• Korean Journal of Environmental Agriculture
• /
• v.30 no.3
• /
• pp.323-329
• /
• 2011

BACKGROUND: The conazoles, difenoconazole, diniconazole, hexaconazole, penconazole and tetraconazole are a large class of synthetic fungicides used extensively for foliage and seed treatments in agricultural crops. The extensive use of conazoles has brought concerns on the potentiality of environmental contamination and toxicity. Thus studies on the development of methods for monitoring the conazoles are required. METHODS AND RESULTS: A modified quick, easy, effective, rugged and safe (QuEChERS) method was involved in sample preparation. Quadrapole time of flight mass spectrometer (Q-TOF MS) in electron spray ionization (ESI) mode was employed to determine conazoles in garlic samples. The limit of detection (LOD) and limit of quantification (LOQ) of conazoles by Q-TOF-MS ranged from 0.001 to 0.002 mg/L and 0.002 to 0.005 mg/L, respectively. Q-TOF-MS analysis exhibited less than 2.6 ppm error of accurate mass measurements for the detection of conazoles spiked at 0.05 mg/L in garlic matrix. Recovery values of conazoles fortified in garlic samples at 0.02, 0.05 and 0.1 mg/L were between 79.2 and 106.2% with a maximum 11.8% of standard deviation. No detectable conazoles were found in the domestic market samples by using the Q-TOF-MS method. CONCLUSION(s): High degree of confirmation for conazoles by accurate mass measurements demonstrated that Q-TOF-MS analysis combined with a QuEChERS method may be applicable to simultaneous determination of conazoles in garlic samples.

• Korean Journal of Remote Sensing
• /
• v.19 no.4
• /
• pp.271-276
• /
• 2003

Sea level variability and eddy in the Amsterdam-Crozet-Kerguelen region of the South Indian Ocean were studied during 1 year (October 15, 1992~October 15, 1993) using multi-satellite data (Topex/Poseidon, ERS1) produced by signal treatment. We found that sea level variabilities (>10cm) generally exist along the Antarctic Circumpolar Current in the area of 35$^{\circ}$~46$^{\circ}$S and strong sea level variabilities (20~30cm) were especially occurred from the effects of eddy due to bottom topography in two small area: 49$^{\circ}$~57$^{\circ}$E and 38$^{\circ}$~42$^{\circ}$S, and 58$^{\circ}$~64$^{\circ}$E and 42$^{\circ}$~44$^{\circ}$S.

• Korean Journal of Environmental Agriculture
• /
• v.42 no.3
• /
• pp.220-230
• /
• 2023

Imidacloprid is a neonicotinoid insecticide widely used for insect control in a variety of crops. The evaluation of imidacloprid total residues in animal feeds derived from crop by-products is required to ensure the safety of livestock products. We performed simultaneous LC/MS/MS analyses of imidacloprid and its metabolites in five different livestock products including beef, pork, chicken, milk and egg from domestic markets. The methods for sample preparation and instrumental analysis were established by modifying QuEChERS method to meet the Codex guidelines. The methods generated 0.0035 mg/kg of the limit of determination (LOD), 0.01 mg/kg of the limit of quantitation (LOQ) and standard calibration linearity with >0.983 of the coefficients of determination (R²). The methods exhibited the recovery values of imidacloprid and its metabolites ranging from 65.66 to 119.27% without any interference between matrices. Imidacloprid total residues in the livestock products were found as values lower than the LOQ and maximum residue limits (MRLs). This study suggests that the methods are successfully applicable for the safety evaluation of imidacloprid total residues in livestock products from domestic markets.

• Korean Journal of Clinical Laboratory Science
• /
• v.55 no.4
• /
• pp.276-283
• /
• 2023

The Occupational Safety and Health Research Institute is currently evaluating spirometry tests used for worker health examinations by applying the 2005 American Thoracic Society (ATS) and European Respiratory Society (ERS) spirometric test standardization guide and reviewing the application of the 2019 ATS/ERS guide. To compare results obtained using the new evaluation criteria with previous results and determine whether it is appropriate to apply them to Korean workers' health examinations, we reviewed spirometry results from 325 special health examination institutions. Although evaluation criteria such as extrapolation volume, correction error, and forced inspiratory vital capacity were applied more strictly, institutions had higher reliability scores. Primarily because the acceptability and repeatability of forced expiratory volume in 1 second and forced vital capacity were judged separately, and thus, deduction width was reduced. The study shows that adopting the new evaluation criteria would reduce the possible use of inappropriate data, increase tester and doctor understanding of result selection and interpretation, increase result reliability, and reduce the testing burden.

• The Korean Journal of Physiology and Pharmacology
• /
• v.28 no.2
• /
• pp.129-143
• /
• 2024

Sulfur dioxide (SO₂), a novel endogenous gas signaling molecule, is involved in the regulation of cardiac function. Exerting a key role in progression of hyperthyroidism-induced cardiomyopathy (HTC), myocardial fibrosis is mainly caused by myocardial apoptosis, leading to poor treatment outcomes and prognoses. This study aimed to investigate the effect of SO₂ on the hyperthyroidism-induced myocardial fibrosis and the underlying regulatory mechanisms. Elisa, Masson staining, Western-Blot, transmission electron microscope, and immunofluorescence were employed to evaluate the myocardial interstitial collagen deposition, endoplasmic reticulum stress (ERS), apoptosis, changes in endogenous SO₂, and Hippo pathways from in vitro and in vivo experiments. The study results indicated that the hyperthyroidism-induced myocardial fibrosis was accompanied by decreased cardiac function, and down-regulated ERS, apoptosis, and endogenous SO₂-producing enzyme aspartate aminotransferase (AAT)1/2 in cardiac myocytes. In contrast, exogenous SO₂ donors improved cardiac function, reduced myocardial interstitial collagen deposition, up-regulated AAT1/2, antagonized ERS and apoptosis, and inhibited excessive activation of Hippo pathway in hyperthyroid rats. In conclusion, the results herein suggested that SO₂ inhibited the overactivation of the Hippo pathway, antagonized ERS and apoptosis, and alleviated myocardial fibrosis in hyperthyroid rats. Therefore, this study was expected to identify intervention targets and new strategies for prevention and treatment of HTC.

• The Korean Journal of Pesticide Science
• /
• v.17 no.4
• /
• pp.264-270
• /
• 2013

Imidacloprid is a systemic insecticide which act as an insect neurotoxin. It used for control of pest such as aphids and other sucking insects in fruits and vegetables. Systemic pesticides move inside a crop following absorption by the plant, and these were converted into a variety of metabolites. Sometimes these metabolites make a problem about safety of agricultural products. So a simultaneous determination method of pesticide and its metabolites is needed, to monitor their presence in agricultural product and study on the fate of pesticide in a plant. This study's aim is to investigate simultaneous analysis method of imidacloprid and its metabolites, imidacloprid guanidine, imidacloprid olefin, imidacloprid urea, and 6-chloronicotinic acid in red pepper using QuEChERS method and LC-MS/MS systems. QuEChERS method was modifed beacuase $MgSO_4$ salts decreased the recoveries of 6-chloronicotinic acid in extraction procedure. Imidacloprid and its metabolites were extracted by acetonitrile with 1% glacial acetic acid and the extracts were purified through QuEChERS with primary secondary amine (PSA) and $C_{18}$ and analyzed with LC-MS/MS in ESI positive mode. Standard calibration curves were made by matrix matched standards and their correlation coefficients were higher than 0.999. Recovery studies were carried out on spiked pepper blank sample at four concentration levels (0.01, 0.04 and 0.1, 0.4 mg/kg). The average recoveries of imidacloprid and its metabolites were in the range of 70~120% with < 20% RSD. This result indicated that the method using QuEChERS and LC-MS/MS was suitable for the simultaneous determination of imidacloprid and its metabolites in red pepper.

• The Korean Journal of Pesticide Science
• /
• v.15 no.4
• /
• pp.401-416
• /
• 2011

The recent trend for pesticide residue analysis in food involves fast cleanup and use of mass spectrometry to achieve quantitative and qualitative analysis at the same time. Recently, the QuEChERS (quick, easy, cheap, effective, rugged and safe) multi-reside method has received much attention as a fast extraction and cleanup method of pesticide residue analysis. Therefore, multi-residue analysis of 236 pesticides was tested with the QuEChERS method by concurrent use of PTV-GC/TOFMS (gas chromatography/ time-of-flight mass spectrometry with programmable temperature vaporizer). PTV condition was optimized and when the method was applied to apples, pesticide recovery rates (spiked at 400 ng/g) ranged from 80% to 120%, and RSD values were under 10% for most compounds. The results showed that the QuEChERS sample preparation and PTV-GC/TOFMS analysis can be applied to multi-residue analysis of pesticides in fruits and vegetables.

• Proceedings of the KSRS Conference
• /
• 2004.10a
• /
• pp.590-593
• /
• 2004

Extraction of ocean surface current velocity offers important physical oceanographic parameters especially on understanding ocean environment. Although Remote Sensing techniques were highly developed, the investigation of ocean surface current using Synthetic Aperture Radar (SAR) is not an easy task. This paper presents the results of ocean surface current observation using Doppler Centroid of ERS-1 SAR data obtained off the coast of Korea peninsula. We employed the concept, in which Doppler frequency shift and the ocean surface current are closely related, to evaluate ocean surface current. Moving targets cause Doppler frequency shift of the back scattered radar waves of SAR, thus the line-of-sight velocity component of the scatters can be evaluated. The Doppler frequency shift can be measured by estimating the difference between Doppler Centroid of raw SAR data and reference Doppler Centroid. Theoretically, the Doppler Centroid is zero; however, squinted antenna which is affected by several physical factors causes Doppler Centroid to be nonzero. The reference Doppler Centroid can be obtained from measurements of sensor trajectory, attitude and Earth model. The estimated Doppler Centroid was compensated by considering the accurate attitude estimation of ERS-1 SAR. We could verify the correspondence between the estimated ocean surface current and observed in-situ data in the error bound.

• Proceedings of the KSRS Conference
• /
• 2002.10a
• /
• pp.633-633
• /
• 2002

We investigated the distributions of sea ice using various microwave remote sensing techniques in the part of Drake passage, Antarctica, between the area 45-75$^{\circ}$W and 55-66$^{\circ}$S. We used Topex/Poseidon(T/P) radar altimeter, ERS-1 altimeter, ERS-2 scatterometer, Nimbus-7 Scanning Multichannel Microwave Radiometer (SMMR), and DMSP Special Sensor Microwave/Imager(SSM/I) data. The sea ice distributions were estimated between May and Jun., 1995 and Oct. and Nov., 1998. The two altimeter measurements (T/P and ERS-1) showed good coherence with the results from the radiometer data in the given period when the ice concentration of 20% and greater was selected. The scatterometer data also showed good correlation with altimetry-implied sea ice surface. The maximum and minimum values of sea ice distribution were appeared in Aug. and Feb., respectively. In general, the sea ice distributions estimated from radar altimeter, radioneter, and scatterometer are well correlated.

• Korean Journal of Remote Sensing
• /
• v.10 no.2
• /
• pp.49-62
• /
• 1994

Look direction bias in a single look SAR image can often be misinterpreted in the geological application of radar data. This paper investigates digital processing techniques for SAR image data integration and compensation of the SAR data look direction bias. The two important approaches for reducing look direction bias and integration of multiple SAR data sets are (1) principal component analysis (PCA), and (2) wavelet transform(WT) integration techniques. These two methods were investigated and tested with the ERS-1 (VV-polarization) and CCRS*s airborne (HH-polarization) C-SAR image data sets recorded over the Sudbury test site, Canada. The PCA technique has been very effective for integration of more than two layers of digital image data. When there only two sets of SAR data are available, the PCA thchnique requires at least one more set of auxiliary data for proper rendition of the fine surface features. The WT processing approach of SAR data integration utilizes the property which decomposes images into approximated image ( low frequencies) characterizing the spatially large and relatively distinct structures, and detailed image (high frequencies) in which the information on detailed fine structures are preserved. The test results with the ERS-1and CCRS*s C-SAR data indicate that the new WT approach is more efficient and robust in enhancibng the fine details of the multiple SAR images than the PCA approach.