• Mass Spectrometry Letters
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• 제12권4호
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• pp.137-145
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• 2021

Herein we report multiple ion mobility (IM) peaks in electrospray ionization IM mass spectrometry (ESI-IM-MS) produced by glutamine residue in peptide. The mobility features of 147 peptides were investigated using ESI-IM-MS combined with liquid chromatography. Of these peptides, 66 presented multiple IM peaks, and analysis of their sequence using collision induced dissociation (CID) revealed that glutamine (Gln), as well as proline (Pro), plays a critical role in generating multiple IM peaks. Mutant-based investigations using Gln-containing peptides indicate that the side chain of Gln promotes intermolecular interactions, inducing multiple structures of the peptide ions in the gas phase. Consequently, the present study demonstrates that the distinct ion mobility signatures identified herein can potentially be used to characterize glutamine-containing peptide ions.

• Natural Product Sciences
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• 제26권4호
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• pp.334-339
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• 2020

A new compound, 3��-acetoxylanosta-7,9(11),24-triene-26-al (3), and seven known compounds (1 - 2 and 4 - 8) were isolated from Ganoderma tropicum (Jung.) Bres. collected in Tay Nguyen, Vietnam. The structures of these compounds were determined by one- and two-dimensional nuclear magnetic resonance spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and high-resolution ESI-MS, and by comparison with literature data. All of the isolated compounds were tested for nitroblue tetrazolium (NBT) reduction activity in Saccharomyces cerevisiae-stimulated RAW 246.7 cells. Among them, compounds 2 - 4 and 6 - 8 enhanced the NBT reduction in a dose-dependent manner.

• 한국식품과학회지
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• 제52권6호
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• pp.587-594
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• 2020

본 연구에서는 부위별 배추와 배추김치에 함유되어 있는 글루코시놀레이트류의 조성을 UPLC-MS/MS를 이용하여 탐색하고, 인돌류 대사체를 MS/MS를 이용하여 분석한 결과, 글루코시놀레이트류는 음이온 모드([M-H]-)에서 검출되었으며, glucobrassicanapin (m/z 386), glucoalyssin (m/z 450), glucobrassicin (m/z 447), 4-methoxyglucobrassicin (m/z 477), neoglucobrassicin (m/z 477)는 MS scan 모드에서, gluconapin (m/z 372→97), progoitrin (m/z 388→97), glucoiberin (m/z 422→97), 4-methoxyglucobrassicin (m/z 477→97), neoglucobrassicin (m/z 477→447)는 MS/MS MRM 모드에서 검출되었다. Glucobrassicin과 같은 인돌기 함유 글루코시놀레이트류들이 대사되어 생성되는 인돌류 대사체로는 ascorbigen (m/z 306→130)과 I3A (m/z 146→118)가 MS/MS MRM ([M+H]+) 모드에서 검출되었다. Ascorbigen은 NKC보다 IPC와 OPC에 유의적으로 많이 함유되어 있었다. I3A는 NKC에 가장 많이 함유되어 있었으나 시료 간 유의적인 차이는 없었다. 본 연구를 통하여 배추와 배추김치에 존재하는 글루코시놀레이트류와 그 대사체인 인돌류 물질을 UPLC-MS/MS를 이용하여 분석할 수 있었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.724-724
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• 2003

• Bulletin of the Korean Chemical Society
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• 제32권12호
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• pp.4415-4418
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• 2011

• Bulletin of the Korean Chemical Society
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• 제29권12호
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• pp.2465-2470
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• 2008

• Bulletin of the Korean Chemical Society
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• 제31권12호
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• pp.3517-3518
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• 2010

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 추계학술대회
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• pp.208-208
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• 2019

• 한국동물위생학회지
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• 제42권3호
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• pp.127-133
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• 2019

The purpose of this study was to investigate the contamination level of representative mycotoxins that have adverse effects on livestock by using LC-MS/MS method and to utilize the results as basic data for the establishment of quality control system for feed, and to provide information on production and storage. A total of nine mycotoxins, including aflatoxin $B_1$, aflatoxin $B_2$, aflatoxin $G_1$, aflatoxin $G_2$, ochratoxin A, fumonisin $B_1$, fumonisin $B_2$, deoxynivalenol (DON), zearalenone (ZEN) were simultaneously analyzed in LC-MS/MS under ESI positive mode. Fumonisin $B_1$ and fumonisin $B_2$ were detected from 3 cases of 75 forage produced in Incheon area, the detection rate was 4.0%. The detection concentration was 0.01~0.02 mg/kg, which was lower than the domestic recommended limit. Fumonisins were detected in a slightly different manner from the results of mycotoxin studies reported in Korea, which is attributed to the high temperature and dry summer weather of the year. The result of LC-MS/MS method performance of 9 mycotoxins, the recovery of DON was quite low as $41.53{\pm}3.91%$ that is not suitable for simultaneous analysis. This is probably due to that the extract solution used in this study was not suitable for the extraction of DON, along with the characteristics of a very dry forage. For the study of mycotoxins in Incheon area forage for the first time, further investigation is needed for the safe supply of livestock products.

• 분석과학
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• 제31권1호
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• pp.39-46
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• 2018

온라인 고체상 추출을 이용한 LC/MS/MS를 사용하여 carbaryl과 유기인계 농약 7 종을 동시에 분석하는 방법을 확립하였다. 분석 대상농약은 carbaryl, diazinon, methyl-demeton, fenitrothion, malathion, parathion, phenthoate, EPN 등 8 종이다. 본 연구에서는 온라인 고체상 추출과 LC/MS/MS의 ESI positive mode로 전처리 없이 물 시료 $500{\mu}L$를 직접 주입하여 10분안에 분석하였다. 농약 8 종의 회수율은 86.8~100 % 였고, 정확도는 90.6~98.8 %로 90 %이상이었고, 정밀도는 1.3~5.2 %로 나타났으며 검출한계 및 정량한계는 각각 $0.05{\sim}0.28{\mu}g/L$, $0.16{\sim}0.89{\mu}g/L$ 였다. $0.5{\mu}g/L$에서 $8.0{\mu}g/L$까지 다섯 개의 농도로 검량선을 작성한 결과 모든 물질의 상관계수(r2)가 0.99이상을 나타냈다. 방법의 유효성 평가를 한 결과 모든 내용에서 적합하므로확립한 분석방법으로 수질 시료에서 농약을 조사한 결과 모두 검출되지 않았다.