• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.19-19
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• 2002

This study was to examine whether the in vitro differentiated neural cells derived from human embryonic stem (hES, MB03) cells can be survived and expressed tyrosin hydroxylase(TH) in grafted normal or PD rat brain. To differentiate in vitro into neural cells, embryoid bodies (EB: for 5 days, without mitogen) were formed from hES cells, neural progenitor cells(neurosphere, for 7-10 days, 20 ng/㎖ of bFGF added N2 medium) were produced from EB, and then finally neurospheres were differentiated into mature neuron cells in N2 medium(without bFGF) for 2 weeks. In normal rat brain, neural progenitor cells or mature neuron cells (1×10/sup 7/ cells/㎖) were grafted to the striatum of normal rats. After 2 weeks, when the survival of grafted hES cells was examined by immunohistochemical analysis, the neural progenitor cell group indicated higher BrdU, NeuN＋, MAP2＋ and GFAP＋ than mature neuron cell group in grafted sites of normal rats. This result demonstrated that the in vivo differentiation of grafted hES cells be increased simultaneously in both of neuronal and glial cell type. Also, neural progenitor cell grafted normal rats expressed more TH pattern than mature neuron cells. Based on this data, as a preliminary test, when the neural progenitor cells were grafted into the striatum of 6-hydroxydopamine lesioned PD rats, we confirmed the cell survival (by double staining of Nissl and NeuN) and TH expression. This result suggested that in vitro differentiated neural progenitor cells derived from hES cells are more usable than mature neuron cells for the neural cell grafting in animal model and those grafted cells were survived and expressed TH in normal or PD rat brain.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.27 no.3
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• pp.84-95
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• 2014

Objective : Ephedra sinica (ES) has has been used as remedy of allergic diseases for a long time in Korea. In the present study, we investigated the anti-allergic effects of ES on experimental allergic asthma mouse model using ovalbumin (OVA). Methods : BALB/c mice were sensitized and challenged with 100 ug of OVA and 1 mg of aluminum potassium sulfate of 0.2 ml phosphate-buffered saline(PBS) intraperitoneally on day 1 and 15. mice were challenged on 3 consecutive days with 5% OVA and AHR was assessed 24 h after the last challenge. we examined the lung histology, airway hyper sensitivity, total inflammatory cell count in bronchoaveloar lavage fluid(BALF), Th2-associated cytokines production and IgE production. Results : ES potently inhibited the lung damage and the development of Penh. ES also reduced the number of BAL cells during OVA-induced allergic asthma. Furthermore, ES inhibited cytokines production such as IL-4, IL-13 productions, and IgE level of serum. Conclusion : These results suggest that ES may inhibit the production of IL-4, IL-13, IgE and infiltration of inflammatory cell and be beneficial oriental medicine for allergic asthma.

• Food Science and Preservation
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• v.24 no.2
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• pp.320-324
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• 2017

Eruca sativa is a rocket plant and a member of the Brassicaceae, which is considered to be an important chemo-preventive plant family. Although Eruca sativa has positive biological effects, the effect of Eruca sativa extract (ES) on improvement of skin barrier function has not been reported. In this study, we investigated the applicability of functional materials by examining a variety of physiological activities of Eruca sativa extract. ES showed anti-microbial activities against Bacillus subtilis, Escherichia coli, and Candida albicans. In particular, antimicrobial activities of ES against B. subtilis was the highest. Additionally, immunohistochemical analysis of protein marker related to keratinocyte differentiation was determined. The treatment by ES (50 mg/L) showed a significant increase of involucrin expression compared with treatment by 0.1% DMSO as a control in skin equivalents, the ES-treated group showed similar level in the expression of involucrin compared to the group treated with the same concentration of WY14643 in $EpiDerm^{TM}$, a three-dimensional model of skin equivalents. These results indicate that ES promotes the expression of protein related to barrier properties of the skin. Therefore, ES may be an effective ingredient for skin barrier improvement.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.12 no.1
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• pp.57-63
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• 2012

TIP technology enables navigation of the internal contents of images by extracting geometry information from two-dimensional drawing or a picture and generating three-dimensional effects from extracted information. The technology can be applied to a variety of practical fields including game, entertainment, education, public relations and so on. This paper proposes extended application of TIP technology and realization method for smart devices using OpenGL ES Library for Android platform. Considering problems associated with a foreground object extraction, the proposed method uses vanishing points chosen by the user to facilitate more realistic scene configuration. Then, method acquires three-dimensional background model using OpenGL ES Library, develops three-dimensional virtual space and enables image navigation via camera viewpoint conversion. The experimental image is made on Android 2.1 and OpenGL ES 1.0 using the image taken on devices built on the Android platform. Thus, the proposed technology can be implemented to various smart devices built on the Android platform at lower cost and in less time.

• Membrane and Water Treatment
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• v.11 no.2
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• pp.123-130
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• 2020

Submerged Membrane bioreactor (SMBR) is one of the last techniques that allow a high quality of treated industrial effluents by coupling biological treatment and membrane separation. Thus, this research was an effort to evaluate performance of a SMBR treating a model textile wastewater (MTWW). Different SMBR operating parameters like mixed liquor suspended solids (MLSS) and Dissolved oxygen concentration, hydraulic retention time (HRT), and nutrients addition (N and P) have been investigated. MTWW (influent to the SMBR) was generated using the reactive azo-dye, Novacron blue FNG (100mg/L feed concentration). Results of MTWW treatment using SMBR under optimal operating conditions (MLSS, 4.2-13.3g/L; HRT, 4 days; pH, 6.9-7.2; conductivity, 400-900 μS/cm and temperature, 19.4-22.2 ℃) showed that COD and blue colour treatment performances are between 94-98% and 30-80%, respectively. It is concluded that SMBR can be used in large scale textile wastewater treatment plants to improve effluent quality in order to meet effluent discharge standards.

• Bulletin of the Korean Space Science Society
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• 2003.10a
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• pp.82-82
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• 2003

It is well known that electromagnetic (EM) waves are mode converted to electrostatic (ES) waves in inhomogeneous plasmas. We examine this issue in a three-dimensional multi-fluid numerical model. First, we derive a set of coupled linear wave equations when a one-dimensional inhomogeneous density profile is assumed in a cold and collisionless plasma. The massive ions are considered as fixed because we are interested in high frequency waves in plasmas. It is shown that the EM mode satisfies the 0th order modified Bessel equation near the resonant region where the frequency matches the local electron plasma frequency. It is expected that the EM waves are coupled and damped to the ES waves owing to the logarithmic singular behavior at such resonances. Second, we numerically test the same case in a 3-D multi-fluid model. An impulsive input is assumed to excite EM waves in the inhomogeneous 3-D box model. The wave spectra of electric and magnetic fields are presented and compared with the analytical results. Our results suggest that the EM energy is irreversibly converted into the ES energy wherever the resonant condition is satisfied. Finally we discuss how the mode conversion appears in both electric and magnetic fields by analyzing time histories of each component. We also compare our results with MHD wave coupling. It is numerically confirmed in this study that the coupling of EM and ES waves is similar to that of compressional and transverse MHD waves.

• Geomechanics and Engineering
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• v.29 no.1
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• pp.91-97
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• 2022

The cohesive non-swelling soil (CNS) cushion technology has been widely applied in the subgrade and slope improvement at expansive soil regions. However, the mechanism of the inhibition effect of the CNS layer on expansive soil (ES) has not been fully understood. We performed four outdoor model tests to further understand the inhibition effect, including different kinds of upper layer and thickness, under the unidirectional seepage condition. The swelling deformation, soil pressure, and electrical resistivity were constantly monitored during the saturation process. It is found that when a CNS layer covered the ES layer, the swelling deformation and electrical resistivity of the ES layer decreased significantly, especially the upper part. The inhibition effect of the CNS layer increases with the increase of CNS thickness. The distribution of vertical and lateral soil pressure also changed with the covering of a CNS layer. The electrical resistivity can be an effective index to describe the swelling deformation of ES layer and analyze the inhibition effect of the CNS layer. Overall, the CNS deadweight and the ion migration are the major factors that inhibit the swelling deformation of expansive soil.

• Journal of the Korean Society of Visualization
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• v.17 no.2
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• pp.32-38
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• 2019

A comparison study between computational-fluid-dynamics simulation and wind tunnel test for a megawatt-class wind turbine is conducted. For the study, flow-field in wake, basic aerodynamic performance, and effect of the yaw error for a 1/86 scaled-down model of the NREL offshore 5 MW wind turbine are numerically calculated using commercial software "FloEFD" with $k-{\varepsilon}$ turbulence model. The computed results are compared to the wind tunnel test performed by the constant-velocity mode for the model. It is shown that discrepancy are found between the two results at lower tip-speed ratio and higher yaw angle, however, the velocity-defection distribution in the wake, the torque coefficient at moderated and high tip-speed ratios are in good agreement with the wind tunnel test.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.2
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• pp.133-139
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• 2018

Keeping a proper straight route length under the marine bridge is one of the important factors for the safe passage of ships. However, according to port and harbor design standards, there is only a constant guideline of 8 times the length of the marine bridge underpass. On this study, we used the ES model to determine the ratio of risk to the route width, traffic volume, the degree of curvature of the route, and the length of the straight route in order to derive the optimal straight route distance. As a result, the risk ratio decreased by 2.27% as the route distance increased from 3L to 10L when the degree of curvature of the route was $45^{\circ}$. The risk associated with curvature was found to be 4.83% when the bending degree was changed from $0^{\circ}$ to $45^{\circ}$ in the case of 3L length. In addition, it was confirmed that the risk ratio according to the degree of curvature of the route and the straight route was reduced by 1.45% at maximum under the condition that the width of the line was 400m and the number of the vessels generated per hour was 20. It was verified that a straight route distance more than a certain length is needed depending on the congestion degree and the degree of curvature of the route when constructing the marine bridge.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.12
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• pp.1705-1710
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• 2003

A small body size of Brown Tsaiya laying duck is desirable to reduce maintenance requirements, so the body weight at 40 weeks of age (BW40) has to be maintained at its current level. Egg weight has to be maintained at around 65 g to meet market requirements. Eggshell strength at 40 weeks of age (ES40) must to be increased in order to maintain a low incidence of broken eggs. Thus, number of eggs laid up to 52 weeks of age (EN52) has to be increased without negative correlated response on ES40. A new linear genetic selection index was used: $I_g=a_0{\times}GEW40\;(g)+a_1{\times}GBW40\;(g)+a_2{\times}GES40\;(kg/cm^2)+a_3{\times}GEN52\;(eggs)$ where GEW40, GBW40, GES40 and GEN52 were the multitrait best linear unbiased prediction (MT-BLUP) animal model predictors of the breeding values respectively of egg weight and body weight at 40 weeks of age (EW40, BW40), ES40 and EN52. The coefficients $a_0$, $a_1$, $a_2$ and $a_3$ were calculated with constraints of 0.0 g, 0.0 g and $0.013kg/cm^2$ for expected genetic gains in EW40, BW40 and ES40 respectively and maximum gain in EN52. Since 1997, the drakes and the ducks were selected according to their own indexes, with this new genetic selection index. From G0 to G4, the average per generation predicted genetic responses in female duck were +0.05 g for EW40, +0.92 g for BW40, $+0.035kg/cm^2$ for ES40 and +2.13 eggs for EN52. Which represented respectively 0.07%, 0.06%, 0.67% and 1.0% of the means of the EW40, BW40, ES40 and EN52. For ES40 and EN52, it represented also respectively 16.1% and 21.6% of the additive genetic standard deviation of these traits. Thevse results indicated that selection of laying Brown Tsaiya by a restricted genetic selection index and with MT-BLUP animal model could be an efficient tool for improving the efficiency of egg production, increasing egg shell strength and egg number while holding egg weight and body weight constants.