• Title/Summary/Keyword: Eisenia

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Estimation of optimal phosphorus and calcium levels on the growth and cast production of earthworm(Eisenia foetida) (지렁이의 생육과 분립생산을 위한 적정 인과 칼슘 수준의 추정)

  • Lee, Ju-Sam;Lee, Pil-Won
    • Journal of the Korea Organic Resources Recycling Association
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    • v.10 no.4
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    • pp.96-102
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    • 2002
  • The purpose of this study was estimate the optimal phosphorus and calcium levels of Korean native cow manure on the growth and cast production of earthworm(Eisenia foetida). Phosphorus and calcium levels supplemented to cow manure were 0%(without earthworm), 2% and 4%, and 0.01%, 0.02%, 0.04% and 0.06% of the cow manure by weight, respectively. The maximum fresh weight and increasing rate of earthworm were obtained with 2% phosphorus level, but there was not significant differences between calcium levels. The ratios of available phosphorus to calcium contents of cast were 1.04-1.20:1 with 2%, and 1.28-1.37:1 with 4% phosphorus levels. Conversion rate of organic matter to earthworm tissue were 0.35-0.41% with 2%, and 0.25-0.40% with 4% phosphorus levels.

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Contrasting Correlation in the Inhibition Response of ADP-induced Platelet Aggregation and the Anti-coagulant Activities of Algal fucoidans Derived from Eisenia bicyclis and Undaria pinnatifida sporophylls (Mekabu)

  • Jeong, Eui-Sook;Yoon, Yeon-Hee;Kim, Jong-Ki
    • Fisheries and Aquatic Sciences
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    • v.12 no.3
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    • pp.194-202
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    • 2009
  • Sulfated fucans are known to have both anti-thrombotic and anti-coagulant activities. In this study, the variation in platelet aggregation and anti-coagulant activities was investigated in vitro with regard to administered dose, molecular weight distribution, sulfate content, and sugar composition in two algal fucoidans from Eisenia bicyclis and Undaria pinnatifida sporophylls (Mekabu). The anti-coagulant activity largely correlated with sulfate content and with molecular weight distribution in a dose-dependent manner. However, both fucoidans demonstrated inhibitory responses to ADP-induced platelet aggregation in dose- and structure-dependent manners that contrasted with the anti-coagulant activity. Neither molecular weight distribution nor sulfate content greatly affected platelet-aggregation inhibition (PA-inhibition) by the fucoidan fractions, whereas anti-coagulant activity was sensitive to these structural factors. Interestingly, an E. bicyclis fucoidan fraction exhibited almost complete PA-inhibition at a treatment dose of 500 mg/mL while retaining weak anti-coagulant activity. In conclusion, these observations suggest that fucoidan may be a useful anti-thrombotic or anti-platelet agent in various arterial thrombotic disorders, including post-vascular intervention with controlled bleeding complications, due to its anti-coagulant modulating activity.

Analysis of Reproduction and CO I Gene Sequence between Two Earthworm Populations Used in Vermicomposting Organic Wastes in Different Localities (서로 다른 지역에서 유기성 폐기물 처리에 이용되는 두 지렁이 집단의 생식 및 CO I 유전자 분석)

  • Bae, Yoon-Hwan
    • Journal of the Korea Organic Resources Recycling Association
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    • v.26 no.3
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    • pp.39-46
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    • 2018
  • Two domestic earthworm populations used in vermicomposting in different lacalities were collected. The one was from Hongcheon (Kangwon province) and the other was from Youngdong (Chungcheong province). Reproductive capacities and the degree of reproductive isolation of two population were investigated. CO I gene sequences were also compared. There was no difference in their reproductive capacities. And there was no reproductive isolation between two populations. Two populations were identified as Eisenia andrei or Eisenia fetida by CO I gene biomarkers. Phylogenetic tree formulated by CO I gene sequence strongly suggested that two populations were just the same species.

New Record of Axiothella quadrimaculata Augener, 1914 (Polychaeta : Maldanidae) in Korea (한국미기록 사방연통대 나무갯지렁이 Axiothella quadrimaculata Augener, 1914)

  • YUN Sung Gyn;PAIK Sang-Gyu;PAIK Eui-In
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.1
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    • pp.1-4
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    • 1999
  • A maldanid polychaete, Axiothella quadrimaculata Augener, 1914, is newly recorded in Korean fauna. Genus Axiothella is found for the first time in Korean waters. The specimen was collected in the Eisenia bicyclis bed by SCUBA diving. The species is described with figures and the key to genera was revised. Including this Axiothella quadrimaculata, a total of 18 species representing 13 genera are known to the Korean Maldanid polychaetes.

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Effect of supplementing phosphorus to Korean cow manure on the growth and cast production of earthworm (Eisenia foetida) (한우분내에 인의 첨가가 지렁이의 생육과 분립생산량에 미치는 영향)

  • 이주삼;이필원
    • Journal of Animal Environmental Science
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    • v.7 no.2
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    • pp.127-136
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    • 2001
  • This experiment was carried out to investigate the effect of supplementing phosphorus to Korean cow manure on the growth and cast production of earthworm(Eisenia Foetida), and to estimate the optimal phosphorus level for the growth and cast production of earthworm and ratios of available phosphorus and calcium content of cast in optimal phosphorus levels. Phosphorus supplementing ratios to Korean native cow were 0, 1%, 2%, 4% and 8A%, respectively. The volume of raising box was 3375cm$^3$(15$\times$15$\times$15cm), and 500g of cow manure filled up to 10cm layer. Raising density was 90㎤ per worm during the experimental period(60 days). The maximum fresh weight and cast production of earthworm were obtained at 2~4% and 1% levels of phosphorus supplementation to Korea native cow manure. The total phosphorus and available phosphorus contents of earthworm tissues were not significant among in all treatments. There was a positive significantly differences between total phosphorus and available phosphorus of cast and residual matters. Available phosphorus content of cast were ranged from 9.3mg/g to 17.3mg/g at 1~4% levels of phosphorus supplementation. Ratios of available phosphorus and calcium contents of cast were 1.94~3.15:1 and 0.87~1.33:1 at 2~4% and 0~1% levels of phosphorus supplementation.

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A novel BACE inhibitor isolated from Eisenia bicyclis exhibits neuroprotective activity against β-amyloid toxicity

  • Lee, Jung Kwon;Byun, Hee-Guk
    • Fisheries and Aquatic Sciences
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    • v.21 no.12
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    • pp.38.1-38.9
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    • 2018
  • Alzheimer's disease (AD) is a disturbing and advanced neurodegenerative disease and is characterized pathologically by the accumulation of amyloid beta ($A{\beta}$) and the hyperphosphorylation of tau proteins in the brain. The deposition of $A{\beta}$ aggregates triggers synaptic dysfunction, and neurodegeneration, which lead to cognitive disorders. Here, we found that FF isolated from an eatable perennial brown seaweed E.bicyclis protect against $A{\beta}$-induced neurotoxicity in neuroblastoma cells stably transfected with two amyloid precursor protein (APP) constructs: the APP695 cDNA (SH-SY5Y-APP695swe). The FF demonstrated strong inhibitory activity for ${\beta}$-secretase ($IC_{50}$ $16.1{\mu}M$) and its inhibition pattern was investigated using Lineweaver-Burk and Dixon plots, and found to be non-competitive. Then, we tested whether FF could inhibit production of $A{\beta}$ in SH-SY5Y-APP695swe. FF inhibited the production of $A{\beta}$ and soluble-APP, residue of APP from cleaved APP by ${\beta}$-secretase. Our data show that FF can inhibit the production of $A{\beta}$ and soluble-$APP{\beta}$ via inhibition of ${\beta}$-secretase activity. Taken together these results suggest that FF may be worthy of future study as an anti-AD treatment.

Acute Toxicity on Eisenia fetida of Six Major Chemicals Accidentally Spilled into the Environment (주요 사고 화학물질 6종의 줄지렁이에 대한 급성독성)

  • Nam, Tae-Hoon;Yun, Jeonghyeon;Choi, Jaeil;Lee, Sung-Eun
    • Korean Journal of Environmental Biology
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    • v.35 no.2
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    • pp.123-127
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    • 2017
  • To determine their acute toxicities on the earthworm (Eisenia fetida), six toxic chemicals were evaluated, according to the OECD guideline 207: sulfuric acid, methanol, methylethylketone, nitric acid, formic acid, and toluene. Sulfuric acid exhibited the maximum toxicity. The $LC_{50}$ values of sulfuric acid, nitric acid, formic acid, and toluene were 20.5, 49.1, 55.5, and $534.5{\mu}g\;cm^{-2}$, respectively. Toluene showed 26-fold lower toxicity than sulfuric acid. In this study, methanol and methylethylketone did not exhibit any toxicity to the earthworm. Further evaluation revealed that nitric acid, formic acid, and toluene exerted a change in the body weight of the chemically treated earthworms, whereas the other chemicals were ineffective. These results can be used for environmental risk assessment, when the chemicals are accidently discharged into the environment.