• Polymer(Korea)
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• v.29 no.2
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• pp.135-139
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• 2005

In this study, poly(ethylene imine) (PEI) modified by methoxypoly(ethylene glycol) (mPEG) and folate as a gene carrier was synthesized to decrease cytotoxicity and to improve in vivo targeting. mPEG was modified by glutaric anhydride (GA) to endow carboxylic end group, followed by the activation reaction with EDC (N-ethyl-N'-(3-dimethyl-aminopropyl) carbodiimide) and NHS (N-hydroxysuccinimide). The activated carboxylic end group of mPEG was reacted with the amines of PEI to give mPEG graft PEI. The mPEG-folate-graft-PEI was synthesized by the reaction of mPEG-PEI with folate pre-activated by EDC/NHS. The obtained copolymers were characterized by $^1H-NMR$ and FT-IR. Gel retardation assay and fluorescence measurement indicated that DNA formed the complexes with the synthesized copolymers above N/P charge ratio 2. The size of complexes was ranging from 100 nm to 300 m. In conclusion, we confirmed that the synthesized copolymer have the possibility as a DNA carrier.

• Polymer(Korea)
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• v.29 no.5
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• pp.501-507
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• 2005

Small intestinal submucosa (SIS) had been widely used as a biomaterial without immune rejection responses. SIS sponges prepared by crosslinking with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). SIS powders dissolved in $3\%(v/v)$ acetic acid aqueous solution for 48hrs and freeze-dried. EDC solution ($H_2O$ : ethanol = 5 : 95) as a crosslink agent was used in concentration of 100mM. In vitro, rat-BMSCs seeded in SIS sponges and induced the osteogenesis for 28 days. We have characterized the osteogenic potential of rat-BMSCs in SIS sponges by alkaline phosphatase activity(ALP), n assay, SEM and RT-PCR for osteogenic phenotype. In SEM, all morphology of SIS sponges was regular and showed interconnected pore structure. By RT-PCR analysis, we observed type I collagen expression. These results demonstrate osteogenic differentiation of rat-BMSCs. In conclusion, we confirmed that the morphology of surface, cross-section, and side of SIS sponges were highly porous with good interconnections between each pores, which can support the surface of cell growth, proliferation, and differentiation. This result indicates that SIS sponge is useful for osteogenesis of BMSCs.

• Journal of Cadastre & Land InformatiX
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• v.53 no.2
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• pp.53-66
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• 2023

Recently, there has been a significant interest in the development of autonomous driving simulation environment based on digital twin. In the development of such digital twin-based simulation environment, many researches has been conducted not only performance and functionality validation of autonomous driving, but also generation of virtual training data for deep learning. However, such digital twin-based autonomous driving simulation system has the problem of requiring a significant amount of time and cost for the system development and the data construction. Therefore, in this research, we aim to propose a method for rapidly designing and implementing a digital twin-based autonomous driving simulation system, using only the existing 3D models and high-definition map. Specifically, we propose a method for integrating 3D model of FBX and NGII HD Map for the Busan EDC area into CARLA, and a method for adding and modifying CARLA functions. The results of this research show that it is possible to rapidly design and implement the simulation system at a low cost by using the existing 3D models and NGII HD map. Also, the results show that our system can support various functions such as simulation scenario configuration, user-defined driving, and real-time simulation of traffic light states. We expect that usability of the system will be significantly improved when it is applied to broader geographical area in the future.

• Journal of Sensor Science and Technology
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• v.23 no.5
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• pp.295-298
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• 2014

A novel approach to fabricating high-performance glucose sensors is reported, which is based on the process of self-assembled monolayers (SAMs). In this study, we have particularly used ${\alpha}$-lipoic acid (LA) SAMs for the glucose sensors. To our best knowledge, this study is the first one to use LA as SAMs for this purpose. N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were deliberately attached at the same time on the LA SAM. Then, glucose oxidase ($GO_X$) and horseradish peroxidase (HRP) were sequentially immobilized. Thus, the HRP/$GO_X$/NHS-EDC/LA-SAM/Au/Cr/glass working electrode was developed. The glucose-sensing capability of the fabricated sensor was systematically measured by the use of cyclic voltammetry in the range of 1-30 mM glucose in phosphate-buffered saline. The result showed a good sensitivity, that is, as high as $27.5{\mu}A/(mM{\cdot}cm^2)$. This result conspicuously demonstrates that LA can be one of promising substances for use as SAMs for accurately monitoring trace levels of glucose concentration in human blood.

• Journal of Microbiology and Biotechnology
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• v.26 no.5
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• pp.837-845
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• 2016

A novel endodextranase isolated from Paenibacillus sp. was found to produce isomaltotetraose and small amounts of cycloisomaltooligosaccharides with a degree of polymerization of 7-14 from dextran. To determine the active site, the enzyme was modified with 1-ethyl-3-[3-(dimethylamino)-propyl]-carbodiimide (EDC) and α-epoxyalkyl α-glucosides (EAGs), an affinity labeling reagent. The inactivation followed pseudo first-order kinetics. Kinetic analysis and chemical modification using EDC and EAGs indicated that carboxyl groups are essential for the enzymatic activity. Three Asp and one Glu residues were identified as candidate catalytic amino acids, since these residues are completely conserved across the GH family of 66 enzymes. Replacement of Asp189, Asp340, or Glu412 completely abolished the enzyme activity, indicating that these residues are essential for catalytic activity.

• Fibers and Polymers
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• v.2 no.2
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• pp.64-70
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• 2001

A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its boilogical stability and enhance its mechanical properties. The crosslinking medium has a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.

• Membrane and Water Treatment
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• v.7 no.6
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• pp.507-520
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• 2016

The effect of ZnO on cellulose acetate in the removal of benzophenone-3 (BP-3) was investigated. The benzophenone-3 (BP-3) which is an endocrine disrupting chemical (EDC) was completely removed (100%) from the drinking water using Cellulose Acetate (CA) and zinc oxide (ZnO) composite membranes. The membranes were prepared by DIPS method and the filtration experiments were conducted by dead end filtration unit. The macrostructure of the membrane were studied by ATR-IR and XRD Spectra's. Atomic force microscopy (AFM) and Scanning electron microscopy (SEM) were used to study the micro properties of the membranes. The laboratory experiments such as water uptake study and pure water flux performed to confirm the increasing hydrophilicity. The enhancing hydrophilicity was confirmed with respect to higher the concentration of nanoparticles. Evaluation of BP-3 removal was carried in different experimental conditions, such as, different Trans membrane pressure and different concentration of feed. The membrane with low pressure showed better performance by rejecting 100% of BP-3. However, 1 ppm, 3 ppm and 6 ppm of feed solution was used and among them 3 ppm of feed solution gives 100% rejection. The ZnO nanoparticales enhances the performance of CA membrane by showing maximum rejection.

• Journal of the Earthquake Engineering Society of Korea
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• v.13 no.4
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• pp.25-35
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• 2009

This paper introduces the performance of a newly developed base isolation system made from the combination of a polyurethane disk - attached pot bearing and C-shape steel dampers. Ultimate compressive load tests, ultimate rotation tests, dynamic tests, and dynamic load repeat tests have been completed to determine dynamic characteristics and to verify performance characteristics. The experimental results are compared with the analytic results. It is determined that all requirements for bridge bearing in the specifications are satisfied, and that adequate energy dissipation has occurred. The EDC and effective stiffness estimated by tests are similar to the theoretical values.

• Steel and Composite Structures
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• v.42 no.6
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• pp.791-804
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• 2022

As a type of semi-rigid connection, the top and seat angle connections are popular in current structures owing to their good cyclic performance and simple erection. However, their stiffness and load bearing capacity are relatively insufficient. This study proposes two strengthening methods to further increase the stiffness and strength of bolted-angle joints while maintaining satisfactory energy dissipation capacity (EDC) and ductility. Cyclic loading tests were conducted on six joint specimens with different strengthened angle components. Based on the test results, the influence of the following important factors on the cyclic behavior of steel joint specimens was investigated: the position of the rib stiffeners (edge rib stiffeners and middle rib stiffener), steel strength grade of rib stiffeners (Q345 and Q690), and additional stiffeners or not. In addition, the finite element models of these specimens were built and validated through a comparison of experimental and numerical results. The stiffness and bearing capacity of the bolted-angle joints could be improved significantly by utilizing the novel strengthened joints proposed in this study. Moreover, this can be achieved with almost no increase in the amount of steel required, and the EDC of this joint could also satisfy the requirements of seismic codes from various countries.

• Journal of Distribution Science
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• v.20 no.10
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• pp.93-103
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• 2022

Purpose: This study aims to determine the relationship between disruptive factors and customer satisfaction at chain stores. Survey-based questionnaires were designed in the distribution technique to measure the findings in this study. Research design, data, and methodology: In terms of the sampling technique, the researchers adopted the simple random sampling technique with a total of 200 sample sizes. For the statistical method, the researchers applied multiple linear regression analysis to determine the potential factors that affect customer satisfaction at chain stores. The analysis of this study measured how product quality, pricing policies of chain stores, design and layout, responsiveness, and location of chain stores impart their roles in customer satisfaction. Results: This study found a significant relationship between the product quality and location of chain stores on customer satisfaction. In addition, the responsiveness, pricing policy, and physical design of chain stores impart an insignificant role in customer satisfaction. However, it is proven that the location of chain stores and product quality positively impact customer satisfaction. Conclusions: The study is geographically limited to the region of Karachi, Pakistan. Therefore, the findings may differ in the context of study implications in the other areas.