• 대한바이러스학회지
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• 제29권3호
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• pp.165-174
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• 1999

To investigate viral pathogenesis and in vivo efficacy of acyclovir (ACV) in mouse HSV-1 encephalitis models, female BALB/c mice aged 5 weeks were inoculated with strain F either intranasally (IN) or intracerebrally (IC). ACV-treatment by intraperitomeal injection with 0, 5, 10 and 25 mg/kg b.i.d. for 6 days was commenced 1 h after infection. Body weight and signs of clinical disease were noted daily up to 2 weeks. $ED_{50}$ of ACV in IN infection was <5 mg/kg and 14.1 mg/kg in IC infection. Tissues of central nervous system were collected from 2 mice per group everyday up to 5 day p.i. and the virus titers were measured. In IN infection model, high titers in eyes and trigeminal nerves were observed. ACV-treatment showed significant reduction of the titers in all the isolated. In IC infection model, cerebrum, cerebellum and brain stem showed high virus titers. ACV-treatment showed less significant reduction of virus titers than that in IN infection model. Reactivation of explanted trigeminal nerves from mice 30 day p.i. was monitored. In all of ACV treated mice reactivation was observed, i.e. even the highest dose of ACV did not inhibit the establishment of viral latency.

• 대한수의학회지
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• 제40권3호
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• pp.431-437
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• 2000

The aim of this study was to evaluate the involvement of CPP32 (caspase-3), one of the death-related enzymes, in the course of experimental autoimmune encephalomyelitis (EAE). EAE was induced in Lewis rats immunized with an emulsion of rat spinal cord homogenate with complete Freunds adjuvant supplemented with Mycobacterium tuberculosis (H37Ra, 5mg/ml). The expression of CPP32 in the spinal cords of rats with EAE was studied. In normal rat spinal cords, CPP32 is constitutively, but weakly, expressed in neurons and some neuroglial cells. In the EAE spinal cords, many inflammatory cells were positive for CPP 32, and the majority of CPP32(+) cells were identified as ED1(+) macrophages. During this stage of EAE, the number of CPP32(+) cells in brain cells, including neurons and astrocytes, increased, and these cells also had increased CPP32 immunoreactivity. CPP32 immunor eactivity was not always matched with apoptosis of inflammatory cells in EAE lesions. We speculate that CPP32, which is constitutlvely expressed in brain cells, increases in response to neuroimmunological stimulation in both brain neuronal cells and inflammatory cells. The functional role of CPP32 in neuroimmunological disorders is discussed.

• 한국재료학회지
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• 제10권5호
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• pp.321-327
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• 2000

다공성 알루미나 소결체내부로 3Y-TZP 및 12Ce-TZP 전구체를 각각 액상침투시킴으로써 2종류의 $Al_2O_3/TZP$복합체를 제조하였다. 소량의 (~11.0 wt%) TZP의 첨가는 Al2O3소결체 ($1600^{\circ}C$, 2시간)의 강도 (19~59%)와 파괴인성(14~157%)을 증가시켰다. 3Y-TZP의 첨가는 복합체의 강도의 향상에 12Ce-TZP의 첨가는 인성의 향상에 보다 효과적이었다. 침투도니 TZP는 복합체의 내부보다 표면에 집중되었으며, 그 결과 이곳에서의 입성장에 빨랐고 $Al_2O_3$의입성장 억제효과도 상대적으로 뛰어났다. 입계 및 입내균열전파가 일어났으나 $Al_2O_3/12Ce-TZP$의 경우가 $Al_2O_3/3Y-TZP$에 비하여 입계파괴가 우세하였다

• 보존과학회지
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• 제17권
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• pp.57-64
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• 2005

청평사 강선루 회전문 부근에서 출토된 조선시대 $16\~17$세기 철물과 철촉에 대한 금속조직학적으로 조사하기 위하여 유물에서 부식되지 않은 시편을 채취하여 마운트와 연마 및 부식을 시켜 금속조직을 관찰하고 비금속개재물부분은 SEM으로 관찰한 뒤 EDS로 분석하였다. 금속학적 조직조사와 SEM-EDS분석을 통하여 철물과 철촉은 고체저온환원법에 의한 순철에 가까운 괴련철 혹은 해면철을 이용하여 철을 생산하고, 단야로 같은 곳에서 침탄을 하여 탄소량을 높인 것으로 밝혀졌다. 또한 철물은 탄소량을 어느 정도 높인 다음 담금질과 열처리과정을 반복하여 철기의 강도를 인위적으로 많이 높이는 작업을 한 것으로 밝혀졌다. 이번 조사연구를 통하여 조선시대에도 괴련철을 소재로 하여 철을 생산하였다는 것을 알 수 있어 의미 있는 결과를 얻었다.

• 수산해양기술연구
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• 제23권1호
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• pp.30-38
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• 1987

좁은 틈새를 가진 원판밸브의 추력에 대한 이론적 해석 및 실험 결과로부터 다음과 같이 요약할 수 있다. 1. 원판 밸브의 틈새가 비교적 작은 경우(H/D sub(1)가 0.1 이하) 즉, 유량이 적을 때에는 점성류가 지배적이고 이론식으로부터 추력을 높게 예측할 수 있었다. 2. 압력의 영향에 대한 추력은 입구 압력이 낮을수록 이론결과와 실험결과가 잘 일치하고 높을수록 관성의 영향이 크게 나타나는 경향을 보였다. 3. 밸브 크기 변화에 대한 추력의 영향은 D sub(2)/D sub(1)=>4.6에서는 포물선적으로 감소하고 그 이하에서는 직선적으로 감소하였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 제37회 하계학술대회 논문집 D
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• pp.2134-2135
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• 2006

전력거래소는 발전경쟁시장(CBP; Cost-Based Pool) 장기화에 따른 운영상의 효율성을 개선하고 기 개발된 시장운영시스템(MOS; Market Operation System)을 활용하여 급전체계를 개선하기 위해 준비중이다. 현행 급전체계에서는 거래 전일에 수행한 수요예측을 바탕으로 1시간 단위운영발전계획을 전일에 수립하고 EMS(Energy Management System)를 이용하여 발전기에 대한 경제부하배분(ED; Economic Dispatch)을 시행하고 있지만, 현 EMS는 시장체제 환경 전에 도입된 설비로 시장환경에 대한 고려가 되어 있지 않고 계통운영 보조서비스의 실시간 반영이 어려운 점이 있다. 전력거래소는 실시간 급전 운영을 위해 기존 EMS에 MOS를 연계하여 MOS의 5분 단위 수요예측량을 기반으로 송전망 제약과 예비력 요구량 등을 고려한 발전기별 경제부하 배분량 및 예비력 배분량을 결정하고, 추가적으로 EMS에서 수요예측 오차 및 주파수 보정량을 실시간으로 계산하여 발전기별로 배분하도록 함으로써, 1일 전 시행하던 급전계획을 취득 자료를 기반으로 5분 단위로 실시간 계산할 수 있도록 급전 체계를 개선할 계획이다. 이를 통해 실시간으로 에너지와 예비력을 동시에 최적화함으로 전력시장 및 전력계통 운영을 한층 선진화 할 수 있는 계기를 마련하였으며 또한 저비용 발전기 사용을 극대화함으로 발전비용의 절감에도 기여하는 효과를 기대할 수 있다. MOS-EMS간 자료연계에는 ICCP(Inter-Control Communication Protocol)와 FTP 프로토콜을 사용하였고, 수차례 모의운영을 통하여 데이터베이스 및 현장 취득 자료의 정확도(accuracy)가 양 시스템 간 연계 및 전력 계통의 안정적 운영에 매우 중요한 요소로 나타났다. 전력거래소는 장기적으로 CIM(Common Information Model)기반의 표준 전력계통 데이터베이스를 구축하고 시스템 간 자료 연계를 위해 XML을 활용하여 시스템 간 상호 운영성(Interoperability)과 자료 연계의 안정성을 높일 계획이다. 본 논문은 MOS-EMS 연계에 따른 시스템 간 자료의 흐름 및 처리에 대해 주로 설명하고자 한다.

• The Journal of Advanced Prosthodontics
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• 제5권3호
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• pp.226-233
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• 2013

PURPOSE. This study evaluated the adhesion of 10-MDP containing self-etch and self-adhesive resin cements to dentin with and without the use of etch-and-rinse technique. MATERIALS AND METHODS. Human third molars (N=180) were randomly divided into 6 groups (n=30 per group). Conventional (Panavia F2.0, Kuraray-PAN) and self-adhesive resin cements (Clearfil SA, Kuraray-CSA) were bonded to dentin surfaces either after application of 3-step etch-and-rinse (35% $H_3PO_4$ + ED Primer) or two-step self-etch adhesive resin (Clearfil SE Bond). Specimens were subjected to shear bond strength test using the universal testing machine (0.5 mm/min). The failure types were analyzed using a stereomicroscope and quality of hybrid layer was observed under a scanning electron microscope. The data (MPa) were analyzed using two-way ANOVA and Tukey's tests (${\alpha}$=.05). RESULTS. Overall, PAN adhesive cement showed significantly higher mean bond strength ($12.5{\pm}2.3-14.1{\pm}2.4$ MPa) than CSA cement ($9.3{\pm}1.4-13.9{\pm}1.9$ MPa) (P<.001). Adhesive failures were more frequent in CSA cement groups when used in conjunction with two-step self-adhesive (68%) or no adhesive at all (66%). Hybrid layer quality was inferior in CSA compared to PAN cement in all conditions. CONCLUSION. In clinical situations where bonding to dentin substrate is crucial, both conventional and self-adhesive resin cements based on 10-MDP can benefit from etch-and-rinse technique to achieve better quality of adhesion in the early clinical period.

• International Journal of CAD/CAM
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• 제6권1호
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• pp.9-17
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• 2006

Performing inspections for Hull Condition Monitoring and Assessment as stipulated in IACS unified requirements and IMO's Condition Assessment Scheme (CAS) IMO Resolution MEPC.94(46), 2001, Condition Assessment Scheme, IMO Resolution MEPC.111(50), 2003, Amendments to regulation 13G, addition of new regulation 13H involves a huge amount of measurement data to be collected, processed, analysed and maintained. Information to be recorded consists of thickness measurements and visual assessment of coating and cracks. The amount of data and increasing requirements with respect to condition assessment demand efficient computer support. Currently, due to the lack of standardization for this kind of data, the thickness measurements are recorded manually on ship drawings or tables. In this form, handling of the measurements is tedious and error-prone and assessment is difficult. Data reporting and analysis takes a long time, leading to some repairs being performed only at the next docking of the ship or making an additional docking necessary. The recently started ED funded project CAS addresses this topic and develops-as a first step-a data model for Hull Condition Monitoring and Assessment (HCMA) based on XML-technology. The model includes simple geometry representation to facilitate a graphically supported data collection as well as an easy visualisation of the measurement results. In order to ensure compatibility with the current way of working, the content of the data model is strictly confined to the requirements of the measurement process. Appropriate data interfaces to classification software will enable rapid assessment by the classification societies, thus improving the process in terms of time and cost savings. In particular, decision-making can be done while the ship is still in the dock for maintenance.

• Journal of Advanced Marine Engineering and Technology
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• 제22권5호
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• pp.660-669
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• 1998

In this paper the Hardness and Electro-chemical corrosion of the Nimonic 80A superalloy were studied. It aging heat treatments was carried out at $650^{\circ}C$, $700^{\circ}C$, $750^{\circ}C$,$800^{\circ}C$ and $850^{\circ}C$ with different time of 20min , 30min 1hour, 2hours, 4hours, and 16hours additionally 64hours and 128hours at $650^{\circ}C$. The obtained results were as follows; 1. As aging temperature increased the time for the maximum hardness was reduced from 128hours at $650^{\circ}C$ to 30min at $850^{\circ}C$ whereas the highest hardness was reduced from Hv 381 at $650^{\circ}C$ to Hv 321 at $850^{\circ}C$. 2. In the Electro-chemical corrosion test as a function of aging heat treatment time and tem-perature the corrosion potential was reversely proportional to Hardness which indicated the effects of ${\gamma}/{\gamma}'$ coherency of base material and precipitate. 3. Initiation point of the pitting was observed at grain boundary twin boundary and near${\gamma}'$ pre-cipitates. The results of composition analysis by EDS at this point indicated that sulphur originat-ed from 1N $H_2SO_4$ solution was found in depletion at the grain boundaries and the pit which arouse in the near precipitates were lack of Al Ti and Ni which are the main element of ${\gamma}'$ The depletion of such element was cause breakdown of passive film.

• Journal of Microbiology and Biotechnology
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• 제1권2호
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• pp.116-120
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• 1991

The transformant Bacillus subtilis ED213 carrying the pSO100 which cloned the cdd gene encoding cytidine deaminase (cytidine /2'-deoxycytidine aminohydrolase, EC 3.5.4.5, CDase) originated from wild type B. subtilis was cultivated in Spizizen minimal medium (SMM). To overcome poor expression of the cdd gene in SMM medium, the medium compositions and growth conditions were optimized. The optimized medium compositions and growth conditions were cytidine concentration of 80 mg/l, glycerol of 25 g/l, and $(NH_4)_2SO_4$ of 10 g/l, along with $37^{\circ}C$ and pH 7.0. The intracellular CDase production was increased 3 times from 1,000 unit/ml to 3,200 unit/ml, and extracellular CDase also increased from nearly undetectable amounts to 1,500 unit/ml. The cytidine concentration was signified as the most critical compositional factor for overproduction of CDase by increasing the cell density mainly in culture broth. The plasmids were more stable in cells that were grown in original SMM medium with stability of 90% compared to those grown in optimized SMM medium with stability of 80% after 48 hours cultivation. The most active amplification of plasmid was occurred in the logarithmic phase, which showed a value around four times higher than the initial copy number. In the exponential phase, the CDase production was closely related to the plasmid copy number along with the cell density. However, it was not accorded with cell density at the stationary phase.