• Korean Journal of Environmental Agriculture
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• v.24 no.3
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• pp.289-294
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• 2005

The toxic effects of 12 commercially available insecticides on the earthworm, Eisenia fetida (Savigny), were evaluated using artificial soil, surface sprayed soil, immersion, and contact filter tests. The risk to earthworm was assessed by the TER (toxic exposure ratio) value, which was calculated by the formula, TER=NOEC/PEC (predicted environmental concentration). TER was 3 for methomyl SL, 20 for carbaryl WP, 20 for phosphamidon SL, 30 for imidacloprid WP, and 60 for dichlorvos EC the in artificial soil test. At recommended, the earthworm mortality to methomyl SL reached 50% in the surface sprayed soil test, 72% in the immersion test, 30% in the contact filter paper test, whereas that to imidacloprid WP reached 56, 32, and 100 respectively. As a result of the four methods, methomyl SL and imidacloprid WP would be toxic insecticides to earthworm.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.5
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• pp.280-284
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• 2006

The toxic effects of 14 commercially available fungicides on the earthworm, Eisenia fetida (Savigny), were evaluated using surface sprayed soil, immersion and contact filter tests. At recommended dose, the earthworm mortality is 4% on mancozeb, carbendazim, propineb and captan in the surface sprayed soil test, 4% on mancozeb, 6% on propineb and 10% on captan in the immersion test, and no effect on all used chemicals in contact filter test.

• Fisheries and Aquatic Sciences
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• v.21 no.11
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• pp.35.1-35.7
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• 2018

Background: The aim of this study was to investigate the in vitro inhibitory effects of Fucofuroeckol-A isolated from Eisenia bicyclis against tyrosinase activity and 3-isobutyl-1-methylxanthine (IBMX)-induced melanin biosynthesis in B16F10 melanoma cells. Result: Among the ethanolic (EtOH) extract of E. bicyclis and its organic solvent fractions, the ethyl acetate (EtOAc) soluble fraction showed a noticeable inhibitory effect on mushroom tyrosinase with an $IC_{50}$ value of $37.6{\pm}0.1{\mu}g/mL$. Repeated column chromatography of the active EtOAc fraction resulted in the isolation of Fucofuroeckol-A. It evidenced more potent tyrosinase inhibitory effect with an $IC_{50}$ value of $11.4{\pm}1.4{\mu}M$ than arbutin ($IC_{50}=1076.6{\pm}44.3{\mu}M$), which was used as a positive control. Lineweaver-Burk plots suggest that Fucofuroeckol-A plays as a noncompetitive inhibitor against tyrosinase. Furthermore, we have evaluated the inhibitory effects of Fucofuroeckol-A on IBMX-induced melanin formation in B16F10 melanoma cells. Fucofuroeckol-A ($12.5-100{\mu}M$) exhibited a significant inhibition of melanin production in the melanoma cells. Conclusion: In the present study, we suggested that Fucofuroeckol-A might prove possibility as a novel inhibitor of melanin biosynthesis in cosmetic applications.

• ALGAE
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• v.37 no.1
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• pp.63-74
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• 2022

Perennial stipitate kelps are globally distributed and individual species can inhabit broad latitudinal ranges, expressing notably longevous persistence. Despite the foundational role kelps provide to their communities, little is known about the variability in persistence of the stipitate kelps at local spatial scales. We studied the population persistence of Eisenia arborea, a heat- and wave force-tolerant perennial stipitate kelp with a distributional range extending from British Columbia to south of the range limit of all other northeast Pacific kelps, in Baja California Sur, Mexico. Persistence characteristics for E. arborea among sites were compared and used to test the hypothesis that stand persistence varied at local spatial scales around Isla Natividad, a Pacific island off the Baja California peninsula with documented spatiotemporal environmental heterogeneity. Collected individuals around the island were "aged" using the previously validated age estimation technique of counting annual cortical dark rings. After detecting no significant differences among sites in the covariation between estimated ages for collected individuals and stipe length, we utilized in-situ population-level stipe length measurements to more rapidly predict age structures within six stands around the island. Predicted age structures, and associated stand densities, revealed persistence characteristics and density varied at local scales and a strong positive relationship existed between stand density and stand mean and maximum ages. We speculate that stands responded differently to deterministic influences (e.g., the 2014-2016 marine heatwave and / or competition with Macrocystis) resulting in heterogenous local persistence of this foundation species.

• Journal of the Korea Organic Resources Recycling Association
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• v.18 no.3
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• pp.31-37
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• 2010

Chitinases (EC 3.2.1.14) hydrolyze the ${\beta}$-1,4-linkages in chitin, the second most abundant polymer of N-acetyl-${\beta}$-D-glucosamine which is a structural component of protective biological matrices such as fungal cell walls and insect exoskeletons. The glycosyl hydrolases 18 family including chitinases is an ancient gene family widely expressed in archea, prokaryotes and eukaryotes. Since earthworms live in the soil with a lot of microbial activities and fungi are supposed to be a major component of the diet of earthworm, it has been reported that there would be appropriate immune system to protect themselves from microorganisms attacks. In this study, the novel chitinase, EaChi, from the midgut of earthworm, Eisenia andrei, were identified and characterized. To obtain full-length cDNA sequence of chitinase, RT-PCR and RACE-PCR analyses were carried out by using the previously identified EST sequence amongst cDNA library established from the midgut of E. andrei. EaChi, a partial chitinase gene, was composed of 927 nucleotides encoding 309 amino acids. By the multiple sequence alignments of amino acids with other different species, it was revealed that EaCHI is a member of glycosyl hydrolases 18 family, which has two highly conserved domains, substrate binding and catalytic domain.

• Journal of the Korea Organic Resources Recycling Association
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• v.12 no.4
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• pp.105-111
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• 2004

When the clitellate tiger worms(Eisenia fetida) were fed with paper mill sludge, sewage sludge of agricultural-urban area and sewage sludge of agricultural area, number of cocoons produced by individual clitellate earthworm for 104 days were 11.8, 8.6 and 3.5, respectively. However, clitellate earthworms that had been fed with sewage sludge of agricultural-urban area for 52 days or fed with sewage sludge of agricultural area for 95 days could not produce cocoons, whereas the earthworms that were fed with paper mill sludge kept producing cocoons through the investigating period. The hatchability of cocoons produced by the clitellate tiger worms(Eisenia fetida) fed with sewage sludge of agricultural-urban region, sewage sludge of agricultural region and paper mill sludge were 42.8%, 38.3% and 39.2%, respectively. And hatching periods of cocoons were 33.8~36.0 days, which were not affected by the kinds of sludges. However, the cocoons produced by clitellate earthworms which had been fed with sewage sludge for about 1.5 months could not hatch at all. Death rates of second generation hatched from cocoons produced by tiger worms(Eisenia fetida) which had been fed with the sewage sludge from agricultural-urban region and the sewage sludge from agricultural region reached to 100% and 90% at 60 days after hatching, respectively, whereas death rate of second generation fed with paper mill sludge reached to 16%.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.550-558
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• 2021

In a radiotracer study, the bioaccumulation and efflux of metals in earthworms (Eisenia fetida) exposed to soil spiked with ZnO and Ag nanoparticles (AgNP) were compared to those exposed to soil spiked with ionic Zn and Ag. Additionally, the bioavailability and chemical mobility of nano- and ionic metals in the soil were estimated using the sequential extraction method and compared to the bioaccumulation factor(BAF). The BAF for ZnO (0.06) was 31 times lower than that for Zn ions (1.86), suggesting that ZnO was less bioavailable than the ionic form in contaminated soil. In contrast, the BAFs for two types of AgNPs coated with polyvinylpyrrolidone (0.12) or citrate (0.11) were comparable to those of ionic Ag (0.17). The sequential extraction of metals from the soil suggests that the chemically mobile fractions in the Zn ion treatment were higher(35%) than those (<20%) in the Ag ion treatment, which was consistent with the greater BAFs in the former than the latter. However, the chemical mobility in the ZnO treatments did not predict bioavailability. The efflux rates of Ag (3.2-3.8% d^-1) in the worms were 2-3×those(1.2-1.7% d^-1) for Zn.

• Proceedings of the Korean Society of Soil Zoology Conference
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• 2003.06a
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• pp.17-17
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• 2003

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.05a
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• pp.12-12
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• 2002

• 대한방사선방어학회:학술대회논문집
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• 2011.04a
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• pp.10-11
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• 2011