• 한국축산식품학회지
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• 제43권2호
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• pp.282-304
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• 2023

This study aimed to investigate the effectiveness of carotenoid supplementation on the performance, egg quality, and immunity of laying hens using a meta-analysis approach. The database was searched using Google Scholar and Scopus, from 2012 to 2022. The literature was published in English. 47 Articles were selected for meta-analysis. Analyses were performed using the Open Meta-analyst for Ecology and Evolution (OpenMEE) software. The heterogeneity and data validation against publication bias were analyzed using JASP 0.16.2 software. Overall, the results showed that carotenoid supplementation improved feed intake by 0.32 g/day/hen [95% confidence interval (CI)=0.02 to 0.61], final body weight by 0.33 g/hen (95% CI=0.05 to 0.60), egg production by 0.38% (95% CI=0.14 to 0.63), egg weight by 0.29 g (95% CI=0.09 to 0.5), yolk colour by 2.11 (95% CI=1.71 to 2.51), Haugh unit (HU) by 0.26 (95% CI=0.11 to 0.42), yolk carotenoids by 1.17 ㎍/kg (95% CI=0.59 to 1.75), immunoglobulin A (IgA) by 0.74 mg/L (95% CI=0.18 to 1.29), and lower yolk cholesterol by -0.38 mg/g (95% CI=-0.59 to -0.16). Feed conversion ratio (FCR), eggshell thickness, and white blood cells were unaffected by the application of carotenoids. The heterogeneity analysis showed variability in all studies (<0.05). In conclusion, carotenoid supplementation can elevate productivity, enhance egg quality, and improve immunity. However, based on Kendall's test, there was a publication bias in several parameters, namely FCR, egg weight, HU, yolk carotenoids, and IgA.

• Toxicological Research
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• 제16권1호
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• pp.53-57
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• 2000

A mutagenicity testing system using a specific locus mutation of Bombyx mori was introduced from National Institute of Genetics in Japan. In the system, mutagenicity could be detected by the egg color manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. The efficiency to detect mutagenicity of the system was examined and improved. To promote the sensitivity of the system to mutagens, eight varieties of Bombyx mori were tested for their sensitivity to two mutagens, EMS and MMC. Two varieties of the silkworm, N12 and C5, were finally selected as the most sensitive ones. The most sensitive development stage of the silkworm to mutagens was mid and late pupal stages for female and male, respectively. The system will be applied to test unknown mutagens after some more detail examination about its sensitivity.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.1053-1058
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• 2008

A total of 392 twenty eight week-old laying hens was used to study the effects of dietary inclusion of solvent-extracted palm kernel cake (PKC) (0%, 12.5% and 25%) and enzyme (mixture of mannanase, ${\alpha}$-galactosidase and protease) supplementation (0 kg/t, 1 kg/t and 2 kg/t) on the performance of laying hens. The levels of PKC did not significantly influence nitrogen corrected true metabolizable energy (TMEn) of the diets. Enzyme-supplemented PKC had significantly higher AME and TMEn values than PKC diets with no enzyme supplementation. Dietary inclusion of 12.5% and 25% PKC in the diets of laying hens did not adversely affect mean egg production or daily egg mass. However, layers consumed significantly more PKC-based diets and had significantly poorer feed conversion ratios (FCR) than controls. However, the feed intake and FCR of hens provided the 12.5% PKC-based diets with enzyme supplementation at 1 kg/t did not differ from the controls. Dietary inclusion of PKC or enzyme did not affect eggshell quality, but egg yolk colour was significantly paler when layers were fed the 25% PKC diet.

• 한국응용곤충학회지
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• 제45권3호
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• pp.283-291
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• 2006

누에의 가시 돌연변이 형질을 이용한 환경변이원 검색계 수립을 위하여 MMC, EMS, PCB에 대한 누에알 변이원 검색계의 감수성을 조사한 결과, 난모세포계는 MMC, 정세포계는 EMS에 대하여 특정적으로 높은 감수성을 보였고, PCB와 dioxin에 대해서도 양성반응을 얻을 수 있었다. 수나방의 사정횟수에 따른 정세포의 변이원 감수성 변화를 시험한 결과, 1교와 3교 때 사정된 정자 간에는 큰 차이가 없었다. 변이원 감수성은 높으나, 산란성, 우화율 등 검색계로서 주요형질이 극히 불량한 선3호는 C5, Nl2, 한삼면과의 교잡으로 주요형질이 개선되고 높은 변이원 감수성을 유지하였다.

• Animal Bioscience
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• 제36권11호
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• pp.1709-1717
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• 2023

Objective: A pivotal study was designed to investigate the effect of Hydroxy (HYC) Cu, Zn, and Mn on egg quality and laying performance of chickens under tropical conditions. Methods: A total of 1,260 Babcock White laying hens (20-wk-old) were randomly assigned to one of 4 treatments with 15 replicates of 21 hens each in a Randomized Complete Block Design. The birds were reared for 16 weeks and were fed the corn-soybean meal diets supplemented with one of the following mineral treatments: T1, inorganic (INO, 15 ppm CuSO4, 80ppm MnSO4 and 80 ppm ZnO); T2, Hydroxy-nutritional level (HYC-Nut, 15 ppm Cu, 80 ppm Mn, 80 ppm Zn from Hydroxy); T3, Hydroxy-Low (HYC-Low, 15 ppm Cu, 60 ppm Mn, 60 ppm Zn from Hydroxy); T4, Hydroxy plus inorganic (HYC+INO, 7.5 ppm HYC Cu+7.5 ppm CuSO4, 40 ppm HYC ZnO+40 ppm ZnSO4, 40 ppm HYC Mn+40 ppm MnSO4). The egg production was recorded daily, while the feed consumption, feed conversion ratio (FCR) and egg mass were determined at the end of each laying period. The egg quality parameters were assayed in eggs collected over 48 h in each laying period. Results: Overall, no significant effect of treatments was observed on percent egg production, egg weight and FCR (p>0.05). Feed intake was significantly lower in birds fed Hydroxy plus inorganic (p<0.05) diet. The supplementation of HYC-Low significantly increased the egg mass compared to the other treatments (p<0.05). HYC supplementation alone or in combination with INO elicited a positive effect on shell thickness, shell weight, shell weight per unit surface area, yolk colour, albumen and yolk index for a certain period (p<0.05), but not throughout the whole laying period. Conclusion: Dietary supplementation of HYC-Low (15-60-60 mg/kg) showed similar effects on production performance and egg quality characteristics in laying hens as compared to 15-80-80 mg/kg of Cu-Zn-Mn from inorganic sources. This indicates that sulphate based inorganic trace minerals can effectively be substituted by lower concentration of hydroxyl minerals.

• Asian-Australasian Journal of Animal Sciences
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• 제26권6호
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• pp.831-837
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• 2013

An experiment was conducted to investigate the effects of dietary supplementation of Rhodobacter capsulatus KCTC-2583 on egg-yolk and serum cholesterol, egg production and quality parameters during the late laying periods in hens. A total of 160 Hy-Line Brown layers (54 wk-old) were randomly allotted to 4 treatment groups on the basis of laying performance. Each treatment had 4 replicates with 10 birds each (40 birds per treatment). Two hens were confined individually with cage size $35{\times}35{\times}40$ cm and each 10 birds (5 cages) shared a common feed trough between them forming one experimental unit. Dietary treatments were; basal diet supplemented with 0 (control), 0.05, 0.10 and 0.15% R. capsulatus KCTC-2583. Experimental diets were fed in meal form for 56 d. Dietary supplementation of increasing levels of R. capsulatus KCTC-2583 reduced (linear, p<0.05) egg-yolk cholesterol and triglycerides (d 28, 42 and 56) concentrations. Also, serum cholesterol and triglycerides (d 21, 42 and 56) concentrations were linearly reduced (p<0.05) with increasing dietary R. capsulatus KCTC-2583. Laying hens fed a diet supplemented with increasing levels of R. capsulatus KCTC-2583 had increased (linear; p<0.05) overall egg production, egg weight, egg mass and feed efficiency. However, dietary treatments had no effect (linear or quadratic; p>0.05) on feed intake of laying hens. At d 28 and 56, breaking strength and yolk colour of eggs were linearly improved (p<0.05) in laying hens fed dietary increasing levels of R. capsulatus KCTC-2583. Dietary treatment had no effects (linear or quadratic; p>0.05) on albumin height, shell thickness and shell weight at any period of experiment. These results indicate that dietary supplementation of R. capsulatus KCTC-2583 has the potential to improve the laying hen performance and lead to the development of low cholesterol eggs during late laying period in Hy-Line Brown hens.

• 한국잠사곤충학회지
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• 제13권2호
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• pp.119-122
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• 1971

체내 유존난중에 월년난 고유색으로 착색된 것의 유무와 교미를 필한 잠아를 직시처리구와 약간 산란후 처리구로 구분하여 산란공을 가열한 해부침으로 처리하여 산란을 임의 중절한 다음 일주간을 방치한 후 이를 해부하여 음도 수란관 및 난관기부에 있는 잠란을 각각 채취하여 그 수정여부를 조사 연구한바 다음과 같은 결과를 얻었다. 1. 공시아중 불과 5% 내외가 각각 1∼2개의 착색란을 보유하고 있을 뿐더러(Table 1.) 그 착색양상도 월년난고유 색채가 아니고 난관내 잠란의 일반적인 색채와 약간의 차이가 있는 일종의 이상 착색난에 지나지 않는다. 2. 교미 직후 직시처리구에서는 음도에 잠란이 유입되어 있지 않다. 3. 약간 산란후 처리구에서는 공시아수의 약 10%에 해당하는 잠아가 음도 또는 수란관에 1개식의 수정란을 보유하고 있다. 4. 교미 직후 처리구에서는 수란관에 공시아의 약 2%에 해당하는 잠아가 일개식의 수정란을 보유하고 있다. 5. 난관에는 전혀 수정란을 발견할 수 없다. 이는 정자가 난관에 까지 진출하지 못하는 것으로 인정하는 것이 타당할 것이다. 6. 교미직후 직시처리구에서는 대부분 잠난이 난관에 위치하고 있지만 약간 산란후 처리구에서는 산란으로 인하여 잠란의 산란공으로 향한 이동운동이 계속되어 수란관과 음도에 진출되어 있다. 7. 정자는 주로 수정양을 비롯한 음도 근처에 있으면서 이곳을 통과하여 산란공에 향하는 잠란에 부착하므로서 난문을 통하여 난문에 진입하는 것으로 믿어진다.

• 한국잠사곤충학회지
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• 제27권1호
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• pp.31-36
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• 1985

한성반문 및 한성란색 계통잠에 있어서 전좌염색체로 인한 생리적 결함이 전견중, 견층중 및 견층비율에 미치는 영향을 이들에 관한 성차를 지표로 하여 검토한 결과, 한성 및 비한성 계통견의 전견중의 성차 평균치는 각각 127%, 129%, 견층중의 성차는 각각 107%, 110%, 견층비율의 성차는 공히 85%였다. 이상의 결과에서 한성반문 및 한성 난색계통잠은 전견중, 견층중, 견층비율 등의 실용형질에 관한 한 전좌된 염색체편에 기인하는 생리적 결함은 극히 미소한 것으로 생각된다.

• Animal Bioscience
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• 제37권4호
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• pp.689-696
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• 2024

Objective: Our study examined the impact of propriety blends of Bacillus strain probiotics on the performance, egg quality, and faecal microflora of laying hens. Methods: A total of 183 Institut de selection Animale (ISA) brown laying hens aged 23 weeks with an average body weight of 1,894±72 g were randomly allocated into 3 groups as control (corn-soybean meal based diet, CON), 0.5 g/kg Enterosure probiotics (ET1, 3×10⁸ colony-forming unit [CFU]/kg feed), and 5 g/kg Enterosure probiotics (ET2, 3×10⁹ CFU/kg feed) administered in mashed form. At the completion of each phase hen day egg production (HDEP), average egg weight (AEW), feed intake, and faecal microbiota were evaluated. Results: HDEP and AEW were higher (p<0.05) in the ET2-supplemented diet in phase 3 (week 9 to 12) compared with CON. Egg mass (EM) was higher (p<0.05) in phase 2 at ET2, and also higher (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets compared with CON. Feed conversion ratio was lower (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets, with ET2 being the lowest compared with ET1 and CON. Yolk colour was higher (p<0.05) in the ET-supplemented diets at phase 3 compared with CON. Bifidobacterium spp. was higher (p<0.05) in the ET2- supplemented diet compared with CON in phase 2, while in In phase 3, Lactobacillus spp. and Bifidobacterium spp. were higher (p<0.05) in the ET-supplemented diets compared with CON. Coliforms were lower (p<0.05) in the ET-supplemented diets compared with CON in phase 3. Conclusion: The propriety blends of Bacillus strain probiotics supplements at 0.5 g/kg and 5 g/kg could improve the production and quality of eggs with more significance at 5 g/kg for HDEP, AEW and EM, which was achieved via the increase of beneficial microbiomes such as Lactobacillus spp., Bifidobacterium spp., and the decrease of pathogenic microbiomes like Escherichia coli and Coliforms which was speculated to improve gut barrier function and the reproductive hormone.

• 한국잠사곤충학회지
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• 제49권1호
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• pp.1-7
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• 2007

누에의 가시 돌연변이 형질을 이용한 환경변이원 검색계의 연중검색 체계수립을 위하여 인공사료누에의 환경변이원에 대한 적품종 및 감수성을 조사하였다. 변이원에 대한 인공사료육 누에의 적합 품종 조사를 위해 누에품종 AT, 백옥잠, C5와 N12를 대상으로 변이원 MMC를 투여한 후 주요형질을 조사한 결과, AT는 암나방의 우화율에서, C5와 N12는 수나방의 수정률에서 높은 성적을 보였다. 반면에 백옥잠은 암나방 우화율, 암수나방의 정상산란비율, 암나방의 산란수 및 수정률에서 높은 성적을 나타내어 인공사료에 대한 적품종으로 선발되었다. 또한 변이원에 대한 감수성 조사 결과, 인공사료육에 적품종으로 선발된 백옥잠은 공시된 다른 품종보다 다소 감수성이 낮았으며, AT가 누에의 환경변이원 검색 시스템에 높은 감수성을 보였다. 변이원 감수성은 높으나, 산란성, 우화율 등 검색계로서 주요형질이 극히 불량한 AT를 연중 검색 체계수립을 위한 변이원 검색계로 이용하기 위해서는 백옥잠 등과의 교잡으로 주요형질을 개선할 필요가 있다고 판단되었다.