Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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The Selection and Sensitivity to Environmental Mutagen of Silkworm Reared Artificial Diet in a Screening System Using Specific Locus Mutation of the Silkworm, Bombyx mori

누에의 가시 돌연변이형질을 이용한 인공사료육 누에의 적품종 및 변이원 감수성 조사

  • Yoon, Hyung-Joo (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Sam-Eun (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Jong-Gill (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • Choi, Ji-Young (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • An, Mi-Yong (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
  • 윤형주 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 김삼은 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 김종길 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 최지영 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 안미영 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과)
  • Published : 2007.06.30
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Abstract

We investigated the selection and sensitivity to environmental mutagen of silkworm reared artificial diet to develop all-year-round operation system using a specific locus mutation of Bombyx mori. In the system, mutagenicity could be detected by the egg colour manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. Among, hi, Backokjam, C5, and N12, varieties of silkworm, AT was higher than other varieties in eclosion rate of female, and C5 and N12 were higher in fertility of male. Bakokjam was higher in eclosion rate of female, rate of moth to lay eggs normally in female and male, no. of eggs layed in female and fertility of female. As above results, Bakokjam was finally selected as the most fitness one among varieties of silkworm reared artificial diet. But the sensitivity to mutagen was lower than other varieties. In the sensitivity to mutagen, AT was the most sensitivity to mutagen in tested varieties of silkworm. To use AT variety in this system, AT was improved major characteristics, eclosion rate, fertility, rate of moth to lay eggs normally, and so on, by crossing of other varieties including Bakokjam.

누에의 가시 돌연변이 형질을 이용한 환경변이원 검색계의 연중검색 체계수립을 위하여 인공사료누에의 환경변이원에 대한 적품종 및 감수성을 조사하였다. 변이원에 대한 인공사료육 누에의 적합 품종 조사를 위해 누에품종 AT, 백옥잠, C5와 N12를 대상으로 변이원 MMC를 투여한 후 주요형질을 조사한 결과, AT는 암나방의 우화율에서, C5와 N12는 수나방의 수정률에서 높은 성적을 보였다. 반면에 백옥잠은 암나방 우화율, 암수나방의 정상산란비율, 암나방의 산란수 및 수정률에서 높은 성적을 나타내어 인공사료에 대한 적품종으로 선발되었다. 또한 변이원에 대한 감수성 조사 결과, 인공사료육에 적품종으로 선발된 백옥잠은 공시된 다른 품종보다 다소 감수성이 낮았으며, AT가 누에의 환경변이원 검색 시스템에 높은 감수성을 보였다. 변이원 감수성은 높으나, 산란성, 우화율 등 검색계로서 주요형질이 극히 불량한 AT를 연중 검색 체계수립을 위한 변이원 검색계로 이용하기 위해서는 백옥잠 등과의 교잡으로 주요형질을 개선할 필요가 있다고 판단되었다.

Keywords

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