• Journal of Mushroom
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• v.13 no.4
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• pp.319-325
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• 2015

This study was carried out for examining the amount of ganoderic acid A, F, H from fruiting body extracts of the various Ganoderma species. The preserved Ganoderma species are extracted by using three kinds of solvent. Among them, five strains which have the large amount of ganoderic acid A, F, H were selected. First of all, Strains with the highest amount of ganoderic acid A were sorted out acorrding to solvent as follows. ASI 7013 has the highest amounts with 0.988 mg/g from D.W. extracts. And ASI 7023, 7059, 7026, 7060 were in order of content. In EtOH extracts, ASI 7037 has the highest amounts of ganoderic acid A with 0.940 mg/g. And ASI 7021, 7034, 7026, 7038 were in order of content. In MeOH extracts, ASI 7162 has the highest amounts with 0.833 mg/g. And ASI 7091, 7032, 7056, 7125 were in order of content. secondly, Strains with the highest amount of ganoderic acid F were sorted out acorrding to solvent as follows. ASI 7011 has the highest amounts with 0.722 mg/g from D.W. extracts. And ASI 7113, 7011, 7104 were in order of content. In EtoH extracts, ASI 7037 has the highest amounts with 0.811 mg/g. And ASI 7007, 7027, 7012, 7014 were in order of content. In MeoH extracts, ASI 7162 has the highest amounts with 0.833 mg/g. And ASI 7021, 7034, 7026, 7125 were in order of content. Finally, Strains with the highest amount of ganoderic acid H were sorted out acorrding to solvent as follows. ASI 7013 has the highest amounts with 0.985 mg/g in D.W. extracts. And ASI 7059, 7135, 7001, 7141 were in order of content. In EtoH extracts, ASI 7016 has the highest amounts with 2.842 mg/g. And ASI 7089, 7159, 7007, 7002 were in order of content. In MeoH extracts, ASI 7003 has the highest amounts with 6.969 mg/g. And ASI 7034, 7169, 7159, 7103 were in order of content.

• Korean Journal of Ecology and Environment
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• v.34 no.3 s.95
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• pp.153-165
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• 2001

We evaluated the health condition of Pyong-Chang river, the tributary of Han- River, using the Index of Biological Integrity (IBI) and Qualitative Habitat Evaluation Index (QHEI) during September 1999${\sim}$August 2000. The annual mean of IBI,　which was estimated using eleven metrics, was 49 (range: $45{\sim}51$) and the mean of QHEI, which was estimated using seven parameters, was 88 (range: $76{\sim}94$) during　the study. The river health, based on the IBI criteria of Karr (1981), ranged from "excellent" to "good" conditions, while based on the habitat criteria of Plafkin et al. (1989), it ranged from "pristine" (comparable to reference) to "supporting" conditions. Values of IBI showed slight differences between upstream and downstream sites and QHEI values varied weakly depending on characteristics of variables. Regression analyses showed that annual values of QHEI had no functional relations with stream order (p = 1.82; n = 8) but showed some decreases near slight point-sources. This result indicates that conditions of physical habitat did not change highly with increases of the stream order. According to analyses of feed guilds, relative abundance of insectivores, omnivores and carnivores was 85.1%, 3.5% 0.3%, respectively. Also, relative abundance of sensitive and tolerant species was 75% and 4.6%, respectively, while exotic and morphological anomalies were not found in the river. These outcomes indicate that health condition of fish, based on the trophic conditions of U.S. EPA (1993), was excellent in the river. Regression analyses of IBI values against the QHEI showed that the variation of habitat conditions accounted 57% for the variation of the Index of Biological Integrity (p<0.05; $R^2\;=\;0.57$; n = 7).Overall data of IBI and QHEI suggest that the river health in the present is in optimal conditions but may be degradated by acceleration of chemical inputs and physical-habitat disturbance.

• Korean Journal of Ecology and Environment
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• v.38 no.3 s.113
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• pp.361-371
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• 2005

The objective of study was to diagnose integrative ecological health of Daejeon Stream, one of the tributaries of Guem River, during May 2004 ${\sim}$ April 2005. The research approach was primarily based on a Qualitative Habitat Evaluation Index (QHEI) and the Index of Biological Integrity (IBI) using fish assemblage. These outcomes were compared with conventional chemical dataset. For the experiment, four sampling sites were chosen from Daejeon Stream and long-term water quality data during 1995 ${\sim}$ 2004 (obtained from the Ministry of Environment) were analyzed in the spatial and temporal aspects. For the biological health assessment, we developed a stream health assessment model (SHA model) far regional applications. We found that current water quality conditions, based on the COD, BOD, TN and TP, were enhanced by 1.6 ${\sim}$ 5.3 fold over the period of 1995 ${\sim}$ 2004 and that the parameters showed a typical longitudinal decline from the upstream to downstream reach. The differences of water quality between the two reaches were more than 4.4 times, indicating a large spatial variations within the stream. The health conditions, based on the SHA model, averaged 23 and varied from 20 to 26 depending on the sampling stations. Values of the QHEI varied from 39 (Poor condition) to 124 (Cood condition)and values of QHEI in the reach of S2 ${\sim}$ S4 had significantly lower than in the headwater site (S1). Also, biological stream health, based on the criteria of US EPA (1993), was judged as 'Poor condition', in the S4 where TN, TP, BOD and COD were highest. In the meantime, maximum value of SHA (26) was found in the upstream reach (S1) where the water quality and QHEI were best. We also found that compositions of sensitive species showed a linear function with water quality conditions and this pattern was evident in the tolerant species. Thus, the biological stream health, based on the SHA model, matched well water chemistry. Overall outcomes suggest that the biological health impact was a function of chemical degradation and physical habitat quality in the stream.

• Clinical and Experimental Reproductive Medicine
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• v.27 no.2
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• pp.117-131
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• 2000

Objectives: To develop a new immunohistochemical marker system for supplementation of the Noyes histological classification of the endometrium in women of child bearing age with regular menstrual cycles, and to employ this system to evaluate pathologic factors involved in endometriosis, and thus to ascertain if it is useful in diagnosis. Materials and Methods: Endometrial biopsies were sampled from the posterior fundus of 41 (24 proliferative phases, 17 secretory phases) women with regular menstrual cycles (28-32 days), and each sample was immunohistochemically stained according to Noyes et al (1975) for determination of expression for estrogen receptor (ER), progesterone receptor (PR), integrin ${\alpha}_1$, ${\alpha}_4$, ${\beta}_3$, COX-1 and COX-2. Then, the PR, integrin ${\beta}_3$ and COX-2 which were clearly expressed in the luteal phase was with endometrial samples were obtained from 20 cases of normal patients (group 1) and 25 cases with endometriosis (group 2) after confirming the day of ovulation by sex steroid level measurements 7-8 days after ovulation Results: In the regular menstruation group the expression of ER showed a tendency to be increased in the proliferative phase and decreased in the secretory phase, and was the highest in the proliferative phase. However, PR in the stromal cells showed no change in the entire menstrual cycle while in the epithelial cells, PR reached a peak in the late proliferative phase and was almost absent in the secretory phase. Integrin (${\alpha}_1$, ${\alpha}_4$, and ${\beta}_3$ expression in the epithelial cells was absent in the proliferative phase but ${\alpha}_1$ was strongly expressed starting from the early secretory phase into the entire secretory phase. ${\alpha}_4$ was expressed strongly in the early and mid secretory phases and disappeared in the late proliferative phase, while ${\beta}_3$ appeared after the mid secretory phase and continued to be expressed until the late secretory phase. Expression in the stromal cells was weak overall and did not show any cyclic pattern. COX-1 expression was shown as a cyclic pattern in the stromal and epithelial cells and was particularly strongly expressed in the mid secretory phase of epithelial cells, and in the mid secretory and menstruation phase of stromal cells. In the endometrial epithelial cells there was strong expression during the entire cycle with stronger expression in the secretory phase compared to the prolferative phase. COX-2 was clearly expressed in the late proliferative, early and mid secretory phases in the stromal cells. No expression was observed in the proliferative phase of the epithelial cells, but which began to appear in the early secretory phase reaching a significant pattern from the mid secretory phase onwards. There was almost no expression in the stromal cells. In the cases with endometriosis showing normal endometrial maturation according to the Noyes classification, PR expression was increased while Integrin-${\beta}_3$의 expression was significantly decreased compared to the normal group. Also, COX-2 expression was slightly decreased in the stromal cells of patients with endometriosis while it was significantly increased in the stromal cells. Conclusion: Immunohistochemical markers can supplement the original Noyes classification of histological endometrial dating and therefore ascertain existing pathologic conditions. Particularly for patients with endometriosis with normally mature endometrial cells, changes in COX-2 and integrin expression patterns may assist in elucidating pathophysiologic mechanisms and therefore aid in the diagnosis of abnormal implantation conditions, and consequently determine a treatment modality.

• Clinical and Experimental Reproductive Medicine
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• v.36 no.1
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• pp.55-61
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• 2009

Objective: To compare the clinical outcomes of ICSI with sperm retrieved from testicular tissue in patients with obstructive azoospermia (OA) or hypospermatogenesis (HS). Methods: From January 2003 through December 2006, 155 patients with OA (241 cycles) and 28 patients with HS (34 cycles) were included in this study. We compared clinical outcomes of ICSI with testicular sperm such as fertilization rate, implantation rate, clinical pregnancy rate and delivery rate. Data were statistically analyzed using t-test and ${\chi}^2$-test. Results: Testicular spermatozoa could not be retrieved in 1 out of the 21 cycles where fresh testicular sperm extraction in HS patients. Fertilization rate (FR) was significantly higher in OA than HS (75.6 % vs. 62.6%, p<0.001). Cleavage rate (CR) per fertilized zygote was also significantly higher in OA than that in HS (66.8% vs. 54.8% p<0.001). However, there were no significant differences in good embryo rate (GER), clinical pregnancy rate (CPR), implantation rate (IR) and delivery rate (DR). Conclusion: Our results show that testicular sperm of HS does not affect CPR, IR, and DR although it has shown reduced FR and CR.

• Journal of agricultural medicine and community health
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• v.25 no.2
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• pp.275-292
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• 2000

The purpose of this study was to analyze the relationship between knowledge, self-efficacy and quality of life of patients suffering from rheumatic arthritis. The data were collected from 100 rheumatic arthritis patients 1 university hospital, K rheumatic clinic in kwang-ju city for 20 days from March 2 to March 22, 1999, by means of questionnaires. The instruments used in this study were the knowledge scale developed by Kim, the self-efficacy scale developed by Long et al and the quality of life scale developed by Rho. The data were analyzed by t-test, ANOVA, Pearson's Correlation Coefficient, and Stepwise Multiple Regression by using the SAS program. The results of this study were summarized as follows : 1. The total mean score of knowledge was 15.01(SD${\pm}$3.08), In self-efficacy, the total mean score was 1030.40(SD${\pm}$233.42), In quality of life, the total mean score was 135.19(SD${\pm}$11.07). 2. The relationship between general characteristics and knowledge were significant difference in sex(t=19.03, p=.00), occupation(F=2.34, p=.03), types of exercise (F=2.95, p=.0.3), and time of exercise(F=3.20, p.=02). 3. The relationship between general characteristics and self-efficacy were significant difference in religion(F=2.75, p=.04), in monthly salary(F=3.64, p=.01), in occupation(F=2.30, p=.03), in period of rheumatic arthritis(F=2.70, F=.03), in time of exercise(F=3.77, P=.01), and in effectiveness of exercise (F=4.56, p= .02). 4. The relationship between general characteristics and quality of life were significant difference in age(F=3.36, p=.01), monthly income(F=3.11, p=.02), types of housing(t=4.93, p=.02), arid time of exercise(F=3.03, p=.03). 5. There was not significant correlation between the subjects knowledge and quality of life. 6. There was significant correlation between the self-efficacy and quality of life(r=462, p<.011). 7. The main factor influencing on quality of life was self-efficacy(21.4%).

• Clinical and Experimental Reproductive Medicine
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• v.29 no.1
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• pp.1-12
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• 2002

Objective: In order to acquire the technique for the establishment of human embryonic stem cells (ESe) derived from the human frozen-thawed embryos produced in IVF-ET program, this study was performed to establish mouse ESC derived from the in vitro fertilized embryos. Materials and Methods: After Fl hybrid (C57BL female $\times$ CBA mael) female mice were superovulated with PMSG and hCG treatment, their oocytes were retrieved and inseminated, and the fertilized embryos were cultured for 96-120 hours until the expected stages of blastocysts were obtained. To isolate the inner cell mass (ICM), either the blastocysts were treated with immunosurgery, or the whole embryos were cultured for 4 days. Isolated ICMs were then cultured onto STO feeder cell layer, and the resultant ICM colonies were subcultured with trypsin-EDTA treatment. During the subculture process, ESC-like cell colonies were observed with phase contrast microscopy. To identify ESC in the subcultured ESC-like cell colonies, alkaline phosphatase activity and Oct-4 (octamer-binding transcription factor-4) expression were examined by immunohistochemistry and RT-PCR, respectively. To examine the spontaneous differentiation, ESC-like cell colonies were cultured without STO feeder cell layer and leukemia inhibitory factor (LIF). Results: Seven ESC-like cell lines were established from ICMs isolated from the in vitro fertilized embryos. According to the developmental stage, the growth of ICMs isolated from the expanded blastocysts was significantly better than that of ICMs isolated from the hatched blastocysts (80.3% vs. 58.7%, p<0.05). ESC-like cell colonies were only obtained from ICMs of expanded blastocysts. However, the ICMs isolated from the embryos treated with immunosurgery were poorly grown and frequently differentiated during the culture process. The established ESC-like cell colonies were positively stained with alkaline phosphatase and expressed Oct-4, and their morphology resembled that observed in the previously reported mouse ESC. In addition, following the extended in vitro culture process, they maintained their expression of cell surface markers characteristic of the pluripotent stem cells such as alkaline phosphatase and Oct-4. When cultured without STO feeder cell layer and LIF, they were spontaneously differentiated into the various types of cells. Conclusion: The findings of this study suggest that the establishment of mouse ESC can be successfully derived from the in vitro fertilized embryos. The established ESC-like cells expressed the cell surface markers characteristic of the pluripotent stem cells and spontaneously differentiated into the various types of cells.

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.1141-1151
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• 2007

In this paper through Honam high-speed railroad which is planned with the north and south axis, we will verify the feasibility of the coordinate conversion using railroad control points after regarding current planned-railroad as the linear central axises. From analysis, distortion of Y axis varies 21cm to 40cm diminishing to a gentle straight line, distortion of X axis varies 14cm to 29cm. Through a revision, the deviation value between the coordinates were 6mm to 9mm and it satisfied the allowable error of national geographic information institute which is following ITRF (International Terrestrial Reference Frame) and cadastral boundary survey(10cm). consequently the coordinate conversion is possible using railroad control points as common control points.

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.480-493
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• 2007

Many railway-advanced countries are using the various types of track to reduce the track maintenance and repair cost according to the improvement of velocity. It spends on much maintenance and repair cost for ballast track due to abrasion of ballast, track irregularity and unisotropical ballast-support stiffness. The ballast track on railway bridge is accelerating the deterioration of ballast according to interaction of railway bridge and track. As continuing the deterioration, it is caused dynamic loads. Due to these effects, it increases negative loads of track and bridge. However, when designing the railway bridge, the effect of ballast track was applicate only dead load, so elastic behavior effect of ballast track is not influenced. Therefore, this paper presumes the stiffness of ballast track on railway bridge considering dynamic behavior of railway bridge, it was evaluated that effect on dynamic behaviors of railway bridge according to ballast track stiffness.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.3
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• pp.209-217
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• 2009

In this study, the antibacterial activity, antioxidative effects, inhibitory effects on tyrosinase of Inula britannica flower extracts were investigated. MIC values of ethyl acetate fraction from Inula britannica flower on P. acnes 0.25 %, respectively. The results showed that the antibacterial activity of the ethyl acetate fraction was the highest in the P. acnes. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities ($FSC_{50}$) of ethyl acetate fraction of Inula britannica flower was $8.55{\mu}g$/mL. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of some fInula britannica flower extracts on ROS generated in $Fe^{3+}$- EDTA/$H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activities were ethyl acetate fraction $0.24{\mu}g$/mL. Ethyl acetate fraction showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of Inula britannica flower on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The Inula britannica flower extracts suppressed photohemolysis in a concentration dependent manner ($5{\sim}100{\mu}g$/mL), particularly deglycosylated flavonoid aglycone fraction exhibited the most prominent celluar protective effect ($\tau_{50}$, 164.15 min at $25{\mu}g$/mL). The inhibitory effect of Inula britannica flower extracts on tyrosinase was investigated to assess their whitening efficacy. Inhibitory effects ($IC_{50}$) on tyrosinase of some Inula britannica flower extracts were high. Ethyl acetate fraction has $IC_{50}$ of $87.03{\mu}g$/mL. These results indicate that extract/fractions of Inula britannica flower can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. And inhibitory activity on tyrosinase of the ethyl acetate fraction and high potential as bactericide against the skin pathogenic bacteria could be applicable to new functional cosmetics for antioxidant, antiaging, antibacterial activity.