• Korean Journal of Environment and Ecology
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• v.28 no.3
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• pp.357-364
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• 2014

This study aimed to determine effects of the use of water-retention additive, hydrophilic polymer, for extensive green roofs on growth of Juniperus chinensis var. sargentii and Euonymus fortunei 'Emerald and Gold' for woody plants, and Carex kobomugi and Carex pumila for herbaceous plants. Five different contents of hydrophilic polymer including 0% (Control), 1.0%, 2.5%, 5.0%, and 10% (polymer: medium (w/w), dry weight basis) were added to each of the container filed with a 100 kg of growth medium. Ten of plants were transplanted in each of square container ($1m(L){\times}1m(W){\times}0.3m$ (H)) built on the roof platforms in randomized complete block design in the $20^{th}$ of May, 2013. In results, excessively high volumetric soil water content, about 97-98%, was found in the substrate under elevated hydrophilic polymer concentration of at least 2.5%, during the entire growing period. The moisture content of the substrate containing 1.0% of hydrophilic polymer was higher about 20% in the range between 70% and 80%, compared tho that of Control substrate in the range between 50% and 60%, for 27 days after transplanting prior to abundant rainfall, indicating that the application of hydrophilic polymer to the extensive green roof substrate is effective to eliminate drought condition by retaining water in the substrate. Euonymus fortunei 'Emerald and Gold' and Carex kobomugi resulting in higher plant growth with 2.5% than those of the other treatment plants. Juniperus chinensis var. sargentii was observed the highest growth under 1.0% hydrophilic polymer treatement, and Carex pumila was founded the best growth with Control respectively. Plants that grown in both the 1.0% and 2.5% hydrophilic polymer survived all, while the plants that grown in the 5.0% and 10% hydrophilic polymer died after 3 months. These results suggest that advantage of the addition of hydrophilic polymer may be greater in drought-tolerant plants, but the mixture proportion of hydrophilic polymer should be determined according to the different features of the plant species being grown.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.12
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• pp.1750-1756
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• 2011

This study evaluated the effect of antioxidants treatment; (Vit. C and E) on the physicochemical and sensory properties of Tarakjuk treated with gamma-irradiation under two different temperature conditions, e.g. room and frozen temperatures. In samples of Tarakjuk irradiated at room temperature, Vit. C gradually decreased the pH level as the concentration increased (p<0.05), whereas Vit. E did not. Addition of both Vit. C and E to Tarakjuk decreased TBA (2-thiobarbituric acid) value while also increasing viscosity. However, these antioxidants did not cause any change in Hunter's color values. Sensory properties showed that Vit. C and E significantly improved the overall acceptability of Tarakjuk, and the addition of 0.1% each Vit. C and E was best treatment. In samples of Tarakjuk subjected to combined treatment with antioxidants and irradiation under frozen temperature conditions, Vit. C and E were effective in decreasing TBA value, improving viscosity, and maintaining sensory quality. Especially, co-treatment with 0.1% Vit. C and E augmented the observed effect (p<0.05). Therefore, combined treatment with antioxidants and irradiation under frozen temperature conditions could improve the final sensory quality of gamma-irradiated Tarakjuk.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.161-168
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• 2014

This study aimed to investigate dietary concentrate:forage ratios (C:F) and undegraded dietary protein (UDP) on nitrogen balance and urinary excretion of purine derivatives (PD) in lambs. Four Dorper${\times}$thin-tailed Han crossbred castrated lambs with $62.3{\pm}1.9$ kg body weight at 10 months of age were randomly assigned to four dietary treatments in a $2{\times}2$ factorial arrangement of two levels of C:F (40:60 and 60:40) and two levels of UDP (35% and 50% of CP), according to a complete $4{\times}4$ Latin-square design. Each experimental period lasted for 19 d. After a 7-d adaptation period, lambs were moved into individual metabolism crates for 12 d including 7 d of adaption and 5 d of metabolism trial. During the metabolism trial, total urine was collected for 24 h and spot urine samples were also collected at different times. Urinary PD was measured using a colorimetric method and creatinine was measured using an automated analyzer. Intake of dry matter (DM) (p<0.01) and organic matter (OM) (p<0.01) increased as the level of UDP decreased. Fecal N was not affected by dietary treatment (p>0.05) while urinary N increased as the level of UDP decreased (p<0.05), but decreased as dietary C:F increased (p<0.05). Nitrogen retention increased as dietary C:F increased (p<0.05). As dietary C:F increased, urinary excretion of PD increased (p<0.05), but was not affected by dietary UDP (p>0.05) or interaction between dietary treatments (p>0.05). Daily excretion of creatinine was not affected by dietary treatments (p<0.05), with an average value of $0.334{\times}0.005$ mmol/kg $BW^{0.75}$. A linear correlation was found between total PD excretion and PDC index ($R^2$ = 0.93). Concentrations of creatinine and PDC index in spot urine were unaffected by sampling time (p>0.05) and a good correlation was found between the PDC index (average value of three times) of spot urine and daily excretion of PD ($R^2$ = 0.88). These results suggest that for animals fed ad libitum, the PDC index in spot urine is effective to predict daily excretion of PD. In order to improve the accuracy of the spot sampling technique, an appropriate lag phase between the time of feeding and sampling should be determined so that the sampling time can coincide with the peak concentration of PD in the urine.

• Korean Journal of Environmental Agriculture
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• v.36 no.3
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• pp.154-160
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• 2017

BACKGROUND:This study was carried out to establish an efficient sample preparation for the simultaneous determination of bisphenols (BPs) in river water samples using gas chromatography-mass spectrometry (GC-MS). Sample preparation was examined with conventional extraction methods, such as solid-phase extraction (SPE) and liquid-liquid extraction (LLE), and their efficiency was compared with validation results, including linearity of calibration curve, method detection limit (MDL), limit of quantification (LOQ), accuracy, and precision. METHODS AND RESULTS:The BPs (bisphenol A, BPA; bisphenol B, BPB; bisphenol C, BPC; bisphenol E, BPE; bisphenol F, BPF; bisphenol S, BPS) were analyzed using GC-MS. The range of MDLs by SPE and LLE methods was $0.0005{\sim}0.0234{\mu}g/L$ and $0.0037{\sim}0.2034{\mu}g/L$, and that of LOQs was $0.0015{\sim}0.0744{\mu}g/L$ and $0.0117{\sim}0.6477{\mu}g/L$, respectively. The calibration curve obtained from standard solution of $0.004{\sim}4.0{\mu}g/L$ (SPE) and $0.016{\sim}16{\mu}g/L$ (LLE) showed good linearity with $r^2$ value of 0.9969 over. Accuracy was 93.2~108% and 97.4~120%, and precision was 1.7~4.6% and 0.7~6.5%, respectively. The values of MDL and LOQ resulted from the SPE method were higher than those from the LLE method, particularly those values of BPA were highest among the BPs. Based on the results, the SPE method was applied to determine the BPs in river water samples. Water samples were collected from mainstream, tributary and sewage wastewater treatment plants (SWTPs) in the Yeongsan river basin. The concentration of BPB, BPC, BPE, BPF and BPS were not detected in all sites, whereas BPA was ranged $0.0095{\sim}0.2583{\mu}g/L$, which was $0.0166{\sim}0.0810{\mu}g/L$ for mainstreams, $0.0095{\sim}0.2583{\mu}g/L$ for tributaries, $0.0352{\sim}0.1217{\mu}g/L$ for SWTPs. CONCLUSION: From these results, the SPE method was very effective for the simultaneous determination of BPs in river water samples using GC-MS. We provided that it is a convenient, reliable and sensitive method enough to monitor and understand the fate of the BPs in aquatic ecosystems.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.2
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• pp.95-106
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• 1998

To reduce the environmental contamination and to utilize fly ash massively produced from the coal power plant every year, we synthesized the artificial zeolite using fly ash treated with alkaline, and then analyzed the mineralogical and morphological properties by X-ray, IR, and SEM. The amount of $NH_4{^+}$, $K^+$, and $H_2PO_4{^-}$ adsorbed by the fly ash and the artificial zeolite were determined with reaction time, amount of adsorbate used, ion concentrations. The results obtained from the pot experiments packed with the top soil, amended with granulated artificial zeolite which was made by treatment of 4% polyvinylalcohol, showed that CEC of the artificial zeolite was $257.7cmol^+kg^{-1}$, that was almost 36 times greater than that of fly ash. The ratio of $SiO_2/Al_2O_3$ decreased but the amount of Na increased. The physico-chemical properties analyzed by X-ray, IT, and SEM represented that the artificial zeolite synthesized had a similar morphological structure to that of the natural zeolite. The structures of the artificial zeolite had a significantly enlarged surface having a lot of pores, while the fly ash looked like spherical smooth shape with having not pores on the surface. Thus, the artificial zeolite was successfully synthesized. The results of adsorption isotherms of fly ash and artificial zeolite showed that the amount of $NH_4{^+}$, $K^+$, and $H_2PO_4{^-}$ adsorbed increased as the equilibrium concentration increased, while $NH_4{^+}$ was strongly adsorbed on the surface of fly ash and artificial zeolite than that of $K^+$. The most distinctive growth of Chinese cabbage was found from the top soil(NPK + soils + 20% of granulated artificial zeolite + 5% of compost). Therefore, we concluded that one of the most effective methods to effectively recycle a fly ash was to make the artificial zeolite as we did in this experiment.

• Horticultural Science & Technology
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• v.18 no.5
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• pp.612-620
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• 2000

In developing soil wetting agent using polyoxyethylene octylphenyl ether [$C_8H_{17}O(C_2H_4O)_{10}H$, POE], the effect of mixtures of POE and polyoxyethylene+polypropyleneoxide tridecylether (1:1, w/w, CM-1), polyoxyethylene+polypropyleneoxide tridecylether+propyleneglycol monomethylether (1:1:2, w/w/w, CM-2) or glycerin+ditridecyl phthalate (1:1, w/w, CM-3) on changes of concentration of POE, initial wetting, evaporative water loss, water infiltration, and changes of physical properties in root media were determined. The mixtures of POE and CM-1, 2, or 3 with zeolite or vermiculite as carrier had high concentrations of POE in root media during 6 elution times, but those after 6 times decreased rapidly indicating unstable elution of POE. The commercial AquaGro was more effective than the mixtures of POE+CM-1, 2, or 3 in water retention during 18 hours. Root media incorporated with POE+CM-2 held more water than any other treatments at 84 hours after watering, but the differences among treatments except control were not significant. In the treatments with zeolite as a carrier, POE+CM-3 had the greatest evaporative water loss followed by POE+CM-2, AquaGro, control, and POE+CM-2. In the treatments with vermiculite as a carrier, POE+CM-1 had the highest evaporative water loss followed by POE+CM-3, AquaGro, control, and POE+CM-2. In the effect of mixtures with zeolite as a carrier on infiltration of water into root media, the treatment of POE+CM-1 had the highest amount of water infiltrated followed by POE+CM-3, AquaGro, POE+CM-2, and control. In the effect of mixtures with vermiculite as a carrier on infiltration of water into root media, the treatment of POE+CM-3 had the highest amount of water infiltrated followed by AquaGro, POE+CM-1, POE+CM-2, and control.

• Journal of Embryo Transfer
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• v.19 no.2
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• pp.173-183
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• 2004

This study was carried out to examine the effects of epidermal growth factor (EGF) and the number of cumulus-oocyte complexes (COCs) on in vitro maturation (IVM) of porcine immature oocytes, and on subsequent in vitro fertilization (IVF) and development (IVD). COCs were collected from antral follicles of porcine ovaries collected from abattoir, and were maturated in modified NCSU-23 (mNCSU-23) with 10% pFF, 0.6 mM cysteine, 50 ${\mu}mM{\beta}-mercaptoethanol$, 1 mM dbcAMP, 10 IU/mL PMSG and 10 IU/mL hCG, which was supplemented with or without 10 ng/mL EGF and into which 50 or 15 COCs per droplet was put. Oocytes matured in vitro, were fertilized in vitro in modified Tris-buffered medium (mTBM) with the final motile sperm concentration of 1${\times}$105 sperm/mL, and subsequently putative embryos were developed in vitro in NCSU- 23. The results are as follows. 1.In the result of IVM, 10 ng/mL EGF supplement duplicated the percentage of C4 group of COCs(41% vs 81%). But the rate of germinal vesicle breakdown (GVBD) and of nuclear maturation were not significantly different between control and EGF supplemented, or between the number of COCs per culture droplet, and there was not a significant interaction between the two factors, either. 2. In the result of IVF, there was not significantly different between control and EGF supplemented, or between the number of COCs per culture droplet, or was not a significant interaction between the two factors, in the rate of sperm penetration, in the percentage of oocytes with male pronucleus (MPN), and in the rate of polyspermy. 3. In the result of IVD, there was not significantly different between control and EGF supplemented, or between the number of COCs per culture droplet in the percentage of cleaved oocytes. There was not significantly different between the number of COCs per culture droplet, but between control and EGF supplemented (p<0.01) in the percentage of blastocysts, the number of inner cell mass (ICM), trophectoderm (TC) and total cells. There was no significant interaction between the two factors anywhere. These results suggested that 10 ng/mL EGF supplement into mNCSU-23 for IVM was effective in the production of more as well as better blastocysts during IVD through increasing the number of cells in those.

• Journal of Periodontal and Implant Science
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• v.29 no.1
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• pp.1-14
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• 1999

Ursodeoxycholic acid(UDCA) is a hydrophilic gall bladder acid and has been used as a effective drug for liver disease related to in1munity. This drug inhibits secretions of IL-2, IL-4, and $IFN-{\gamma}$ from T-cells and production of immunoglobulin from B-cells. Also it has been reported that UDCA inhibits production of IL-1 related to the progression of periodontal disease and activation of collagenases. The purpose of the present study was to elucidate the effects of UDCA on inhibition of periodontal disease progression using clinical, microbiological and histometrical parameters. Twelve pure bred, 16 month-old-beagle dogs were used in the study. After ligature-induced periodontal diseases were formed, experimental drugs were applied twice a day and then the results of clinical, microbiological, and histometrical parameters were measured at baselie(initiation of experiment) , 4weeks and 8weeks. The gel with UDCA(concentration 0.5%, 5% 3 dogs in each) was applied to experimental group, chlorhexidine to positive control group(3dogs) and the gel without UDCA(base) to negative control group. After induction of general anesthesia, the maxillary 2nd, 3rd premolars and 1st molar and the mandibular 2nd, 3rd, 4th premolars and 1st molar were ligated in one side selected randomly and were not ligated in the opposite side. The plaque index(PI), gingival index(GI), pocket depth(PD) and gingival crevicular fluid(GCF) volum were measured clinically. The PI and GI were measured at 3 buccal points of all experimental teeth and the GCF was measured only at the 3rd premolar in the maxilla and the 4th premolar in the mandible. In the microbiological study, the samples extracted from the 3rd premolar of the maxilla and the 4th premolar of the mandible at the center of buccal surface were analyzed aerobics, anaerobics and Streptococcus colony forming units, After clinical and microbiological examination at 8weeks, the dogs were sacrificed by carotid artery perfusion. The samples were fixed and sectioned including interproximal area, and the distance from cementoenamel junction(CEJ) to alveolar crest was measured. The results were that PI, GI and PD increased until 4 weeks and decreased at 8 weeks in three groups but the differences between all the groups were not significant. The 0.5% UDCA in non-ligated group showed remarkable decrease of GCF. The experimental group applied 5% UDCA decreased the number of aerobics and anaerobics. The distance from CEJ to alveolar crest was greater in the negative control group on both ligated and non-ligated sides, but the differences were not significant stastically.

• Journal of Life Science
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• v.18 no.2
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• pp.167-174
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• 2008

Antimicrobial activity of Zanthoxylum piperitum was investigated against Streptococcus mutans that causes dental caries. Although the methylene chloride extract of seeds exhibited higher antimicrobial activity than other organic solvent extracts, including methanol, ethyl acetate, and hexane extracts of pericarps or seeds of Z. piperitum, essential oils prepared from both seeds and pericarps possessed more potent inhibitory activity than the methylene chloride extract of seeds. The minimal inhibitory concentrations (MICs) of the essential oils of seeds and pericarps were 0.3 mg/ml and 4.0 mg/ml against S. mutans, respectively. When the seed essential oil was further separated into seven fractions (CS-SD-A${\sim}$CS-SD-G) by thin layer chromatography (TLC), all fractions exhibited lower antimicrobial activity than the essential oil. To understand the antimicrobial ingredients of Z. piperitum, seeds the gas chromatography-mass spectrometry (GC-MS) data of the methylene chloride extract of seeds was compared with those of the seed essential oil (CS-SD). Whereas the methylene chloride extract of seeds contained carvacrol (0.24%), ${\beta}$-caryophyllene (1.72%), and ${\alpha}$-humulene (0.88%), which were previously known to inhibit growth of S. mutans, the seed essential oil contained sabinene (1.57%), linalool (1.55%), citronellal (13.67%), terpinene-4-ol (0.45%), citronellol (3.69%), geraniol (0.9%), linalyl acetate (1.35%), ${\beta}$-caryophyllene (1.35%), ${\alpha}$-humulene (0.78%), and ${\delta}$-cadinene (0.67%) in this regard. These results indicate that Z. piperitum seeds possess various inhibitory substances against S. mutans, and an effective method to isolate the active ingredients from the seeds is to prepare the essential oil. These results also suggest that the essential oil of Z. piperitium seeds may be applicable to preventing dental caries.

• Food Science and Preservation
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• v.9 no.3
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• pp.321-326
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• 2002

Paeksulkis(Korean rice cake) containing 0-0.5% chitosan were prepared for test the quality of microbiological, mechanical and sensory characteristics. The pH of Paeksulkis was 5.65 without chitosan and that was about 7.0 with 0.05-0.5% level of chitosan. In Hunter's color values of Paeksulkis of control, the lightness(L) was 84.28, redness(a) was -1.56 and yellowness(b) was 7.68. The lightness(L), redness(a) and yellowness(b) were increased with increasing concentration of chitosan in Paeksulkis. In mechanical characteristics of Paeksulkis, cohesiveness and springiness were the highest in control group while strength, hardness, gumminess and brittlenes were higher in chitosan added group than control group. In sensory evaluation of Pasksulkis, control group obtained the highest score in color, texture, after swallowing and overall quality(p<0.05) but chitosan added group obtained higher scores in moisture than control(p<0.05). Total bacterial counts(TBC) of Paeksulgis immediately before storage were 4.2∼9.2$\times$10$^2$CFU/g and those of control increased for 2 weeks, reached at 7.4$\times$10$\^$5/ CFU/g and then decreased about 1 log cycle for 2 weeks during storage at 5$\^{C}$. TBC of Paeksulgis added 0.3∼0.5% of chitosan were 2 log cycles lower than that of control at the end of storage at 5$\^{C}$. TBC of Pasksulgis control increased to 10$\^$8/ CFU/g during storage at 20$\^{C}$ but that of 0.5% chitosan added group was 1 log cycle lower than control at the end of storage. Shelf-life extension of Paeksulkis by chitosan was more effective during storage at 5$\^{C}$ than at 20$\^{C}$.