• Title/Summary/Keyword: Ecotype

Search Result 104, Processing Time 0.023 seconds

Bioconversion of Piceid to Piceid Glucoside Using Amylosucrase from Alteromonas macleodii Deep Ecotype

  • Park, Hyunsu;Kim, Jieun;Park, Ji-Hae;Baek, Nam-In;Park, Cheon-Seok;Lee, Hee-Seob;Cha, Jaeho
    • Journal of Microbiology and Biotechnology
    • /
    • v.22 no.12
    • /
    • pp.1698-1704
    • /
    • 2012
  • Resveratrol, or its glycoside form piceid, is a dietary antioxidant polyphenolic compound, found in grapes and red wine that has been shown to have protective effects against cardiovascular disease. However, very low water solubility of the compound may limit its application in the food and pharmaceutical industries. The amylosucrase (AMAS) of Alteromonas macleodii Deep ecotype was expressed in Escherichia coli and showed high glycosyltransferase activity to produce the glucosyl piceid when piceid was used as an acceptor. The conversion yield of piceid glucoside was 35.2%. Biotransformation using culture of the E. coli harboring the amas gene increased the yield up to 70.8%. The transfer product was purified by reverse phase chromatography and recycling preparative HPLC, and the molecular structure of the piceid glucoside was determined using NMR spectroscopy. The piceid glucoside was identified as glucosyl-${\alpha}$-($1{\rightarrow}4$)-piceid. The solubility of glucosyl piceid was 5.26 and 1.14 times higher than those of resveratrol and piceid, respectively. It is anticipated that dietary intake of this compound is more effective by enhancing the bioavailability of resveratrol in the human body because of its hydrophilic properties in the intestinal fluid.

Evaluation of Characteristics and Forage Production for Bermudagrass (Cynodon dactylon) and Bahiagrass (Paspalum notatum) in Jeju (제주지역에서 버뮤다그라스 및 바히아그라스의 생육특성 및 사초생산성 평가)

  • Park, Hyung-Soo;Park, Nam-Gun;Kim, Jong-Gun;Choi, Ki-Choon;Lim, Young-Chul;Choi, Gi-Jun;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.32 no.2
    • /
    • pp.131-138
    • /
    • 2012
  • This study was conducted to evaluate the characteristics and forage production of bermudagrass (Cynodon dactylon) and bahiagrass (Paspalum notatum) in Jeju. Bermudagrass cultivars evaluated were Common and Ecotype. Bahiagrass cultivars evaluated were Tifton 9 and Argentine. Two warm season grasses were established at the Subtropical Animal Experiment Station in spring 2007. Emergence of bremudagrass and bahiagrass was observed approximately 16 days and 28 days after seeding, respectively. The heading dates of bermudagrass and bahiagrass were on 26 May and in mid-July, respectively. Bermudagrass cultivars had higher dry matter (DM) than bahiagrass at first harvest. Dry matter yield of bahiagrass was higher than that of bermudagrass (p<0.05). Peak forage DM production of bermudagrass and bahiagrass cultivars was in June and July, respectively. The content of crude protein (CP) and total digestibility nutrient (TDN) of bermudagrass cultivars were higher than those of bahiagrass during the first harvest. Acid detergent fiber (ADF) and in vitro DM digestibility (IVDMD) were similar across the four cultivars. In Jeju, bermudagrass and bahiagrass provide a useful option for supplemental summer forage in most livestock forage systems.

Investigation of Physiological and Yield Responses to Temperature Increases in Northern-ecotype Garlic (Allium sativum L. ) 'Uiseong' in Temperature Gradient Tunnels (한지형 마늘 '의성'의 온도구배하우스내 온도상승에 따른 생육 및 생리장해 조사)

  • Byung-Hyuk Kim;Min-Seon Choi;Chun Hwan Kim;Minji Shin;Seong Eun Lee;Kyung Hwan Moon;Hyun-Hee Han
    • Korean Journal of Agricultural and Forest Meteorology
    • /
    • v.25 no.4
    • /
    • pp.276-283
    • /
    • 2023
  • Garlic (Allium sativum L.) is one of the most important vegetables used in various foods in Korea and many countries. The growth of garlic is influenced by various abiotic factors such as cultivation temperature, humidity, minimum temperature duration, and photoperiod. This study investigated the effects of increasing temperatures on the plant growth of the northern- ecotype garlic 'Uiseong' in a temperature gradient tunnel. As a result, temperature increase led to decreases in the bulb diameter, weight, and clove pieces of garlic. The rise of cultivation temperature increased the occurrence rate of incomplete bolting in the Northern-ecotype garlic 'Uiseong', resulting in decreases in productivity and a decrease in the yield of marketable garlic, indicating that temperature increases affect the development of garlic bulb formation. The findings of this study are expected to contribute as foundational data for understanding the growth responses of the northern-ecotype 'Uiseong' to increasing cultivation temperatures. The results of this study can be used to develop designing garlic growth models. In addition, the results of this study can improve understanding the interaction between increased temperature and garlic growth.

Functional Analysis of BCTV ORF L4 by Site-directed Mutagenesis (Site-directed mutagenesis를 이용한 BCTV ORF L4의 기능 분석)

  • 박을용;이석찬
    • Korean Journal Plant Pathology
    • /
    • v.14 no.5
    • /
    • pp.513-518
    • /
    • 1998
  • Beet curly top virus (BCTV) mutant has been constructed in vitro that contain G-to-T transversions at nucleotide 2727 within overlapping open reading frames (ORFs) L1 and L4. The mutations introduce termination codon in ORF L4 without affecting the amino acid encoded by ORF L1. When agroinoculated into Arabidopsis thaliana the mutant caused mild stunting and stem curling, but not the callus induction and hyperlasia on infected tissues of Sei-O ecotype. However, this mutant was not infectious on Col-O. Levels of single stranded DNA forms were similar in mutant and wild type BCTV infections. The DNA quantitation data showed that the DNA of BCTV-L4 mutant virus was accumulated in shoot tips, infection origin and roots with similar levels to those of wild type virus infected. Three tissues of asymptomatic ecotype Col-O also had as much as virus DNA from wild type virus infections. In both ecotypes infected with BCTV-Logan and BCTV-L4 mutant, root tissues contained more virus DNA than any other tissues by the Southern hybridization data. The results suggest that ORF L4 encodes a functional protein that is a major determinant of pathogenesis that might affect the hyperplastic response of the host to BCTV infection.

  • PDF

Classification of Ecotypical and Maturing Groups of Perilla varieties (들깨 품종의 생태형과 성숙군의 분류)

  • Ik-Sang Yu;Eun-Woong Lee
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.14
    • /
    • pp.133-137
    • /
    • 1973
  • In order to classify the ecotypical and maturing groups 136 Korean local and 4 Japanese Perilla varieties were sown at Suwon on May 20, 1972, and investigations were made on days to flowering, days to maturing and growth duration. Results obtained were summarized as follows: 1. Ecotype was classified from 6 groups with combination of the long and short duration from sowing to flowering and maturing period. The classified symbols of groups were Ia, Ib, IIa, IIIa, and IIIb. I: long duration. to flowering II: medium duration to flowering III: short duration to flowering a: long duration to maturing b: short duration to maturing Korean local varieties were distributed 3 ecotypes of IIa, IIb and IIIa, however, Japanese varieties did 2 ecotypes of IIa and IIb, respectively. 2. Maturing group was classified from 3 groups with long (I), medium (II), and short (III) growth durations. Korean local varieties belong to II and III groups but Japanese varieties belong to I and II groups, respectively. 3. Three were highly significant positive correlation (0.685) between ecotypical and maturing group. Ecotype was more related with days to flowering and growth duration, however, maturing group was related with days to flowering, days for maturing, and growth duration. Korean local varieties, more distributed into ecotype than maturing group, were attributed to differences of environmental conditions and cultural practices at different provinces.

  • PDF

Prediction of Soybean Growth in the Northern Region based on Growth Data from the Southern Regions of the Korean Peninsula (한반도 남부지역 생육 데이터 기반 북방지역 콩 생육 예측)

  • Ye Rin Kim;Jong hyuk Kim;Il Rae Rho
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.68 no.4
    • /
    • pp.285-293
    • /
    • 2023
  • This study was conducted to determine the sowing limit period and predict growth in the northern region based on accumulative temperature for each growth stage of soybean cultivated in the southern regions of the Korean Peninsula. First, the results of a demonstration test in the central region (Yeoncheon) of the Korean Peninsula were very similar to the predicted and actual values on the date by growth stage obtained through cultivation. This method was then applied to seven agricultural climatic zones in the northern Korean Peninsula. The results predicted that regardless of ecotype, soybean could be grown and harvested in the southern and northern parts of Mt. Suyang, south of the East Sea, and in the central and northern inland areas. However, it was predicted that no ecotype could be grown and harvested normally in the northern alpine region. Furthermore, north of the East Sea, the prediction indicated that early and mid-maturing cultivars could be grown and harvested normally, but middle-late maturing cultivars appeared to lack the number of growth days. The sowing limit period also varied depending on the ecotype, although it was reached earlier as higher latitudes were approached; the period ranged from May 16 to June 26 in the northern and southern parts of Mt. Suyang, north and south of the East Sea, and central and northern inland areas. Furthermore, all ecotypes of the northern alpine region, as well as mid-late maturing cultivars in the north of the East Sea, were predicted to be unable to grow normally owing to the lack of number of days required for soybean growth and development.