• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.5
• /
• pp.743-748
• /
• 2017

Objective: Based on rapid advancement of genetic modification techniques, genomic editing is expected to become the most efficient tool for improvement of economic traits in livestock as well as poultry. In this study, we examined and verified the nickase of mutated CRISPR-associated protein 9 (Cas9) to modulate the specific target gene in chicken DF1 cells. Methods: Chicken myostatin which inhibits muscle cell growth and differentiation during myogenesis was targeted to be deleted and mutated by the Cas9-D10A nickase. After co-transfection of the nickase expression vector with green fluorescent gene (GFP) gene and targeted multiplex guide RNAs (gRNAs), the GFP-positive cells were sorted out by fluorescence-activated cell sorting procedure. Results: Through the genotyping analysis of the knockout cells, the mutant induction efficiency was 100% in the targeted site. Number of the deleted nucleotides ranged from 2 to 39 nucleotide deletion. There was no phenotypic difference between regular cells and knockout cells. However, myostatin protein was not apparently detected in the knockout cells by Western blotting. Additionally, six off-target sites were predicted and analyzed but any non-specific mutation in the off-target sites was not observed. Conclusion: The knockout technical platform with the nickase and multiplex gRNAs can be efficiently and stablely applied to functional genomics study in poultry and finally adapted to generate the knockout poultry for agribio industry.

• BMB Reports
• /
• v.50 no.10
• /
• pp.528-533
• /
• 2017

Peroxiredoxin I (Prx I) plays an important role as a reactive oxygen species (ROS) scavenger in protecting and maintaining cellular homeostasis; however, the underlying mechanisms are not well understood. Here, we identified a critical role of Prx I in protecting cells against ROS-mediated cellular senescence by suppression of $p16^{INK4a}$ expression. Compared to wild-type mouse embryonic fibroblasts (WT-MEFs), Prx $I^{-/-}$ MEFs exhibited senescence-associated phenotypes. Moreover, the aged Prx $I^{-/-}$ mice showed an increased number of cells with senescence associated-${\beta}$-galactosidase (SA-${\beta}$-gal) activity in a variety of tissues. Increased ROS levels and SA-${\beta}$-gal activity, and reduction of chemical antioxidant in Prx $I^{-/-}$ MEF further supported an essential role of Prx I peroxidase activity in cellular senescence that is mediated by oxidative stress. The up-regulation of $p16^{INK4a}$ expression in Prx $I^{-/-}$ and suppression by overexpression of Prx I indicate that Prx I possibly modulate cellular senescence through $ROS/p16^{INK4a}$ pathway.

• Proceedings of the Korea Contents Association Conference
• /
• 2005.11a
• /
• pp.137-143
• /
• 2005

The popularization of IEEE1394(firewire) configuration in Desktop PC have done much to popularize Non-Linear video editing. DVD has been taking the lead in an age as the movie storage medium which represents a recent decade the place because of decreasing in cost of DVD-RW. But, it is the actual condition which has been had any research data about the process which produces the high quality movie of DVD to most ordinary people. Taking into account to capture the image with Avid Xpress DV and Adobe Premiere Pro which are representative video editing softwares in Desktop PC in general, we would like to close by proposing that DVD Authoring Process is available to keep high quality image in Desktop PC by the using of comparison and analysis of the image quality and export data which are able to be happened to the procedure of digitalization of video Authoring using of Adobe Encore.

• The Journal of the Korea Contents Association
• /
• v.7 no.12
• /
• pp.95-104
• /
• 2007

As the broadcasting system rapidly migrates to the HD contents production, a broadcasting professional training program has increasingly requested, which provides a professional ability keeping up with new demands and a systematic well-organized education program. This paper utilized a DACUM(developing a curriculum) method for a video editor's job analysis and presented a job model of video editing. With a DACUM job analysis, we retrieved 9 essential job duties and 71 tasks, and then examined their importance, difficulty, frequency, and entry level tasks that are required before job hire. We also established a well-form structure of the job analysis results, completed a DACUM research chart, and consequently built a video editor's job model in TV broadcasting and video production areas. The proposed model is expected to be used as a fundamental material for a future job organization of video editors in TV broadcasting and video production areas, a development of the educational curriculum, and a priority decision of on-the-job training programs.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2003.10a
• /
• pp.500-503
• /
• 2003

XML been using extensively by standard of data exchanging in the Internet is observed by a technology to replace existent document creation language of HTML etc. Biggest characteristic of this XML is that logic information and physical information that express style of document that do that express structural substance of document were detached. Hereupon, W3C advised XSL that oner style function of form similar to HTML for XML's style and data conversion. Also, XSL's conversion function offers function that change XML document to other data format, and can describe style information through conversion of various document format. But, a XML document conversion technology that use XSLT know-how in domestic is unprepared real condition, and necessity about solution that can edit XSLT document efficiently is putting. This paper does XML document so that conversion and output are available in various document format. And offered research of XSLT document editing system that can edit and create XSLT document efficiently under WYSIWYG environment.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.13 no.6
• /
• pp.1058-1064
• /
• 2009

According to development of Information Technology and generalization of internet, practical use field of geography information became various. Therefor various Geographic Information System (GIS) constructed to manage geography information efficiently. However, geography information data of various form is depending on graphic authorizing tool of various form being not normalized each other. So, OGC(Open Geospatial Consortium) proposed GML(Geography Markup Language) that describe normalized geography information data that can apply mutually and W3C proposed SVG(Scalable Vector Graphics) of vector base. In this paper creates GML data of XML base for geography information data processing to vector graphic object, and design and implementation of GML document editing and translation system that define code converter that create GML document through created graphic objects and change vector graphic to logic structure of XML base.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.29 no.3
• /
• pp.81-89
• /
• 2001

The purpose of this research is to provide for some useful data for computer-related courses of the landscape science, assuming that computers are being and should be used more and more in landscape architecture and therefore, that landscape science students need to respond positively to such a trend. For this purpose, landscape architecture studios and their landscape architects were surveyed for their computer and its program uses as well as their satisfaction with the utility of computer for their landscape works. The results of this research are as follows; 1) 10.7% of the landscape architects surveyed answered that they had learned about their necessary softwares at school, while 61.6% of the studios surveyed were less satisfied with their employees' poor computing ability. Such findings suggest a lack of computer-related courses in landscape science and therefore, that more computer-related courses should be introduced for landscape science students together with the necessary software education. 2) Too few landscape softwares are being used by the landscape architecture studios; such softwares as AutoCAD, Photoshop, 3D Max, Excel, PowerPoint and 한글 account for more than 90% of the landscape architecture softwares being used currently. Quite naturally, landscape students need to be trained on these softwares above all. 3) It was found hat he area of work using the computer most was ˝design works˝(84.5%), followed by ˝documentation˝(83.8%), ˝image editing˝(75.1%), ˝cost calculation˝(68.2%), ˝presentation˝(68.1%), ˝analysis˝(37.8%) and ˝rendering˝(35%). It was regretable to discover that such areas requiring more computing work as ˝analysis˝ and ˝rendering˝ were still worked manually. So it sis deemed necessary for landscape science courses to enhance their computer education of such areas first of all.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.17 no.4
• /
• pp.857-862
• /
• 2013

Due to the development of the online and mobile, cases of introducing CMS(Contents Management System) and WCMS(Web Contents Management System) to manage Web-Contents of individual and company's homepage are continuously increasing. In this paper, it is necessary to develop WCMS that can establish GUI(Graphic User Interface) based WCMS and edit contents using more concise procedures. In this paper, by reconfiguring WCMS-based Web contents using user-definition, we designed the system that can effectively and conveniently edit web contents as GUI-based. Also, we studied the intuitive and convenient web-contents management methods through researches using simplified logic. Therefore, proposed WCMS based Web-contents editing system allows the users to edit and modify the web contents much more quickly and conveniently compared to the existing web-contents editing system.

• Journal of the Korea Computer Industry Society
• /
• v.8 no.2
• /
• pp.103-106
• /
• 2007

In order to construct a Video Database, shot change detection should be made first. But, because these processes are not automated perfectly, we need a lot of time and efforts now. And, there are many shot change detection algorithms, which can't always insure the perfect result because of the editing effects such as cut, wipe, and dissolves used in film production. Therefore, in order to receive the exact shot change, It needs the verification and correction by manual processing at any cost. Spatiotemporal slice is a simple image condensing method for the content changes of video. The editing effects are expressed on the Spatiotemporal slice in the visually noticed form of vertical line, diagonal line, curved line and gradual color changes, etc. Accordingly the parts doubted as a shot change can be easily detected by the change of the Spatiotemporal slice without replaying the video. The system proposed in this study makes it possible to delete the false detected key frames, and create the undetected key frames on the Spatiotemporal slice.

• The Korean Journal of Physiology and Pharmacology
• /
• v.22 no.4
• /
• pp.457-465
• /
• 2018

The expression of BCL-2 interacting cell death suppressor (BIS), an anti-stress or anti-apoptotic protein, has been shown to be regulated at the transcriptional level by heat shock factor 1 (HSF1) upon various stresses. Recently, HSF1 was also shown to bind to BIS, but the significance of these protein-protein interactions on HSF1 activity has not been fully defined. In the present study, we observed that complete depletion of BIS using a CRISPR/Cas9 system in A549 non-small cell lung cancer did not affect the induction of heat shock protein (HSP) 70 and HSP27 mRNAs under various stress conditions such as heat shock, proteotoxic stress, and oxidative stress. The lack of a functional association of BIS with HSF1 activity was also demonstrated by transient downregulation of BIS by siRNA in A549 and U87 glioblastoma cells. Endogenous BIS mRNA levels were significantly suppressed in BIS knockout (KO) A549 cells compared to BIS wild type (WT) A549 cells at the constitutive and inducible levels. The promoter activities of BIS and HSP70 as well as the degradation rate of BIS mRNA were not influenced by depletion of BIS. In addition, the expression levels of the mutant BIS construct, in which 14 bp were deleted as in BIS-KO A549 cells, were not different from those of the WT BIS construct, indicating that mRNA stability was not the mechanism for autoregulation of BIS. Our results suggested that BIS was not required for HSF1 activity, but was required for its own expression, which involved an HSF1-independent pathway.