• Nutrition Research and Practice
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• 제13권6호
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• pp.480-487
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• 2019

BACKGROUND/OBJECTIVES: Osteoarthritis (OA) is a major public health issue in Japan and other countries, and foods that prevent or treat OA are in strong demand. Proteins and peptides in chicken meat and bones are known for being rich in functional and nutritional ingredients for the improvement of osteoporosis. We speculated that chicken legs, a food consumed in many regions of the world, may also contain such ingredients. In this study, we aim to (i) evaluate the effect of chicken leg extract (CLE) on the promotion of cartilage matrix production and (ii) identify the active ingredient in CLE that contributes to this function. MATERIALS/METHODS: Artificial CLE digest was prepared, and the acid mucopolysaccharide production-promoting activity of the CLE digest was evaluated by alcian blue staining of ATDC5 cells. CLE was orally administered to rabbits with burr holes in the knee joint of the femur, and the degree of regeneration of cartilage matrix was evaluated. Furthermore, we investigated orally administered CLE-derived peptides in human plasma using LC-MS. From measuring the acid mucopolysaccharide production-promotion activity of these peptides, a molecule considered to be an active ingredient in the CLE digest was identified. RESULTS: CLE digest promoted acid mucopolysaccharide production and facilitated regeneration of cartilage matrix in in vitro and in vivo experiments. Four peptides including phenylalanyl-hydroxyproline (Phe-Hyp) were detected as CLE-derived peptides in human plasma. The effect of CLE was inferred to be due to Phe-Hyp, which was confirmed to be present in the CLE digest. CONCLUSIONS: It was shown that CLE stimulated the production of articular cartilage matrix both in vitro and in vivo, and that CLE could be an effective food for preventing or treating OA. Furthermore, only Phe-Hyp was confirmed as the active compound in the CLE digest, suggesting that the activity of CLE was due to Phe-Hyp.

• 약학회지
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• 제57권1호
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• pp.1-7
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• 2013

Induction of effective and increased levels of antibody production may be major points in vaccine development. This is especially the case when the antigenic sources are carbohydrates. Thus, in our Lab various types of formulations such as liposomal and conjugate vaccines have been researched. However, the fastidious formulation process and high costs are a problem. For this reason, there is currently a focus on utilizing immunoadjuvants. In this present study, we tested whether ginsenosides Re (a panaxdiol) and Rg1 (a panaxtriol) from Panax ginseng have immunoadjuvant activity against the cell wall of Candida albicans (CACW). The resulting data showed that Rd and Rg1 caused LPS-treated B lymphocytes to be proliferative. Rd had greater proliferation activity than that of Rg1. In the murine model of antibody production, CACW combined with Rd [CACW/Rd/IFA] or Rg1 [CACW/Rg1/IFA] increased the production of antibodies specific to C. albicans when compared to the antibody production by [CACW/IFA]-induction, which was used as a negative control (P<0.05). In the case of [CFA/Rd/IFA], the antibody production was almost twice as that of the CFA. In addition, formulations containing either had a prolonged antibody inducing activity as compared to the CFA formula. In conclusion, Rd and Rg1 have an immunologic activity, and yet Rd can be a better candidate than Rg1 for a new immunoadjuvant.

• 한국융합학회논문지
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• 제12권10호
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• pp.71-79
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• 2021

제조업 분야에서는 경쟁력 강화를 위하여 다품종 생산으로의 전환이 이루어지고 있으며, 하이브리드 제조기술이 증가하고 있다. 특히 제조실행시스템과 ERP 등의 도입을 통하여 생산 품질 향상에 많은 노력을 기울이고 있기에, 신속하고 효과적인 관리를 위한 MES 운영이 필요하다. MES는 제품 생산과 관련된 모든 단계를 관리하면서, 생산 활동에 비효율적인 부분은 개선하고, 공정 변경의 경우에 시스템에 변경 사항을 반영해야 한다. 그러나 대부분의 MES는 시스템 관리자를 통하여 수동적이고, 반복적으로 비효율적인 작업을 계속 하고 있는 상황이다. 본 논문에서는 생산과 관련된 요구사항을 시스템 관리자가 특정 라인의 장비에 대한 참조 정보를, 다른 라인의 동일한 장비에도 일괄 적용할 수 있는 모델을 제안한다. 제안 모델을 통하여 참조 정보의 일괄 적용과 선택 적용의 구분으로 생산 라인에 대한 유연한 대처가 가능할 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• 제29권8호
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• pp.1288-1298
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• 2019

Bacterial ATP synthases drive ATP synthesis by a rotary mechanism, and play a vital role in physiology and cell metabolism. Corynebacterium glutamicum is well known as an industrial workhorse for amino acid production, and its ATP synthase operon contains eight structural genes and two adjacent genes, cg1360 and cg1361. So far, the physiological functions of Cg1360 (GenBank CAF19908) and Cg1361 (GenBank CAF19909) remain unclear. Here, we showed that Cg1360 was a hydrophobic protein with four transmembrane helices (TMHs), while no TMH was found in Cg1361. Deletion of cg1360, but not cg1361, led to significantly reduced cell growth using glucose and acetic acid as carbon sources, reduced F1 portions in the membrane, reduced ATP-driven proton-pumping activity and ATPase activity, suggesting that Cg1360 plays an important role in ATP synthase function. The intracellular ATP concentration in the ${\Delta}cg1360$ mutant was decreased to 72% of the wild type, while the NADH and NADPH levels in the ${\Delta}cg1360$ mutant were increased by 29% and 26%, respectively. However, the ${\Delta}cg1361$ mutant exhibited comparable intracellular ATP, NADH and NADPH levels with the wild-type strain. Moreover, the effect of cg1360 deletion on L-valine production was examined in the L-valine-producing V-10 strain. The final production of L-valine in the $V-10-{\Delta}cg1360$ mutant reached $9.2{\pm}0.3g/l$ in shake flasks, which was 14% higher than that of the V-10 strain. Thus, Cg1360 can be used as an effective engineering target by altering energy metabolism for the enhancement of amino acid production in C. glutamicum.

• Journal of Microbiology and Biotechnology
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• 제29권5호
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• pp.776-784
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• 2019

Polyhydroxybutyrate (PHB), the most well-known polyhydroxyalkanoate, is a bio-based, biodegradable polymer that has the potential to replace petroleum-based plastics. Lignocellulose hydrolysate, a non-edible resource, is a promising substrate for the sustainable, fermentative production of PHB. However, its application is limited by the generation of inhibitors during the pretreatment processes. In this study, we investigated the feasibility of PHB production in E. coli in the presence of inhibitors found in lignocellulose hydrolysates. Our results show that the introduction of PHB synthetic genes (bktB, phaB, and phaC from Ralstonia eutropha H16) improved cell growth in the presence of the inhibitors such as furfural, 4-hydroxybenzaldehyde, and vanillin, suggesting that PHB synthetic genes confer resistance to these inhibitors. In addition, increased PHB production was observed in the presence of furfural as opposed to the absence of furfural, suggesting that this compound could be used to stimulate PHB production. Our findings indicate that PHB production using lignocellulose hydrolysates in recombinant E. coli could be an innovative strategy for cost-effective PHB production, and PHB could be a good target product from lignocellulose hydrolysates, especially glucose.

• 융합정보논문지
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• 제9권4호
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• pp.8-13
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• 2019

본 논문은 지역의 문화콘텐츠의 제작기술과 인프라를 기반으로 스토리텔링 중심의 캐릭터 브랜드 상품의 부재로 인하여 지역 문화자원의 재구성 및 상품화가 필요하다고 판단되어 전통적 문화소재인 캐릭터를 발굴하여 문화산업 기념품으로 개발하였다. 스토리텔링 기반의 캐릭터 상품 개발에 대한 차별화 전략을 통해 발전된 사업기반을 확립할 수 있는 토대가 되어, 다양한 콘텐츠 제작의 밑거름과 더불어 발전된 도예 상품들의 공급 확대에 기여할 것으로 보여 진다. 또한, 수작업 기반의 제작으로 인해 소량 생산만 가능하고 제작기간이 길어지는 반면 3D 프로그램 및 3D 프린팅을 통한 목업 제작으로 석고 틀 제작 기간이 단축되었으며, 대량생산의 발판이 마련되어 기존의 전통적인 도자기 인형에서 벗어나 다양하고 풍부한 도자기 제품 개발에 효과가 있었다.

• 인삼문화
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• 제4권
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• pp.1-12
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• 2022

Ginseng and ginseng products are distributed in approximately 190 countries around the world. The size of the ginseng market varies by country and there are no accurate statistics on production and distribution amounts per country. Therefore, it is difficult to make predictions about the global ginseng market. Governments and ginseng trading companies are in need of comprehensive data that shows the current status of the ginseng market to help them establish effective import, export, and sales and marketing policies. To addressthis need, this study examines the approximate size of the world ginseng market based on estimates of recent quantities of ginseng distributed in specific country as well as production by major ginseng producing countries. In 2018, global ginseng production was about 86,223 tons based on fresh ginseng. China produced 50,164 tons, South Korea 23,265 tons, Canada 11,367 tons, the US 1,285 tons, Japan 30 tons, and other countries a combined 112 tons. The value of global ginseng production is estimated to be approximately $5,900 million, with $2,870 million (48.6%) in China, $2,489 million (42.2%) in South Korea, $478 million (8.1%) in Canada, $54 million (0.9%) in the USA, $4 million (0.1%) in Japan, and $5 million (0.1%) in other countries. The value of ginseng products consumed for the last five yearsin South Korea was $1,162 million in 2014, $1,280 million in 2015, $1,548 million in 2016, $1,638 million in 2017, and $1,762 million in 2018, showing that the market has been increasing in recent years. In particular, the Korea Ginseng Corporation (KGC), the biggest global ginseng company in South Korea, recorded sales of $1,207 million in 2018. This represents about 69% of the South Korean ginseng market, and about 20% of global production. Since interest in alternative medicine and health food among consumers is increasing globally, the market for ginseng is expected to expand into the future.

• Journal of the Korean Wood Science and Technology
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• 제30권1호
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• pp.72-78
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• 2002

본 연구에서는 검은 비늘버섯을 유용한 식용버섯으로 개발하기 위하여 톱밥 및 다양한 농산부산물을 기질로 하여 버섯재배를 실시하였으며, 기질의 혼합비가 버섯재배 및 생산량에 미치는 영향을 구명하고자 하였다. 톱밥을 이용한 버섯재배에서 수종별로는 미루나무가 795 g으로서 가장 많은 버섯이 생산되었으며, 아까시나무가 중간정도, 상수리나무가 3수종 가운데 가장 적은 양이 생산되었다. 3수종의 톱밥을 30% 및 50% 비율로 혼합한 배지에서 아까시나무톱밥의 혼합으로 많은 버섯 생산을 결과하였는데, 아까시나무톱밥 70%+미류나무톱밥 30%가 265 g의 버섯을, 아까시나무톱밥 50%+미류나무톱밥 50% 조합에서는 228 g의 높은 버섯생산량을 나타냈는데 대하여, 상수리나무톱밥이 혼합되면 버섯생산량이 낮아졌다. 이러한 결과로부터 톱밥 혼합 배지에서 아까시나무는 부재료로서 매우 가치가 있는 것으로 나타났다. 모든 농산부산물의 경우에도 버섯의 증수효과를 결과하였다. 이러한 결과로부터 검은비늘버섯의 재배에 톱밥, 특히 아까시나무 및 농산부산물을 이용할 수 있음이 밝혀졌다.

• 한국컴퓨터정보학회논문지
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• 제29권5호
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• pp.31-38
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• 2024

본 논문에서는 생성형 AI 기반의 광고 이미지 자동 제작 서비스를 제공하는 ASAP(AI-driven Service for Advertisement Production) 애플리케이션을 제안한다. 이 애플리케이션은 GPT-3.5 Turbo Instruct를 이용하여 사용자가 입력한 키워드에 적합한 배경 분위기와 홍보 문구를 생성한다. 이를 입력으로 하여 배경 이미지와 텍스트 이미지를 생성하기 위해 OpenAI사의 DALL·E 3 모델과 Stability AI사의 SDXL 모델을 활용한다. 추가적으로 OCR 기술을 활용하여 텍스트 이미지의 정확도를 높이고, 생성된 출력물들을 모두 합성하여 최종적인 광고를 제작한다. 또한 PILLOW, OpenCV 라이브러리의 텍스트 박스를 이용하여 전화번호, 영업시간 등 세부 사항을 홍보물의 가장자리에 삽입할 수 있도록 구현한다. 본 애플리케이션은 광고 제작에 어려움이 많은 소상공인들에게 광고를 쉽고 편리하게 제작할 수 있고, 광고 제작 비용을 절감할 수 있는 효과를 제공한다.

• Journal of Forest and Environmental Science
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• 제40권3호
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• pp.259-264
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• 2024

Dalbergia sissoo and Dalbergia latifolia are the two most important timber wood species that are indigenous to the Indo-Pak subcontinent producing very high-quality timber. Its wood is used to produce high-quality furniture. Due to overexploitation and dieback disease, their production is seriously hampered and threatens their genetic diversity. Several ecotypes are tolerant to dieback thus offering an opportunity to develop a superior hybrid for the establishment of plantations for hybrid seed production. Hybrid evaluation can only be done by using DNA markers such as SSR markers. Cross-specific amplification of SSR markers is a cost-effective way of producing DNA markers for species lacking genetic information. Here, we report for the first-time successful cross-amplification of SSR markers in Dalbergia latifolia and added new SSR markers in D. sissoo. Cross-species amplification resulted in 13 successful SSR markers in D. latifolia and an addition of 14 markers in D. sissoo of expected sizes. Six SSR markers were further selected randomly to validate the breeding behavior of both species. A diverse DNA profile of seed progenies matched to different pollen donors deviated from the same mother suggested cross-pollination is the most likely mechanism of seed production in D. sissoo and D. latifolia. However, the results must be validated by using a large sample size and through controlled pollinations. SSR markers thus developed will be useful in the conservation and development of superior hybrids for sustainable development and production of commercial populations in Dalbergia sissoo and D. latifolia.