Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Studies on mycotoxins using LC-MS/MS for the forage produced in Incheon

  • Ra, Do Kyung (Incheon Metropolitan City Institute of Health & Environment) ;
  • Choi, Jae Yeon (Incheon Metropolitan City Institute of Health & Environment) ;
  • Lee, Ju Ho (Incheon Metropolitan City Institute of Health & Environment) ;
  • Nam, Ji Hyun (Incheon Metropolitan City Institute of Health & Environment) ;
  • Lee, Jeoung Gu (Incheon Metropolitan City Institute of Health & Environment) ;
  • Lee, Sung Mo (Incheon Metropolitan City Institute of Health & Environment)
  • Received : 2018.12.12
  • Accepted : 2019.07.02
  • Published : 2019.09.30
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Abstract

The purpose of this study was to investigate the contamination level of representative mycotoxins that have adverse effects on livestock by using LC-MS/MS method and to utilize the results as basic data for the establishment of quality control system for feed, and to provide information on production and storage. A total of nine mycotoxins, including aflatoxin $B_1$, aflatoxin $B_2$, aflatoxin $G_1$, aflatoxin $G_2$, ochratoxin A, fumonisin $B_1$, fumonisin $B_2$, deoxynivalenol (DON), zearalenone (ZEN) were simultaneously analyzed in LC-MS/MS under ESI positive mode. Fumonisin $B_1$ and fumonisin $B_2$ were detected from 3 cases of 75 forage produced in Incheon area, the detection rate was 4.0%. The detection concentration was 0.01~0.02 mg/kg, which was lower than the domestic recommended limit. Fumonisins were detected in a slightly different manner from the results of mycotoxin studies reported in Korea, which is attributed to the high temperature and dry summer weather of the year. The result of LC-MS/MS method performance of 9 mycotoxins, the recovery of DON was quite low as $41.53{\pm}3.91%$ that is not suitable for simultaneous analysis. This is probably due to that the extract solution used in this study was not suitable for the extraction of DON, along with the characteristics of a very dry forage. For the study of mycotoxins in Incheon area forage for the first time, further investigation is needed for the safe supply of livestock products.

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References

  1. Auerbach H. 2003. Mould growth and mycotoxin contamination of silage: sources, types and solution. pp. 247-265. In: Nyons TP, Jacques KA (ed.). Nutritional biotechnology in the feed and food industries. Nottingham Univ. Press, United Kingdom.
  2. Biomin. 2016. Mycotoxin survey 2016 third quarter (July-September). https://nutricionanimal.info/wp-content/uploads/2016/11/Mycotoxin-Survey-Presentation-Q3-2016-1.pdf
  3. CAST (Council for Agricultural Science & Technology). 1989. Mycotoxins: economics and health risks. Task Force Report No.116. Ames, Iowa.
  4. CAST (Council for Agricultural Science & Technology). 2003. Mycotoxins: lisks in plant, animal and human systems. Task Force Report No. 139. Ames, Iowa.
  5. D'Mello JPF, Placinta CM, Macdonald AMC. 1999. Fusarium mycotoxins: a review of global implications for animal health, welfare and productivity. Anim Feed Sci Tech 80: 183-205. https://doi.org/10.1016/S0377-8401(99)00059-0
  6. Fink-Gremmels J. 2005. Mycotoxin in forages. pp. 249-268. In: Diaz DE (ed.). The mycotoxin blue book. Nottingham Univ. Press, United Kingdom.
  7. Kim CJ, Jang JH. 1999. Food microbiology. Soohaksa, Seoul.
  8. Ministry of Agriculture, Food and Rural Affairs. 2014. Forage supply policy direction. pp. 4-23
  9. Ministry of Agriculture, Food and Rural Affairs. 2016. Control of livestock and fish feed act (notofication No.14481).
  10. Ministry of Agriculture, Food and Rural Affairs. 2017. Acceptance criteria from Standards for feed (notofication No.2017-28).
  11. National institute of agricultural sciences, Rural development administration. 2016. Understanding and prevention of mycotoxins
  12. National institute of food and drug safety evaluation. 2016. The method to analyze mycotoxins in food in 2016. pp. 61-63.
  13. Nelson CE. 1993. Strategies of mold control in dairy feeds. J Dairy Sci 76: 898-902. https://doi.org/10.3168/jds.S0022-0302(93)77416-0
  14. Nho WG, Yeo JM, Kim WY, Lee JH, Seo S, Kim MK, Seo GS. 2012. Taxonomy and Identification of fungi isolated from round bale silage. Journal of Practical Agriculture and Fisheries Research 14: 61-80.
  15. Santi DU, Sung HG, Lee CH, Lee SY, Kim SW, Cho KJ, Ha JK. 2009. Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats. L Vet Sci 10: 29-34. https://doi.org/10.4142/jvs.2009.10.1.29
  16. Schatzmayr G. 2005. Effective solution to control mycotoxins. Hoard's West. February(25): W42-W43.
  17. Scudamore KA, Livesey CT. 1998. Occurrence and significance of mycotoxins in forage crops and silage; a review. J Sci Food Agric 77: 1-17. https://doi.org/10.1002/(SICI)1097-0010(199805)77:1<1::AID-JSFA9>3.0.CO;2-4
  18. Sung HG. 2013. The studies on real condition of mycotoxin contamination in the fields before harvest and by the storage of rice straw using as roughage in Korea. J Kor Grassl Forage Sci 33(1): 21-29. https://doi.org/10.5333/KGFS.2013.33.1.21
  19. Sung HG, Kim SG, Lee JK. 2009. The presence of mycotoxigenic fungi in rice straw during the earlier spring of Korea. New paradigm for forage production in the East Asian region. Proceeding of the 3rd Korea-China-Japan joint symposium on Grassland Agriculture and Livestock Production. pp. 48-49.
  20. Sung HG, Lee JK, Seo S. 2011. Studies on fungal contamination and mycotoxins of rice straw round bale silage. J Kor Grassl Forage Sci 31: 451-462. https://doi.org/10.5333/KGFS.2011.31.4.451
  21. Sung HG, Lee JK, Seo S, Lim DC, Kim JD. 2010. Mold growth and mycotoxin contamination of forages. J Kor Grassl Forage Sci 30: 77-88. https://doi.org/10.5333/KGFS.2010.30.1.077