Journal of the Korea Organic Resources Recycling Association (유기물자원화)

Korea Organic Resources Recycling Association (유기성자원학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of Reproduction and CO I Gene Sequence between Two Earthworm Populations Used in Vermicomposting Organic Wastes in Different Localities

서로 다른 지역에서 유기성 폐기물 처리에 이용되는 두 지렁이 집단의 생식 및 CO I 유전자 분석

  • Bae, Yoon-Hwan (Dept. of Life Science and Chemistry, Daejin University)
  • 배윤환 (대진대학교 생명화학부)
  • Received : 2018.09.03
  • Accepted : 2018.09.15
  • Published : 2018.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Two domestic earthworm populations used in vermicomposting in different lacalities were collected. The one was from Hongcheon (Kangwon province) and the other was from Youngdong (Chungcheong province). Reproductive capacities and the degree of reproductive isolation of two population were investigated. CO I gene sequences were also compared. There was no difference in their reproductive capacities. And there was no reproductive isolation between two populations. Two populations were identified as Eisenia andrei or Eisenia fetida by CO I gene biomarkers. Phylogenetic tree formulated by CO I gene sequence strongly suggested that two populations were just the same species.

유기성 폐기물 처리를 위해 강원도 홍천과 충청도 영동에서 사육되고 있는 두 지렁이 집단의 생식력 차이, 생식적 격리 정도 및 CO I 유전자의 서열을 비교하였다. 두 집단의 생식력에 큰 차이가 없었으며, 두 집단 간에 생식적 격리도 전혀 이루어지지 않았다. CO I 유전자 염기 서열을 분석하여 두 집단의 종을 동정한 결과 붉은줄지렁이 또는 줄지렁이로 확인되었고, 두 집단의 유연관계가 매우 가까운 것으로 나타났다. 따라서 두 집단은 같은 지렁이 종임을 강력히 시사하였다.

Keywords

References

  1. Choi, H. G., "A study on sludge feed and breeding condition in vermicomposting of organic sludge", Ph. D. thesis, p. 114, Dept. of Environmental Engineering, Univ. of Seoul City. (1992).
  2. Edwards, C. A. and Bohlen, P. J., "Biology and Ecology of earthworm", Chapman and Hall, p. 426. (1996).
  3. Garg, P., Gupta, A. and Satya, S., "Vermicomposting of different types of waste using Eisenia foetida: A comparative study", Bior. Technol., 97, pp. 391-395. (2006). https://doi.org/10.1016/j.biortech.2005.03.009
  4. Park, K. I. and Bae, Y. H., "Feeding efficiency and growth rates of tiger worms (Eisenia fetida Savigny) when they were fed with differently pretreated sewage sludge", J. of the Korea organic waste recycling council, 11(4), pp. 66-78. (2003).
  5. Kaushik, P. and Garg, V. K., "Vermicomposting of mixed solid textile mill sludge and cow dung with the epigeic earthworm Eisenia foetida.", Biores. Technol., 90, pp. 311-316. (2003). https://doi.org/10.1016/S0960-8524(03)00146-9
  6. Gupta, R. and Garg, V. K., "Stabilization of primary sewage sludge during vermicomposting", J. of Hazardous Materials, 153, pp. 1023-1030. (2008). https://doi.org/10.1016/j.jhazmat.2007.09.055
  7. Decaens T., Porco D., Rougerie R., Brown G. G. and James S. W., "Potential of DNA barcoding for earthworm research in taxonomy and ecology", Applied Soil Ecology, 65, pp. 35-42. (2013). https://doi.org/10.1016/j.apsoil.2013.01.001
  8. Brown, W. M., George, J. R. M. and Wilson, A. C., "Rapid evolution of animal mitichondrial DNA", Natl. Acad. Sci. USA., 76, pp. 95-130. (1979).
  9. Birky, C. W., Furest, P. Jr. and Maruyama, T., "Organelle gene diversity under migration, mutation and drift: equilibrium expectations, approach to equilibrium, effects of heteroplastic cells, and comparison to nuclear genes. Genetics, 121, pp. 613-627. (1989).
  10. Moritz, C., Dowling, T. E. and Brown, W. A., "Evolution of animal mitochondria : relevance for population biology and systematics", Annu. Rev. Ecol. Syst., 187, pp. 269-292. (1987).
  11. Kim, H. K., Lee, M. Y., Lee, M. L., Choi, Y. S., Hong, I. P., Kim, N. S., Lee, K. G. and Jin, B. R., "Genetic diversity of the Apis cerana in Korea by mitochondrial DNA and ITS ribosomal DNA sequence", Korean J. Apiculture, 25(1), pp. 9-16. (2010).
  12. Hong, Y., James, S. W., Hwang, U. W., Lee, B. E., Park, S. C. and Kim, T. H., "Molecular phylogeny of the Amynthas-complex (Oligochaeta: Megascolecidae) inferred from ITS nucleotide sequences", Korean J. Environ. Biol., 25(4), pp. 349-355. (2007).
  13. Reineke, A. J. and Viljoen, S. A., "A comparoson of the biology of Eisenia fetida and Eisenia andrei (Oligochaeta). Biol. Fertil. Soils, 11, pp. 295-300. (1991). https://doi.org/10.1007/BF00335851
  14. Roch, P., Valembois, P. and Lassegues, M., "Biochemical particulars of the antibacterial factor of the two subspecies Eisenia fetida and Eisenia fetida andrei. Am. Zool., 20, pp. 790-794. (1980).
  15. Sims, R. W. and Gerard, B. M., "Earthworms. In: Kermac, D. M., Barnes, R. S. K. (EDs.)., Synopses of the British Fauna (New Series), N0. 31. Published for the Linnean Society of London and the Estuarine and Brackish-water Science Association, London". (1985).
  16. Dominguez J., Velando, A. and Ferreiro, A., "Are Eisenia fetida (Savigny) and Eisenia andrei (1972) (Oligochaeta, Lumbricidae) different biological species?", Pedo biologia, 49, pp. 81-87. (2005). https://doi.org/10.1007/s00000-005-1084-3
  17. Bradley, R. D. and Baker, R. J., "A test of genetic species concept: cytochrome b sequences and mammals", J. Mammalogy, 82, pp. 960-973. (2001). https://doi.org/10.1644/1545-1542(2001)082<0960:ATOTGS>2.0.CO;2