• Asian-Australasian Journal of Animal Sciences
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• v.23 no.11
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• pp.1503-1509
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• 2010

Forty-eight 40-wk-old Hi-sex laying hens were individually caged in an environmentally controlled house to evaluate the effect of garlic (Allium Sativum) juice administration on egg production, egg quality, and yolk cholesterol. Garlic juice was prepared by blending pealed garlic cloves with distilled water (1:1, w/w). Hens were randomly divided into four equal groups; one served as a control and the other three groups were individually gavaged, 3.75 ml, 7.5 ml, or 15 ml garlic juice, three times a week, which respectively represented 0.25, 0.50 and 1% of body weight. Egg production was recorded on a daily basis; egg weight, albumen height, albumen and yolk pH, Haugh unit, and bacterial count of E. coli-challenged eggs were recorded at day of oviposition (day-1) and after 5 and 10 days of storage at room temperature. Yolk cholesterol content was analyzed for five successive weeks. Garlic juice increased (p<0.05) egg weight and mass with no change in egg production intensity. Garlic juice administration recorded higher (p<0.05) albumen height and improvement in Haugh unit. Also, eggs from garlic-treated hens recorded lower (p<0.05) albumen and yolk pH when compared to eggs collected from control hens. Garlic reduced (p<0.05) the $log_{10}$ of bacterial count in egg contents linearly when challenged with E. coli. Egg-yolk cholesterol content was not influenced by garlic juice administration. It is concluded that garlic juice improved performance characteristics and may increase egg shelf life as indicated by egg quality improvement and lower bacterial count of E. coli-challenged eggs. The levels of garlic juice used in this study were insufficient to influence egg yolk cholesterol.

• Korean Journal of Poultry Science
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• v.29 no.1
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• pp.13-18
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• 2002

This study was conducted to investigate the effects of dietary activated coconut charcoal (ACC) on Performance, e99 quality and Plasma cholesterol level of Plasma in laying hens. One hundred forty four, 47-wk-old, ISA Brown commercial layers were used in a 28-d feeding trial after a 7-d adjustment Period. Four dietary treatments were 0, 0.5, 1.0 and 1.5e% levees of ACC supplemented to a corn-soybean meal basal diet. Egg Production was significantly increased as the levels of ACC increased. However, egg weight was significantly decreased by the addition of ACC in diets. Egg shell breaking strength tended to decrease as the level of ACC increased, however, no significance was found in this respect. As the levels of ACC increased, egg shell thickness decreased significantly. Although not significant, yolk color tended to increase by the addition of ACC. Egg folk index were significantly increased by the addition of ACC In diet. No significant difference was found among four treatments in total cholesterol, HDL cholesterol, and LDL+VLDL cholesterol concentrations in plasma. In conclusion, dietary supplementation of ACC to layer diets could be used to increase egg Production, and yolk index.

• Korean Journal of Poultry Science
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• v.21 no.1
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• pp.21-34
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• 1994

The objective of this study was to develop a user-friendly computer model for economic analysis on the commercial egg production that could help the egg farmer make managerial diagnosis and rational decision in the changing environment. To raise the adequacy of the model, the program was run for every sample and adjusted to fit the data. The model, programmed with Microsoft QuickBASIC, was a user-friendly computer program in supporting the Korean language. The basic analytical tool used in the study was an engineering-type computerized simulation model which incorporates a cost-benefit analysis of a full-time egg farmer. The computer model developed in this study may be the powerful analytical tool used to evaluate both a managerial decision whether to alter the production system and its impact on production, costs, revenue, and profits. Ultimately, the program is expected to enable the egg farmer to make managerial planning and diagnosis. The program can also calculate the values of economic variables at user-chosen incremental values of market eggs and feed prices. It provides the information on the profit and cost. This may lead the egg farmer, by allowing to establish the best managerial strategy, to increase the profit aor to lessen the cost. The results of this study could be utilized in the evaluation and improvement of the management. It also may be utilized for the researchers and guiding farmers in collecting and analyzing the data on the laying hen. In particular, such a program would be potentially useful to researchers who wish to quickly estimate profits associated with various laying hen treatments. The program could also benefit the egg farmer interested in making managerial decisions based on either current or predicted market conditions. The model would make the egg farmer respond actively to the information-oriented society by promoting to use personal computer.

• Journal of the Korea Organic Resources Recycling Association
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• v.14 no.4
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• pp.121-131
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• 2006

This study was designed to determinate the effects of dried leftover food and green tea by-product on laying hens performance and egg quality in hens. A total of 210 "Tetran Brown" layers 50-weeks of age were assigned to 7 treatments in a completely randomized design. Each treatment had five replicates per treatment with six layers per replication. Seven dietary treatments were a control diet (formula diet) and dried leftover food (DLF) mixed in 10, 20, 30 and 40% to the control diet substituting the corn grain and soybean meal, control diet containing 1.0% GTB without DLF supplementation and control diet containing 30% DLF plus 1.0% GTB supplementation. The trial period was for 8 weeks. The egg production rate of layers was significantly increased in 10, 20 and 40% DLF treatments compared to that of the control treatment (P<0.05). The egg weight was significantly decreased in 10% DLF treatment compared to that of the control (P<0.05). The feed intake of layers was higher in 20% DLF and 30% DLF plus 1.0% GTB treatment than that of the control (P<0.05). The feed conversion ratio significantly decreased in 10% DLF and control plus 1.0% GTB treatments compared to that of the control (P<0.05). The egg yolk cholesterol not varied among the DLF and control treatments (P>0.05). However, the linolenic acid content of egg yolk was significantly increased in DLF and control treatments both containing 1.0% GTB supplementation.

• Korean Journal of Poultry Science
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• v.29 no.2
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• pp.135-140
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• 2002

An experiment was conducted to determine the effects of vitamin C supplementation on the performance and egg quality of layers. A total of 800 ISA Brown layers of 71 weeks old were assigned to one of the following diets containing 0, 3, 10, 50 or 100 ppm LG-vitamin C, 100ppm free ascorbic acid, 100 ppm Rovimix stay C-35 or 50 ppm LG-vitamin C supplemented with 100 ppm Zn methionine chelate. The feeding trial was conducted for flour weeks during the period of May to June when the average ambient temperature was $23.8^{\circ}C$ (5 :00 PM). Each treatment was replicated five times with 20 birds housed in 2 birds cage units. Vitamin C supplementation did not improve the overall performances (egg Production and egg Quality). Birds fed 100 ppm of LG vitamin C had the lowest egg Production while birds fed 100 ppm of Rovimix-35 had the lowest egg weight among birds fed different sources of vitamin C. Although supplementation of vitamin C over 50 ppm increased soft and broken egg Production, supplementation of 10 ppm LG vitamin C showed lowest soft and broken egg Production among all treatments. E99she11 thickness was highest in the treatment of 50 ppm LG vitamin C. In conclusion, supplementation of vitamin C at the level of 3 ~ 100 ppm did not significantly influence the laying performance and eggshell quality of laying hens under normal ambient(20 ~$26^{\circ}C$ ) condition.

• Animal Bioscience
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• v.36 no.11
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• pp.1727-1737
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• 2023

Objective: The poultry industry is a primary source of animal protein worldwide. The gut microbiota of poultry birds, such as chickens and ducks, is critical in maintaining their health, growth, and productivity. This study aimed to identify longitudinal changes in the gut microbiota of laying hens from birth to the pre-laying stage. Methods: From a total of 80 Hy-Line Brown laying hens, birds were selected based on weight at equal intervals to collect feces (n = 20 per growth) and ileal contents (n = 10 per growth) for each growth stage (days 10, 21, 58, and 101). The V4 regions of the 16S rRNA gene were amplified after extracting DNA from feces and ileal contents. Amplicon sequencing was performed using Illumina, followed by analysis. Results: Microbial diversity increased with growth stages, regardless of sampling sites. Microbial community analysis indicated that Firmicutes, Proteobacteria, and Bacteroidetes were the dominant phyla in the feces and ileal. The abundance of Lactobacillus was highest on day 10, and that of Escherichia-shigella was higher on day 21 than those at the other stages at the genus level (for the feces and ileal contents; p<0.05). Furthermore, Turicibacter was the most abundant genus after changing feed (for the feces and ileal contents; p<0.05). The fecal Ruminococcus torques and ileal Lysinibacillus were negatively correlated with the body weights of chickens (p<0.05). Conclusion: The gut microbiota of laying hens changes during the four growth stages, and interactions between microbiota and feed may be present. Our findings provide valuable data for understanding the gut microbiota of laying hens at various growth stages and future applied studies.

• Korean Journal of Poultry Science
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• v.41 no.2
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• pp.93-98
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• 2014

This study was conducted to analysis the reason for implementing HACCP system, advantage and disadvantage of HACCP system implemented laying hen farm. The study was carried out by randomly selected fifteen laying hen farms located in all around Korea. All data were collected from fifteen laying hen farms before and after the implementation of HACCP system. The results were as follows: The egg production rate, livability rate and monthly used animal medicine fee did not changed after HACCP system implementation. However, monthly used disinfectant fee tended to be higher in HACCP farm compared to non-HACCP farm. 26.92% of the laying hen farmer responded enhancement of their farm competitiveness as the major propose for implementing HACCP system. The advantages of HACCP implemented laying hen farms were methodical farm management (22.39%), improvement of awareness (21.18%), improvement of the farm sanitation management level (15.30%), safety egg production (15.05%), productivity enhancement (7.29%), reduction of mortality rate (6.82%), and improvement of labor's welfare (5.89%). The disadvantages of HACCP implemented laying hen farms were HACCP recording (43.30%), alteration of consciousness (22.60%), HACCP monitoring (11.11%), HACCP education (9.97%), HACCP verification (6.90%), and A high turnover of labor (6.13%). In conclusion, implementation of HACCP system to laying hen farm did not affect on the productivity or the use of animal medicine. However, the HACCP system may enhance safety and sanitation of egg production for consumer.

• Korean Journal of Veterinary Service
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• v.28 no.3
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• pp.245-251
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• 2005

The purposes of this study were to evaluate the distribution of quinolone and to investigate the effects of quinolones (enrofloxacin, ciprofloxacin) in blood(plasma) and eggs of laying hens. Animals were fed quinolones which supplemented with 20, 50, 80 mg/kg of body weight. Blood and egg samples were collected after oral administration and analyzed for quinolones (enrofloxacin, ciprofloxacin) by HPLC. In laying hens, the residue period of enrofloxacin were longer than that of ciprofloxacin and the levels of residues were elavated by drug dosage.

• Korean Journal of Veterinary Research
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• v.58 no.4
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• pp.193-199
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• 2018

As animal welfare issue becomes important, the European Union bans conventional cages for laying hens from 2012. So the alternative housing systems like floor pens, aviaries or free range systems have been suggested. From 2011 to 2014, we monitored 20 welfare-oriented laying hen farms in South Korea to figure out serological status of major viral diseases. During this period, total 3,219 blood samples were collected from the randomly selected chickens to test and evaluate the hemagglutination inhibition titers for low pathogenic avian influenza, Newcastle disease and egg drop syndrome '76. A total of 2,926 blood samples were tested through enzyme linked immunosorbent assay (ELISA) to assess the serological status of infectious bronchitis (IB). The distribution of ELISA titers for IB was various from almost 0 to 20,000 through the all weeks of age. Also, the antibody coefficient of variation for most of the diseases in this study was higher than those of typical cage layers. As this study was the first surveillance for major avian viral diseases of the animal welfare-oriented farms in South Korea, the results obtained from this study will help to determine what information and resources are needed to maintain better biosecurity and to improve the health and welfare of laying hen flocks.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.391-397
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• 2015

Two hundred and sixteen Institut de S$\acute{e}$lection Animale (ISA) brown layers (40 wks of age) were studied for 6 wks to examine the effect of microalgae powder (MAP) on egg production, egg quality, blood lipid profile, and fatty acid concentration of egg yolk. Dietary treatments were as follows: i) CON (basal diet), ii) 0.5% MAP (CON+0.5% Schizochytrium powder), and iii) 1.0% MAP (CON+1.0% Schizochytrium powder). From 44 to 46 wks, egg production was higher in 1.0% MAP treatment than in control treatment (linear, p = 0.034); however, there was no difference on the egg production from 40 to 43 wks (p>0.05). Serum triglyceride and total cholesterol were significantly reduced in the groups fed with MAP, compared to those in groups fed with control diets (Quadratic, p = 0.034 and p = 0.039, respectively). Inclusion of 0.5% MAP in the diet of layers improved egg yolk color, compared with hens fed with basal diet at 46 wks (quadratic, p = 0.044). Eggshell thickness was linearly increased in MAP-fed treatments at 46th wk (p<0.05). Concentration of yolk docosahexaenoic acid (DHA; C22:6n-3) was increased in treatment groups fed with MAP (linear, p<0.05). The n-6 fatty acids, n-6/n-3 fatty acid, and unsaturated fatty acid/saturated fatty acid were decreased in treatment groups fed with MAP (linear, p<0.05). These results suggest that MAP improved the egg production and egg quality, and may affect serum lipid metabolites in the layers. In addition, MAP increases yolk DHA levels, and deceases n-6/n-3 fatty acid ratio.