• Journal of Ginseng Research
• /
• 제46권1호
• /
• pp.62-70
• /
• 2022

Background: Maternal Toxoplasma gondii (T. gondii) infection during pregnancy has been associated with various mental illnesses in the offspring. Ginsenoside Rh2 (GRh2) is a major bioactive compound obtained from ginseng that has an anti-T. gondii effect and attenuates microglial activation through toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signaling pathway. GRh2 also alleviated tumor-associated or lipopolysaccharide-induced depression. However, the effects and potential mechanisms of GRh2 on depression-like behavior in mouse offspring caused by maternal T. gondii infection during pregnancy have not been investigated. Methods: We examined GRh2 effects on the depression-like behavior in mouse offspring, caused by maternal T. gondii infection during pregnancy, by measuring depression-like behaviors and assaying parameters at the neuronal and molecular level. Results: We showed that GRh2 significantly improved behavioral measures: sucrose consumption, forced swim time and tail suspended immobility time of their offspring. These corresponded with increased tissue concentrations of 5-hydroxytryptamine and dopamine, and attenuated indoleamine 2,3-dioxygenase or enhanced tyrosine hydroxylase expression in the prefrontal cortex. GRh2 ameliorated neuronal damage in the prefrontal cortex. Molecular docking results revealed that GRh2 binds strongly to both TLR4 and high mobility group box 1 (HMGB1). Conclusion: This study demonstrated that GRh2 ameliorated the depression-like behavior in mouse offspring of maternal T. gondii infection during pregnancy by attenuating the excessive activation of microglia and neuroinflammation through the HMGB1/TLR4/NF-κB signaling pathway. It suggests that GRh2 could be considered a potential therapy in preventing and treating psychiatric disorders in the offspring mice of mothers with prenatal exposure to T. gondii infection.

• 공학교육연구
• /
• 제15권5호
• /
• pp.3-7
• /
• 2012

Utsunomiya University started a 3-year project to develop an education program for postgraduate students to cultivate leadership spirits. The program is centered on a PBL in which each group of students is required to find an engineering or social problem themselves and start a project to solve the problem. Projects vary widely, from a straight product development to a summer program for children or local area development. Student groups are advised by senior engineers from industries. They give advice as to how a project should be managed and run, but will not give instructions. The PBL is compulsory for all students in the engineering master course students. The new education program also includes a series of lectures by engineers to show engineers' job, activities, and career in the industry. Internship programs are also provided, in which students will be given opportunities to actively apply their knowledge and skills to real engineering tasks offered by the industry. The students are expected to gain something more than just experiences.

• 산업융합연구
• /
• 제12권2호
• /
• pp.54-62
• /
• 2014

The significance of the research is to leverage on the policy makers and school education, and research in terms of human rights issues and for the improvement of multicultural education by seeking an effective training plan with the purpose of the present study it. The efforts to address human rights issues through real multicultural care center starts with the question of human rights education in multicultural education methodology that can protect the human rights of migrant workers, immigrant youth in a multicultural society such situation in Korea by. Accordingly we suggest that it is appropriate to human rights education in the moral multicultural care center for human rights education. Cant because the regular students on the basis of morality that respects the human rights of migrant workers, immigrant adolescents 'human rights' training done 'pursue human rights' migrant worker, immigrant youth in their own rights, as well as multicultural education is through morality The Seen to suggest ways through the development of multicultural education issues and the results of these studies.

• Journal of Preventive Medicine and Public Health
• /
• 제45권2호
• /
• pp.78-89
• /
• 2012

Objectives: Although vaccination rates have increased, problems still remain in the storage and handling of vaccines. This study focused on inspecting actual vaccine storage status and awareness, and comparing them before and after education was provided. Methods: In the primary inspection, a status survey checklist was completed by visual inspection. A questionnaire on the awareness of proper vaccine storage and handling was also administered to vaccine administrators in private medical institutions in 4 regions in Gyeongsangbuk-province. One-on-one education was then carried out, and our self-produced manual on safe vaccine storage and management methods was provided. In the secondary inspection, the investigators visited the same medical institutions and used the same questionnaire and checklist used during the primary inspection. The results before and after education were compared, by treating each appropriate answer as 1 point. Results: The average checklists score was 9.74 (out of 15 points), which increased significantly after education was provided (by 0.84, p<0.001). The participants demonstrated improved practices in recording storage temperatures(p=0.016), storing vaccines in the center of the refrigerator (p=0.004), storing vaccines with other medication and nonmedical items (p=0.031) after education. The average score calculated from the questionnaires was 10.48 (out of 14points), which increased after education (by 1.03, p<0.001). Conclusions: This study suggests that vaccine storage practices and awareness are inadequate, but can be partially improved by providing relevant education. Repetitive education and policy-making are required to store vaccines safely because one-off education and unenforced guidelines offer limited efficacy.

• Journal of Plant Biotechnology
• /
• 제4권3호
• /
• pp.91-94
• /
• 2002

An efficient molecular breeding technique for coffee plants was developed. In order to produce transgenic coffee plants, we established a model transformation procedure via Agrobacterium method. We isolated a gene encoding a protein possessing 7-methylxanthine methyltransferase (theobromine synthase) activity, and it was designated as Coffea arabica 7-methylxanthine methyl transferase; CaMXMT. Using this clone, we produced transgenic coffee plants, in which the expression of CaMXMT is suppressed by double-stranded RNA interference (RNAi) andlor anti-sense methods. The expression pattern of CaMXMT was analyzed by reverse transcription-PCR method and we found that, in the transformed cell lines, the level of transcripts were obviously suppressed by RNAi. The endogenous level of caffeine in the transformed cells was dramatically reduced in comparison with non-transformed cells.

• Journal of Animal Science and Technology
• /
• 제63권4호
• /
• pp.919-933
• /
• 2021

We hypothesized that the unsaturated fatty acid palmitoleic acid (POA) could promote the expression of adipogenic/lipogenic genes in bovine skeletal muscle satellite cells (BSCs). The BSCs were cultured in a growth medium containing 10% fetal bovine serum. When the cells reached 80%-90% confluence, we used the differentiation medium with 5% horse serum for differentiation for 96 h. The differentiation medium contained 50 µM, 100 µM and 200 µM POA. Control BSC were cultured only in differentiation media. Compared with the control BSC, the POA BSC significantly up-regulated the expression of paired box 3 (Pax3) and paired box 7 (Pax7) and down-regulated myogenin gene expression (p < 0.01), which indicates a depression in muscle fiber development. However, all POA treatments up-regulated the expression of the adipocyte transcription factors peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein alpha and beta (C/EBP α and C/EBP β), and other genes (p < 0.01) and increased the expression of PAT-family proteins and the concentration of adiponectin in the media. These results indicate that POA can convert part of BSCs into adipocytes.

• Nuclear Engineering and Technology
• /
• 제54권6호
• /
• pp.2023-2030
• /
• 2022

A novel pulse fitting analysis (PFA) method is presented for the acquisition of nuclear spectra. The charging process of the feedback capacitor in the resistive feedback charge-sensitive preamplifier is equivalent to the impulsive pulse, and its impulse response function (IRF) can be obtained by non-linear fitting of the falling edge of the nuclear pulse. The integral of the IRF excluding the baseline represents the energy deposition of the particles in the detector. In addition, since the non-linear fitting process in PFA method is difficult to achieve in the conventional architecture of spectroscopy system, a new multi-channel analyzer (MCA) based on Zynq SoC is proposed, which transmits all the data of nuclear pulses from the programmable logic (PL) to the processing system (PS) by high-speed AXI-Stream in order to implement PFA method with precision. The linearity of new MCA has been tested. The spectrum of ¹³⁷Cs was obtained using LaBr₃(Ce) scintillator detector, and was compared with commercial MCA by ORTEC. The results of tests indicate that the MCA based on PFA method has the same performance as the commercial MCA based on pulse height analysis (PHA) method and excellent linearity for γ-rays with different energies, which infers that PFA method is an effective and promising method for the acquisition of spectra. Furthermore, it provides a new solution for nuclear pulse processing algorithms involving regression and iterative processes.

• 한국ITS학회:학술대회논문집
• /
• 한국ITS학회 2004년도 제3회 정기총회 및 추계학술대회
• /
• pp.224-227
• /
• 2004

• 한국산학기술학회논문지
• /
• 제19권2호
• /
• pp.270-278
• /
• 2018

본 연구는 어린이집 유아교사의 질적 향상에 대한 발전방향을 모색하기 위해 CIPP 평가 모형에 기반하여 어린이집 유아교사가 이수한 보수교육에 대한 평가를 실시하고자 한다. 이를 위하여 충남 및 대전광역시에 소재한 어린이집에서 근무하는 유아교사 203명을 대상으로 설문조사를 실시하였다. 수집된 자료를 바탕으로 SPSS 21.0을 사용하여 자료를 분석하였고, 그 결과는 다음과 같다. 어린이집 유아교사 대상 보수교육은 목표제시와 사전분석과 같은 교육상황은 다른 평가영역보다 긍정적이었으나, 유아교사 개인요인과 같은 교육과정은 상대적으로 수정과 보완이 필요한 영역으로 평가 되었다. 유아교사 개인의 특성을 반영하지 못한 획일적인 교육은 체계적이고 효과적인 교육이 이루어지는 데 한계가 있을 수 있으므로 교사의 현장과 실태를 고려하여 계획이 이루어져야 하고 교사의 현장요구가 반영되어야 한다. 따라서 본 연구는 어린이집 유아교사 대상 보수교육의 평가를 통해 보수교육프로그램의 실정과 추후 보다 바람직한 어린이집 보수교육이 이루어지도록하기 위한 기초자료가 될 것이며, 보수교육 내실화를 위한 유아교사의 자질 향상 프로그램 개발에 기초자료로 활용될 것으로 사료된다.

• 동아시아식생활학회지
• /
• 제25권2호
• /
• pp.372-385
• /
• 2015

The purpose of this study was to develop and evaluate a nutritional education program for preschool children in association with Center for Children's Foodservice Management, childcare facilities and home in order to provide nutritional knowledge and change food attitudes in children. The program was repeated three times using the same educational theme and consisted three steps. Step 1 involved visiting education with teaching tools by a dietician. Step 2 involved home education with a worksheet and participating in events with parents. Step 3 involved repeated education with a textbook by a preschool teacher education reports sent to the center. The subjects of this study were 3 to 5-year-old children at 89 childcare facilities located in Busanjingu. Interest in meals at childcare facilities, dietary guidance for parents of children, and dietary attitudes of children were evaluated before and after education. Number of parents interested in meals managed by childcare facilities (kitchen visiting, meals observation, progress of children's education) increased after education. Mean scores for meal awareness in parents in the form of three questions (proper food distribution, hand washing before mealtime, nutritional and hygienic satisfaction with meals) significantly (p<0.05) increased after education. Mean dietary guidance for parents' scores for all questions except table manner significantly (p<0.01) increased after education. Mean dietary attitudes for children in the form of six questions (try to eat various vegetable, eat meals without leaving, wash hands before mealtime, eat cleanly, reduce sodium intake and keep table manner) also significantly (p<0.001) increased after education. As a results, the nutritional education program applied in this study, positive influenced children's nutritional knowledge and dietary attitudes, and parents' dietary guidance of children.