• Journal of Nutrition and Health
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• v.41 no.1
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• pp.41-53
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• 2008

Gestational diabetes mellitus (GDM) is defined as glucose intolerance discovered or onset during pregnancy and attention is needed because of increased risk of perinatal morbidties and higher incidence of diabetes afterward. This study was performed to identify dietary factors associated with the development of gestational diabetes mellitus (GDM). Developed food frequency questionnaire containing 192 food items were used to assess nutritional status of 246 control subjects and 104 GDM subjects. Food habits of subjects were examined in the questionnaire. The more irregularity and less variety of meal were found in GDM group compared to control group and GDM group tended to eat rapidly and do not like vegetables and greasy foods. Total score of food habits in GDM was lower than control group, which suggests that GDM group have undesirable food habits. Mean daily energy and carbohydrate intakes of GDM group were higher than those of control group, and percent energy from protein was significantly higher in control. Nutrient density of protein, calcium, phosphorus and vitamin BI of GDM group was significantly lower than those of control group. Therefore dietary quality of GDM group was lower than that of control group. Odds ratio for GDM was high when energy and carbohydrate intakes were high. And when the intakes of rice, noodle, shiruduk, hamburger, boiled egg, steamed pork shank were high, the odds ratio for GDM was high. These results indicate that the amount and frequencies of several foods and dish items were related with the occurrence of GDM subjects. On the whole, GDM subjects consumed more cereals and less vegetables and less legumes. From these results, pregnant women with GDM tended to have unhealthy food habits, and carbohydrate intake was important dietary factors on the onset of GDM.

• The Korean Journal of Community Living Science
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• v.20 no.3
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• pp.423-435
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• 2009

Meal menus were developed and applied for rural elderly of Gangwon and Chungnam areas at agricultural off-season. Amounts of the major nutrients of carbohydrate, protein, lipid, and calorie considered in the menu based on the dietary reference intakes(DRI) of the elderly aged over 65 years were 97.5g, 22.5g, 13.3g, and 600kcal, respectively. In Chungnam, softness and overall acceptance of rice in menu 4, consisted of cereals and rice, kimchi stew with tuna, pan-fried bulgogi mushroom, seasoned dropwort carrot, kimchi, and banana, scored the highest values of 8.5 out of 9.0point hedonic scale (p<0.05). In Gangwon, soup and side dish of menu 3 (rice, frozen pollack pot stew, meatball fried in egg, seasoned sea lettuce, and banana) scored significantly the highest of all (p<0.05). Overall acceptance of all the menus were high over 7.8 without significance in Chungnam. Overall acceptance of the menu 3 scored significantly the highest with value of 8.5 and menu 4 (rice, spicy chicken soup, seasoned bean sprouts, broiled brown seaweed trunk, kimchi, tomato) had significantly the lowest value with value of 7.8 in Gangwon(p<0.05). The total leftover volume of menu 4 was significantly the highest with score of 56g and the menu 4 showed the highest unit cost of 2,900won in Chungnam. However, the total leftover volume of all menus did not show significance ranging from 9g to 19g in Gangwon.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.6
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• pp.586-593
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• 1985

The rose bitterling, Rhodeus ocellatus (KNER) is commonly distributed in the fresh waters of Korea and Japan. On January 15, February 15, and March 18 in 1984, mature adults of rose bitterling were caught in the watercourse of Maeri, Kimhae, South Korea. The authors fertilized the eggs employing dry method in the laboratory on May 7, 16 and 25 in 1984. Hatched larvae were reared in small aquariums at $17{\sim}25.5^{\circ}C$ (average around $21.2^{\circ}C$). Mollusks, Anodonta woodiana in the gill chamber of which, rose bitterling lay eggs were caught in order to study natural spawning of the rose bitterling in the same watercourse. The eggs of this species are not adhesive and demersal. The size of the eggs varies from 2.54 to 2.75mm in long diameter and 1.45 to 1.65mm in short diameter. The eggs are cylindrical in form when they are extruded from ovipositor, immediately after entering water, but they acquire their distinctive form of a greatly elongated pear. Hatching took place in ca. 39 hours after fertilization. The newly hatched larvae were $2.65{\sim}2.70mm$ in total length possessing yolk sac and 13-14 myotomes. Thirteen days after hatching, the prelarvae attained 6.5 mm in total length, and the first melanophores appeared on the head, and the anterior part and sides of the yolk sac. One month after hatching, the postlarvae attained 8.5mm. in total length and emerged from the gills of the mollusks. Then the yolk sac was completely resorbed. Two months after hatching, the rose bitterling attained 14.4mm in total length, and entered the fingerling period of life. All the rays already present were the D. III, 11-12, A. III, 11-12, P. 10, V. 7. and a distinguishing, feature is the presence of a black pigment spot in the lobe of the dorsal fin.

• Korean Journal of Poultry Science
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• v.44 no.2
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• pp.93-102
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• 2017

A duplex RT-PCR (dRT-PCR) assay was developed for the simultaneous detection and discrimination of non-virulent and virulent Newcastle disease virus (NDV) in a single PCR tube. Primers targeting the large polymerase protein (L) gene and the fusion protein (F) gene of NDV were designed to detect all NDVs (by common type PCR primers) and virulent NDVs (by pathotype PCR primers), respectively and evaluated experimentally with reference NDV strains and other poultry viral pathogens. PCR products of the expected size of 386 bp were amplified from all NDV samples whereas PCR products of the expected size of 229 bp were amplified from virulent NDV samples alone. Cross reaction was not observed with other avian viral pathogens. The detection limit of NDV by the dRT-PCR was estimated to be $10^3$ 50% egg infectious dose/0.1 mL. In the dRT-PCR using field isolates of NDV, the pathotype PCR primers detected specifically all of virulent field isolates of NDV from Malaysia, Pakistan and China whereas common type PCR primers detected 94.4% (51/54) of field isolates of NDV from China. Three Chinese NDV isolates with false negative result were non-virulent viruses. Our results indicate that the dRT-PCR might provide a rapid and simple tool for rapid simultaneous detection and discrimination of non-virulent and virulent NDVs. Therefore the developed dRT-PCR assay provides a powerful novel means for the rapid diagnosis of Newcastle disease.

• Journal of the East Asian Society of Dietary Life
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• v.12 no.2
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• pp.107-122
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• 2002

The purpose of this study was to develop menus for daily intake of 25g soybean protein and to analyse nutrients of these foods. Analytical values were compared to the theoretical one using the flood composition table and recommended dietary allowances for Koreans (7th revision). The results are as follows. 1. Soybean curd residue stew, Soybean curd, Kimchi saute, and hard boiled soybean and lotus root were selected for the menu for January, of which the content of soy bean protein(SBP) was 33.1g, soybean stew, soybean curd and soybean sprout saute, and Italian deep fried soybean curd were fur February, of which the content of SBP was 35.0g. The content of SBP in soybean paste soup with soybean curd, fried soybean curd and fried soybean curd roll, the menu for March, was 24.9g. That of April were soybean curd gratin with soymilk, soybean curd and ham with garlic dressing and the content of SBP was 26.3g. That of May were soybean porridge, soybean flour cake with honey (Dasik), soybean sprout soup and the content of SBP was 26.7g. That of June were soymilk, pan-fried soybean curd, steamed soybean curd with chicken and the content of SBP was 28.4g. That of July were noodle with soymilk, mapatofu, soybean curd salad and the content of SBP was 24.7g. That of August were soybean sprout with mustard dressing, Tossed green pepper with raw soybean flour, Tofu and Kimchi stew, soybean curd steak and the content of SBP was 26.2g. That of September were Chinese cabbage soup with raw soybean flour, sweet and sour tofu and the content of SBP was 23.2g. That of Oct. were Fermented soybean stew, soybean pan cake and the content of SBP was 24.3g. That of November were not-pressed soybean curd casserole, pan-fried mashed soybean curd with egg, stir frying deep-fried soybean curd with vegetables and the content of SBP was 22.4g. That of December were soybean curd and mushroom casserole, fried soybean curd and vegetables, hard boiled soybean curd and the content of SBP was 28.9g. 2. The ratio of the analytical value over theoretical value (A/B%) of one serving in kcal, carbohydrate, protein, fat ash and dietary fiber were 57.7~107.7% 42.9~131.9%, 79.2~118.3%, 54.5~ 100%, 40.7~80.8% and 42.1~113.2%, respectively.

• Korean journal of applied entomology
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• v.44 no.4 s.141
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• pp.293-298
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• 2005

The embryonic and postembryonic developments of Pierodorus hybneri Linnaeus were observed in 5 different rearing cages such as A (Cylindrical, 10 cm in diameter, height of 4 cm), B (Cylindrical, 14.5 cm in diameter, height of 2.8 cm), C (Rectangle, 6.5 by 6.5 cm in $length{\times}breadth$, height of 10 cm), D (Cylindrical, 9 cm in bottom diameter, 11.5 cm in upper diameter, height of 10.8 cm) and E (Cylindrical, 15 cm in diameter, height of 7.5 cm) containing soybean and peanut seeds as feeding food, and sponge-water container under laboratory condition of $24^{\circ}C$ and 16L:8D. Egg duration was 6 days regardless of rearing cages. Hatchability ranged from 63 to 80% with the highest in B (14.5 cm in diameter, 2.8 cm in height) rearing cage. Instar duration was longer from 5 days in 1st instar to about 11 days in 5th instar. Nymphal duration showed 35 to 36 days with'3ut significant difference in rearing cages. Percent emergence was in range from 65 to 82% with the highest in B rearing cage. Adult longevity was 35 to 83 days for male, and 32 to 79 days for female, and was the longest in B rearing cage. Total number of eggs laid by female adult was in range from 38 to 86 with significant difference in rearing cages, and was the most in B rearing cage. Accordingly, the reproductive rate of P. hybneri for 1 generation was within 17 to 56 times, and was the highest in B rearing cage. Therefore, it could be concluded that B cage is most suitable for stable rearing of P. hybneri under laboratory condition.

• Korean journal of applied entomology
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• v.24 no.3 s.64
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• pp.151-156
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• 1985

Apple leaf miner(ALM), Phyllonorycter ringoniella(Matsumura), occurs $4{\sim}5$ generations a year in Suweon; adult emergence peaks being in mid April, early June, early July, mid August, and mid September with the highest one in the 4th generation. Numbers of days required to complete the development(egg to adult emergence) were different with oviposited dates; 43days for late May, $32{\sim}37$ days for June to July, and 39days for early August. ALM larvae oviposited after late August did not emerge and went into diapause. Sites of the infested leaf on the shoot seemed to be somewhat different with the ALM generations; the preferred leaf sites being the 1st-6th leaf for the 1st, the 4th-9th for the 2nd, the 4th-18th for the 3rd or 4th, and the terminal leaves of the first growth shoot or the leaves of the secondary shoot for the 5th generation. Parasites of three families emerged from the mines of ALM in Suweon. The encyrtid (Holcothorax testaceipes Ratzelburg) occurs 4 generations and the eulophids 5 generations a year. A small number of the braconid(Apanteles sp.) occcrred only in September.

• Korean journal of applied entomology
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• v.24 no.4 s.65
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• pp.195-201
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• 1986

This study was conducted to investigate the life history of Compsidia populnea which is major species of the stem-borers on Populus alba{\times}glandulosa$. Peak emergence of the adult of Compsidia populnea L. was around 10th${\sim}$l2nd of May in Chuncheon vicinities, having one generation a year in Korea. There was a preovipositional period of 10.7 days and a ovipositional period of 14.3.days. Mean adult longevity was 11 days for male and 13.8 days for female. Individual eggs were long oval with somewhat narrowed tip at one side and soft, 2.6mm in length, 0.8mm in diameter, and were laid singly under the U-shaped scars which were made by female before oviposition. Total number of scars per female averaged 56.6 ($8{\sim}135$) and eggs were found in 67.9 percent of the total scars. Egg period was $8{\sim}11$ days at $25^{\circ}C$ constant temperature and $7{\sim}14$ days in the field condition. Early young larvae stayed for $2{\sim}3$ weeks under the scars and then boring into the xylem, forming galls at this time. Size of the gall was 1.8cm($1.3{\sim}2.5$) in length, 1.6cm($1.0{\sim}2.2$) in diameter and the length of mine was everage 3.1cm. Fully grown larvae were developed around the early October and overwintered in the mines of galls. Pupation was done around the early of April. Pupal developmental period averaged 11 days($9{\sim}13$ days) at $25^{\circ}C$ constant temperature condition.

• Korean journal of applied entomology
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• v.26 no.2 s.71
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• pp.71-81
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• 1987

This study was conducted to study the effects of temperature on the oviposition, feeding and emergence of the azuki bean weevil(ABW) (Callesobruchus chinensis L.) in the stored beans. And ovipositional preference, hatching and emergence on the preference and non-preference hosts were also investigated. ABW has four generations a year and overwinters as larva stage in the bean grains. Optimum oviposition temperature of the ABW ranges from $25^{\circ}C\;to\;30^{\circ}C$. Oviposition preference in different hosts was in the order of azuki bean, mung bean, soy bean, kidney bean, black-soybean, pea. At $25^{\circ}C$, optimum development temperature emergence of period the ABW averaged 29 days on azuki bean, 31 days on mung bean, 49 days on soy bean, 46 days on black-soybean, 34 days on Pea. Percent hatch in different hosts was in the order of azuki bean, mung bean, soy bean, pea, black-soybean, kidney bean. But percent emergence was in the order of azuki bean, mung bean, pea, black-soybean, kidney bean. Especially, in azuki bean and mung bean, percentage of hatching and emergence was very high. And in soy bean and black-soybean, percentage of hatching was high but emergence was low. The kidney bean was hatching but not emergence at all. The longevity at the different temperatures appeared to be $4{\sim}5$ days at $35^{\circ}C,\;5{\sim}7$ days at $25^{\circ}C{\sim}30^{\circ}C$, $10{\sim}15$ days at $20^{\circ}C\;and\;19{\sim}24$ days at $15^{\circ}C$. Kidney bean of feeding non-preference host oviposited at Seed coat and Endosperm, but no larva developed. And cotyledon and embryo parts were not oviposited at all. The percentage of germination of egg laid bean grain and damaged bean grains was low. Especially, the percentage of germination of damaged bean grains was 0% on azuki bean, 5% on black-soy bean, 7% on Pea.

• Tuberculosis and Respiratory Diseases
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• v.56 no.3
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• pp.261-268
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• 2004

Background : The resurgence of tuberculosis and outbreaks of multidrug resistant (MDR) tuberculosis have increased the emphasis for the development of new susceptibility testing of the Mycobacterium tuberculosis for the effective treatment and control of the disease. Conventional drug susceptibility testings, such as those using egg-based or agar-based media have some limits, such as the time required and difficulties in determining critical inhibitory concentrations, but these are still being used in many diagnostic laboratories because of no better lternatives, considering cost and accuracy. To overcome these limits, a rapid and simple method for new susceptibility testing, using live and dead assays, was applied for a bacterial cell viability assay to distinguish dead from live bacterial cells based on two-color fluorescence. Materials and Methods Strains : Forty strains were used in this study, 20 susceptible to all antituberculosis drugs and the other 20 resistant to the four first line antituberculosis drugs isoniazid, rifampicin, streptomycin and ethambutol. Antibiotics : The four antibiotics were dissolved in 7H9 broth to make the following solutions: $0.1{\mu}g\;isoniazid(INH)/m{\ell}$, $0.4{\mu}g\;rifampicin(RMP)/m{\ell}$, $4.0{\mu}g\;streptomycin(SM)/m{\ell}$ and $4.0{\mu}g\;ethambutol(EMB)/m{\ell}$. Results : Live and dead Mycobacterium tuberculosis cells fluoresced green and red with the acridin (Syto 9) and propidium treatments, respectively. These results are very well accorded with conventional drug susceptibility testing by proportional method on Lowensen-Jensen media (L-J) containing 4 drugs (INH, RMP, EMB and SM), showing a 93.7 % accordance rate in susceptible strains and 95% in resistant strains. Conclusion : The results of the drug susceptibility testing using the live and dead bacterial cell assay showed high accordance rates compared with the conventional proportion method on L-J. This finding suggests that the live and dead bacterial cell assay can be used as an alternative to conventional drug susceptibility testing for M. tuberculosis strains.