• Genomics & Informatics
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• 제21권3호
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• pp.32.1-32.11
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• 2023

Resistance to anti-tuberculosis drugs, especially ethambutol (EMB), has been widely reported worldwide. EMB resistance is caused by mutations in the embB gene, which encodes the arabinosyl transferase enzyme. This study aimed to detect mutations in the embB gene of Mycobacterium tuberculosis from Papua and to evaluate their impact on the effectiveness of EMB. We analyzed 20 samples of M. tuberculosis culture that had undergone whole-genome sequencing, of which 19 samples were of sufficient quality for further bioinformatics analysis. Mutation analysis was performed using TBProfiler, which identified M306L, M306V, D1024N, and E378A mutations. In sample TB035, the M306L mutation was present along with E378A. The binding affinity of EMB to arabinosyl transferase was calculated using AutoDock Vina. The molecular docking results revealed that all mutants demonstrated an increased binding affinity to EMB compared to the native protein (-0.948 kcal/mol). The presence of the M306L mutation, when coexisting with E378A, resulted in a slight increase in binding affinity compared to the M306L mutation alone. The molecular dynamics simulation results indicated that the M306L, M306L + E378A, M306V, and E378A mutants decreased protein stability. Conversely, the D1024N mutant exhibited stability comparable to the native protein. In conclusion, this study suggests that the M306L, M306L + E378A, M306V, and E378A mutations may contribute to EMB resistance, while the D1024N mutation may be consistent with continued susceptibility to EMB.

• International Journal of Control, Automation, and Systems
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• 제3권4호
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• pp.533-541
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• 2005

In this paper, a wind turbine with power splitting transmission, which is realized through a novel three-shaft planetary, is presented. The input shaft of the transmission is driven by the rotor of the wind turbine, the output shaft is connected to the grid via the main generator (asynchronous generator), and the third shaft is driven by a control motor with variable speed. The dynamic models of the sub systems of this wind turbine, e.g. the rotor aerodynamics, the drive train dynamics and the power generation unit dynamics, were given and linearized at an operating point. These sub models were integrated in a multidisciplinary dynamic model, which is suitable for control syntheses to optimize the utilization of wind energy and to reduce the excessive dynamic loads. The important dynamic behaviours were investigated and a wind turbine with a soft main shaft was recommend.

• Bulletin of the Korean Chemical Society
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• 제33권2호
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• pp.589-596
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• 2012

In this paper, the reactive dynamics properties of the reactions Ba + $C_6H_5Br$ and Ba + m-$C_6H_4CH_3Br$ were studied by means of the quasi-classical trajectory method based on the London-Eyring-Polanyi-Sato potential energy surfaces. The vibrational distributions, reaction cross sections, rotational alignments of the products BaBr all were obtained. The peak values of the vibrational distributions are located at $\nu$ = 0 for the reactions Ba + $C_6H_5Br$ and Ba + m-$C_6H_4CH_3Br$ when the collision energies are 1.09 and 1.10 eV, respectively. The reaction cross sections increase with the increasing collision energy, which changes from 0.6 to 1.5 eV. The product rotational alignments deviate from -0.5 and firstly increase and then decrease while the collision energy is increasing, just like that of Heavy+Light-Light system.

• 천문학회지
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• 제28권1호
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• pp.97-107
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• 1995

The stability of the geometrically thin, two-temperature hot accretion disk is studied. The general criterion for thermal instability is derived from the linear local analyses, allowing for advective cooling and dynamics in the vertical direction. Specifically, classic unsaturated Comptonization disk is analysed in detail. We find five eigen-modes: (1) Heating mode grows in thermal time scale, $(5/3)({\alpha}{\omega})^{-1}$, where alpha is the viscosity parameter and w the Keplerian frequency. (2) Cooling mode decays in time scale, $(2/5)(T_e/T_i)({\alpha}{\omega})^{-1}$, where $T_e\;and\;T_i$ are the electron and ion temperatures, respectively. (3) Lightman-Eardley viscous mode decays in time scale, $(4/3)(\Lambda/H)^2({\alpha}{\omega})^{-1}$, where $\Lambda$ is the wavelength of the perturbation and H the unperturbed disk height. (4) Two vertically oscillating modes oscillate in Keplerian time scale, $(3/8)^{1/2}\omega^{-1}$ with growth rate $\propto\;(H/\Lambda)^2$. The inclusion of dynamics in the vertical direction does not affect the thermal instability, adding only the oscillatory modes which gradually grow for short wavelength modes. Also, the advective cooling is not strong enough to suppress the growth of heating modes, at least for geometrically thin disk. Non-linear development of the perturbation is followed for simple unsaturated Compton disk: depending on the initial proton temperature perturbation, the disk can evolve to decoupled state with hot protons and cool electrons, or to one-temperature state with very cool protons and electrons.

• 한국해양학회지:바다
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• 제21권3호
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• pp.103-111
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• 2016

대규모 교란현상 이후 형성된 대형갈조류 감태 개체군의 계절적 변동과 회복양상을 이해하기 위해서 감태의 형태학적 특성, 가입, 사망률, 밀도와 생물량을 2013년 6월부터 2015년 6월까지 조사하였다. 감태의 전체 길이, 가장 긴 측엽의 길이와 개체당 무게는 뚜렷한 계절적 경향성을 보였다. 줄기부의 길이는 겨울부터 봄까지 증가하였으나, 여름부터 가을까지는 변화가 없었다. 이러한 결과는 감태의 형태적 변화가 주로 엽상부의 변화에 의해 나타난다는 것을 의미한다. 감태의 성장은 수온이 $15{\sim}18^{\circ}C$인 겨울부터 봄까지 매우 활발하게 일어나지만, $20^{\circ}C$ 이상의 수온에서는 저해되는 것으로 나타났다. 가입은 실험이 진행되는 기간 동안 봄부터 여름에 걸쳐 매우 낮았다. 그러나 2015년 4월 생육밀도의 감소로 인해 감태의 피도가 매우 낮아진 시점에 대량의 가입이 발생하였으며, 이것은 감태의 가입이 계절적 요인 보다는 공간과 차광효과 같은 물리적 요인에 의해 조절된다는 것을 시사한다. 어린 개체는 부착기질의 불안정성으로 인해 매우 높은 사망률을 보였다. 태풍 '볼라벤'에 의한 교란 이후 34개월이 되는 2015년 6월에 이르러 개체군의 구조가 교란 이전 수준으로 회복되었다. 따라서 대규모 교란현상 이후 감태 개체군이 교란 이전의 개체군 구조로 회복되는 데에는 3년 이상의 시간이 소요되는 것으로 판단된다. 이 연구는 해중림을 구성하는 대형갈조류의 관리, 복원 및 보호 전략을 수립하는데 매우 귀중한 생태학적 자료를 제공할 것이다.

• Ocean and Polar Research
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• 제35권3호
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• pp.205-217
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• 2013

We investigated the population dynamics of Aurelia aurita in Sihwa Lake from April to October in 2009. Salinity ranged from 5.9 to 30.7 psu at the surface. Abundance of mesozooplankton ranged from 3 to 111,874 indiv. $m^{-3}$. Ephyrae occurred from April to May with the peak in abundance occurring on 17 April. Maximum density of ephyrae was observed near the power transmission towers that are known to be habitats of polyps. Mortality of ephyrae was lower than in other areas because of the abundant prey concentration and the absence of predators. Young medusae occurred from April to July with the peak in abundance occurring on 8 May. Adult medusae occurred from May to July with the peak in abundance on 25 June and they disappeared before the rainy season. Planula occurred only in May and June with the peak in abundance on 25 June. Growth rates of Aurelia aurita ranged from -0.06 to 0.34 $d^{-1}$, and decreased rapidly after May. The period in which adult medusa occurred was restricted, compared with those in other area in Korea (e.g., Masan Bay) and Japan (e.g., Tokyo Bay). In the period of this study, the available food was limited in June and salinity decreased to ca. 20 psu in May because of the beginning of the wet season. We assumed that the exceptionally short period of occurrence of the medusa may be a response of adults to changes in temperature, salinity or food limitation which leads to the precocious maturation of young medusa and the release of planula and that the brief occurrence of medusa was caused by an abrupt decrease in activity after the release of planula.

• BMB Reports
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• 제51권3호
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• pp.143-150
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• 2018

The Hippo signaling pathway plays an essential role in adult tissue homeostasis and organ size control. Abnormal regulation of Hippo signaling can be a cause for multiple types of human cancers. Since the awareness of the importance of the Hippo signaling in a wide range of biological fields has been continually grown, it is also understood that a thorough and well-rounded comprehension of the precise dynamics could provide fundamental insights for therapeutic applications. Several components in the Hippo signaling pathway are known to be targeted for proteasomal degradation via ubiquitination by E3 ligases. ${\beta}-TrCP$ is a well-known E3 ligase of YAP/TAZ, which leads to the reduction of YAP/TAZ levels. The Hippo signaling pathway can also be inhibited by the E3 ligases (such as ITCH) which target LATS1/2 for degradation. Regulation via ubiquitination involves not only complex network of E3 ligases but also deubiquitinating enzymes (DUBs), which remove ubiquitin from its targets. Interestingly, non-degradative ubiquitin modifications are also known to play important roles in the regulation of Hippo signaling. Although there has been much advanced progress in the investigation of ubiquitin modifications acting as regulators of the Hippo signaling pathway, research done to date still remains inadequate due to the sheer complexity and diversity of the subject. Herein, we review and discuss recent developments that implicate ubiquitin-mediated regulatory mechanisms at multiple steps of the Hippo signaling pathway.

• 한국수소및신에너지학회논문집
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• 제25권3호
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• pp.234-239
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• 2014

To study catalytic activity and hydrogen embrittlement of Pd, Pt, Ni, and Cr in fuel cell electrode, we used density-functional theory. The calculation tools based electron density give much shorter calculation time and cheap costs. Maximum of bond overlap populations of each metal are 0.6539eV for Pd-H, 0.6711eV for Pt-H, 0.6323eV for Ni-H, 0.6152eV for Cr-H. Electron density of Cr has strongest in related metals, which shows strong localization of electron, implying anti hydrogen embrittlement behaviors.

• 한국재난정보학회:학술대회논문집
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• 한국재난정보학회 2017년 정기학술대회
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• pp.190-191
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• 2017

본 연구는 산림 공간에서 산불소화시설인 수관수막타워의 방수시간에 따른 화재에 미치는 영향을 FDS(Fire Dynamics Simulator, 화재시뮬레이션)로 비교하였다. 본 연구를 진행하기 위한 기본 조건은 수관수막타워 높이 20m, 방수길이 40m, 방수량 400Lpm으로 해석공간 상에 수막타워를 3기 설치하였다. 수관수막타워 작동 초반 화재온도 $668.5^{\circ}C$에서 작동 450초 후 $145.6^{\circ}C$으로 감소되는 효과를 볼 수 있다. 이는 '산불소화시설 설치 및 운영 관리 지침'의 제3조 11절 6항의 '수관수막설비는 산불 접근 예상시간보다 10분전에 가동되도록 해야 한다.'의 타당성을 마련할 수 있다.

• 한국응용곤충학회지
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• 제48권3호
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• pp.319-325
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• 2009

사과면충은 지하부 대목부 및 흡지에서 성충 및 약충 상태로 월동하였고, 약제를 살포하지 않은 사과원의 주간부에서 콜로니는 4월 중순부터 증가하기 시작하여 6월 하순에서 7월 상순 1차 발생최성기를 보였고, 그 후 감소하였다가 7월 하순 2차 발생최성기를 보였으며, 9월 하순에도 1차 발생최성기 규모의 발생량을 보였다. 발생량은 주 당 3.5개를 넘지 않았으며, 연간 2개 내외로 유지되었다. 사과면충좀벌 기생률은 지제부 보다는 지상부에서 기생률이 높았고, 사과면충 발생농가 조사결과 기생률이 최고 70% 이상이었다. 사과면충에 대한 약제방제 효과검정 결과 fenitrothion, dichlorphos, machine oil, methidathion, thiacloprid, imidacloprid 등 약제의 살충률은 각각 97.8, 96.8, 95.4, 91.5, 26.7 및 7.8% 이었다. 또한 동일한 약제에 대한 사과면충좀벌 머미의 성충으로 우화율은 각각 51.2, 72.6, 14.2, 3.5, 72.2 및 85.4% 이었다. 최근 많이 사용하는 진딧물 적용약제들의 살충률은 낮았으며, fenitrothion 및 dichlorphos는 사과면충에 대한 살충률이 높고 천적에 비교적 저독성이어서 종합관리 시 동시방제 약제로 적당하였다.