• Title/Summary/Keyword: EHV

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The first virus isolation and partial characterization of equine herpesvirus-4 in a horse, South Korea

  • Choi, Eun-Jin;Lee, Hyun-Kyoung;Lee, Kyoung-Hyun;So, Byoung-Jae;Song, Jae-Young;Do, Jae-Chul;Yang, Seon-Joo;Lee, Hyun-Chul;Yang, Young-Jin
    • Korean Journal of Veterinary Service
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    • v.38 no.2
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    • pp.141-144
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    • 2015
  • An equine herpesvirus-4 (EHV-4) was isolated in nasal swabs collected in a horse showing respiratory clinical signs. Equine dermis cells inoculated with the sample were observed with characteristic viral cytopathic effects after 3 days of postinoculation and the infected cells exhibited bright intracelluar fluorescence by indirect immunofluorescence assay. At the nucleotide level, the partial glycoprotein B gene of the Korean EHV-4 isolate (K001) had 99.9% identity to 1942 strain (GenBank No. M26171). To author's knowledge, the report describes the first isolation and partial characterization of EHV-4 in Korea. The virus can be used for further study of EHV-4.

Epizootiologic evaluation on equine coital exanthema in Korea (국내 발생 말구진의 역학적 평가)

  • Yang, Jaehyuk;Lim, Yoon-Kyu
    • Korean Journal of Veterinary Research
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    • v.52 no.1
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    • pp.61-64
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    • 2012
  • This study evaluated the epizootiological characteristics of equine coital exanthema (ECE) in South Korea. A PCR test was used to determine the equine herpesvirus 3 (EHV-3) release period, excretion by suckling foals, morbidity rate, effect on fertility, and onset of breeding partner after treatment. The morbidity rate of ECE based on clinical symptoms was 8.3% (2/24) for stallions and 10.8% (45/416) for mares, and 29 of 45 (64.4%) animals were positive on the PCR test. Ten (22%) broodmares had symptoms before breeding, while 26 (58%) had symptoms after breeding. Nine (20%) mares had uncertain coverage periods and occurrence times. Suckling foals had no clinical findings and EHV-3 was not detected in their nostrils, although it was detected on teasers. No lesions were observed in the clitoral fossa on broodmares, although EHV-3 was detected by PCR. The period of EHV-3 emission was 22~23, 18~19, 6, and 58 days in stallions, broodmares, teasers, and mares with a mixed E. coli-like infection, respectively. ECE had no negative effects on the breeding capability of stallions and no symptoms were observed in broodmares after recovering from ECE.

A 3-D Steady-State Analysis of Thermal Behavior in EHV GIS Busbar

  • Lei, Jin;Zhong, Jian-ying;Wu, Shi-jin;Wang, Zhen;Guo, Yu-jing;Qin, Xin-yan
    • Journal of Electrical Engineering and Technology
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    • v.11 no.3
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    • pp.781-789
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    • 2016
  • Busbar has been used as electric conductor within extra high voltage (EHV) gas insulated switchgear (GIS), which makes EHV GIS higher security, smaller size and lower cost. However, the main fault of GIS is overheating of busbar connection parts, circuit breaker and isolating switch contact parts, which has been already restricting development of GIS to a large extent. In this study, a coupled magneto-flow-thermal analysis is used to investigate the thermal properties of GIS busbar in steady-state. A three-dimensional (3-D) finite element model (FEM) is built to calculate multiphysics fields including electromagnetic field, flow field and thermal field in steady-state. The influences of current on the magnetic flux density, flow velocity and heat distribution has been investigated. Temperature differences of inner wall and outer wall are investigated for busbar tank and conducting rod. Considering the end effect in the busbar, temperature rise difference is compared between end sections and the middle section. In order to obtain better heat dissipation effect, diameters of conductor and tank are optimized based on temperature rise simulation results. Temperature rise tests have been done to validate the 3-D simulation model, which is observed a good correlation with the simulation results. This study provides technical support for optimized structure of the EHV GIS busbar.

Characterization of the molecular and biological properties between the equine herpesvirus type 1 immediate-early protein and the general transcription factor human TFIIB

  • Jang Hyung-Kwan
    • Korean Journal of Veterinary Service
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    • v.27 no.4
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    • pp.355-369
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    • 2004
  • The equine herpesvirus type 1 (EHV-1) immediate-early (IE) protein is a potent transactivator responsible for the activation of both early and late genes during the course of infection and is comprised of discrete functional domains that mediate its many functions. Interaction between trans activators such as the IE protein and various components of the RNA polymerase II transcription initiation machinery has been demonstrated to be critical for transactivation. In the present report, it is addressed the hypothesis that the IE protein interacts with various components of transcription machinery to mediate transactivation of target viral genes. In these studies, it is demonstrated that in vitro transcribed and translated IE protein interacts with TFIIB-agarose conjugate but not with TFIID-agarose conjugate. Additional immunoprecipitation studies using nuclear extracts derived from EHV-1 infected RK-13 cells confirmed that the IE protein interacts strongly with TFIIB, but fails to interact with TFIID. IR2, a truncated form of the IE protein lacking the potent transactivation domain and involved in the down-regulation of the IE gene, also interacted with TFIIB but not with TFIID. Studies were also performed to ascertain if particular TBP-associated factors (TAFs) could mediate IE or IR2 binding to TFIID. In vitro transcribed and translated TAF250 added to nuclear extracts generated from EHV-1 infected cells also failed to mediate an interaction between the IE protein or the IR2 protein and TFIID. This study demonstrated that the IE protein mediates transactivation of target viral genes by a mechanism that involves TFIIB. This is in contrast to mechanisms that have been proposed for both the herpes simplex virus ICP4 and VP16 protein which have been proposed to transactivate viral genes through interactions involving both TFIIB and TFIID. This study also intimates that IR2 mediate its repressive effects during the course of EHV-1 infection by a mechanism that involves sequestration of various transcription factors.

Development of Transition Joint for 154kV OF Cable and XLPE Cable (154kV OF-XLPE 이종케이블 접속함의 개발)

  • Kim, H.J.;Kang, B.K.;Song, J.H.
    • Proceedings of the KIEE Conference
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    • 2004.07c
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    • pp.1826-1828
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    • 2004
  • 최근 XLPE 케이블의 신뢰성 향상을 위한 지속적인 연구 및 기술개발이 성과를 나타내면서, 지난 한세기동안 세계적으로 실선로에 적용되어 그 신뢰성을 인정받던 OF 케이블이 XLPE 케이블의 상대적으로 낮은 송전 손실, 높은 방염 특성 및 유지보수와 설치의 편이성에 밀려 그 수요가 급격히 감소하고 있는 추세이다. 이러한 추세에 따라 기존의 OF 케이블 선로의 증설 또는 이설 공사의 경우, 기설 OF 케이블 선로에 XLPE 케이블을 연결하여 사용하는 경향이 많아지며, 이 이종의 케이블을 상호간 연결하는 중간접속함의 필요성이 대두되었다. 이 이종케이블 접속함은 각 케이블의 제반 특성을 최대한 유지하는 동시에 장기적인 신뢰성 확보가 최대 과제이며, 기설의 전력구에 사용하기 위한 경량화 또한 요구된다.

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Electromagnetic Field Analysis on Surge Response of 500 kV EHV Single Circuit Transmission Tower in Lightning Protection System using Neural Networks

  • Jaipradidtham, Chamni
    • 제어로봇시스템학회:학술대회논문집
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    • 2005.06a
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    • pp.1637-1640
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    • 2005
  • This paper presents a technique for electromagnetic field analysis on surge response due to Mid-span back-flashovers effects in lightning protection system of 500 kV EHV single circuit transmission tower by the neural networks method. These analyses are based on modeling lightning return stroke as well as on coupling the electromagnetic fields of the stroke channel to the line. The ground conductivity influences both the electric field as well as the coupling mechanism and hence the magnitude and wave shape of the induced voltage. The technique can be used to analyzed the corona voltage effect, the effective of stroke to the span tower, the surge impedance of transmission lines. The maximum voltage from flashovers effects in the lines. The model is compatible with general electromagnetic transients programs such as the ATP-EMTP. The simulation results show that this study analyses for time-domain with those produced by a cascade multi-section model, the surge impedance of a full-sized tower hit directly by a lightning stroke is discussed.

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A Heat Treatment effect on Breakdown of PET Film for EHV Gas VT (EHV Gas VT용 PET Film 절연파괴에 미치는 열처리 영향)

  • Kim, J.D.;Park, J.Y.;Kim, J.D.;Jung, J.G.;Kim, J.S.;Ha, H.J.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.05b
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    • pp.212-215
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    • 2000
  • This paper presents the temperature effect of PET Film for insulation EHV Gas VT. We measured the Breakdown Voltage (BDV) and the Partial Discharge Initial Voltage(PDIV) for fresh and heated PET film. In this results, The BDV and PDIV of PET Film was affected by temperature variation and the number of PET Film. The PDIV and BDV of PET Film slightly increased for heating treatment increased as the temperature is increased. however, decreased over about $150^{\circ}C$.

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OBSERVATIONS OF CO J = 2 $\to$ 1 AND 3 $\to$ 2 LINES TOWARD EXTREMELY HIGH VELOCITY OUTFLOWS

  • CHOI MINHO
    • Journal of The Korean Astronomical Society
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    • v.29 no.spc1
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    • pp.195-196
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    • 1996
  • We observed CO J = 2 $\to$ 1 and J = 3 $\to$ 2 lines toward several star formation regions with extremely high velocity (EHV) outflows: W3 IRS5, W28 A2, GL2591, S140, and Cepheus A. The full width of the wings are 90-235 km $s^{-1}$. Some wings show clear break of slope in the line profile implying that the nature of the EHV outflow is different from that of the high velocity outflow. We suggest that the EHV CO wing emission is tracing CO molecules in the stellar wind or jet which drives the high velocity outflow.

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Ac Magnetic Field Analysis of Current Carrying Conductor Design for EHV GIS (초고압 GIS 모선의 통전설계를 위한 교류자계해석)

  • Kim, Hyeon-Hun;Lee, Jeong-Seop;Han, Seong-Jin;Jeong, Jin-Gyo;Lee, Byeong-Yun;Park, Gyeong-Yeop
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.48 no.5
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    • pp.233-240
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    • 1999
  • The current carrying conductor and the tank which consist of GIS must be properly designed to withstand the electrical, thermal and mechanical stresses that arise during normal service and during short-circuit conditions. In order to design the current carrying conductor for EHV GIS, it is important to consider temperature-rise when rated current flows. In this paper, we analyze magnetic field distribution and power-loss, according to the change of materials when AC current flows into single-phase and three-phase bus bar, respectively. These results will be used as the basic design data when determining dimensions and materials for the current carrying conductor of EHV GIS.

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Coinfected cases with equine herpesvirus type 1, 4 and Streptococcus equi subsp. zooepidemicus in throughbred horse

  • Kim, Seong-Guk;Cho, Gil-Jae;Cho, Min-Hee;Kim, Young-Hoan;Lee, Hong-Young;Choi, Jeong-Hye;Kim, Jeong-Hwa;Choi, Seong-Kyoon
    • Korean Journal of Veterinary Service
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    • v.34 no.2
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    • pp.187-190
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    • 2011
  • The Thoroughbred horse was an approximately 4-years-old castrated male with highly emaciation, nasal epistaxis and subsequently died. Gross necropsy revealed epistaxis and hyperemia on the lung, multiple hemorrhage in muscle, and liver was focally attached to the peritoneum with fibrin. According to polymerase chain reaction (PCR), Equine herpes virus type 1 and 4 (EHV type 1, 4) was detected in the lung and trachea. In bacterial culture from kidney, liver, spleen, muscle and blood, Streptococcus equi subsp. zooepidemicus was isolated. Based on the gross lesion and PCR, this horse was diagnosed as EHV type 1, 4 and S. zooepidemicus coinfection.