• 제목/요약/키워드: Electric Field strength

검색결과 468건 처리시간 0.025초

Penicillin-G 첨가 배지에서 배양한 코리네형 세균의 전기장 충격법에 의한 고효율 형질전환 (High Frequency Electroporation-transformation of Coryneform Bacteria Grown in the Medium with Penicillin-G)

  • 노갑수;김성준
    • KSBB Journal
    • /
    • 제6권3호
    • /
    • pp.223-230
    • /
    • 1991
  • Using the shuttle vector pECCGl between Escherichia coli and Corynebacterium glutamicum and C. glutamicum strain JS231 grown in the medium supplemented with penicillin-G, which inhibits the formation of cross-links in the peptidoglycan of bacterial cell wall, various parameters involved in electroporation system including resistance, electric field strength, capacitance, DNA concentration, and cell density were investigated independently and optimized for the high efficiency transformation of coryneform bacteria. Using cells grown with 0.3U/ml of penicillin-G and harvested at A600 of 0.7-0.8, transformation efficiencies of 107-l08 transformants/$\mu\textrm{g}$ of DNA with Corynebcctertum glutamicum strain JS231 and wild type ATCC13032 were achieved under conditions of 12.5kV/cm of electric field strength, 400 ohms of resistance, $25\mu$F of capacitance, 3$\times$108 cells per transformation(1.2$\times$1010 cells/ml) and 100ng of plasmid DNA per transformation.

  • PDF

3 포트 ERF 밸브의 유량-방향 제어 성능 실험 -유압시스템 및 자동화 융합연구- (Performance Test of 3 Port ERF Valve for Controlling Flow Rate-Direction)

  • 장성철
    • 동력기계공학회지
    • /
    • 제16권6호
    • /
    • pp.92-97
    • /
    • 2012
  • In this study, ER fluids were working fluid and yield shear stress of attained dispersive ER fluids made by analyzed to adapt effectively at the practical equipment like three port ER valve. In addition, movement of ER particle was observed by using a microscope the same as the strength of electric field with Bingham characteristic. Basis material was presented to develop 3 port ER-Valve by quantitatively comparing and analyzing entrance, load and outlet flow's differences of 3 port rectangular tubes. This study, after designing and producing the 3port ER-Valve, considered the characteristic of pressure drop, outlet and load flow rate by changing the strength of electric field on ER fluids flowing between electrodes.

절연진단을 위한 기중방전에서의 방사전자파 측정과 분석 (The Measurement and Analysis The Electromagnetic Wave by Discharge in Air for Insulation Diagnosis)

  • 이상훈;이현동;이춘하;윤영대;김기채;이광식;이동인
    • 한국조명전기설비학회:학술대회논문집
    • /
    • 한국조명전기설비학회 1999년도 학술대회논문집-국제 전기방전 및 플라즈마 심포지엄 Proceedings of 1999 KIIEE Annual Conference-International Symposium of Electrical Discharge and Plasma
    • /
    • pp.141-144
    • /
    • 1999
  • This paper offer fundamental ones about insulation diagnosis with electromagnetic wave, when discharges occurred. To simulate Partial-Discharge(PD), It is used needle-plane electrode in air. As the voltages applied, measure the electromagnetic wave with monopole antenna and analysis its fundamental characteristics, then, acquired data were operated. The results presented as the form of graph, and the magnitude of charge when occurred PD simultaneously. In conclusion, when the magnitude of charge over 100[pC], the electric field strength changed, and then the magnitude of charge and the electric field strength increased samely.

  • PDF

$SF_6$ 가스중 PET Film의 절연특성 (Insulated Characteristics of PET Film under $SF_6$)

  • 김정달;정장근;주성철;이덕진;박재윤;이용길
    • 대한전기학회:학술대회논문집
    • /
    • 대한전기학회 1999년도 하계학술대회 논문집 E
    • /
    • pp.2120-2122
    • /
    • 1999
  • $SF_6$ gas has been widely used the gas insulated transmission line, gas insulated transformer and gas insulated substation and Polyester film used as the turn insulation with other material. $SF_6$ gas has been actively studied in domestic and foreign countries. In general, when dielectric surface electric field exceed any electric field strength on air, corona discharge is occurred from the dielectric surface. This paper presents the insulation strength of turn insulation of $SF_6$-PET film studied under application of AC voltage, constitute of the plane to plane with PET Film. The results obtained in experimental are summarized that Partial Discharge Initial Voltage(PDIV) of turn insulation depend on gas pressure, but Breakdown Voltage(BDV) did not depend on gas pressure.

  • PDF

22.9 KV-y 배전선로용 전선보호관 전계강도 비교실험 연구 (A study on the electric field strength of the insulation cover using 22.9 kV-y distribution line)

  • 전영갑;서길수;차기식
    • 대한전기학회:학술대회논문집
    • /
    • 대한전기학회 2000년도 하계학술대회 논문집 C
    • /
    • pp.1871-1873
    • /
    • 2000
  • There are many electrical accidents between bare wire and line post insulator which is degraded by long time using, causing leakage current on the surface of insulator. In this paper it is presented that the 22.9 Kv-y distribution lines are protected by binding cover which is needed from fog or rainy moisture, surges occurring by switching or lightening pulse. It is analyzed to investigate the unbalanced transfer characteristics and reflection coefficient by using binding cover and non-cover. It was tested the radio interference voltage in the test room and electric field strength by calculation between binding cover and non-cover. In the factory the leakage current causing on the surface of insulator was tested in the state of light load and heavy load.

  • PDF

광폭 전기집진기의 집진 특성에 관한 수치적 연구 (A Numerical Study on the Collection Characteristics of a Wide Plate-Spacing Electrostatic Precipitator)

  • 김용진;홍원석;정상현;하병길;하대홍;유주식
    • 설비공학논문집
    • /
    • 제13권12호
    • /
    • pp.1205-1213
    • /
    • 2001
  • The electrical characteristics and particle collection efficiency of a wide-plate spacing electrostatic precipitator are numerically investigated, and the results are compared with those obtained experimentally. The electric potential and field strength near the collection plate increase with increasing the plate spacing. The electric field strength of a discharge electrode of a twisted pin type is larger than that of a rectangular type. As the roughness factor of the discharge electrode wire becomes small, or the plate spacing becomes narrow, the corona current of the precipitator increases. The numerical results agree well with those obtained from experimental method.

  • PDF

$SF_6$ 가스차단기의 소전류 차단성능 해석 (Analysis of Small Current Interruption Performance for $SF_6$ Gas Circuit Breaker)

  • 김홍규;송기동;정진교;오연호;박경엽
    • 대한전기학회논문지:전기기기및에너지변환시스템부문B
    • /
    • 제55권10호
    • /
    • pp.528-533
    • /
    • 2006
  • To analyze the small current interruption performance for the gas circuit breakers, the gas density and electric field intensity should be calculated. In this paper, the FVFLIC method is used for the gas flow analysis and the FEM for the electric field analysis. Then, the dielectric withstanding voltage is evaluated by the empirical formulation or Streamer theory. By comparing the calculated dielectric strength with the test result, it is found that both methods show good prediction capability for the small current interruption performance. Especially, when both methods predict the same interrupting performance, the prediction is in accordance with the experimental result.

17.5 kV Recloser용 Bushing 2종에 대한 전계강도 연구 (A Study on the Electric Field Strength of Two Bushings for 17.5 kV Recloser)

  • 신영준;오연호;김민규;이정기;김수정;김진기;김귀식;정정훈
    • 대한전기학회:학술대회논문집
    • /
    • 대한전기학회 2003년도 추계학술대회 논문집 전력기술부문
    • /
    • pp.392-395
    • /
    • 2003
  • The study of electric field analyses has been conducted on two kinds of bushings for 17.5kV $SF_6$ gas insulated recloser. In this paper, two model bushings and input conditions for the analyses are described in detail. The results of the electric field analyses are presented and compared with to find out the better design in two bushings. Finally some suggestions are given for improving the insulation characteristics of the bushings.

  • PDF

8.2MHz 대역 미약 전계강도 무선기기의 출력기준에 관한 연구 (A Study on the Technical Regulation of Weak Electric Filed Strength Radio Equipment about 8.2Hz Frequency Band)

  • 김선엽;나유찬
    • 한국정보통신학회논문지
    • /
    • 제13권12호
    • /
    • pp.2498-2504
    • /
    • 2009
  • 본 논문에서는 8.2MHz 대역의 국내 미약 전계 강도 무선기기의 출력 제한치를 다른 외국의 기준치와 비교하였다. 이를 통해 국내의 규정이 미국이나 유럽에 비해 낮음을 대략 10-20dB 정도 낮음을 확인하였고, 이를 검증하기 위해 8.2MHz EAS 시스템의 출력을 측정하였다. 측정 결과, 전계 강도값이 각각 $70.6dB{\mu}V/m$$68.3dB{\mu}V/m$로 측정되었는데, 이러한 값은 국내의 현재기준인 $59.8dB{\mu}V/m$를 초과하는 값임을 확인하였다. 따라서 8.2MHz 대역의 국내기준의 사향 검토가 필요하다 사료된다.

58kHz 대역 미약 전계강도 무선기기 기술 기준에 관한 연구 (A Study on the Technical Regulation of Weak Electric Filed Strength Radio Equipment about 58kHz Frequency Band)

  • 박형근;김선엽;나유찬
    • 한국산학기술학회논문지
    • /
    • 제10권9호
    • /
    • pp.2319-2325
    • /
    • 2009
  • 본 논문에서는 58kHz 대역의 국내 미약 전계 강도 무선기기의 출력 제한치를 다른 외국의 기준치와 비교하였다. 이를 통해 국내의 규정이 미국이나 유럽에 비해 대략 50dB 정도 낮음을 확인하였고, 이를 검증하기 위해 58kHz EAS 시스템의 출력을 측정하였다. 측정 결과, 전계강도값이 각각 $112dB{\mu}V/m$$108dB{\mu}V/m$로 측정되었는데, 이러한 값은 국내의 현재 기준인 $102.7dB{\mu}V/m$를 초과하는 값임을 확인하였다. 따라서 58kHz 대역의 국내기준의 사양 검토가 필요하다고 사료된다.