Browse > Article

The Selection and Sensitivity to Environmental Mutagen of Silkworm Reared Artificial Diet in a Screening System Using Specific Locus Mutation of the Silkworm, Bombyx mori  

Yoon, Hyung-Joo (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
Kim, Sam-Eun (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
Kim, Jong-Gill (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
Choi, Ji-Young (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
An, Mi-Yong (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
Publication Information
Journal of Sericultural and Entomological Science / v.49, no.1, 2007 , pp. 1-7 More about this Journal
Abstract
We investigated the selection and sensitivity to environmental mutagen of silkworm reared artificial diet to develop all-year-round operation system using a specific locus mutation of Bombyx mori. In the system, mutagenicity could be detected by the egg colour manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. Among, hi, Backokjam, C5, and N12, varieties of silkworm, AT was higher than other varieties in eclosion rate of female, and C5 and N12 were higher in fertility of male. Bakokjam was higher in eclosion rate of female, rate of moth to lay eggs normally in female and male, no. of eggs layed in female and fertility of female. As above results, Bakokjam was finally selected as the most fitness one among varieties of silkworm reared artificial diet. But the sensitivity to mutagen was lower than other varieties. In the sensitivity to mutagen, AT was the most sensitivity to mutagen in tested varieties of silkworm. To use AT variety in this system, AT was improved major characteristics, eclosion rate, fertility, rate of moth to lay eggs normally, and so on, by crossing of other varieties including Bakokjam.
Keywords
Mutagenicity; Bombyx mori; specific locus mutation; egg colour; artificial diet rearing;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Aisemberg, J., D. E. Nahabedian, E. A. Wider and N. R. Verrengia Guerrero (2005) Comparative study on two freshwater invertebrates for monitoring environmental lead exposure. Toxicol. 210: 45-53   DOI   ScienceOn
2 Fernandez-Bayon, J. M., J. D. Barnes, J. H. Ollerenshaw and A. W. Davison (1993) Physiological effects of ozone on cultivars of watermelon (Citrullus lanatus) and muskmelon (Cueunis melo) widely grown in Spain. Environ. Poolut. 81: 199-206   DOI   ScienceOn
3 Kuroda, Y (1995) Retrieval method of anti-mutagen and -cancer materials. 124-151 pp. Chungdamsa scientific publishing company, Japan
4 Murakami, A (1984) Dose-response relationships for mutation induced by chemicals in the silkworm. Problems of theshold in chemical mutagenesis. 5- 13
5 Yoon, H. J., S. E. Kim, J. K. Kim and J. Y. Choi (2006) Improvement of a screening system for environmental mutagens by means of a specific locus mutation of the silkworm, Bombyx mori. Korean J. Appl. Entomol. 45: 1-9   과학기술학회마을
6 Maron, D. M. and B. B. Ames (1983) Revised methods for the Salmonella mutagenicity test. Mutation Res. 113: 173-215   DOI
7 Cooper, C. S. and P. L. Grover (1990) Chemical carcinogenesis and mutagenesis I. 1-29 pp. Springer-Verlag publishing company, Berlin
8 Wurgler, F. E. and E. W. Vogel (1985) Chemical Mutagens. In vivo mutagenicity tests using somatic cells of Drosophila melanogaster eds by FJ. de Serres. 1-72 pp. Pleum Press, New York
9 Tazima, Y (1978) Mutagenicity testing of environmental chemicals in the silkworm : an important laboratary tool, 247.268 pp. Kodasha publishing company, Tokyo
10 Kim S. E. M. Y. Ahn, H. J. Yoon, J. G. Kim and J. I. Choi (2000) A screening system for environmental mutagens by means of specific locus mutation of the silkworm, Bombyx mori. J. toxicol. Pub. Health. 16: 53-57
11 Murakami, A (1983) Characteristics on mutagenicity test of environmental chemicals using insects. Environ. Mutation Res. 5: 12-21
12 Kuroda, Y (1989) Lectures on method of animal genetics. 124-151 pp. Konglib publishing company, Jappan
13 Gee, P., D. M. Maron and B. M. Ames (1994) Detection and classification of mutagens: a set of base-specific Salmonella tester strains. Proc. Natl. Acad. Sci. 91: 11606-11610
14 Larsen K. J., S. R. Brewer and D. H. Taylor (1994) Differential accumulation of heavy metals by web spiders and ground spiders in an old field. Environ. Toxicol. Chemj. 13: 503-508   DOI
15 Dean, B. J. and N. Danford (1984) Mutagenicity testing. In assays for the detection of chemically induced chromosome damage in cultured mammalian cells, eds. by Venitt, S. and Parry, J. M. 187-232 pp. IRL Press, Oxford
16 Hayashi, M., T. Morita, Y. Kodama, T. Sofuni and M. Jr. Ishidate (1990) The micronucleus assay with mouse peripheral blood reticulonocytes using acridine orange-coated slides. Mutation Res. 245: 245-249   DOI   ScienceOn