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Improvement of a Screening System for Environmental Mutagens by Means of a Specific Locus Mutation of the Silkworm, Bombyx mori  

Yoon, Hyung-Joo (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
Kim, Sam-Eun (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
Kim, Jong-Gill (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
Choi, Ji-Young (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
Publication Information
Korean journal of applied entomology / v.45, no.3, 2006 , pp. 283-291 More about this Journal
Abstract
The efficiency to detect mutagenicity of the system using a specific locus mutation of Bombyx mori was examined and improved. In the system, mutagenicity could be detected by the egg colour manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. Among tested four mutagens, MMC had specially high sensitivity in the oocytes of silkworm and EMS had in the spermatozoa. PCB and dioxin showed a positive effect in both the oocytes and spermatozoa. In a consequence of sensitivity of mutagen by mating number of male moth of B. mori, treated mutagen, there was no difference between one mating - and three mating - male moth in sensitivity of mutagen. Sun3ho, B. mori variety, which showed high sensitivity to mutagens was improved in the major characteristics by crossing of C5 and N12.
Keywords
Mutagenicity; Bombyx mori; Specific locus mutation; Egg colour;
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