• ALGAE
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• v.29 no.1
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• pp.47-55
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• 2014

To develop effective and safe acne vulgaris therapies with a continuing demand for new solutions, we investigated unique efficacy of an antibacterial agent from marine brown alga Eisenia bicyclis in treating acne vulgaris. The methanolic extract of E. bicyclis exhibited potential antibacterial activity against acne-related bacteria. The ethyl acetate fraction showed the strongest antibacterial activity against the bacteria among solvent fractions. Six compounds (1-6), previously isolated from the ethyl acetate fraction of E. bicyclis, were evaluated for antibacterial activity against acne-related bacteria. Among them, compound 2 (fucofuroeckol-A [FF]) exhibited the highest antibacterial activity against acne-related bacteria with a minimum inhibitory concentration (MIC) ranging from 32 to $128{\mu}g\;mL^{-1}$. Furthermore, FF clearly reversed the high-level erythromycin and lincomycin resistance of Propionibacterium acnes. The MIC values of erythromycin against P. acnes were dramatically reduced from 2,048 to $1.0{\mu}g\;mL^{-1}$ in combination with MIC of FF ($64{\mu}g\;mL^{-1}$). The fractional inhibitory concentration indices of erythromycin and lincomycin were measured from 0.500 to 0.751 in combination with 32 or $64{\mu}g\;mL^{-1}$ of FF against all tested P. acnes strains, suggesting that FF-erythromycin and FF-lincomycin combinations exert a weak synergistic effect against P. acnes. The results of this study suggest that the compounds derived from E. bicyclis can be a potential source of natural antibacterial agents and a pharmaceutical component against acnerelated bacteria.

• Journal of the Korea Organic Resources Recycling Association
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• v.21 no.1
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• pp.85-93
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• 2013

The amount of salted food waste and non-salted food waste from household consisted of 4 family members was surveyed. And the feeding rate and growth rate of Eisenia fetida population upon the different food wastes such as vegetables, grains, fruits and salted food waste were investigated. Total amount of food waste from a household was 3,200g/week. i.e. 0.114kg/day/capita and non-salted food waste was 53.9% of food waste. Salt contents of vegetables, grains, fruits were 0.60, 0.36 and 0.33%, respectively, if they were assumed to have 70% water content. Salt content of salted food waste was 0.78%. Upon the non-salted vegetables and fruits, feeding rate of earthworm was high but growth rate was low. Upon the non-salted grains, feeding rate of earthworm was low but growth rate was high. Upon the food with 70% water content, earthworm could not feed and grow when salt content of food was over 0.6%, and earthworm could feed normally but showed low growth rate when salt content of food was 0.3%. These results indicated that earthworm could stabilize the non-salted food wastes with ease but could hardly stabilize the salted food wastes.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.4
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• pp.274-279
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• 2007

The toxicitiy of organic sludge such as municipal sewage sludge (MSS), industrial sewage sludge (ISS), alcohol fermentation processing sludge (AFPS) and leather processing sludge (LPS) were evaluated with three environmental biomarkers as acetylcholinesterase, cytochrome P450, and heat shock protein 70 extracted from earthworm (Eisenia fetida). Their toxicities were compared with those of pig manure compost (PMC). MSS, ISS, LPS, and AFPS did not significantly affect the acetylcolinesterase activity, whereas only the elutriate of PMC slightly was increased the activity. MSS, AFPS, and PMC tended to slightly inhibit the cytochrome $P_{450}$ activity, but ISS and LPS showed significantly the inhibitory effect on cytochrome $P_{450}$. The hsp70 expression began to increase after treatments and showed high induction at 6 hour, followed by zero level at around 12 hour. The quantity of the hsp70 expressed by elutriate treatments of PMC, AFPS, MSS, ISS, and LPS was 1.9, 3.0, 3.3, 4.4, and 4.7 fold higher than that of distilled water. These results indicate that in toxicity tests of five organic waste materials, four kinds of sludge materials appeared more toxic than PMC. Results of AChE, P450, and hsp70 of earthworm might be useful for expecting or assessing an effect by exposure of organic wastes to earthworms in soil.

• Journal of the Korea Organic Resources Recycling Association
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• v.16 no.3
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• pp.52-64
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• 2008

Aged sewage sludge with food waste leachate sludge or paper mill sludge was fed to earthworm (Eisenia fetida) populations and the effect of it on feeding and growth rate of earthworm population was investigated. Feeding rate of earthworm population on sewage sludge aged with food waste leachate sludge for 30 days was higher than that aged for 10 and 20 days. At 90 days after feeding, the density of earthworm population decreased regardless of aging period. And earthworm population growth to next generation was blocked by sewage sludge. The feeding rate and the density of earthworm population aged with paper mill sludge for 20 days were higher than that on sewage sludges aged for 10 and 30 days. Until 90 days after feeding, the density of earthworm population increased regardless of aging period of sewage sludge, however, since then decreased. There were comparatively higher feeding rate and growth rate of earthworm population on the sewage sludge which had been mixed with paper mill sludge and aged for 20 days, which rendered the probable pretreating method for vermicomposting sewage sludge.

• Journal of the Korea Organic Resources Recycling Association
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• v.21 no.2
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• pp.79-87
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• 2013

Effects of pig manure with various composting periods(0, 15, 30, 50, 80 days) on feeding rate, biomass, cocoon production of earthworm(Eisenia fetida) population and physicochemical property of vermicast produced from pig manure were investigated. The feeding rate of earthworm was increased with longer composting period of pig manure. But the biomass production of earthworm population was highest upon the pig manure composted for 30 days. Upon the pig manure composted for 80 days, the biomass was severely reduced. Cocoon production was decreased with longer composting period and especially lower on the pig manure composted for 80 days. Values of pH, EC, C/N ratio of vermicasts produced from pig manure composts were lower than those of pig manures. And the organic material contents of vermicasts were uniformly reduced irrespective of composting duration of pig manure, whose values were 35.9-39.8%. From these results, the optimum composting period of pig manure for vermicomposting could be 15-30 days. And the application of vermicomposting upon the composted pig manure could be an efficient way for the treatment of pig feces, which can stabilize and recycle the organic wastes more rapidly than the conventional composting method.

• Journal of Nutrition and Health
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• v.41 no.6
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• pp.493-501
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• 2008

The objectives of this study were to investigate the antihyperglycemic and hypolipidmic effects of sea oak(Eisenia bicyclis, EB) in the diabetic state and to examine the appropriateness of formulated EB pill for the effects. The various test materials obtained from EB were included in the experimental diets with 15% fat/0.5% cholesterol and fed to streptozotocin-induced diabetic mice weighing $35.0{\pm}0.7$ g for three weeks but not in the control diet having the same composition. The test materials were EB dry powder, water and ethanol extracts, viscozyme-treated EB water extract(EB enzyme-TR) and formulated pill containing dry powders of the EB, two kinds of seaweed, black soybean, sesame, onion and garlic. BG was measured during feeding period and serum insulin, lipids and thiobarbituric acid reactive substances(TBARS) and intestinal disaccharidase activities were measured at the end of the three weeks of the feeding. BG increase was lower in the EB enzyme-TR group after 10 days of the experimental diet but lower in EB pill group after 15 days compared with the control group. Serum insulin levels were higher in the EB enzyme-TR and EB pill groups. Intestinal maltase but not sucrase activity was higher in EB enzyme-TR fed group than the control group. Serum levels of total cholesterol and triglyceride were reduced by the EB enzyme-TR and EB pill compared with the control diet. HDL-/total cholesterol was increased by all EB test materials. Serum TBARS levels were lower in the EB ethanol extract and EB pill groups than in the control group and tended to be lower in the other EB groups. It is concluded that the EB enzyme-TR is the best among the EB preparations to be utilized as a functional component for improving blood glucose and lipid profile in diabetic subjects in the future. However, the pill containing low level of the EB powder is also regarded as effective and readily usable when formulated with the several other ingredients of the proper composition.(Supported by the RIC Program of MOCIE, Korea).

• Korean Journal of Environmental Biology
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• v.29 no.1
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• pp.68-73
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• 2011

The single cell gel electrophoresis (SCGE) assay is a microelectrophoretic technique for assessments of DNA damage at the level of the individual eukaryotic cell. The SCGE assay, due to its simplicity, sensitivity and need of a few cells, has advantages compared to other genomic damage assays such as sister chromatid exchange, chromosomal aberration and micronucleus test. In this study, investigated were the levels of DNA damage and the repair kinetics in the coelomocytes of Eisenia fetida treated with HgCl2 and ionizing radiation by means of the SCGE assay. For detecting DNA damage and repair in coelomocytes, earthworms (E. fetida) were irradiated with six doses of ${\gamma}$-rays (0, 2.5, 5, 10, 20 and 50 Gy) and in vivo exposed to mercuric chloride at 0, 80 and 160 mg $kg^{-1}$ for 48 hours. Then the Olive tail moments were measured during 0~12 hours after irradiation and 0~72 hours after Hg treatment. The results showed that the more the oxidative stress was induced by mercury and radiation, the longer the repair time was required. Also, the results suggest that the SCGE assay may be used as an important tool for comparison of the sensitivity of different species to oxidative stresses.

• Korean Journal of Environment and Ecology
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• v.28 no.6
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• pp.613-626
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• 2014

Seaweeds were identified after qualitative sampling at 14 stations of Dokdo coasts from May to July 2013 and seaweeds and animals inhabiting 23 Eisenia bicyclis plants were examined to evaluate its ecological role. Biomass was calculated by using regression between stipe length and weight of E. bicyclis. A total of 128 species were identified, including 18 green, 35 brown, and 75 red algae. Coarsely branched form was dominant functional group occupying 47.66% and 91 species (71.09%) were in ESG I group, growing in stable environmental coast. Also, R/P, C/P, and (R+C)/P were 2.14, 0.51, and 2.66, respectively showing temperate and mixed flora. Biomass and density of E.bicyclis were $23.74kg\;m^{-2}$ and 64 fronds $m^{-2}$. Twelve seaweeds and 83 animal species (15 Annelida, 25 Mollusca, 34 Arthropoda, 3 Echinodermata, and 6 others) were observed from 23 holdfasts and Ericthonius pugnax was dominant taxon having 538 (43.11%) of 1,248 animal individuals. These results indicate that E.bicyclis is a keystone species showing very important ecological role. In conclusion, the number of seaweeds increased because of intensive research and dominance of coarsely branched form and ESG I group seaweeds, representing that environmental condition of Dokdo is still intact.

• The Korean Journal of Soil Zoology
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• v.7 no.1_2
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• pp.17-21
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• 2002

Food waste which had been mixed with night soil sludge and composted for 21 days (mixing ratio = food waste/paper mill sludge : 1, w/w) was supplied to the 5 g of Eisenia fetida popualtion at various temperatures (5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 31, 32, 33, 34, 35$^{\circ}C$). The biomass of earthworms one month after feeding at 20-32$^{\circ}C$ was about two times more than that of the introduced earthworms, increasing rate of which was much higher than them at the other tested temperatures . There were decreases at temperatures below 7$^{\circ}C$, and less increases at temperatures of 8-10$^{\circ}C$ in biomass. Earthworms could not survive at temperatures at 34$^{\circ}C$ and 35$^{\circ}C$. Feeding rates of earthworms were much higher at 20-32$^{\circ}C$ than them at the other tested temperatures, and there were no differences among them at temperatures of 20-32$^{\circ}C$. But at 3 months after feeding, the biomass and feeding rate of earthworms decreased severely even at 20$^{\circ}C$ and 30$^{\circ}C$, which was supposed to be due to the long term effect of salts, especially sodium chloride (NaCl) in the food wastes.

• Proceedings of the Korean Society of Soil Zoology Conference
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• 2001.10a
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• pp.20-20
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• 2001

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