• ALGAE
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• v.34 no.1
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• pp.57-70
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• 2019

DNA metabarcoding is currently used for large-scale taxonomic identification to understand the community composition in various marine ecosystems. However, before being widely used in this emerging field, this experimental and analytic approach still has several technical challenges to overcome, such as polymerase chain reaction (PCR) bias, and lack of well-established metabarcoding markers, a task which is difficult but not impossible to achieve. In this study, we present an adapted PCR-free small-organelles enriched metagenomics (SoEM) method for marine biodiversity assessment. To avoid PCR bias and random artefacts, we extracted target DNA sequences without PCR amplification from marine environmental samples enriched with small organelles including mitochondria and plastids because their genome sequences provide a valuable source of molecular markers for phylogenetic analysis. To experimentally enrich small organelles, we performed subcellular fractionation using modified differential centrifugation for marine environmental DNA samples. To validate our SoEM method, two marine environmental samples from the coastal waters were tested the taxonomic capturing capacity against that of traditional DNA metabarcoding method. Results showed that, regardless of taxonomic levels, at least 3-fold greater numbers of taxa were identified in our SoEM method, compared to those identified by the conventional multi-locus DNA metabarcoding method. The SoEM method is thus effective and accurate for identifying taxonomic diversity and presents a useful alternative approach for evaluating biodiversity in the marine environment.

• Korean Journal of Weed Science
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• v.30 no.3
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• pp.272-281
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• 2010

The objective of this research was to find out the weed management techniques in environment-friendly rice paddy fields through the study on herbicidal effects and problems of various organic materials. This experiment was conducted under different conditions of weed species and weed densities in environment-friendly rice paddy fields. There was no difference in weedy efficacy on golden apple snail (GAS), paper mulching (PM), and machine weeding (MW) between low and high weed densities. However, the effect of weed control in rice bran (RB) and effective microorganism (EM) + molasses was higher in high weed density than in low weed density. In general, the effect of weed control as affected by various organic materials was in the order of GAS (97-100%) > PM (93-98%) > RB (15-80%) > EM (7-31%). GAS provided excellent control of all weed species tested except for Persicaria hydropiper. PM gave acceptable control of the weed species except for Echinochloa crus-galli, Ludwigia prostrata, and Eleocharis kuroguwai. However, MW gave fair control (70% biomass reduction) of all weed species tested. BR followed by MW or EM followed by MW treatments had similar effect on weed control compared to each treatment alone. However, BR followed by GAS or EM followed by GAS provided 100% control of weed species tested. The level of rice foliar injury caused by various organic materials was in the order of GAS and MW (10-20%) > RB (10-15%) > PM and EM (5-7%). Typical symptoms of organic materials are wilting, inhibition of growth, missing hill, and reduction of tiller. Cost for weed control of GAS, RB, EM, and PM were 2.1, 3.1, 2.3, and 13.2 times higher than that of the herbicide. These data indicate that GAS was the best method for weed management in environmentfriendly rice paddy fields. Further study is required to elucidate the mechanisms underlying the rice injury as affected by GAS.

• Journal of Electrical Engineering and Technology
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• v.6 no.4
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• pp.513-518
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• 2011

In this paper, a unified design environment is developed for the optimization of electric machines based on continuum sensitivity. For electromagnetic (EM) system analysis, COMSOL scripting environment is used. Optimization module is developed by MATLAB programming, which can be combined with COMSOL script commands. The modules are combined into one MATLAB project, and iteration process necessary for the optimization of EM system can be performed efficiently. During the design process, visual feedback of the current design status is given to the designer. In addition, the B-Spline parametrization of the nodal points is implemented to obtain smooth boundary of the device. The developed software is applied to the problem of finding uniform flux density distribution at the air gap of an electromagnet to verify its feasibility and effectiveness.

• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.587-601
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• 2013

During the last few decades, it has become evident that the compatibility of the yeast biochemical environment with the ability to process and translate the RNA transcript, along with its capacity to modify a translated protein, are relevant requirements for selecting this host cell for protein expression in several pharmaceutical and clinical applications. In particular, Pichia pastoris is used as an industrial host for recombinant protein and metabolite production, showing a powerful capacity to meet required biomolecular target production levels in high-throughput assays for functional genomics and drug screening. In addition, there is a great advantage to using P. pastoris for protein secretion, even at high molecular weights, since the recovery and purification steps are simplified owing to relatively low levels of endogenous proteins in the extracellular medium. Clearly, no single microexpression system can provide all of the desired properties for human protein production. Moreover, chemical and physical bioprocess parameters, including culture medium formulation, temperature, pH, agitation, aeration rates, induction, and feeding strategies, can highly influence product yield and quality. In order to benefit from the currently available wide range of biosynthesis strategies using P. pastoris, this mini review focuses on the developments and technological fermentation achievements, providing both a comparative and an overall integration analysis. The main aim is to highlight the relevance and versatility of the P. pastoris biosystem to the design of more cost-effective microfactories to meet the increasing demands for recombinant membrane proteins and clinical antibodies for several therapeutic applications.

• Geophysics and Geophysical Exploration
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• v.13 no.2
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• pp.175-180
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• 2010

The small loop electromagnetic (EM) method is a fast and convenient geophysical tool which can provide resistivity distribution of shallow subsurface. Especially, it can be a useful alternative of resistivity method in a very conductive environment such as a reclaimed saline land. We applied the multi-frequency small loop EM method for the site investigation of reclaimed saline land. We inverted the measured EM data using one dimensional (1D) inversion program and merged to obtain three dimensional (3D) resistivity distribution over the survey area. Finally, comparing he EM results with the drill log and measured soil resistivity sampled at 16 drill holes, we can define the site character such as thickness of landfill, salinity distribution, and etc.

• ETRI Journal
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• v.9 no.2
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• pp.96-105
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• 1987

The purpose of this paper is to report the implementation of new frame synchroniazation circuit for D3/PCM.When the D3/PCM signal(1.544Mb/s) is received, the performance of the new frame synchronizer is evaluated by the statistical method according to the parameters BER,es, em. For BER=$10^-2$, es=0 and em=2, the implemented hardware will render the initial search time, the maintenance time and there frame time equals to 2.87ms, 9.9hrs and2.69ms, respectively. Also the proposed frame synchronizer can be easily modified to the practical transmission channels environment and the new 1.544Mb/s signal format.

• Geophysics and Geophysical Exploration
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• v.20 no.4
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• pp.216-225
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• 2017

Recently, steel casing became an interesting issue when applying controlled-source electromagnetic (EM) method to various fields because effects of steel casing on EM responses are not negligible. This study employed an approach that approximates the steel casing as a series of electric dipole sources in order to develop the numerical algorithm for the efficient simulation of EM responses in the presence of steel casing. After verifying the validity of the developed algorithm, we analyze effects of steel casing on EM responses with the synthetic model simulating geothermal reservoir environment. The analysis showed that the effects of steel casing on EM responses are localized near the casing and increase as the transmitter becomes close to the casing. In addition, through the analysis on the EM responses by the injection of clean water, we confirm that the effects of casing are negligible when interpreting the after-injection data acquired using the transmitter located far enough from the casing. Considering the difference in EM responses between before and after injection in inversion, the effects of the casing can be neglected although after-injection data shows considerable difference due to the close distance between the transmitter and casing. To investigate this kind steel casing effect, the precise analysis on EM responses should be preceded. The algorithm introduced in this study will contribute to the reliable calculations of EM responses distorted by the conductive steel casing.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.24 no.12
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• pp.1158-1166
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• 2013

SAR calculation method following the Mobi-Kids study protocol is analyzed and evaluation method of cumulative RF dose from mobile phones which have been used by a subject of case and control groups is proposed. An SAR database is built by calculating SAR distributions in 4 head models at different ages for representative phone models with the same conducted power. To obtain SAR distribution in a subject's head for a specific commercial phone which had/have been used by him/her, an SAR correction factor using SAR compliance test results is determined. Cumulative dose is calculated by considering mobile phone characteristics and use pattern such as call time and laterality(right and left).

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.20 no.12
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• pp.1128-1138
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• 2010

One of the typical problems in the precise vibration is resonance characteristics at low frequency disturbance due to a heavy mass. An electro-magnetic(EM) air spring is a kind of vibration control unit and active isolator. The EM air spring in this study aims at removing the low frequency resonance for semiconductor manufacturing. The mechanical and electronic parts in the active isolator are designed to operate under a weight of 2.5 tons. The EM spring is floated using air pressure in a pneumatic elastic chamber and actuated by EM levitation force. The actuator consists of a EM coil and a permanent magnetic plate which are installed inside of the chamber. An air mount was constructed for the experiment with a stone surface plate, 4 active air springs, 4 gap sensors, a DSP controller, and a multi-channel power amp. A PD control method and operating logic was applied to the DSP. Simulation using 1/4 model was carried out and compared with the experiments. The time duration and maximum peak at resonance frequency can be reduced sharply by the proposed system. The results show that the active system can avoid the resonance caused by the natural frequency of the passive system.

• The Journal of the Korea institute of electronic communication sciences
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• v.12 no.3
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• pp.427-432
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• 2017

This study describes the design and implementation of SHF band Downconverter Digital Satellite Communication. The SHF band Downconverter unit consists of PLDRO and Frequency converter. In Frequency converter, microstrip BPF and LPF designed through the pre EM simulation are implemented to minimize the unwanted spurious in Frequency converter. Through the pre-simulation analysis of space environment, the possibility of and minimized about the malfunction of equipment and we designed a reliable SHF band Downconverter through simulation for a TID according to the vibration generated during the launch and space radiation environment, and compared pre-simulation of main performance results to test results about main performances of SHF band Downconverter after production.