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http://dx.doi.org/10.11626/KJEB.2021.39.4.550

Bioaccumulation of Ag and Zn in earthworms (Eisenia fetida) from soil contaminated with Ag and Zn nanoparticles using a radiotracer method  

Seung Ha Lee (Department of Oceanography, Chonnam National University)
Byeong-Gweon Lee (Department of Oceanography, Chonnam National University)
Publication Information
Korean Journal of Environmental Biology / v.39, no.4, 2021 , pp. 550-558 More about this Journal
Abstract
In a radiotracer study, the bioaccumulation and efflux of metals in earthworms (Eisenia fetida) exposed to soil spiked with ZnO and Ag nanoparticles (AgNP) were compared to those exposed to soil spiked with ionic Zn and Ag. Additionally, the bioavailability and chemical mobility of nano- and ionic metals in the soil were estimated using the sequential extraction method and compared to the bioaccumulation factor(BAF). The BAF for ZnO (0.06) was 31 times lower than that for Zn ions (1.86), suggesting that ZnO was less bioavailable than the ionic form in contaminated soil. In contrast, the BAFs for two types of AgNPs coated with polyvinylpyrrolidone (0.12) or citrate (0.11) were comparable to those of ionic Ag (0.17). The sequential extraction of metals from the soil suggests that the chemically mobile fractions in the Zn ion treatment were higher(35%) than those (<20%) in the Ag ion treatment, which was consistent with the greater BAFs in the former than the latter. However, the chemical mobility in the ZnO treatments did not predict bioavailability. The efflux rates of Ag (3.2-3.8% d-1) in the worms were 2-3×those(1.2-1.7% d-1) for Zn.
Keywords
earthworm(Eisenia fetida); metal bioaccumulation; nanoparticle; radiotracer method; radiotracer;
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1 Adeel M, N Shakoor, M Shafiq, A Pavlicek, F Part, C Zafiu, A Raza, MA Ahmad, G Jilani, JC White, E-K Ehmoser, I Lynch, X Ming and Y Rui. 2021. A critical review of the environmental impacts of manufactured nano-objects on earthworm species. Environ. Pollut. 290:118041.
2 Alloway BJ. 2008. Zinc in the Soil and Crop Nutrition. 2nd Edition. International Zinc Association. Durham, NC. p. 139.
3 Arshi N, F Ahmed, S Kumar, MS Anwar, BH Koo and CG Lee. 2010. Comparative study of the Ag/PVP nano composites synthesized in water and in ethylene glycol. Curr. Appl. Phys. 11:S346-S349.
4 Basta N and R Gradwohl. 2000. Estimation of Cd, Pb, and Zn bioavailability in smelter-contaminated soils by a sequential extraction procedure. J. Soil Contam. 9:149-164.   DOI
5 Chinnapongse SL, RI MacCuspie and VA Hackley. 2011. Persistence of singly dispersed silver nanoparticles in natural freshwaters, synthetic seawater, and simulated estuarine waters. Sci. Total Environ. 409:2443-2450.   DOI
6 Clark SB, WM Johnson, MA Malek, SM Serkiz and TG Hinton. 1996. A comparison of sequential extraction techniques to estimate geochemical controls on the mobility of fission product, actinide, and heavy metal contamination in soils. Radiochim. Acta 74:131-179.
7 Cosin DJD, MP Ruiz and MH Garvin. 2002. Gut load and transit time in Hormogaster elisae (Oligochaeta, Hormogastridae) in laboratory cultures. Eur. J. Soil Biol. 38:43-46.   DOI
8 Croteau MN, SN Luoma and B Pellet. 2007. Determining metal assimilation efficiency in aquatic invertebrates using enriched stable metal isotope tracers. Aquat. Toxicol. 83:116-125.   DOI
9 Dybowska AD, MN Croteau, SK Misra, D Berhanu, SN Luoma, P Christian, P O'Brien and E Valsami-Jones. 2011. Synthesis of isotopically modified ZnO nanoparticles and their potential as nanotoxicity tracers. Environ. Pollut. 159:266-273.   DOI
10 Gottschalk F, T Sonderer, RW Scholz and B Nowack. 2009. Modeled environmental concentrations of engineered nanomaterials (TiO2, ZnO, Ag, Fullerenes) for different regions. Environ. Sci. Technol. 43:9216-9222.   DOI
11 He E, H Qiu, X Huang, CAM van Gestel and R Qiu. 2019. Different dynamic accumulation and toxicity of ZnO nanoparticles and ionic Zn in the soil sentinel organism Enchytraeus crypticus. Envrion. Pollut. 245:510-518.   DOI
12 Hou HC, P Westerhoff and JD Posner. 2013. Biological accumulation of engineered nanomaterials: a review of current knowledge. Environ. Sci. - Processes Impacts 15:103-122.   DOI
13 Hu CW, M Li, YB Cui, DS Li, J Chen and LY Yang. 2010. Toxicological effects of TiO2 and ZnO nanoparticles in soil on earthworm Eisenia fetida. Soil Biol. Biochem. 42:586-591.   DOI
14 ISO. 2012. Soil Quality Effects of Pollutants on Earthworms Part 1: Determination of Acute Toxicity to Eisenia fetida/Eisenia andrei. ISO 11268-1. International Organization for Standardization. Geneva, Switzerland.
15 Jankovic NZ and DL Plata. 2019. Engineered nanomaterials in the context of global element cycles. Environ. Sci. - Nano 6:2697-2711.   DOI
16 Jezequel D, J Guenot, N Jouini and F Fievet. 1995. Submicrometer zinc oxide particles: Elaboration in polyol medium and morphological characteristics. J. Mater. Res. 10:77-83.   DOI
17 Khan FR, A Laycock, A Dybowska, F Larner, BD Smith, PS Rainbow, SN Luoma, M Rehkamper and E Valsami-Jones. 2013. Stable isotope tracer to determine uptake and efflux dynamics of ZnO Nano- and bulk particles and dissolved Zn to an estuarine snail. Environ. Sci. Technol. 47:8532-8539.   DOI
18 Koen L and RJ Colin. 2001. Zinc and cadmium bodyburden in terrestrial oligochaetes: Use and significance in environmental risk assessment. Environ. Toxicol. Chem. 20:2067-2072.   DOI
19 Li WM and WX Wang. 2013. Distinct biokinetic behavior of ZnO nanoparticles in Daphnia magna quantified by synthesizing 65Zn tracer. Water Res. 47:895-902.   DOI
20 Laycock A, M Diez-Ortiz, F Larner, A Dybowska, D Spurgeon, E Valsami-Jones, M Rehkamper and C Svendsen. 2015. Earthworm uptake routes and rates of ionic Zn and ZnO nanoparticles at realistic concentrations, traced using stable isotope labeling. Environ. Sci. Technol. 50:412-419.
21 Ma H, PL Williams and SA Diamond. 2013. Ecotoxicity of manufactured ZnO nanoparticles - a review. Environ. Pollut. 172:76-85.   DOI
22 Makama S, J Piella, A Undas, WJ Dimmers, R Peters, VF Puntes and NW van den Brink. 2016. Properties of silver nanoparticles influencing their uptake in and toxicity to the earthworm Lumbricus rubellus following exposure in soil. Environ. Pollut. 218:870-878.   DOI
23 Nahmani J, ME Hodson, S Devin and MG Vijer. 2009. Uptake kinetics of metals by the earthworm Eisenia fetida exposed to field-contaminated soil. Environ. Pollut. 157:2622-2628.   DOI
24 Nowack B, FJ Ranville, S Diamond, JA Gallego-Urrea, C Metcalfe, J Rose, N Horne, AA Koelmans and SJ Klaine. 2013. Potential scenarios for nanomaterial release and subsequent alteration in the environment. Environ. Toxicol. Chem. 31:50-59.
25 OECD. 1984. Guidelines for the Testing of Chemicals No. 207: Earthworm Acute Toxicity Test. Organization for Economic Co-operation and Development. Paris.
26 OECD. 2016. Test No. 222: Earthworm Reproduction Test(Eisenia fetida/Eisenia andrei). Organization for Economic Co-operation and Development. Paris.
27 Rocha TA, NC Mestre, SMT Saboia-Morais and MJ Bebianno. 2017. Environmental behavior and ecotoxicity of quantum dots at various trophic levels: a review. Environ. Int. 98:1-17.   DOI
28 Shoults-Wilson WA, BC Reinsch, OV Tsyusko, PM Bertsch, GV Lowry and JM Unrine. 2011a. Role of particle size and soil type in toxicity of silver nanoparticles to earthworms. Soil Sci. Soc. Am. J. 75:365-377.   DOI
29 Romero-Freire A, S Lofts, J Francisco, M Peinado and AM van Gestel. 2017. Effects of aging and soil properties on zinc oxide nanoparticle availability and its ecotoxicological effects to the earthworm Esisennis Andrei. Environ. Toxicol. Chem. 36:137-146.   DOI
30 Salbu B, T Krekling and DH Oughton. 1998. Characterization of radioactive particles in the environment. Analyst 123:843-849.   DOI
31 Shoults-Wilson WA, BC Reinsch, OV Tsyusko, PM Bertsch, GV Lowry and JM Unrine. 2011b. Effect of silver nanoparticle surface coating on bioaccumulation and reproductive toxicity in Earthworms (Eisenia fetida). Nanotoxicology 5:432-444.   DOI
32 Tessier A, PGC Campbell and M Bisson. 1979. Sequential extraction procedure for the speciation of particulate trace metal. Anal. Chem. 51:844-851.   DOI
33 Wang Y and B Nowack. 2018. Dynamic probabilistic material flow analysis of nano-SiO2, nano iron oxides, nano-CeO2, nano-Al2O3, and quantum dots in seven European regions. Environ. Pollut. 23:589-601.   DOI