• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.10 no.4
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• pp.39-47
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• 2015

"Series A Crunch"problem has become worse off as funding gap between angel seed investment and traditional VC Series A investment is getting the larger due to big boom over early startup investment both in Korea and US. The strong needs for new concept of fund alternative such as bridge fund is outstanding to fill up the funding gap in the early stage of venture investment. This research is brought to define the concept of 'Series A Crunch' problem and to diagnose its causes, eventually, popping up 'the concept of Micro VC Fund' to come up with this problem in Korea. Also, this paper suggests the policy alternatives to introduce Mivro VC Fund and accommodate its successful performance.

• The Korean Journal of Zoology
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• v.30 no.3
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• pp.248-260
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• 1987

In order to investigate the importance of the prospective mesodermal and endodermal blastomeres at 32-cell stage in the anis formation, blastomeres were deleted or transplanted into the ventrovegital site of another normal embryo. The results are as follows: When the dorsomesodermal or dorsoendodermal blastomeres were deleted, there was a substantial developmental lesion in the axis structure. However, when the ventromesodermal or ventroendodermal blastomeres were deleted, the formation of an axis structure was nearly normal. The dorsomesodermal or dorsoendodermal blastomeres which were transplanted into the ventral side of the normal 32-cell embryo caused the formation of a secondary body axis, and the capacity of the second axis induction in the dorsomesodermal blastomeres was a little higher than that in the dorsoendodermal blastomeres. These results imply that both the dorsomesodermal and dorsoendodermal blastomeres are involved in the formation of a set of dorsal body structures during early embryogenesis. As well, in order to investigate the axis inducing capacity in the early cleavage embryos, the dorsovegital blastomeres were transplanted into the ventrovegital site at 4-cell, 8-cell and 16-ceIL stage respectively. As a ruts·fIt, a second body axis was formed. Therefore, it seems that the early cleavage embryo as 4-cell stage dorsal blastomeres contain some informations necessary for the axis formation.

• Korean Journal of Child Studies
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• v.30 no.5
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• pp.41-55
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• 2009

This study explored the meaning of beginning teachers' first-year experience in an eco child educare center. Participants were 4 teachers at survival stages of teacher development; they had had the same preservice experience. Data were collected by semi-structured in-depth interviews, participant observation, researcher's field notes, and document collection. Five themes emerged as first year teachers' struggles and strategies for survival : (1) caring for children's daily lives vs. responsibility for formal education, (2) planned lessons vs. ease in teaching, (3) agreement or disagreement on philosophy of running the education program, (4) paradox of teacher empowerment, (5) overcoming struggle through voluntary peer supervision. These results indicated that beginning teacher' experiences at the survival stage varied by contextual and personal factors.

• YAKHAK HOEJI
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• v.43 no.6
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• pp.843-850
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• 1999

We investigated whether endothelium-derived NO and endothelin-1 might result enhanced vasoconstriction induced by administration of norepinephrien (NE) at the early stage of one-kidney, one-clip (1K1C) renal hypertensive rats. We also studied the relation ship of renin-angiotensin system (RAS) using rat aorta in this hypothesis. L-NMMA (30$\mu$M) and L-NAME(30${\mu}M$) enhanced vasoconstriction induced by NE in thoracic aorta of control rats. However angiotensin converting enzyme (ACE) inhibitor didn't. The aorta of 1KIC rats showed a singnificantly exaggerated contractile response to NE as compared with control rats. Rub-bing the endothelium abolished this difference. Ach and SNP-induced vasorelaxation show no significant difference between 1KIC and control rats. The treatment of phosphoramidon (10${\mu}M$) and oral administration of captopril (0.05, w/v%) abolished the exaggerated contractile response to NE at early stage of 1KIC rats. These results suggest that the increase of contractile response at the early phase in 1KIC rat is partially involved in the activation of ACE.

• International Journal of Naval Architecture and Ocean Engineering
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• v.13 no.1
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• pp.352-366
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• 2021

To decrease Europe's harmful emissions, the European Union aims to substantially increase its offshore wind energy capacity. To further develop offshore wind energy, investment in ever-larger construction vessels is necessary. However, this market is characterised by seemingly unpredictable growth of market demand, turbine capacity and distance from shore. Currently it is difficult to deal with such market uncertainty within the ship design process. This research aims to develop a method that is able to deal with market uncertainty in early ship design by increasing knowledge when design freedom is still high. The method uses uncertainty modelling prior to the requirement definition stage by performing global research into the market, and during the concept design stage by iteratively co-evolving the vessel design and business case in parallel. The method consists of three parts; simulating an expected market from data, modelling multiple vessel designs, and an uncertainty model that evaluates the performance of the vessels in the market. The case study into offshore wind foundation installation vessels showed that the method can provide valuable insight into the effect of ship parameters like main dimensions, crane size and ship speed on the performance in an uncertain market. These results were used to create a value robust design, which is capable of handling uncertainty without changes to the vessel. The developed method thus provides a way to deal with market uncertainty in the early ship design process.

• BMB Reports
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• v.55 no.11
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• pp.553-558
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• 2022

Hepatocellular carcinoma (HCC) is dangerous cancer that often evades early detection because it is asymptomatic and an effective detection method is lacking. For people with chronic liver inflammation who are at high risk of developing HCC, a sensitive detection method for HCC is needed. In a meta-analysis of The Cancer Genome Atlas pan-cancer methylation database, we identified a CpG island in the USP44 promoter that is methylated specifically in HCC. We developed methylation-sensitive high-resolution melting (MS-HRM) analysis to measure the methylation levels of the USP promoter in cell-free DNA isolated from patients. Our MS-HRM assay correctly identified 40% of patients with early-stage HCC, whereas the α-fetoprotein test, which is currently used to detect HCC, correctly identified only 25% of early-stage HCC patients. These results demonstrate that USP44 MS-HRM analysis is suitable for HCC surveillance.

• Journal of IKEEE
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• v.16 no.1
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• pp.45-50
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• 2012

This paper proposes the methodology to estimate the routing congestion of modern IC quickly and accurately at the early stage of the design flow. The occurrence of over-congestion in the routing process causes routing failure which then takes unnecessary time to re-design the physical design from the beginning. The precise estimation of routing congestion at the early design stage leads to a successful physical design that minimizes over-congestion which in turn reduces the total design time cost. The proposed estimation method at the block-level floorplan stage measures accurate routing congestion by using the analyzed virtual interconnections of inter/intra blocks, synthesized virtual power/ground and clock networks.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.6
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• pp.9-16
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• 2020

This study aims to assess the effects of specimen thickness (ST) on fatigue crack growth in the early stages of crack propagation and near failure in magnesium alloys. The analysis of variance (ANOVA) method was adopted because fatigue crack propagation in magnesium alloys exhibits statistical behavior. The equality of variance test and residual diagnostics were performed on the grown cracks to confirm the validity of ANOVA by verifying the normal distribution and mutual independence of the residuals and their homoscedasticity. ANOVA confirmed that ST heavily impacts crack growth; i.e., when ST is smaller, cracks grow faster in the early crack propagation stage and break more quickly before the formation of larger cracks. We found that ST significantly affects fatigue crack growth in the early crack propagation stage and near the failure stage in magnesium alloys. The regression model was also used to predict crack formation near the failure stage.

• Animal cells and systems
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• v.13 no.3
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• pp.289-295
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• 2009

Cyclic AMP-mediated signaling pathways regulate a number of cellular functions. In this study, we examined the regulatory role of cAMP signaling pathways in chondrogenesis of chick limb bud mesenchymal cells in vitro. Forskolin, which increases cellular cAMP levels by the activation of adenylate cyclase, enhanced chondrogenic differentiation. Inhibition of PKA with specific inhibitors (H89 or KT5720) blocked pre-cartilage condensation stage, indicating that chondrogenesis is regulated by the increase in cellular cAMP level and subsequent activation of PKA. Downstream signaling pathway of PKA leading to gene expression was investigated by examination of several nuclear transcription factors. Forskolin treatment increased transcription level for a cartilage-specific marker gene Sox9. However, inhibition of PKA with H89 led to restore expression of Sox9, indicating PKA activity was required to regulate the expression of Sox9 in chondrogenesis. In addition, CREB was highly phosphorylated at early stage of mesenchyme culture, and followed by progressive dephosphorylation. CBP and ATF, another CRE related proteins were transiently expressed at the early stage of chondrogenesis with a pattern similar to CREB phosphorylation. Electrophoretic mobility shift assays confirmed that the binding activity of CREB to the CRE is closely correlated to the phosphorylation pattern of CREB. Therefore, cAMP-mediated signal transduction to nuclear events for the induction of genes appeared to be required at the early stage of chick limb bud chondrogenesis.

• Journal of architectural history
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• v.10 no.2 s.26
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• pp.55-71
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• 2001

This study reviewed the preservation status of the Early Western Style Architecture in Korea based on the on-site research of church buildings which are registered as National Cultural Properties. I have investigated 21 church buildings regarding the frequency, content and concept of restoration. The results are summarized as follows First, the churches have been restored extensively in every 17 years on the average. Second, the main restorations are replacement of bricks, painting, waterproofing, repair of roof and timber. And the main changes are extension of plan, removal of Interior posts, and replacement of floor. Third, the main concept of restorations are historical restoration(62%), critical restoration(23%), stylistic restoration(14%). Forth, the category of restoration have been changed, that is extending of incompletion (1st stage), functional improvement and extension of the span of life(2nd stage), improvement of facilities(3rd stage), restoration to the original state and conservation(4th stage). Fifth, the issues of repairing techniques are revival and replacement of weathered bricks, the removal of painting, and structural reinforcement. This study will be able to be used as the fundamental materials to improve the techniques of restoration of Early Western style masonry buildings.