• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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• pp.19-19
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• 2002

This study was to examine whether the in vitro differentiated neural cells derived from human embryonic stem (hES, MB03) cells can be survived and expressed tyrosin hydroxylase(TH) in grafted normal or PD rat brain. To differentiate in vitro into neural cells, embryoid bodies (EB: for 5 days, without mitogen) were formed from hES cells, neural progenitor cells(neurosphere, for 7-10 days, 20 ng/㎖ of bFGF added N2 medium) were produced from EB, and then finally neurospheres were differentiated into mature neuron cells in N2 medium(without bFGF) for 2 weeks. In normal rat brain, neural progenitor cells or mature neuron cells (1×10/sup 7/ cells/㎖) were grafted to the striatum of normal rats. After 2 weeks, when the survival of grafted hES cells was examined by immunohistochemical analysis, the neural progenitor cell group indicated higher BrdU, NeuN＋, MAP2＋ and GFAP＋ than mature neuron cell group in grafted sites of normal rats. This result demonstrated that the in vivo differentiation of grafted hES cells be increased simultaneously in both of neuronal and glial cell type. Also, neural progenitor cell grafted normal rats expressed more TH pattern than mature neuron cells. Based on this data, as a preliminary test, when the neural progenitor cells were grafted into the striatum of 6-hydroxydopamine lesioned PD rats, we confirmed the cell survival (by double staining of Nissl and NeuN) and TH expression. This result suggested that in vitro differentiated neural progenitor cells derived from hES cells are more usable than mature neuron cells for the neural cell grafting in animal model and those grafted cells were survived and expressed TH in normal or PD rat brain.

• 한방안이비인후피부과학회지
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• 제27권3호
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• pp.84-95
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• 2014

Objective : Ephedra sinica (ES) has has been used as remedy of allergic diseases for a long time in Korea. In the present study, we investigated the anti-allergic effects of ES on experimental allergic asthma mouse model using ovalbumin (OVA). Methods : BALB/c mice were sensitized and challenged with 100 ug of OVA and 1 mg of aluminum potassium sulfate of 0.2 ml phosphate-buffered saline(PBS) intraperitoneally on day 1 and 15. mice were challenged on 3 consecutive days with 5% OVA and AHR was assessed 24 h after the last challenge. we examined the lung histology, airway hyper sensitivity, total inflammatory cell count in bronchoaveloar lavage fluid(BALF), Th2-associated cytokines production and IgE production. Results : ES potently inhibited the lung damage and the development of Penh. ES also reduced the number of BAL cells during OVA-induced allergic asthma. Furthermore, ES inhibited cytokines production such as IL-4, IL-13 productions, and IgE level of serum. Conclusion : These results suggest that ES may inhibit the production of IL-4, IL-13, IgE and infiltration of inflammatory cell and be beneficial oriental medicine for allergic asthma.

• 한국식품저장유통학회지
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• 제24권2호
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• pp.320-324
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• 2017

본 연구에서 루꼴라(Eruca sativa)에 대한 다양한 생리활성을 조사하여 기능성소재 응용가능성을 검토하였다. 루꼴라 추출물은 B. subtilis, E. coli, C. albicans에 대한 항균활성이 매우 우수하게 나타났으며 특히 그람양성세균 B. subtilis에 대한 항균활성이 높았다. 또한, 인볼루크린 발현에 대한 면역조직화학 분석을 인공피부를 이용하여 분석한 결과 루꼴라 추출물을 처리(50 mg/L)한 경우에 0.1% DMSO로 처리한 대조군에 비하여 인볼루크린 발현이 뚜렷하게 증가하였으며 동일농도로 처리된 합성물질 WY14643과 비교하였을 때에도 비슷한 발현양상을 보여 주었다. 이와 같은 결과로 미루어 볼 때 루꼴라 추출물은 피부염 개선 및 피부장벽 기능을 향상할 수 있는 천연물 기반의 소재로 활용하기 위한 매우 효과적인 재료가 될 수 있다고 사료된다.

• 한국인터넷방송통신학회논문지
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• 제12권1호
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• pp.57-63
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• 2012

TIP기술은 2차원 그림 또는 한 장의 사진으로부터 기하정보를 추출하여 3차원 입체 효과를 만들어 영상 내부를 내비게이션 할 수 있는 기술로써, 게임, 엔터테인먼트, 교육, 홍보 등 다양한 응용분야에서 활용 가능한 기술이다. 본 논문에서는 android platform상에서의 OpenGL ES Library를 이용하여 확장된 TIP기술 적용 및 구현 방법을 제안한다. 제안 방법은 전경객체의 추출이 어려운 상황을 감안하여 보다 사실적 장면 구성이 용이하도록 사용자의 선택에 의한 소실점을 이용하고, OpenGL ES Library 를 이용하여 3차원 배경 모델을 획득한 후 이미지를 텍스처 매핑하여 3차원 가상공간을 완성한 후 카메라의 시점변환을 통해 이미지 내부를 내비게이션이 가능하도록 한다. 실험영상은 android platform상의 device에서 촬영한 이미지를 사용하여 android 2.1 및 OpenGL ES 1.0기반으로 구축된 제안 기술로 android platform의 스마트 device에서 적은 비용과 시간으로 다양한 응용개발에 적용 가능하도록 구현하였다.

• Membrane and Water Treatment
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• 제11권2호
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• pp.123-130
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• 2020

Submerged Membrane bioreactor (SMBR) is one of the last techniques that allow a high quality of treated industrial effluents by coupling biological treatment and membrane separation. Thus, this research was an effort to evaluate performance of a SMBR treating a model textile wastewater (MTWW). Different SMBR operating parameters like mixed liquor suspended solids (MLSS) and Dissolved oxygen concentration, hydraulic retention time (HRT), and nutrients addition (N and P) have been investigated. MTWW (influent to the SMBR) was generated using the reactive azo-dye, Novacron blue FNG (100mg/L feed concentration). Results of MTWW treatment using SMBR under optimal operating conditions (MLSS, 4.2-13.3g/L; HRT, 4 days; pH, 6.9-7.2; conductivity, 400-900 μS/cm and temperature, 19.4-22.2 ℃) showed that COD and blue colour treatment performances are between 94-98% and 30-80%, respectively. It is concluded that SMBR can be used in large scale textile wastewater treatment plants to improve effluent quality in order to meet effluent discharge standards.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2003년도 한국우주과학회보 제12권2호
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• pp.82-82
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• 2003

It is well known that electromagnetic (EM) waves are mode converted to electrostatic (ES) waves in inhomogeneous plasmas. We examine this issue in a three-dimensional multi-fluid numerical model. First, we derive a set of coupled linear wave equations when a one-dimensional inhomogeneous density profile is assumed in a cold and collisionless plasma. The massive ions are considered as fixed because we are interested in high frequency waves in plasmas. It is shown that the EM mode satisfies the 0th order modified Bessel equation near the resonant region where the frequency matches the local electron plasma frequency. It is expected that the EM waves are coupled and damped to the ES waves owing to the logarithmic singular behavior at such resonances. Second, we numerically test the same case in a 3-D multi-fluid model. An impulsive input is assumed to excite EM waves in the inhomogeneous 3-D box model. The wave spectra of electric and magnetic fields are presented and compared with the analytical results. Our results suggest that the EM energy is irreversibly converted into the ES energy wherever the resonant condition is satisfied. Finally we discuss how the mode conversion appears in both electric and magnetic fields by analyzing time histories of each component. We also compare our results with MHD wave coupling. It is numerically confirmed in this study that the coupling of EM and ES waves is similar to that of compressional and transverse MHD waves.

• Geomechanics and Engineering
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• 제29권1호
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• pp.91-97
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• 2022

The cohesive non-swelling soil (CNS) cushion technology has been widely applied in the subgrade and slope improvement at expansive soil regions. However, the mechanism of the inhibition effect of the CNS layer on expansive soil (ES) has not been fully understood. We performed four outdoor model tests to further understand the inhibition effect, including different kinds of upper layer and thickness, under the unidirectional seepage condition. The swelling deformation, soil pressure, and electrical resistivity were constantly monitored during the saturation process. It is found that when a CNS layer covered the ES layer, the swelling deformation and electrical resistivity of the ES layer decreased significantly, especially the upper part. The inhibition effect of the CNS layer increases with the increase of CNS thickness. The distribution of vertical and lateral soil pressure also changed with the covering of a CNS layer. The electrical resistivity can be an effective index to describe the swelling deformation of ES layer and analyze the inhibition effect of the CNS layer. Overall, the CNS deadweight and the ion migration are the major factors that inhibit the swelling deformation of expansive soil.

• 한국가시화정보학회지
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• 제17권2호
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• pp.32-38
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• 2019

A comparison study between computational-fluid-dynamics simulation and wind tunnel test for a megawatt-class wind turbine is conducted. For the study, flow-field in wake, basic aerodynamic performance, and effect of the yaw error for a 1/86 scaled-down model of the NREL offshore 5 MW wind turbine are numerically calculated using commercial software "FloEFD" with $k-{\varepsilon}$ turbulence model. The computed results are compared to the wind tunnel test performed by the constant-velocity mode for the model. It is shown that discrepancy are found between the two results at lower tip-speed ratio and higher yaw angle, however, the velocity-defection distribution in the wake, the torque coefficient at moderated and high tip-speed ratios are in good agreement with the wind tunnel test.

• 해양환경안전학회지
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• 제24권2호
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• pp.133-139
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• 2018

해상교량 하부의 직선항로 길이 확보는 선박 통항 안전을 위한 중요한 요소 중 하나이다. 그러나 항만 및 어항 설계기준에 따르면 해상교량 하부 직선항로 길이는 선박길이의 8배로 획일적인 가이드라인을 적용하고 있다. 본 연구는 적정 해상교량 하부직선길이를 도출하기 위해 ES 모델을 이용하여 항로폭, 통항량, 항로의 곡률, 직선항로길이에 따른 위험도 비율을 확인했다. 확인 결과 항로의 곡률이 $45^{\circ}$의 경우 항로길이가 3L에서 10L로 길어짐에 따라 위험도 비율이 2.27 % 감소했다. 곡률에 따른 위험도는 직선항로의 길이가 3L의 경우 곡률이 $45^{\circ}$에서 $0^{\circ}$로 변하면서 위험도 비율이 4.83 % 감소하는 것을 확인했다. 또한 항로폭 400 m, 시간당 발생선박이 20척의 조건에서 항로의 곡률별, 직선항로에 따른 위험도 비율은 최대 1.45 % 감소하는 것을 확인했다. 이를 통해 해상 교량 건설 시 항로의 혼잡도 및 곡률에 따라 일정 길이 이상의 직선항로가 필요함을 검증했다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권12호
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• pp.1705-1710
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• 2003

A small body size of Brown Tsaiya laying duck is desirable to reduce maintenance requirements, so the body weight at 40 weeks of age (BW40) has to be maintained at its current level. Egg weight has to be maintained at around 65 g to meet market requirements. Eggshell strength at 40 weeks of age (ES40) must to be increased in order to maintain a low incidence of broken eggs. Thus, number of eggs laid up to 52 weeks of age (EN52) has to be increased without negative correlated response on ES40. A new linear genetic selection index was used: $I_g=a_0{\times}GEW40\;(g)+a_1{\times}GBW40\;(g)+a_2{\times}GES40\;(kg/cm^2)+a_3{\times}GEN52\;(eggs)$ where GEW40, GBW40, GES40 and GEN52 were the multitrait best linear unbiased prediction (MT-BLUP) animal model predictors of the breeding values respectively of egg weight and body weight at 40 weeks of age (EW40, BW40), ES40 and EN52. The coefficients $a_0$, $a_1$, $a_2$ and $a_3$ were calculated with constraints of 0.0 g, 0.0 g and $0.013kg/cm^2$ for expected genetic gains in EW40, BW40 and ES40 respectively and maximum gain in EN52. Since 1997, the drakes and the ducks were selected according to their own indexes, with this new genetic selection index. From G0 to G4, the average per generation predicted genetic responses in female duck were +0.05 g for EW40, +0.92 g for BW40, $+0.035kg/cm^2$ for ES40 and +2.13 eggs for EN52. Which represented respectively 0.07%, 0.06%, 0.67% and 1.0% of the means of the EW40, BW40, ES40 and EN52. For ES40 and EN52, it represented also respectively 16.1% and 21.6% of the additive genetic standard deviation of these traits. Thevse results indicated that selection of laying Brown Tsaiya by a restricted genetic selection index and with MT-BLUP animal model could be an efficient tool for improving the efficiency of egg production, increasing egg shell strength and egg number while holding egg weight and body weight constants.