• Title/Summary/Keyword: EMT-I

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Mitofusin-2 enhances cervical cancer progression through Wnt/β-catenin signaling

  • Sung Yong Ahn
    • BMB Reports
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    • v.57 no.4
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    • pp.194-199
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    • 2024
  • Overexpression of mitofusin-2 (MFN2), a mitochondrial fusion protein, is frequently associated with poor prognosis in cervical cancer patients. Here, I aimed to investigate the involvement of MFN2 in cervical cancer progression and determine the effect of MFN2 on prognosis in cervical cancer patients. After generating MFN2-knockdown SiHa cells derived from squamous cell carcinoma, I investigated the effect of MFN2 on SiHa cell proliferation using the Cell Counting Kit-8 assay and determined the mRNA levels of proliferation markers. Colony-forming ability and tumorigenesis were evaluated using a colony-formation assay and tumor xenograft mouse models. The migratory and invasive abilities associated with MFN2 were measured using wound-healing and invasion assays. Wnt/β-catenin-mediated epithelial-mesenchymal transition (EMT) markers related to MFN2 were assessed through quantitative RT-PCR. MFN2-knockdown SiHa cells exhibited reduced proliferation, colony formation, migration, invasion, and tumor formation in vivo. The motility of SiHa cells with MFN2 knockdown was reduced through Wnt/β-catenin-mediated EMT inhibition. MFN2 promoted cancer progression and tumorigenesis in SiHa cells. Overall, MFN2 could serve as a therapeutic target and a novel biomarker for cervical cancer.

Luteolin Induces Apoptosis via Mitochondrial Pathway and Inhibits Invasion and Migration of Oral Squamous Cell Carcinoma by Suppressing Epithelial-Mesenchymal Transition Induced Transcription Factors

  • Park, Bong-Soo;Kil, Jong-Jin;Kang, Hae-Mi;Yu, Su-Bin;Park, Dan-Bi;Park, Jin-A;Kim, In-Ryoung
    • International Journal of Oral Biology
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    • v.43 no.2
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    • pp.69-76
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    • 2018
  • Oral squamous cell carcinoma (OSCC) is the most common type of oral malignancy. Numerous therapies have been proposed for its cure. Research is continually being conducted to develop new forms of treatment as current therapies are associated with numerous side-effects. Luteolin, a common dietary flavonoid, has been demonstrated to possess strong anti-cancer activity against various human cancer cell lines. Nevertheless, research into luteolin-based anticancer activity against oral cancer remains scarce. Thus, the objective of this study was to assess the effect of luteolin as an anti-cancer agent. After treatment with luteolin, Ca9-22 and CAL-27 oral cancer cells showed condensed nuclei and enhanced apoptotic rate with evidence of mitochondria-mediated apoptosis. Epithelialmesenchymal transition (EMT) is closely related to tumor migration and invasion. Luteolin suppressed cancer cell invasion and migration in the current study. Elevated expression of E-cadherin, an adherens junction protein, was evident in both cell lines after luteolin treatment. Luteolin also significantly inhibited transcription factors (i.e., N-cadherin, Slug, Snail, Twist, and ZEB-1) that regulated expression of tumor suppressors such as E-cadherin based on Western blot analysis and quantitative PCR. Thus, luteolin could induce mitochondrial apoptosis and inhibit cancer cell invasion and migration by suppressing EMT-induced transcription factors.

The effects of psychological factors on elderly of depression scale and Electroephalography

  • Kang, Byeong-Woo;Jung, Mi-Hyun;Cho, Byung-Jun
    • Journal of the Korea Society of Computer and Information
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    • v.21 no.12
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    • pp.157-163
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    • 2016
  • Purpose : This study purposed on the understanding of psychology in the elderly women using depression scale and electroephalography. The subjects were thirty elderly women in geriatric care hospital. All participants were elderly women over 65 years old. The subjects signed up with informed consent and they were divided into a normal elderly group, an exercise elderly group and a hospital elderly group. They were randomly assigned with 10 persons. Methods : The study instruments were Geriatric Depression Scale(GDS) and Poly-G-I. Brain wave activity was measured by 'power spectrum analysis' of TeleScan program. Statistic analysis consisted of average, standard deviation, One-way ANOVA and post-hoc Tukey using SPSS 21.0 version. The significance was set at .05. Results : Depression scale results showed that the exercise elderly group were $10.60{\pm}5.36$ points which was the lowest among the three groups. The Normal elderly group was $16.20{\pm}5.59$, and the hospitalized elderly group was $16.70{\pm}6.76$ points. There was no significant difference between the normal elderly group, exercise elderly group and hospitalized elderly group. The hospitalized group showed statistical difference in relative theta power in the area of Fp1, F3, F4, T3, P3 as compared to the normal elderly group and the exercise elderly groups. There was no significant difference between relative beta power and relative gamma power in three groups. Conclusion : Geriatric depressed scale showed no significant difference in each group. This is indicated in the mental problems associated with depression. This indicates a higher level of depression in the hospitalized elderly women, more than in the normal elderly women and exercise elderly women groups.

A Study on the survey of ethical dilemmas of fire fighters and paramedics (응급 및 재난현장에서 소방관의 윤리의식에 대한 조사)

  • Kim, Jee-Hee;Jeong, Jung-Sik
    • 한국방재학회:학술대회논문집
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    • 2008.02a
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    • pp.377-379
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    • 2008
  • This study was focused on the ethical dilemmas of fire fighters in Korea. There was no research on ethical dilemmas and experience of fire fighters and paramedics(emergency medical technician, EMT) in Korea. So I am going to survey this ethical problems through questionnaires and propose the basic data for the legal and ethical solutions.

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Evaluation of the Triage by Emergency Medical Technicians by Using Trauma Score for Occupant Injuries Caused by Motor Vehicle Collisions (자동차 탑승자 사고에서 외상계수를 이용한 구급대원의 중증도 분류 평가)

  • Kim, Sang Chul;Kim, Byung Woo;Tak, Yang Ju;Lee, Sang Hee
    • Journal of Trauma and Injury
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    • v.26 no.3
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    • pp.89-98
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    • 2013
  • Purpose: The assessment of trauma patients in the prehospital setting is difficult, but appropriate field triage is critical to the prognosis of trauma patients. We sought to evaluate the triage given by the emergency medical technicians (EMTs) using the trauma score to patients injured in motor vehicle collisions (MVCs). Methods: From June 2012 to July 2012, questionnaires were distributed to EMTs, who had transported injured patients to the study hospital. Scene records, photos of the damaged vehicle, and ambulance run sheets were used to provide physiologic, physical, and mechanistic information about the MVC. To evaluate the appropriateness of the injury assessment by EMTs, we compared their impressions with the hospital's final diagnosis within a 3 level triage system comprising both the maximum abbreviated injury scale (MAIS) and the injury severity score (ISS). Kappa (k) was calculated to evaluate the agreement between the triage by EMTs and the triage based on hospital's final diagnosis. Results: A total of 91 patients were analyzed by 31 EMTs. The percentage of males was 57.1%, the mean age was 44.5, and the mean MAIS and ISS were 2.7 and 16.6 respectively. While EMTs correctly diagnosed patient injuries to the extremities in 35.7%, and to the neck in 32.1%, pelvic injuries were missed in 80.0%. The agreement between the triage by the EMTs and the triage based on the hospital's final diagnosis was 62.6%(k=0.366) by the MAIS and 50.5%(k=0.234) by the ISS. The kappa value was higher in EMT-I than in EMT-II. Conclusion: In MVC, the assessment of injured patients by EMT-I was more appropriate, and the 3-level triage method based on the MAIS could contribute to a more accurate triage. Prospective studies to search for appropriate methods of field triage are required for programming practical education for EMTs.

Assessing the forensic knowledge of 119 emergency medical technicians (119 구급대원의 법의학 지식에 대한 연구)

  • Lee, Hee-Young;Moon, Jun-Dong
    • The Korean Journal of Emergency Medical Services
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    • v.23 no.2
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    • pp.75-86
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    • 2019
  • Purpose: It is crucial that 119 emergency medical technician (EMT) have adequate knowledge regarding forensic science. This study aimed to assess the forensic knowledge of EMTs in the Republic of Korea. Methods: This study was conducted on EMTs of five fire stations in D metropolitan city between August 6 and August 13, 2018. A questionnaire consisting of items on crime-related incident recognition (i.e., mechanical asphyxiation, trauma, sex crimes, and child abuse), forensic evidence management (i.e., evidence collection, preservation, and recording) was administered to the participants. Finally, 119 questionnaires were analyzed. Results: The ratio of correct answers for crime-related incidents recognition was 55.82%, which was lower than that for forensic evidence management(84.5%). In particular, the scores for the type of neck compression(16.5%) and wound assessment(44.0%) were low. The percentage of correct answers for forensic evidence management was 78.4% for evidence collection, 84.4% for evidence preservation, and 90.6% for evidence recording. Previous forensic education experience was not a significant variable. Conclusion: Specific and practical forensic science education on perception of crime-related incident, including asphyxia and wound identification, and forensic evidence collection is needed.

Direction of Emergency Rescue Education Based on the Experience of New 119 Paramedics for National Health Promotion (국민건강증진을 위한 응급구조학 교육의 나아갈 방향 -신임 119구급대원의 출동경험을 바탕으로-)

  • Kim, Jung-Sun
    • Journal of Korea Entertainment Industry Association
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    • v.15 no.1
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    • pp.207-220
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    • 2021
  • The purpose of the study is to investigate the application and utility of emergency rescue education and derive limitations, improvements and development directions of university education based on the field experience of 119 emergency medical technician(EMT)s. The research subjects were six new 119 emergency medical technician(EMT)s within three years of starting their first-aid service in the field. After conducting in-depth narrative interviews, the analysis was performed using Colaizzi method. The 82 formulated meanings were derived from significant statements. From formulated meanings, 23 themes, 4 theme clusters, 2 categories were identified. The four theme clusters were 'The effectiveness of university education', 'The limitations of university education', 'The direction of improvement in educational methodology' and 'The direction of improvement in educational contents. University education has been helpful overall, but limitations are observed at the same time, suggesting that it should be developed through the improvement of educational methodologies (i.e. problem-based learning, field case review, education through role-playing, simulation education, strengthening skill ect.) and educational content (i.e. training tailored to the field, education focused on trauma or cardiac arrest, expansion of triage education in disaster management, reinforcement of education on-site safety, education on special patients, diverse guidance and faculty for different perspectives).

A Study on Affecting Stress Factors of EMT Students (일부 대학의 응급구조과 학생의 스트레스에 관한 고찰)

  • Jo, Jeanman;Kim, Hak-Soo;Kim, jin-Hue
    • The Korean Journal of Emergency Medical Services
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    • v.4 no.1
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    • pp.73-81
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    • 2000
  • This is a study to evaluate the effects of the stress and figure out the cause of them, I surveyed 400 students (male 257 and female 143) of health sciences colleges from 15th October to 23th October 1999. The major instruments of this study were Korean Style Self-Analysis Stress Opinionnaire. This Questionnaire contains 5 items which measure stress and health opinions or attitudes. The data were analyzed by the path analysis - with SPSS and AMOS package program. The result are as follows : 1. In the comparison of stress by sex, a potential group is highest for both of male and female (male 35.7%, female 64.3%). In high risk group, male is 10.6% and female 13.0%. In potential risk group, male is 4.1% and female 7.6%. 2. It have suggested that students' stress factors much affected with physical health and psychological health in school life(Y(Stress) = $0.813{\times}1$(Health) - $0.381{\times}2$(Mind Health) + $0.118{\times}3$(School Social) + $0.100{\times}4$(Confidence) - $0.061{\times}5$(Happiness Social)+E).

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Comparison of knowledge level of triage in 119 EMTs (119 구급대원의 중증도 분류 지식 정도 및 교육 전·후 비교)

  • Lee, Hyo-Ju;Cho, Keun-Ja
    • The Korean Journal of Emergency Medical Services
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    • v.18 no.1
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    • pp.43-54
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    • 2014
  • Purpose : The purpose of this study was to provide appropriate direction for triage education by investigating the knowledge level of triage in 119 EMTs before and after the triage education. Methods : The questionnaire was filled out by newly assigned 33 EMTs in the fire service academy in I metropolitan city from November 1, 2013. The data were analyzed using SPSS WIN 21.0 program. Results : The lowest knowledge level by 119 triage was the potential emergency related question, but that by case-based triage was the semi-emergency related questions. The knowledge score by case-based triage before education was 51.14 points. This was lower than knowledge score by 119 triage, that is, 75.70 points. After education, the knowledge level by 119 triage was significantly improved(p =.000). However, there was no significant difference in the knowledge level by case-based triage(p =.236). Conclusion : It is necessary to provide systematic and periodic education and training for 119 EMTs toward triage to improve triage accuracy and efficient circulation of the emergency medical service system. Especially, it is very important to provide case-based triage education for field application.

Genistein alleviates pulmonary fibrosis by inactivating lung fibroblasts

  • Seung-hyun Kwon;Hyunju Chung;Jung-Woo Seo;Hak Su Kim
    • BMB Reports
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    • v.57 no.3
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    • pp.143-148
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    • 2024
  • Pulmonary fibrosis is a serious lung disease that occurs predominantly in men. Genistein is an important natural soybean-derived phytoestrogen that affects various biological functions, such as cell migration and fibrosis. However, the antifibrotic effects of genistein on pulmonary fibrosis are largely unknown. The antifibrotic effects of genistein were evaluated using in vitro and in vivo models of lung fibrosis. Proteomic data were analyzed using nano-LC-ESI-MS/MS. Genistein significantly reduced transforming growth factor (TGF)-β1-induced expression of collagen type I and α-smooth muscle actin (SMA) in MRC-5 cells and primary fibroblasts from patients with idiopathic pulmonary fibrosis (IPF). Genistein also reduced TGF-β1-induced expression of p-Smad2/3 and p-p38 MAPK in fibroblast models. Comprehensive protein analysis confirmed that genistein exerted an anti-fibrotic effect by regulating various molecular mechanisms, such as unfolded protein response, epithelial mesenchymal transition (EMT), mammalian target of rapamycin complex 1 (mTORC1) signaling, cell death, and several metabolic pathways. Genistein was also found to decrease hydroxyproline levels in the lungs of BLM-treated mice. Genistein exerted an anti-fibrotic effect by preventing fibroblast activation, suggesting that genistein could be developed as a pharmacological agent for the prevention and treatment of pulmonary fibrosis.