• Journal of Pharmaceutical Investigation
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• 제31권3호
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• pp.185-190
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• 2001

Human papillomavirus (HPV) infection is known to cause cervical cancers. Human papillomavirus-like particles (VLP) have been studied as preventive vaccines of cervical cancers. To develop VLP as a therapeutic gene carrier, we studied the method to encapsulate cytokine genes in virus-like particles. HPV type 16 capsid L1 genes were amplified by polymerase chain reaction and cloned into T vector. L1 gene was then inserted into baculovirus transfer vector. The clone of baculovirus encoding L1 gene was isolated and used to express L1 protein in Sf 21 insect cells. VLP were purified by CsCl density gradient and ultracentrifugation. VLP were disassembled to capsomer units by treatment of a reducing agent. Given that interleukin-2 (IL-2) genes have been used in anticancer gene therapy and as a molecular adjuvant, IL-2 cytokine plasmids were chosen as a model gene. IL-2 plasmids were incubated with the disassembled capsomer suspension. To reassemble the particles, the mixture of capsomers and cytokine plasmids was dialyzed. The disassembly and reassembly of VLP were confirmed by transmission electron microscopy. The entrapment of cytokine plasmids in reassembled VLP was tested by the stability of plasmids against DNase I. After treatment of reassembled virus-like particles with DNase I, discrete IL-2 DNA band was observed. Our results indicate that IL-2 cytokine plasmid (3.5 kb size) can be encapsulated in the virus-like particles, suggesting the potential of VLP as a gene delivery system. Moreover, VLP containing the adjuvant cytokine plasmids might function as more effective subunit vaccines.

• 대한전기학회논문지:전력기술부문A
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• 제53권11호
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• pp.596-603
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• 2004

This paper proposes an expert system based on the pattern recognition method which can enhance the accuracy and effectiveness of real-time bus reconfiguration strategy for the transfer of faulted load when a main transformer fault occurs in the automated substation. The minimum distance classification method is adopted as the pattern recognition method of expert system. The training pattern set is designed MTr by MTr to minimize the searching time for target load pattern which is similar to the real-time load pattern. But the control pattern set, which is required to determine the corresponding bus reconfiguration strategy to these trained load pattern set is designed as one table by considering the efficiency of knowledge base design because its size is small. The training load pattern generator based on load level and the training load pattern generator based on load profile are designed, which are can reduce the size of each training pattern set from max L/sup (m+f)/ to the size of effective level. Here, L is the number of load level, m and f are the number of main transformers and the number of feeders. The one reduces the number of trained load pattern by setting the sawmiller patterns to a same pattern, the other reduces by considering only load pattern while the given period. And control pattern generator based on exhaustive search method with breadth-limit is designed, which generates the corresponding bus reconfiguration strategy to these trained load pattern set. The inference engine of the expert system and the substation database and knowledge base is implemented in MFC function of Visual C++ Finally, the performance and effectiveness of the proposed expert system is verified by comparing the best-first search solution and pattern recognition solution based on diversity event simulations for typical distribution substation.

• 대한전기학회논문지:전력기술부문A
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• 제49권5호
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• pp.226-232
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• 2000

This paper presents application results of the augmented matrix eigen-sensitivity theories to TCSC siting problem for damping the inter-area low frequency oscillation in the large KEPCO system. First and second-order eigen-sensitivities of the inter-area low frequency oscillation in the large KEPCO system. First and second-order eigen-sensitivities of the inter-area mode are computed fro changes in susceptance of the transmission lines. The lines having high sensitivity are chosen as the initial candidates for installing TCSC. Then for each of the chosen candidates, Bodeplot of the transfer function with line susceptance as the input and the bus voltage at one side of the line as the output is computed. Using the Bode plots, the lines having any zeros near the inter-area mode are screened out since design of TCSC controller is very difficult in such a case. The $H_{\infty}$ TCSC controller installed at any finally chosen candidate is found to be effective in damping the inter-area oscillation, and the proposed TCSC siting algorithm is proved to be valid. Design of $H_{\infty}$ controller is described in Part IIof this paper.

• Journal of Advanced Marine Engineering and Technology
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• 제3권1호
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• pp.19-31
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• 1979

Recently the adaptive control system, which keeps the control system always optimal by adjusting the control parameters automatically according to the variations of the plant parameters, have become very important in the field of control engineering. The adaptive control systems are usally composed of the plant identification, the decision of the optimal control parameters, and the adjustment of the control parameters. This paper deals with a method of the adaptive control system when PI or PID controller is used in the feed back control system. Its controlled object (the plant) is assumed to be described by the transfer function of $\frac{ke^{-LS}}{1+TS}$ where k, T and L are steady state gain, time constant and pure dead time respectively, and their values are variable in accordance with the change of environmental circumstance. It has been known that a pseudo-random binary signal is quite effective for the measurement of an impulse response of a plant. In adaptive control systems, however, the impulse response itself is not appropriate to determine the control parameters. In this paper, the authors propose a method to estimate directly the parameters of the plant k, T and L by means of the correlation technique using 3 level M-sequence signal as a test signal. The authors also propose a method to determine the optimal parameters of the PI or PID controller in the sense of minimizing the square integral of the control error in the feed back control system, and the values of the optimal parameters are computed numerically for various values of T and L, and the results are examined and compared with those of the conventional methods. Finally the above-mentioned two methods are combined and an algorithm to struct an adaptive control system is suggested. The experiments for the indicial responses by means of both the model of the temperature control system using SCR actuater and the analog simulations have shown good results as expected, and the effectiveness of the proposed method is verified. The M-sequence generator and the time delay circuit, which are manufactured for the experiments, are operated in quite a good condition.

• 드라이브 ㆍ 컨트롤
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• 제13권3호
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• pp.24-31
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• 2016

The performance characteristics of a dynamic control system are evaluated according to the transient and steady-state responses. The transient performance is the controllability of the output for the tracking of the reference or the ability to reduce or reject the effects of unwanted disturbances; alternatively, the steady-state performance is represented by the magnitude of the control error at the steady state. As the effects of the two performances on each other are reciprocal, a controller design that shows a zero steady-state error for the ramp input is uncommon because of the challenge regarding the achievement of an acceptable transient response. This paper proposes a PI+double-integral controller for the elimination of the steady-state error for the ramp input while a sound transient performance is maintained. The control-gain design procedure is described by the second-order response for the step input and the response of the error dynamics for the ramp input. The PI+double-integral controller is designed for the first-order transfer function that is derived from a system identification with the open-loop experiment data of the dc-motor. The simple structure of the proposed controller enables the adoption of a low-end microcontroller for the implementation of a real-time control. The experiment results show that the control performance is as effective as that of the simulation analysis for the operating point of linear system; furthermore, the PI+double-integral controller can be conveniently applied to the control system, which is desirable for the improvement of the steady-state error.

• 대한기계학회논문집
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• 제17권1호
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• pp.101-111
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• 1993

본 연구에서는 제안된 뉴로-퍼지 제어기를 사용하여 유압 서보 시스템을 제어 하고 학습하기 위한 구조로써 유압 서보 시스템의 모델링을 위한 추가적인 노력이 필 요없는 feedback error learning 구조물 채택하였다. 학습 과정에서 필요한 유압 서 보 시스템의 입-출력 사이의 감도(sensitivity)의 효과는 학습 계수에 포함된다. 이 러한 형태의 제어기가 유압 서보 시스템 제어에 유용하게 적용될 수 있다는 것을 보이 기 위해서 불확실성과 높은 비선형성 뿐만아니라 외란의 영향을 받는 유압 서보 시스 템을 대상으로 시뮬레이션을 수행했다. 시뮬레이션 결과에 의하면 제안된 뉴로-퍼지 제어기는 수학적인 모델을 기초로한 기존의 제어 알고리즘에 비해 쉽게 구성할 수 있 고 높은 정밀도, 빠른 학습 속도를 얻을 수 있는 장점을 가지고 있음을 알 수 있다.

• Bulletin of the Korean Chemical Society
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• 제34권2호
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• pp.447-452
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• 2013

In this study we report a new method for the synthesis of a silica monolithic column bed with bimodal pores (throughpores and mesopores). The template induced synthesis method was used to direct bimodal pores simultaneously instead of the usual post base-treating method. Block polymer Pluronic F127 was chosen as a dual-function template to form hierarchically porous silica monolith with both macropores and mesopores. This is a simplification of the method of monolithic column preparation. Poly(ethylene glycol) was used as a partial substitute for F127 can effectively prevent shrinkage during the monolith aging process without losing much surface area (944 $m^2/g$ to 807 $m^2/g$). More importantly, the resultant material showed a much narrower mesopore size (centered at 6 nm) distribution than that made using only F127 as the template reagent, which helps the mass transfer process. The solvent washing method was used to remove the remaining organic template, and it was proved to be effective enough. The new synthesis method makes the fabrication of the silica monolithic column (especially capillary column) much easier. All the structure parameters indicate that monolith PFA05 prepared by the above method is a good material for separation, with the merits of much higher surface area than usual commercial HPLC silica particles, suitable mesopore volume, narrow mesopore size distribution, low shrinkage and it is easily prepared.

• Earthquakes and Structures
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• 제12권4호
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• pp.425-436
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• 2017

Vibration control using a tuned mass damper (TMD) is an effective technique that has been verified using analytical methods and experiments. It has been applied in mechanical, automotive, and structural applications. However, the damping of a TMD cannot be adjusted in real time. An excessive mass damper stroke may be introduced when the mass damper is subjected to a seismic excitation whose frequency content is within its operation range. The semi-active tuned mass damper (SATMD) has been proposed to solve this problem. The parameters of an SATMD can be adjusted in real time based on the measured structural responses and an appropriate control law. In this study, a stiffness-controllable TMD, called a leverage-type stiffness-controllable mass damper (LSCMD), is proposed and fabricated to verify its feasibility. The LSCMD contains a simple leverage mechanism and its stiffness can be altered by adjusting the pivot position. To determine the pivot position of the LSCMD in real time, a discrete-time direct output-feedback active control law that considers delay time is implemented. Moreover, an identification test for the transfer function of the pivot driving and control systems is proposed. The identification results demonstrate the target displacement can be achieved by the pivot displacement in 0-2 Hz range and the control delay time is about 0.1 s. A shaking-table test has been conducted to verify the theory and feasibility of the LSCMD. The comparisons of experimental and theoretical results of the LSCMD system show good consistency. It is shown that dynamic behavior of the LSCMD can be simulated correctly by the theoretical model and that the stiffness can be properly adjusted by the pivot position. Comparisons of experimental results of the LSCMD and passive TMD show the LSCMD with less demand on the mass damper stroke than that for the passive TMD.

• Asia pacific journal of information systems
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• 제18권4호
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• pp.57-81
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• 2008

Knowledge is an essential strategic weapon for sustaining competitive advantage and is the key determinant for organizational growth. When knowledge is shared and disseminated throughout the organization, it increases an organization's value by providing the ability to respond to new and unusual situations. The growing importance of knowledge as a critical resource has forced executives to pay attention to their organizational knowledge. Organizations are increasingly undertaking knowledge management initiatives and making significant investments. Knowledge sourcing is considered as the first important step in effective knowledge management. Most firms continue to make an effort to realize the benefits of knowledge management by using various knowledge sources effectively. Appropriate knowledge sourcing strategies enable organizations to create, acquire, and access knowledge in a timely manner by reducing search and transfer costs, which result in better firm performance. In response, the knowledge management literature has devoted substantial attention to the analysis of knowledge sourcing strategies. Many studies have categorized knowledge sourcing strategies into intemal- and external-oriented. Internal-oriented sourcing strategy attempts to increase firm performance by integrating knowledge within the boundary of the firm. On the contrary, external-oriented strategy attempts to bring knowledge in from outside sources via either acquisition or imitation, and then to transfer that knowledge across to the organization. However, the extant literature on knowledge sourcing strategies focuses primarily on large organizations. Although many studies have clearly highlighted major differences between large and small firms and the need to adopt different strategies for different firm sizes, scant attention has been given to analyzing how knowledge sourcing strategies affect firm performance in small firms and what are the differences between small and large firms in the patterns of knowledge sourcing strategies adoption. This study attempts to advance the current literature by examining the impact of knowledge sourcing strategies on small firm performance from a holistic perspective. By drawing on knowledge based theory from organization science and complementarity theory from the economics literature, this paper is motivated by the following questions: (1) what are the adoption patterns of different knowledge sourcing strategies in small firms (i,e., what sourcing strategies should be adopted and which sourcing strategies work well together in small firms)?; and (2) what are the performance implications of these adoption patterns? In order to answer the questions, this study developed three hypotheses. First hypothesis based on knowledge based theory is that internal-oriented knowledge sourcing is positively associated with small firm performance. Second hypothesis developed on the basis of knowledge based theory is that external-oriented knowledge sourcing is positively associated with small firm performance. The third one based on complementarity theory is that pursuing both internal- and external-oriented knowledge sourcing simultaneously is negatively or less positively associated with small firm performance. As a sampling frame, 700 firms were identified from the Annual Corporation Report in Korea. Survey questionnaires were mailed to owners or executives who were most erudite about the firm s knowledge sourcing strategies and performance. A total of 188 companies replied, yielding a response rate of 26.8%. Due to incomplete data, 12 responses were eliminated, leaving 176 responses for the final analysis. Since all independent variables were measured using continuous variables, supermodularity function was used to test the hypotheses based on the cross partial derivative of payoff function. The results indicated no significant impact of internal-oriented sourcing strategies while positive impact of external-oriented sourcing strategy on small firm performance. This intriguing result could be explained on the basis of various resource and capital constraints of small firms. Small firms typically have restricted financial and human resources. They do not have enough assets to always develop knowledge internally. Another possible explanation is competency traps or core rigidities. Building up a knowledge base based on internal knowledge creates core competences, but at the same time, excessive internal focused knowledge exploration leads to behaviors blind to other knowledge. Interestingly, this study found that Internal- and external-oriented knowledge sourcing strategies had a substitutive relationship, which was inconsistent with previous studies that suggested complementary relationship between them. This result might be explained using organizational identification theory. Internal organizational members may perceive external knowledge as a threat, and tend to ignore knowledge from external sources because they prefer to maintain their own knowledge, legitimacy, and homogeneous attitudes. Therefore, integrating knowledge from internal and external sources might not be effective, resulting in failure of improvements of firm performance. Another possible explanation is small firms resource and capital constraints and lack of management expertise and absorptive capacity. Although the integration of different knowledge sources is critical, high levels of knowledge sourcing in many areas are quite expensive and so are often unrealistic for small enterprises. This study provides several implications for research as well as practice. First this study extends the existing knowledge by examining the substitutability (and complementarity) of knowledge sourcing strategies. Most prior studies have tended to investigate the independent effects of these strategies on performance without considering their combined impacts. Furthermore, this study tests complementarity based on the productivity approach that has been considered as a definitive test method for complementarity. Second, this study sheds new light on knowledge management research by identifying the relationship between knowledge sourcing strategies and small firm performance. Most current literature has insisted complementary relationship between knowledge sourcing strategies on the basis of data from large firms. Contrary to the conventional wisdom, this study identifies substitutive relationship between knowledge sourcing strategies using data from small firms. Third, implications for practice highlight that managers of small firms should focus on knowledge sourcing from external-oriented strategies. Moreover, adoption of both sourcing strategies simultaneousiy impedes small firm performance.

• 한국콘텐츠학회논문지
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• 제11권11호
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• pp.102-110
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• 2011

본 논문은 플레이어와 게임간의 상호작용의 의미를 조이스틱, 키보드와 같이 조작적 상호작용 뿐만 아니라 플레이어가 게임을 플레이하면서 보고 듣고 이해하는 표상적 상호작용을 포함하여 접근하고 있다. 기존게임 내에 삽입되는 동영상(cut-scene)은 주로 게임의 배경스토리를 전달하는 오프닝 영상과, 플레이어 행동의 결과를 재생(replay)하거나 플레이어의 상태를 부가적으로 표현하는 수동적인 의미의 영상으로 인식되어 왔다. 그러나 게임연출 측면에서 대표적인 비디오게임 '언차티드2'의 인터랙션 무비에 대한 플레이어의 행동과 그 결과를 조사해 보면 크게 3가지의 기능(상황연출, 선택적 행동유발, 정보전달)으로 인터랙션 무비를 분류할 수 있었다. 그 결과, 인터랙션 무비는 게임플레이를 위한 부가적인 요소가 아닌 인터페이스로써의 기능을 수행하고 있다고 판단된다. 인터랙션 무비의 효과적인 적용은 플레이어 관점에서 인지하고 주인공 캐릭터 관점에서 플레이하는 과정을 유기적으로 연결시킬 수 있어 게임의 스토리 전달을 극대화하는 직접적인 수단으로 활용될 수 있다.