International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Effect of TGF-${\beta}1$ on Osteoclast Differentiation

  • Park, Su-Jin (Department of Oral Histology & Developmental Biology, School of Dentistry, Seoul National University) ;
  • Ko, Jea-Seung (Department of Oral Histology & Developmental Biology, School of Dentistry, Seoul National University) ;
  • Kim, Hyun-Man (Department of Oral Histology & Developmental Biology, School of Dentistry, Seoul National University)
  • Published : 2005.12.31
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Abstract

Although it has been known that TGF-${\beta}1$ acts as a crucial cofactor in osteoclast differentiation, its mode of action is still unclear. In the present study, we studied the effect of TGF-${\beta}1$ on the differentiation of osteoclast depending on the developmental stages. Murine bone marrow cells were induced to differentiate into mature osteoclasts in the presence of receptor activator of NF-${\kappa}B$ ligand (RANKL) and macrophage colony stimulating factor (M-CSF). In the early stage of the differentiation TRAP(-) mononuclear precursor cells were obtained from nonadherent M-CSF dependent bone marrow cells, which further differentiated into mature osteoclasts. TGF-${\beta}1$ stimulated osteoclast differentiation, which was stronger when cells were stimulated by TGF-${\beta}1$ in the early stage than the later differentiation. TGF-${\beta}1$ increased the expression of RANK and synergistically stimulated RANKL-induced activation of NF-${\kappa}B$ MAP kinase in TRAP(-) mononuclear precursor cells. These results suggest that activation of osteoclast differentiation by TGF-${\beta}1$ may be ascribed to the both increased expression and activation of RANK in the osteoclast differentiation, especially in the early stage of differentiation.

Keywords

References

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