• Asian-Australasian Journal of Animal Sciences
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• 제20권7호
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• pp.1030-1038
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• 2007

Results on localization of growth hormone receptor (GHR), interaction of growth hormone (GH) with receptor in buffalo adipose tissue and identification of activated signaling molecules in the action of GH are presented. Bovine GH (bGH) was labeled with fluorescein or biotin. Fluorescein-labelled bGH was used for localization of GHRs in buffalo adipocytes. The receptors were present on the cell surface. The affinity of binding of GH to its receptor was determined by designing an experiment in which buffalo adipose tissue explants, biotinylated GH and streptavidin-peroxidase conjugate were employed. The affinity constant was calculated to be $2{\times}10^8M^{-1}$. The receptor density on adipose tissue was found to be 1 femto mole per mg of tissue. Signalling molecules generated in the action of GH were tentatively identified by employing Western blot and enhanced chemiluminescence techniques using anti-phosphotyrosine antibody. Based on molecular weights of proteins reactive to anti-phosphotyrosine antibody, three signaling molecules viz. insulin receptor substrate, Janus activated kinase (Jak) and mitogen activated protein were tentatively identified. These signaling molecules appeared in a time (incubation time of explants with growth hormone) dependent way. The activation of Jak2 was confirmed by employing anti-Jak2 antibody in a Western blot. The activation of Jak2 occurred during 5 min incubation of buffalo adipose tissue explants with GH and incubation for an additional period, viz. 30 min. or 60 min., resulted in a drastic reduction in activation. The results suggest that Jak2 activation is an early event in the action of GH in buffalo adipose tissue.

• 한국간호교육학회지
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• 제9권2호
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• pp.234-243
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• 2003

The researcher has studied the types of accidents and injuries that most often occur to infants, toddlers and preschool children. Using this knowledge, a first aid program was developed for mothers. This researcher used a Quasi experimental study which consisted of a nonequivalent control group pretest - posttest design for injury first-aid knowle. The experimental group consisted of 32 mothers, and the control group consisted of 29. The education program consisted of the types of injury, the structure and function of skin, the methods of obsevation, first-aid awareness, and the standard of professional support in case of contusion, abrasion, laceration, fracture and burn. The education program was developed and based on 'the systemetic design of instruction' by Dick & Carey(1996) and utilized multimedia text book, pictures, examples, practice and discussions to increase understanding and effectiveness of learning. The data for this study was collected from September to early November, 2001. There were two fomative evauations, pretest and posttest with an intervention of education program. The analysis of the collected data was analyzed by descriptive analysis, ANOVA, t-test and paired t-test using the SPSS 10.0 program. The results as follows; 1. The experimental group, who was given an education program before the test, got higher marks on the injury first-aid knowledge than the control group. There was a significant difference in knowledge between experimental group and control group(t=6.578, p=.000). 2. The experimental group got higher marks on the action evaluation than the control group. There were significant differences in the certainity of action (t=8.546, p=.000) and the accuracy of action (t=7.654, p=.000) between experimental group and control group. This study examined how a first aid education program increased effectiveness in the knowledge and action of injury first-aid.

• 한국유기농업학회:학술대회논문집
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• 한국유기농학회 2009년도 하반기 학술대회
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• pp.281-281
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• 2009

Although national and regional environmentally friend agriculture (EFA) and food development programmes such as the Life-Food Development Plan (LFDP) have been established in Korea, some policy measures in these programmes seem to be unsuitable for Korean organic farming development. Policy measures tend to support external input purchases of organic fertilizers rather than market development actions such as providing consumer information, research, education, training and statistical data collection. The development of an organic action plan (OAP) for Korea is therefore considered essential for the sustainable future of organic farming in Korea. The purposes of OAP are 1) to define and set the clear goals/targets for the organic sector development, 2) to integrate various organic stakeholders and public institutions in partnership, 3)to focus on specific issues with tailored measures and 4) to integrate and develop different policy measures (Stolze, 2005). Most EU member state countries have developed their own OAPs and each reflects its own priorities with regard to organic sector development. This study compares and contrasts the Welsh, England and EU OAP with the Korean Jeonnam Life-Food Development Plan (LFDP) in order to facilitate the development of the organic food and farming sector in Korea. Early action plan, for example, the first Welsh OAP(1999) focused support on developing the supply of organic products whereas later action plans (e.g. England OAPs in 2002 and 2004 and the second Welsh OAP in 2005) focussed more on developing consumer demands for organic products. The EU OAP (2004) also aims at market support related to consumer demand and then organic farming production for its environmental and other social benefits. OAPs not only provide specific issue-solving tools but also perform a role as providing a focus for organic sector development as a whole. The Korean LFDP provides issue-solving tools but plays no regulatory role such as policy development, harmonizing various policy measures and conflicting factors and providing evaluation tools for further development. A national-level OAP could also facilitate international trade of organic products. To achieve better harmonized and sustainable approaches for the Korean organic industry, National- as well as regional- regulatory policy systems are urgently required in the form of an Organic Action Plan.

• ALGAE
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• 제19권2호
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• pp.129-137
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• 2004

Since cultural eutrophication has the detrimental effects on estuarine and coastal ecosystems, recognition of early stage of nutrient over-enrichment is critical for effective managements of the ecosystems. Since released nutrients into coastal ecosystems are diluted and dissipated through tidal action and rapid uptakes by marine plants, monitoring of in situ nutrient concentrations may not be useful for detecting early eutrophication on coastal and estuarine ecosystems. To develop an effective indicator of cultural eutrophication using marine plants, tissue N content and area normalized leaf mass of eelgrass, Zostera marina were examined in Kosung Bay and Koje Bay on the south coast of Korea from June 2001 to April 2003. Eelgrass tissue N content exhibited obvious seasonal variations. Leaf N content was highest during winter and early spring and lowest during summer. Eelgrass tissue N content was higher at Kosung Bay site, which has higher sediment organic content, than at Koje Bay site. Area normalized leaf mass showed reverse trend of leaf N content, and consequently, eelgrass leaf N content and leaf mass exhibited strong negative correlation at both study sites. The results of the present study suggested that the ratio of eelgrass leaf N content to area normalized leaf mass can be applied to assess environmental nitrogen conditions on the coastal and estuarine ecosystems.

• International Journal of Oral Biology
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• 제30권4호
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• pp.135-141
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• 2005

Although it has been known that TGF-${\beta}1$ acts as a crucial cofactor in osteoclast differentiation, its mode of action is still unclear. In the present study, we studied the effect of TGF-${\beta}1$ on the differentiation of osteoclast depending on the developmental stages. Murine bone marrow cells were induced to differentiate into mature osteoclasts in the presence of receptor activator of NF-${\kappa}B$ ligand (RANKL) and macrophage colony stimulating factor (M-CSF). In the early stage of the differentiation TRAP(-) mononuclear precursor cells were obtained from nonadherent M-CSF dependent bone marrow cells, which further differentiated into mature osteoclasts. TGF-${\beta}1$ stimulated osteoclast differentiation, which was stronger when cells were stimulated by TGF-${\beta}1$ in the early stage than the later differentiation. TGF-${\beta}1$ increased the expression of RANK and synergistically stimulated RANKL-induced activation of NF-${\kappa}B$ MAP kinase in TRAP(-) mononuclear precursor cells. These results suggest that activation of osteoclast differentiation by TGF-${\beta}1$ may be ascribed to the both increased expression and activation of RANK in the osteoclast differentiation, especially in the early stage of differentiation.

• 대한치과마취과학회지
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• 제1권1호
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• pp.26-31
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• 2001

The wide deep penetrating wound of maxillofacial region should be early closed under emergency general anesthesia for the prevention of complications of bleeding, infection, shock & residual scars. But, if the emergency general anesthesia wound be impossible because of pneumoconiosis, obstructive pulmonary disease & hypovolemic shock, early primary closure should be done under local anesthesia by use of much amount of the anesthetic solution. The maximum dose of dental lidocaine (2% lidocaine with 1 : 100,000 epinephrine) is reported to 7 mg/kg under 500 mg (13.8 ampules) in normal adult. But the maximum permissible dose of dental lidocaine can be changed owing to the general health, rapidity of injection, resorption, distribution & excretion of the drug. The blood level of overdose toxicity is above $4.0{\mu}g/ml$ in central nervous & cardiovascular system. The injection of dental lidocaine 1-4 ampules is attained to the blood level of $1{\mu}g/ml$ in normal healthy adult. The duration of anesthetic action in the dental 2% lidocaine hydrochloride with 1 : 100.000 epinephrine is 45 to 75 minutes and the period to elimination is about 2 to 4 hours. Therefore, authors selected the following anesthetic methods that the first injection of 6 ampules is applied into the deeper periosteal layer for anesthetic action during 1 hour, the second injection into the deeper muscle & fascial layer, the third injection into the superficial muscle and fascial layer, the fourth injection into the proximal skin & subcutaneous tissue and the fifth final injection into the distal skin & subcutaneous tissue. The total 26-28 ampules of dental lidocaine were injected into the wound as the regular time interval during 5-6 hours, but there were no systemic complications, such as, agitation, talkativeness, convulsion and specific change of vital signs and consciousness.

• 한국동물생명공학회지
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• 제35권2호
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• pp.163-170
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• 2020

Partheno Embryo's research is known to play a very important role in identifying the development of embryonic cells or analyzing the genetic mechanisms of embryonic development, but the information on apoptosis formed during the early stage of development on Partheno Embryo is very little. Therefore, this study analyzed whether the embryonic cell death of unit embryos can be inhibited by adding Scriptaid, one of HDACi, which plays a role in demethylation of histone proteins as a method of regulating the cell cycle in the early embryo development of Partheno Embryo. As a result, the differentiation rate was higher in the group that added Scriptaid and FBS, but the cellular development was higher in the group that added pregnant serum to Scriptaid. As a result of analyzing the expression of the gene through IF and PCR, the group with the addition of gestational serum increased the expression of BCL2 and PCNA, which affects the anti-Casp3 action in cell survival. In addition, it is interpreted that treatment of Scriptaid for 16 hours, rather than 24 h treatment lowers the expression of Casp-3, a representative factor of apoptosis, and also increases embryonic development, thus affecting early embryo development. Therefore, it is concluded that the 16-hour treatment of Scriptaid and the use of gestational serum will inhibit cell death in the early embryonic development and increase the development rate of the embryo.

• 약학회지
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• 제38권3호
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• pp.238-249
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• 1994

The purpose of this research was to investigate effects of baicalin on the differentiation of preadipocytes, 3T3-L1, and to characterize the action of baicalin that affect the responses of 3T3-L1 cells during differentiation. In various culture conditions, effects of baicalin and adrenoreceptor agonists such as phenylephrine(PE) and isoproterenol(IPR) on cell differentiation were examined. Also, effects of the drugs on differentiation, triglyceride(TG) contents, expression of insulin receptor, cAMP contents, the cytosolic $Ca^{2+}$ levels, and amount of calmodulin(CaM) were examined. The results suggest that baicalin has adrenergic receptor blocking activity during the process of differentiation of 3T3-L1 cells and that in the early stage of the adipose conversion, the effect of baicalin on the adipocyte differentiation is mediated by the regulation of insulin receptor expression, but by alterations of the cAMP and the calcium metabolism in the late stage. These results also suggest that the action of baicalin may be significant in the lipid metabolism, lipogenic and lipolytic pathways, of adipose cells.

• 한국콘텐츠학회논문지
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• 제5권5호
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• pp.219-227
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• 2005

세계적인 소비자 보호의 추세확산에 따라 선진국은 제조물 책임법을 이미 시행하고 있고, 우리나라는 2002년 7월 시행하게 되었다. 대부분 제조물에 초점을 이루고 있지만 특히, 식품의 경우 많은 소송이 제기될 수 있을 뿐만 아니라 엄청난 피해와 기업의 손실도 막대할 것이다. 따라서 경제력 제고 및 소비자의 안정성 확보를 위한 가이드라인을 제시하고자 한다. 1. 제품정보와 품질안전을 쉽게 이해하기 위한 각종 수단을 강구한다. 2. 모든 PL대응 활동을 문서화하라. 3. 소비자의 피해에 초동 대응하라. 4. 평소에 안전기업 이미지를 구축한다. 5. 품질경영 시스템 수립 및 표준화의 유효한 수단이 ISO9000과 HACCP, PLMS를 시행한다. 6. 협력업체의 PL대응도 지원하라. 7. 업계 공동의 PL대응책을 모색하라.

• Journal of Preventive Medicine and Public Health
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• 제40권6호
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• pp.447-453
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• 2007

Although the New National Health Promotion Plan 2010 target to reduce health inequalities, whether the program will be effective for reducing the health inequalities in Korea remains quite unclear. More and more developed countries have been started to concentrate on comprehensive policies for reducing health inequalities. The health policies of the UK, Netherlands, and Sweden are the most wellknown. I propose that a comprehensive blueprint for tackling health inequalities in Korea should be made and that it must contain five domains: a target, structure and process, life-course approach, area-based approach, and reorganization of health care resources. The target should be based on determinants of health and more attention should be paid to socioeconmic factors. The structure and process require changes from the national health care policy based on medical services to the national health policy that involves the establishment of a Social Deputy?Prime Minister and the strengthening multidisciplinary action. A life-course approach especially focused on the early childhood years. Area-based approach such as the establishment of healthy communities, healthy schools, or healthy work-places which are focused on deprived areas or places is also required. Finally, health care resources should be a greater investment on public resources and strengthening primary care to reduce health inequalities. The policy or intervention studies for tackling health inequalities should be implemented much more in Korea. In addition, it is essential to have political will to encoruage policy action.