was increased by IAA treatment without kinetin in both volumetric and specific way. Kinetin, however, suppressed alkaloid accumulation. Addition of ethephon inhibited cell growth. However, it enhanced the alkaloid production significantly in both volumetric and specific way. IAA promoted alkaloid production during elicitation. The highest alkaloid accumulation was observed at 5 $\mu$ M of IAA. Ethephon also enhanced alkaloid production during elicitation. The highest alkaloid formation was observed at 460 mg/l of ethephon with elicitation. Elicitation with ethephon, however, altered cell growth and the pattern of benzophenanthridine alkaloids production.

• Journal of the Korea Society of Computer and Information
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• v.24 no.3
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• pp.95-104
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• 2019

Crowdsourcing is growing its interests in designing where various designers work independently to a given design task. Recent researchers discovered that collaboration by sharing designs among designers helps to produce high-quality designs. However, design task may still be hard even with that collaboration in case the requirements are not well-defined. Most customers sometimes do not know what they really want and do not know how to clearly define the requirements. Consequently, the lack of requirements creates issues on designers, such as spending much time and effort on collecting requirements alone or from the customers. The designers even end up missing important necessities to complete their tasks. To address this issue, we proposed a collaborative requirements elicitation method that supports designers who are working on the same task. We developed CREFD (Collaborative Requirements Elicitation For Designers and Developers) tool to enable designers collaboratively provide requirements, identify dependencies, add annotations and votes to the provided requirements. We performed the hypothetical and empirical evaluations to test and compare the proposed method with one of the existing elicitation methods, the results show that the proposed method helps in collecting accepted and well-organized requirements better than individual requirements elicitation.

• Nuclear Engineering and Technology
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• v.53 no.10
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• pp.3298-3313
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• 2021

The objective of this study is to estimate the occurrence probabilities of severe accident phenomena based on a fuzzy elicitation technique. Normally, it is difficult to determine these probabilities due to the lack of information on severe accident progression and the highly uncertain values currently in use. In this case, fuzzy set theory (FST) can be best exploited. First, questions were devised for expert elicitation on technical issues of severe accident phenomena. To deal with ambiguities and the imprecision of previously developed (reference) probabilities, fuzzy aggregation methods based on FST were employed to derive the occurrence probabilities of severe accidents via four phases: 1) choosing experts, 2) quantifying weighting factors for the experts, 3) aggregating the experts' opinions, and 4) defuzzifying the fuzzy numbers. In this way, this study obtained expert elicitation results in the form of updated occurrence probabilities of severe accident phenomena in the OPR-1000 plant, after which the differences between the reference probabilities and the newly acquired probabilities using fuzzy aggregation were compared, with the advantages of the fuzzy technique over other approaches explained. Lastly, the impact of applying the updated severe accident probabilities on containment integrity was quantitatively investigated in a Level 2 PSA model.

• International Journal of Computer Science & Network Security
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• v.23 no.8
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• pp.199-203
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• 2023

Influential trend that widely reflected the software engineering industry is service oriented architecture. Vendors are migrating towards cloud environment to benefit their organization. Companies usually offer products and services with a goal to solve problems at customer end. Because customers are more interested in solution of their problem rather than focusing on products or services. In software industry the approach in which customers' problems are solved by providing services is known as software as a service. However, software development life cycle encounters enormous changes when migrating software from product model to service model. Enough research has been done on the overall development process but a limited work has been done on the factors that influence requirements elicitation process. This paper focuses on those changes that influence requirement elicitation process and proposes a systematic methodology for transformation of software from product to service model in a successful manner. The paper then elaborates the benefits that inherently come along with elicitation process in cloud environment. The paper also describes the problems during transformation. The paper concludes that requirement engineering process turn out to be more profitable after transformation of traditional software from product to service model.

• Journal of the Korean Society for Precision Engineering
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• v.32 no.10
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• pp.845-850
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• 2015

This study was designed to investigate the feasibility of utilizing an adaptation for selective elicitation of tactile sensations by means of transcutaneous electrical stimulation. We conducted the first experiment to investigate how the stimulation frequency affected the adaptation. Twenty healthy subjects participated in the second experiment to confirm our proposal that the perception intensity of the low-frequency vibration can be enhanced after a high-frequency adaptation, and vice versa. It was found that (1) a low-frequency stimulation did not adapt the nerve afferents responsible for the high-frequency vibration, (2) a high-frequency stimulation affected the nerve afferents responsible for the low-frequency vibration, but adapted to the pressure sensation more intensely, and (3) more than 62% of the subjects reported a more clear selective sensation after the adaptation had lessened or depressed the unwanted sensation. The observations showed that adaptation of the nerve afferent could be utilized for selective elicitation of tactile sensations.

• Journal of KIISE:Computer Systems and Theory
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• v.35 no.8
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• pp.394-406
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• 2008

Even though context is the most important feature in context-aware systems, the existing requirements engineering cannot support methodology for elicitation and specification of contexts. In this paper, we propose a requirements elicitation method and a requirements specification method for context-aware systems. Our requirements elicitation method is a 6-stepped, incremental, and iterative process. At the beginning steps in the process, we identify the requirements for business logic. Afterwards, we gather the requirements for context logic, model contexts, and identify subsystems. For requirements specification, we suggest a context-aware use case diagram, a context diagram for context modeling, and a context-type-use-case-dependency diagram for the traceability of use cases on the change of context types. We also introduce a case study that we apply our approaches to a real system, and a qualitative evaluation of our approaches. Our study will help stakeholders to efficiently elicit requirements for context-aware systems and to specify them clearly.

• Journal of KIISE:Software and Applications
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• v.33 no.8
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• pp.655-667
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• 2006

This paper proposes a method for counting function point using goal and scenario based requirements. Function Point is a software sizing method and widely used as a basis to estimate software development cost. Requirements elicitation and analysis should be performed before function point analysis but function point analysis method doesn't deal with requirements elicitation and analysis. For that reason, Function point extraction method from existing requirements method is needed and if the requirements method has advantage for traceability and elicitation, it is suitable for managing cost. Goal and scenario method is widely used as requirements elicitation and analysis. It has also good traceability. Therefore, this paper discusses a method for extracting function point from requirements text gathered using the goal and scenario based requirements elicitation technique. The proposed method aims to establish and maintain traceability between function point and requirements text. Text based function point extraction guidance rules have been developed. The proposed methodology has been applied to Order Processing System development.