• Korean Journal of Exercise Nutrition
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• v.24 no.3
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• pp.13-18
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• 2020

[Purpose] In vivo studies have demonstrated the ergogenic benefits of eleutherococcus senticosus (ES) supplementation. ES has been observed to enhance endurance capacity, improve cardiovascular function, and alter metabolic functions (e.g., increased fat utilization); however, the exact mechanisms involved remain unknown. We aimed to determine whether ES could effectively induce fat loss and improve muscle metabolic profiles through increases in lipolysis- and lipid metabolism-associated protein expression in 3T3-L1 adipocytes and C2C12 skeletal muscle cells, respectively, to uncover the direct effects of ES on adipocytes and skeletal muscle cells. [Methods] Different doses of ES extracts (0.2, 0.5, and 1.0 mg/mL) were added to cells (0.2 ES, 0.5 ES, and 1.0 ES, respectively) for 72 h and compared to the vehicle control (control). [Results] The intracellular triacylglycerol (TG) content significantly decreased (p < 0.05 for 0.2 ES, p < 0.01 for 0.5 ES and 1.0 ES) in 3T3-L1 cells. Adipose triglyceride lipase, which is involved in active lipolysis, was significantly higher in the 1.0 ES group than in the control group (p < 0.01) of 3T3-L1 adipocytes. In C2C12 cells, the mitochondrial protein voltage-dependent anion channel (VDAC) was significantly increased in the 1.0 ES group (p < 0.01). Furthermore, we found that 1.0 ES activated both 5' AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in skeletal muscle cells (p < 0.01). [Conclusion] These findings suggest that ES extracts decreased TG content, presumably by increasing lipase in adipocytes and metabolism-associated protein expression as well as mitochondrial biogenesis in muscle cells. These effects may corroborate previous in vivo findings regarding the ergogenic effects of ES supplementation.

• Journal of the Korean Geographical Society
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• v.43 no.3
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• pp.312-323
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• 2008

The purpose of this paper is to analyze thermal sensation which is measured by human physioclimatic reactions in South Korea. Relationships between Temperature-Humidity Index(THI) and human thermal sensation scale are derived from a questionnaire, which investigates degree of volunteer's thermal sensation with respect to each biometeorological condition. Analyses of these empirical relationships make it possible to calculate thermal sensational indices and to classify bioclimatic types for individual weather stations based on long-term(1971-2000) averages of monthly temperature and humidity data. A generalized annual physioclimatic maps for each Annual Cumulative Thermal Sensation Index for the 68 stations are constructed to show men tend to feel in various areas. The Monthly thermal sensations are affected by latitude, altitude, orographic effects and systems of airmasses. The Annual Cumulative Thermal sensations are increasing towards northern areas and inland, and that the major factors are largely derived from cold stress in winter. The Annual Physioclimatic Types are grouped 8 climatic types(M, ES, M-ES, M-S, W-ES, C-ES, C-M, C-M-ES) according to climatic stress. Results of this study can be applied for evaluation of thermal environment in our daily activities, and for searching relevant sports training-sites, climatherapy etc.

• Reproductive and Developmental Biology
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• v.32 no.4
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• pp.223-227
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• 2008

Human embryonic stem (ES) cells retain the capacity for self-renewal, are pluripotent and differentiate into the three embryonic germ layer cells. The regulatory transcription factors Oct4, Nanog and Sox2 play an important role in maintaining the pluripotency of human ES cells. The aim of this research was to identify unknown genes upregulated in human ES cells along with Oct4, Nanog, and Sox2. This study characterizes an unknown gene, named chromosome 1 open reading frame 31 (C1orf31) mapping to chromosome 1q42.2. The product of C1orf31 is the hypothetical protein LOC388753 having a cytochrome c oxidase subunit VIb (COX6b) motif. In order to compare expression levels of C1orf31 in human ES cells, human embryoid body cells, vascular angiogenic progenitor cells (VAPCs), cord-blood endothelial progenitor cells (CB-EPCs) and somatic cell lines, we performed RT-PCR analysis. Interestingly, C1orf31 was highly expressed in human ES cells, cancer cell lines and SV40-immortalized cells. It has a similar expression pattern to the Oct4 gene in human ES cells and cancer cells. Also, the expression level of C1orf31 was shown to be upregulated in the S phase and early G2 phase of synchronized HeLa cells, leading us to purpose that it may be involved in the S/G2 transition process. For these reasons, we assume that C1orf31 may play a role in on differentiation of human ES cells and carcinogenesis.

• The Bulletin of The Korean Astronomical Society
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• v.46 no.2
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• pp.69.2-69.2
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• 2021

We present the discovery of rare active galactic nuclei (AGNs) in nearby (z<0.05) compact elliptical galaxies (cEs) located in isolated environments. Using spectroscopic data from the Sloan Digital Sky Survey (SDSS) Data Release 12, four AGNs were identified based on the optical emission-line diagnostic diagram. SDSS optical spectra of AGNs show the presence of distinct narrow-line emissions. Utilizing the black hole (BH) mass-stellar velocity dispersion scaling relation and the correlation between the narrow L([OIII])/L(Hβ) line ratio and the width of the broad Hα emission line, we estimated the BH masses of the cEs to be in the range of 7×10⁵-8×10⁷ solar mass. The observed surface brightness profiles of the cEs were fitted with a double Sérsic function using the Dark Energy Camera Legacy Survey r-band imaging data. Assuming the inner component as the bulge, the K-band bulge luminosity was also estimated from the corresponding Two Micron All Sky Survey images. We found that our cEs follow the observed BH mass-stellar velocity dispersion and BH mass-bulge luminosity scaling relations, albeit there was a large uncertainty in the derived BH mass of one cE. In view of the observational properties of BHs and those of the stellar populations of cEs, we discuss the proposition that cEs in isolated environments are bona fide low-mass early-type galaxies (i.e., a nature origin).

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.12
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• pp.1763-1768
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• 2001

The combined effects of low voltage electrical stimulation (ES) and early short-term temperature conditioning on meat quality of Hanwoo beef (Korean native cattle) during storage were investigated. Shear force was influenced by ES and aging. Combination of ES and the $30^{\circ}C$ conditioning resulted in higher myofibril fragmentation index and improved lightness. There was no substantial difference in drip loss among treatments but ES samples showed higher cooking loss than control. Negative effect on shelf-life was not found by early short-term high temperature conditioning. Therefore, the meat quality of Korean native cattle was effectively improved by the combination of ES and the $30^{\circ}C$ conditioning.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.2
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• pp.77.3-77.3
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• 2019

We present an analysis of the stellar population of compact elliptical galaxies (cEs) in various environments. Following conventional selection criteria of cEs, we created a list of cE candidates in the redshift range of z < 0.05 using SDSS DR12 catalog. We finally selected cEs with low-luminosity (Mg > 18.7 mag), small effective radius (Re < 600 pc), and high velocity dispersion (> 60 kms-1). We divide our cE sample into those inside and outside of the one virial radius of the bright (Mr < -21 mag) nearby host galaxy which is then defined as cEs with (cEw) and without (cEw/o) host galaxy, respectively. We investigated the stellar population properties of cEs based on the Hb, Mgb, Fe 5270, and Fe 5335 line strengths from the OSSY catalog. We found that cEw has a systematically higher metallicity than cEw/o. In the velocity dispersion-Mgb distribution, while cEw/o follows the relation of early-type galaxies, cEw are found to have a systematically higher metallicity than cEw/o at a given velocity dispersion. The different feature in the metallicity between cEw and cEw/o can suggest that two different scenarios can be provided in the formation of cEs. cEw would be the remnant cores of the massive progenitor galaxies that their outer parts have been tidally stripped by massive neighbor galaxies (i.e., nurture origin). On the other hand, cEw/o are likely to be faint-end of early-type galaxies maintaining in-situ evolution (i.e., nurture origin).

• Journal of the Korean Society of Tobacco Science
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• v.22 no.1
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• pp.76-83
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• 2000

This study was carried out to predict blending ratio of cut tobacco(CT), expanded stem(ES), and expanded cut tobacco(ECT) in cigarettes. CT, ES, and ECT samples from A brand were, ground and blended with reference to A blending ratio, and scanned by near infrared spectroscopy(NIRSystem Co., Model 6500). Calibration equations were developed and then determined blending ratio by NIRS. The standard error of calibration(SEC) and performance(SEP) of C factory samples between NIRS and known blending ratio were 0.97%, 1.93% for CT, 0.50%, 1.12 % for ES and 0.68%, 1.10% for ECT, respectively. The SEP of CT, ES and ECT of Band D factory samples determined by C factory calibration equation were more inaccurate than those of C factory samples determined by C factory calibration equations. These results were caused by the difference of CT, ES and ECT spectra followed by each factory. The SEP of CT, ES and ECT of Band D factories determined by calibration equations derived from each factory samples were more accurate than those of determined by calibration equation derived from C factory samples. Each factory SEP of CT, ES and ECT determined by calibration equation derived from all calibration samples(B+C+D factory) was similar to that determined by calibration equation derived from each factory samples. To improve the analytical inaccuracy caused by spectra difference, we need to apply a specific calibration equation for each factory sample. Data in development of specific calibrations between sample and NIRS spectra might supply a method for rapid determination of blending ratio of CT, ES, and ECT.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.176-181
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• 2011

Cronobacter sakazakii and Salmonella enterica Typhimurium are hazardous pathogens, especially for ready-toeat foods. For control of pathogens, the virulent bacteriophages were isolated, identified, and applied to infant formula milk and vegetable juice. The phages were isolated from swine feces and identified by morphology and molecular characteristics. ES2 phage for C. sakazakii and ST2 phage for S enterica Typhimurium were identified as Myoviridae and Siphoviridae, respectively. Their burst sizes were $52{\pm}5PFU/cell$ for ES2 phage and $21{\pm}3PFU/cell$ for ST2 phage after latent period of 30-40 minutes. ST2 phage showed higher heat stability at $60^{\circ}C$ than ES2 phage. ES2 phage held the growth of C. sakazakii untill 6 hr afterwhich the number decreased when applied to the infant formula milk and vegetable juice. ST2 phage also showed growth inhibition so that the number of S. enterica Typhimurium decreased. Therefore, virulent bacteriophages might be an agent for the growth inhibition of C. sakazakii and S. enterica Typhimurium in such the ready-to-eat foods.

• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.216-219
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• 2004

The effect of culture temperature on the production of soluble form of B. macerans cyclodextrin glucanotransferase (CGTase) in recombinant E. coli was investigated. E. coli cell was cotransformed with two plasmids (pTCGT1 and pGroll) in which the cgt and groEL/ES genes are under the control of T7 promoter and pzt-1 promoter, respectively. When tetracycline (10 ng/ml) and IPTG (l mM) were added as inducers at the early-exponential phase (2 h) and mid-exponential phase (3h), respectively, the solubilization of the inclusion body CGTase was greatly dependent on the temperature of the culture. At low culture temperature of $25^\circ{C}$, 2- or 3-fold higher activity and specific activity were obtained over $37^\circ{C}$. SDS-PAGE analysis revealed that about 62% of CGTase in the total CGTase protein was found in the soluble fraction by applying overexpression of GroEL/ES chaperone and by cultivation of E. coli at $25^\circ{C}$, whereas 33% of CGTase was detected in the soluble fraction at $37^\circ{C}$. Therefore, the expression of GroEL/ES and cultivation at $25^\circ{C}$ greatly enhanced the soluble production of CGTase in E. coli.

• Polymer(Korea)
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• v.26 no.4
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• pp.523-534
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• 2002

A new hydroxypropylcellulose (HPC) capable of exhibiting reflection colours in the temperature ranges of about 60-$130^{\circ}C$ and acrylic acid esters of HPC (ESs) with degree of esterification (DE) ranging from 1 to 3 were synthesized. The crosslinked ES films with the optical pitch ($\lambda_m$) ranging throughout the visible region were also prepared by exposing thermotropic cholesteric phases of ESs with a DE of more than 2 to UV light at $50^{\circ}C$. The thermal and optical properties for both the uncrosslinked and crosslinked samples and the swelling behavior of the crosslinked films in acetone were investigated. The $\lambda_m$'s of ESs, as well as HPC itself, increased with temperature. However, the $\lambda_m$'s of ESs were larger than of HPC at the same temperature and decreased with increasing DE. The temperature dependence of $\lambda_m$of the crosslinked samples was much weaker than that of ESs. Moreover, in contrast with ESs that exhibit a decrease of the isotropization temperature with increase in the DE, the networks were found to decompose at about $210^{\circ}C$, giving no transition to an isotropic state. The crosslinked samples exhibited an anisotropic swelling, suggesting that the two-dimensional crosslinking preferentially performs between ES molecules.