• Title/Summary/Keyword: ER81

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ER81-shRNA Inhibits Growth of Triple-negative Human Breast Cancer Cell Line MDA-MB-231 In Vivo and in Vitro

  • Chen, Yue;Zou, Hong;Yang, Li-Ying;Li, Yuan;Wang, Li;Hao, Yan;Yang, Ju-Lun
    • Asian Pacific Journal of Cancer Prevention
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    • 제13권5호
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    • pp.2385-2392
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    • 2012
  • The lack of effective treatment targets for triple-negative breast cancers make them unfitted for endocrine or HER2 targeted therapy, and their prognosis is poor. Transcription factor ER81, a downstream gene of the HER2, is highly expressed in breast cancer lines, breast atypical hyperplasia and primary breast cancers including triple-negative examples. However, whether and how ER81 affects breast cancer carcinogenesis have remained elusive. We here assessed influence on a triple-negative cell line. ER81-shRNA was employed to silence ER81 expression in the MDA-MB-231 cell line, and MTT, colony-forming assays, and flow cytometry were used to detect cell proliferation, colony-forming capability, cell cycle distribution, and cell apoptosis in vitro. MDA-MB-231 cells stably transfected with ER81-shRNA were inoculated into nude mice, and growth inhibition of the cells was observed in vivo. We found that ER81 mRNA and protein expression in MDA-MB-231 cells was noticeably reduced by ER81-shRNA, and that cell proliferation and clonality were decreased significantly. ER81-shRNA further increased cell apoptosis and the residence time in $G_0/G_1$ phase, while delaying tumor-formation and growth rate in nude mice. It is concluded that ER81 may play an important role in the progression of breast cancer and may be a potentially valuable target for therapy, especially for triple negative breast cancer.

회전식 점도측정기를 이용한 ER 및 MR유체의 특성 비교 (The Comparison of Properties of ER and MR Fluids Using a Rotational Viscometer)

  • 이영록;전도영
    • 유변학
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    • 제11권2호
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    • pp.73-81
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    • 1999
  • 회전식 점도 측정기를 이용하여 상용화된 전기유변(electrorheological, ER)유체와 자기유변(magnetorheological, MR)유체의 특성을 비교하여 이를 이용한 장치 설계시 두 유체 중에 유리한 것을 선택하는데 도움이 되고자 하는 것이 본 논문의 의도이며, 이러한 유체의 특성에서 전단변형률의 변화에 따른 전단응력의 변화, 온도변화에 따른 전단응력의 변화, 작용하는 장의 세기에 따른 전단응력의 변화 등을 비교하였다. 온도의 변화에 따른 유체의 특성 변화는 MR유체가 ER유체보다 적게 받는 경향이 있었다. 두 유체의 응용 예로서 제작된 댐퍼의 경우에 유압 시험기를 이용하여 인장과 압축될 때 장의 On/Off에 따른 시간지연과 감쇄력을 비교하였다.

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MICROSTRUCTURE AND HIGH TEMPERATURE MECHANICAL PROPERTIES OF SAPPHIRE/R-Al-O (R=Y,Gd,Er,Ho,Dy) EUTECTIC FIRES GROWN BY MICRO PULLING-DOWN METHOD

  • Hasegawa, K.;Yoshikawa, A.;Durbin, S.;Epelbaum, B.;Fjkuda, T.;Waku, Y.
    • 한국결정성장학회:학술대회논문집
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    • 한국결정성장학회 1999년도 PROCEEDINGS OF 99 INTERNATIONAL CONFERENCE OF THE KACG AND 6TH KOREA·JAPAN EMG SYMPOSIUM (ELECTRONIC MATERIALS GROWTH SYMPOSIUM), HANYANG UNIVERSITY, SEOUL, 06월 09일 JUNE 1999
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    • pp.403-418
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    • 1999
  • Fiber growth of Al2O3/R-Al-O(R=Y, Gd, Dy, Ho, Er) eutectic by the micro-pulling down methods is described. The thermal stability and strength at elevated temperature of each material is evaluated in relation to the microstructure. PACS: 81.05 Mh, 81.10 Fq, 81.30-t.

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Post-diagnosis Soy Food Intake and Breast Cancer Survival: A Meta-analysis of Cohort Studies

  • Chi, Feng;Wu, Rong;Zeng, Yue-Can;Xing, Rui;Liu, Yang;Xu, Zhao-Guo
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권4호
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    • pp.2407-2412
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    • 2013
  • Background and Objectives: Data on associations between soy food intake after cancer diagnosis with breast cancer survival are conflicting, so we conducted this meta-analysis for more accurate evaluation. Methods: Comprehensive searches were conducted to find cohort studies of the relationship between soy food intake after cancer diagnosis and breast cancer survival. Data were analyzed with comprehensive meta-analysis software. Results: Five cohort studies (11,206 patients) were included. Pooling all comparisons, soy food intake after diagnosis was associated with reduced mortality (HR 0.85, 95%CI 0.77 0.93) and recurrence (HR 0.79, 95%CI 0.72 0.87). Pooling the comparisons of highest vs. lowest dose, soy food intake after diagnosis was again associated with reduced mortality (HR 0.84, 95%CI 0.71 0.99) and recurrence (HR 0.74, 95%CI 0.64 0.85). Subgroup analysis of ER status showed that soy food intake was associated with reduced mortality in both ER negative (highest vs. lowest: HR 0.75, 95%CI 0.64 0.88) and ER positive patients (highest vs. lowest: HR 0.72, 95%CI 0.61 0.84), and both premenopausal (highest vs. lowest: HR 0.78, 95%CI 0.69 0.88) and postmenopausal patients (highest vs. lowest: HR 0.81, 95%CI 0.73 0.91). In additioin, soy food intake was associated with reduced recurrence in ER negative (highest vs. lowest: HR 0.64, 95%CI 0.44 0.94) and ER+/PR+ (highest vs. lowest: HR 0.65, 95%CI 0.49 0.86), and postmenopausal patients (highest vs. lowest: HR 0.67, 95%CI 0.56 0.80). Conclusion: Our meta-analysis showed that soy food intake might be associated with better survival, especially for ER negative, ER+/PR+, and postmenopausal patients.

충적과정 중 미국삼 종자의 생리 및 생화학적 지표에 관한 동태학적 연구 I. 배아율, 건조중량비 및 호흡강도 (Dynamic Studies on Physiology and Biochemistry in American Seng Seed During Stratification - Part I. Embryo Ratio, Dry Weight Ratio and respiration Rate)

  • Huang, Yao-Ge;Li, Xiang-Gao;Yang, Ji-Xiang;Kuang, Ya-Lan;Yan, Jie-Kun;Cui, Shu-Yu;Liu, Ren-Song;Kim, Hack-Seang
    • Journal of Ginseng Research
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    • 제20권3호
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    • pp.325-330
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    • 1996
  • Dynamic parameters of physiology including embryo ratio (ER), embryo length to endosperm length, dry weight ratio between embryo and endosperm (DWR) and respiration rate (RR) in American send (Panax quinquefolium L.) seed were investigated. According to the changes of ER during seed stratification, the duration of embryo afterripening could be divided into three stages as \circled1 embryo slow growth stage (ESGS), ER increased from 7.31% to 20.48% (0.16% day-1): \circled2 embryo rapid growth stage (ERGS), ER increased to 80.98% (0.61% day-1) (75G5+ ERGS=morphological afterripening stage (MP,5)) and \circled3pysiological afterripening stage (PAS), ER Increased to 88.50% (0.094 day-1) only. DWR Increased slowly from 0.20% to 2.76clp (0.016% day-1) in MAS and rapidly to 8.81% (0.061% day-1) in PAS. The RR correlated significantly with ER as well (r=0.8934 > rot, 0.6610). The steep increment of both DWR and RR in PAS indicated that the PAS was not a static stage although the ER was not changed too much. All of these may provide some information for understanding the dormancy mechanisms of American sting seed.

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Model order reduction for Campbell diagram analysis of shaft-disc-blade system in 3D finite elements

  • Phuor, Ty;Yoon, GilHo
    • Structural Engineering and Mechanics
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    • 제81권4호
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    • pp.411-428
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    • 2022
  • This paper presents the Campbell diagram analysis of the rotordynamic system using the full order model (FOM) and the reduced order model (ROM) techniques to determine the critical speeds, identify the stability and reduce the computational time. Due to the spin-speed-dependent matrices (e.g., centrifugal stiffening matrix), several model order reduction (MOR) techniques may be considered, such as the modal superposition (MS) method and the Krylov subspace-based MOR techniques (e.g., Ritz vector (RV), quasi-static Ritz vector (QSRV), multifrequency quasi-static Ritz vector (MQSRV), multifrequency/ multi-spin-speed quasi-static Ritz vector (MMQSRV) and the combined Ritz vector & modal superposition (RV+MS) methods). The proposed MMQSRV method in this study is extended from the MQSRV method by incorporating the rotational-speed-dependent stiffness matrices into the Krylov subspace during the MOR process. Thus, the objective of this note is to respond to the question of whether to use the MS method or the Krylov subspace-based MOR technique in establishing the Campbell diagram of the shaft-disc-blade assembly systems in three-dimensional (3D) finite element analysis (FEA). The Campbell diagrams produced by the FOM and various MOR methods are presented and discussed thoroughly by computing the norm of relative errors (ER). It is found that the RV and the MS methods are dominant at low and high rotating speeds, respectively. More precisely, as the spinning velocity becomes large, the calculated ER produced by the RV method is significantly increased; in contrast, the ER produced by the MS method is smaller and more consistent. From a computational point of view, the MORs have substantially reduced the time computing considerably compared to the FOM. Additionally, the verification of the 3D FE rotordynamic model is also provided and found to be in close agreement with the existing solutions.

METABOLIZABLE ENERGY REQUIREMENTS FOR MAINTENANCE AND GROWTH OF SUCKLING CALVES GIVEN MILK REPLACER

  • Sekine, J.;Oura, R.;Morita, Z.;Morooka, T.;Asahida, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • 제1권2호
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    • pp.61-63
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    • 1988
  • A total of 38 energy balance trials were done for calves fed a liquid milk replacer, calf starter and second cut mixed hay. Milk replacer supplied 81% of the total dietary energy. Live weight of calves averaged 54.1 (S.D 6.2) kg and daily gain 0.37 (${\pm}0.23$)kg. The metabolizability of gross energy averaged 0.822. A regression was calculated relating energy retention (ER, $kJ/kg^{0.75}$) to the intake of metabolizable energy (IME, $kJ/kg^{0.75}$): ER = 0.72 (${\pm}0.12$) IME - 330, r = 0.702, P < 0.01, $S.E.{\pm}18.0$. Metabolizable energy for maintenance (MEm) was calculated to be $458kJ/kg^{0.75}$ when ER = 0. The amount of IME over MEm for an individual animal (Meg, $kJ/kg^{0.75}$) was regressed on averaged daily gain (ADG, kg): Meg = 413 (${\pm}91$) ADG + 0.2, r = 0.650, P < 0.01, $S.E.{\pm}21$. The amount of ME requirement for suckling calves was estimated using values obtained above.

Growth of abalone (Haliotis discus hannai) in cages using epibiont control measures

  • Han, Jido;Jeon, Mi Ae;Kim, Da Woon;Park, Hon;Kim, Byong Hak;Lee, Deok Chan
    • Fisheries and Aquatic Sciences
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    • 제24권12호
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    • pp.400-405
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    • 2021
  • In this study, the relationship between the growth of abalone and the presence of epibionts was investigated in abalone cultured in Goheung, Jeollanam-do, where there are severe problems high water temperatures and attachment. The experiment was conducted for eight months (May-December 2020), and 40 abalone were collected every month. Water temperature was at its highest at a range of 13.5℃-26.6℃ and dissolved oxygen levels were at their lowest at a range of 4.0-10.2 ㎍/L in August. The shell height (mm) of abalone grew to 117.7% (81.8 ± 1.9 mm) in cultures where epibionts were removed (ER) and 111% (77.4 ± 3.3 mm) where they were not (non-epibionts, NER). Their total weight (TW) and body weight increased significantly and steadily with ER, whereas the TW increased sharply after August with NER. In the condition index, no significant difference was observed between ER and NER. The monthly proportion of epibionts increased significantly in July, accounting for the value of 69.9% reached in December.

전기점성 유체를 이용한 동력전달 장치에 관한 연구 (A Study on Torque Transmission Using Electro-Rheological Fluid)

  • 주동우;이은준;박명관
    • 한국정밀공학회지
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    • 제15권10호
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    • pp.81-87
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    • 1998
  • This paper provides an investigation of torque transmission system using ERF (Electro-Rheological Fluid). The torque transmission system using ERF is a new conception device because an apparent viscosity of ERF can be changed by applying an electric field, We use the coaxial cylinder type in which the ERF fills the annular space between a pair of coaxial cylindrical electrodes and experiment results show that the measured torque was increased with the increase of the electric field. These are analyzed to provide guidelines to assist in the development of practical ER devices.

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