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Preparation of PEG-Folate-graft-Polyethylenimine as a Gene Carrier  

Seo Dong Hoan (College Pharmacy, Sungkyunkwan University)
Kim Seon Hwa (Department of Advanced Organic Materials Engineering, Chonbuk National University)
Khang GilSon (Department of Advanced Organic Materials Engineering, Chonbuk National University)
Chi Sang Cheol (College Pharmacy, Sungkyunkwan University)
Shin Byung Cheol (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Kim Moon Suk (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
Publication Information
Polymer(Korea) / v.29, no.2, 2005 , pp. 135-139 More about this Journal
Abstract
In this study, poly(ethylene imine) (PEI) modified by methoxypoly(ethylene glycol) (mPEG) and folate as a gene carrier was synthesized to decrease cytotoxicity and to improve in vivo targeting. mPEG was modified by glutaric anhydride (GA) to endow carboxylic end group, followed by the activation reaction with EDC (N-ethyl-N'-(3-dimethyl-aminopropyl) carbodiimide) and NHS (N-hydroxysuccinimide). The activated carboxylic end group of mPEG was reacted with the amines of PEI to give mPEG graft PEI. The mPEG-folate-graft-PEI was synthesized by the reaction of mPEG-PEI with folate pre-activated by EDC/NHS. The obtained copolymers were characterized by $^1H-NMR$ and FT-IR. Gel retardation assay and fluorescence measurement indicated that DNA formed the complexes with the synthesized copolymers above N/P charge ratio 2. The size of complexes was ranging from 100 nm to 300 m. In conclusion, we confirmed that the synthesized copolymer have the possibility as a DNA carrier.
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