• The Korean Journal of Zoology
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• v.33 no.3
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• pp.337-345
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• 1990

The properties of three esterases (E2, E6 and E11) which were previously purified from Pieris rapae L. were determined and physiological role of E6 was inferred using immunological methods. Based on inhibitor study, all of the purified esterases were found to be carboxylesterases (EC 3.1.1.1). The Km values for E2, E6 and E11 were determined to be 6.89 X 10-$^4$M. 3.19 $\times$ l0-$^4$M and 3.69 X 10-$^4$M, respectively. The molecular weights of E2, E6 and E11 were estimated to be 42 KD, 81 KD and 174 KD, respectively. The isoelectric points of E2, E6 and E11 were estimated to be pH 5.54, pH 5.89 and pH 6.50, respectively. The concentration of E6 during development was highest at the late 5th instar larval stage and that according to organs at the same stage was highest in midgut. These results suggest that E6 might be a hydrolase involved in the digestion of dietary lipids.

• Microbiology and Biotechnology Letters
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• v.19 no.5
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• pp.491-496
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• 1991

To maximize the production of aminoglycoside-3'-phosphotransferase of E. coli ATCC 21990 carrying R factor which encodes aminoglycoside-3'-phosphotransferase (APH(3')) phosphorylating the 3'-hydroxyl group of aminoglycoside, mutants M1 and M2, media composition and several factors affecting the enzyme production during fermentation were studied. Although the specific activity of APH(3') produced by a mutant M1 was increased as much as four times than that of E. coii ATCC 21990, the growth rate was decreased. The increase of the enzyme production was obtained by increased biomass during fermentation. A mutant M2 was obtained to increase the cell growth rate. Mutant M2 cells were cultivated with optimal media and pure oxygen gas in a fed-batch mode of fermentor operation. The specific activity of APH(3') was decreased, but total enzyme activity of APH(3') was increased as much as two point five times than that of mutant MI.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.157-162
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• 2009

As the improvement of computer performance and the development of IT technology have influence upon publishing industry, the e-Book system, a type of electronic resources, has come out. This is a method to use both texts and 2D image illustrations. Nowadays, there are more and more users who want high quality contents and a variety of contents such as the illustrated fish book using the 3D system. This article adds 3D animation objects to the current e-Book system and designs and realizes the 3D fish simulation system to which e-Book technology is applied so as to improve the comprehension of texts and readability of detailed information and increase readers' immersion as well.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.17 no.2
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• pp.59-63
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• 1981

For the purpose of surveying accuracy of the position fix and local fluctuation of Omega signals in south coast of Korea, the author observe continuously the phase difference of the A-C pair and A-E pair form December 1, 1980, to February 28, 1981, at Cheju(33$^{\circ}$31'.0N, 126$^{\circ}$31.9E) and Busan(35$^{\circ}$7'.9 N, 129$^{\circ}$6'. 3 E). The analyzed results are as follows: 1. Omega signals at Cheju and Busan are positive correlation. Mean value of coefficients during all measured time is 0.70 with A-C pair and 0.75 with A-E pair. 2. Range of mean value of difference between the measured PPC at Cheju and Busan is 17.7~21.3 cels with A-C pair and -2.9~2.0 cels with A-E pair. 3. In case of using the differential Omega at Cheju and Busan, mean value of total errors of the position fix is 1.52 miles and its standard deviation is 0.21 miles.

• Molecules and Cells
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• v.42 no.12
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• pp.840-849
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• 2019

The spatiotemporal mitotic processes are controlled qualitatively by phosphorylation and qualitatively by ubiquitination. Although the SKP1-CUL1-F-box protein (SCF) complex and the anaphase-promoting complex/cyclosome (APC/C) mainly mediate ubiquitin-dependent proteolysis of mitotic regulators, the E3 ligase for a large portion of mitotic proteins has yet to be identified. Here, we report c-Cbl as an E3 ligase that degrades DDA3, a protein involved in spindle dynamics. Depletion of c-Cbl led to increased DDA3 protein levels, resulting in increased recruitment of Kif2a to the mitotic spindle, a concomitant reduction in spindle formation, and chromosome alignment defects. Furthermore, c-Cbl depletion induced centrosome over-duplication and centriole amplification. Therefore, we concluded that c-Cbl controls spindle dynamics and centriole duplication through its E3 ligase activity against DDA3.

• Communications of the Korean Mathematical Society
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• v.35 no.2
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• pp.469-480
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• 2020

Let R be a commutative ring with identity and M a unital R-module. We give a new generalization of exact sequences called e-exact sequences. A sequence $0{\rightarrow}A{\longrightarrow[20]^f}B{\longrightarrow[20]^g}C{\rightarrow}0$ is said to be e-exact if f is monic, Imf ≤_e Kerg and Img ≤_e C. We modify many famous theorems including exact sequences to one includes e-exact sequences like 3 × 3 lemma, four and five lemmas. Next, we prove that for torsion-free module M, the contravariant functor Hom(-, M) is left e-exact and the covariant functor M ⊗ - is right e-exact. Finally, we define e-projective module and characterize it. We show that the direct sum of R-modules is e-projective module if and only if each summand is e-projective.

• Korean Journal of Animal Reproduction
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• v.15 no.1
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• pp.15-22
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• 1991

To determine the preventive effects of selenium and/or vitamin E on placenta retention in Holstein dairy cows. The cows were divided six groups ; control, Se, vit.E 1x, vit.E 3x, Se + vit./E 1x and Se + vit.E 3x in Cooong Nam province. 50mg of Se as sodium selenite and 700 IU of vit. E as dl-${\alpha}$ tocopherol acetate were injected 21 day perior to expected calving date, and 700 IU vit.E was injected 3 times at 21, 14 and 7 days perior to expected calving date in vit.E 3x groups. The cows which did not expelled their placenta until 12 hours post-parturient were termed as cow of placenta retention. The i.m. injection of a single dose of selenium and vit.E has significantly(P<0.05) affected the incidence of retained placenta by 7.0% versus 25.6% of controls, but a single injection of selenium or vit.E and a combination of selenium 1x and vit.E 3x has not significantly affected. But they affected neiher subsequent reproductive performances nor milk yields.

• Journal of Microbiology
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• v.34 no.4
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• pp.341-348
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• 1996

The regulation of xylE gene expression was examined by using vector promoter and construction of genetically engineered microorganisms (GEMs) for application in microcosm. When the xylE gene wsa subcloned into pBluscript SK(+) under the control of lac promoter (pTY1) in E. coli, and the expression was induced by IPTG, the enzyme activity of catechol 2, 3-dioxygenase was increased 4.7 times more than that of the crude extracts from transformants harboring pTY1. We suggest that the xylE gene has its own promoter at the upstream portion, because it was able to be expressed even in the absence of IPTG. A recombinant plasmid, pSW3a harboring the xylE gene under the T7 promotor, showed the activity of 14.5 units/mg protein, higher than that of parental strain, E. coli PYT1. The xylE gene in recombinant plasmid pSW3a was used as reporter gene for the application in microcosm ecosystem, since it was used for detection of xylE-positive clones by catechol spray on the agar plates. The pSW3a in E. coli was introduced into Pseudomonas patida to construct GEM strain, and examined for the exxpression and functional stability in microcosms.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.11 no.2
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• pp.9-14
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• 2007

A signed 3-partite graph is a 3-partite graph in which each edge is assigned a positive or a negative sign. Let G(U, V, W) be a signed 3-partite graph with $U\;=\;\{u_1,\;u_2,\;{\cdots},\;u_p\},\;V\;=\;\{v_1,\;v_2,\;{\cdots},\;v_q\}\;and\;W\;=\;\{w_1,\;w_2,\;{\cdots},\;w_r\}$. Then, signed degree of $u_i(v_j\;and\;w_k)$ is $sdeg(u_i)\;=\;d_i\;=\;d^+_i\;-\;d^-_i,\;1\;{\leq}\;i\;{\leq}\;p\;(sdeg(v_j)\;=\;e_j\;=\;e^+_j\;-\;e^-_j,\;1\;{\leq}\;j\;{\leq}q$ and $sdeg(w_k)\;=\;f_k\;=\;f^+_k\;-\;f^-_k,\;1\;{\leq}\;k\;{\leq}\;r)$ where $d^+_i(e^+_j\;and\;f^+_k)$ is the number of positive edges incident with $u_i(v_j\;and\;w_k)$ and $d^-_i(e^-_j\;and\;f^-_k)$ is the number of negative edges incident with $u_i(v_j\;and\;w_k)$. The sequences ${\alpha}\;=\;[d_1,\;d_2,\;{\cdots},\;d_p],\;{\beta}\;=\;[e_1,\;e_2,\;{\cdots},\;e_q]$ and ${\gamma}\;=\;[f_1,\;f_2,\;{\cdots},\;f_r]$ are called the signed degree sequences of G(U, V, W). In this paper, we characterize the signed degree sequences of signed 3-partite graphs.

• The Journal of Korean Society of Virology
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• v.27 no.1
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• pp.9-17
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• 1997

Hepatitis C virus (HCV) E2 protein is known to be one of putative envelope proteins. To develop a sensitive detection method for HCV infected tissues and cells, monoclonal antibodys (MAbs) to the E2 protein of HCV were prepared from mice immunized with recombinant baculovirus-expressing E2 protein (Bac-E2). Several hybridoma clones secreting various levels of MAb were isolated and isotypes of these MAb were determined. One clone (L.2.3.3) was used for ascites production and the E2-MAb was purified and characterized. The L.2.3.3 reacted well with both Bac-E2 and E. coli expressed glutathione-S-transferase-E2 (GST-E2) fusion proteins. Using HCV patient sera, E2 envelope protein was found to be localized in the cell membrane boundary both in CHO cells and insect cells which express HCV E2 protein. Similar result was obtained when same cells were treated with the MAb L.2.3.3. These results demonstrated that Bac-E2 protein is capable of eliciting high titer antibody production in mice.