• Weed & Turfgrass Science
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• v.5 no.4
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• pp.191-195
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• 2016

Tefuryltrione is a new hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor, which has been recently registered for the use for paddy rice, Korea. Dose-response studies were conducted to compare rice safety and weed control efficacy of tefuryltrione against Eleocharis kuroguwai. When rice and E. kuroguwai were applied at a range of doses of tefuryltrione, $GR_{90}$ values (the dose required to inhibit weed growth by 90%) of E. kuroguwai were $82.38-93.39g\;a.i.\;ha^{-1}$ in two independent experiments. The $GR_{10}$ values (the dose required to inhibit rice growth by 10%) of tefuryltrione for rice were $297.77-471.54g\;a.i.\;ha^{-1}$. As a result, the selectivity indices ($GR_{10}$ for $rice/GR_{90}$ for E. kuroguwai) of tefuryltrione were 3.19-5.72. Therefore, these results demonstrate that tefuryltrione has a relatively high selectivity between rice and E. kuroguwai with a high herbicidal activity against E. kuroguwai and a good rice safety.

• Korean Journal of Weed Science
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• v.9 no.1
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• pp.34-38
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• 1989

This study was conducted to investigate the tuberization time and the control method for Eleocharis kuroguwai Ohwi. The control methods were compared the shoot cutting and bentazon treatment for E. kuroguwai with untreatment. The initial tuberization time of E. kuroguwai, when E. kuroguwai was planted and without rice planted, was about August 18th and 10th, respectively. When the shoot of E. kuroguwai was cut, the regrowth vigour scarcely revived for the cutting on September 12. The least tuberization of E. kuroguwai, when E. kuroguwai was just planted and planted with rice plant, were on August 28 and August 3 to the top cutting of E. kuroguwai, respectively. Bentazon treatments from July 19 to August 3 hardly induced tuberization of E. kuroguwai, while as the delay of bentazon treatment time the tuberization of E. kurguwai was little by little increased.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.219-224
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• 2016

This study was conducted to investigate the ecology of weed occurrence and to establish an economical paddy field weed control system for direct-seeded rice on water. The main problem weed among annual and perennial weeds was Eleocharis kuroguwai. The control effect of E. kuroguwai was low by the application of azimsulfuron + carfentrazone-ethyl + pyriminobac-methyl at the early stages of rice in directly seeded paddy fields, but most annual weeds including Echinochloa crus-galli were controlled. Also, the additional application of pyrazosulfuron-ethyl + thiobencarb GR at 20 days after first treatment or of bentazone SL at 43 days after the first treatment effectively controlled E. kuroguwai. Tuber formation of E. kuroguwai was inhibited by the additional application of pyrazosulfuron-ethyl + thiobencarb by 84%, but the dormancy rate of old tubers of E. kuroguwai under the soil was higher than that of E. kuroguwai tubers following single application of azimsulfuron + carfentrazone-ethyl + pyriminobac-methyl at the early stages of rice. Tuber formation was inhibited by the additional application of bentazone by approximately 87%, and the old tuber dormancy rate was low in the bentazone treatment. High amounts of old dormant tubers of E. kuroguwai were found in the weedy plots.

• Korean Journal of Weed Science
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• v.30 no.2
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• pp.94-102
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• 2010

Field and pot experiments were conducted to investigate seedling emergence and early growth of Eleocharis kuroguwai panted on different dates. Non-linear regression analyses of observed data against effective accumulated temperature (EAT) with the Gompertz model showed that the Gompertz model works well in describing seedling emergence and early growth of E. kuroguwai regardless of planting date and soil burial depth. EATs required for 50% of the maximum seedling emergence of E. kuroguwai planted at 1, 3 and 5 cm soil burial depth in the pot experiment were estimated to be 54.5, 84.0 and $118.0^{\circ}C$, respectively, and $56.7^{\circ}C$ when planted at 1 cm in the field experiment. EATs required for 50% of the maximum leaf number of E. kuroguwai planted at 1, 3 and 5 cm soil burial depth in the pot experiment were estimated to be 213.3, 249.0 and $291.6^{\circ}C$, respectively, and $239.5^{\circ}C$ when planted at 1 cm in the field experiment. Therefore, models developed in this study thus predicted that if rotary tillage with water is made on 27 May under $+2^{\circ}C$ elevated temperature condition, dates for 50% of the maximum seedling emergence, 5 leaf stage and 5 cm plant height of E. kuroguwai buried at 3 cm soil depth were predicted to be 2 June, 10 June and 12 June. These dates are 1 day earlier for the seedling emergence and 3 days earlier for the early growth as compared with current temperature condition, suggesting that earlier application of herbicides is required for effective control of E. kuroguwai.

• Korean Journal of Weed Science
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• v.5 no.1
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• pp.43-49
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• 1985

This study was conducted to investigate the existance of dormancy in newly collected tubers of Eleocharis kuroguwai Ohwi from paddy fields and to determine the effect of various growth regulators on the bud sprouting and tuberization of E. kuroguwai. The maximum percent sprouting of E. kuroguwai collected at every month during winter was less than 60% regardless of varied collection tunes until 50 days after incubation, suggesting the presence of dormancy in E. kuroguwai tubers. This dormancy was markedly broken as the time went by, probably due to the exposure of tubers the extremly low temperature. The treatment of $BA10^{-3}M$ and $BA10^{-3}+GA10^{-6}M$ increased bud sprouting about 10 and 11 times, respectively, than that of the untreated control indicating that BA seems to be one of the most effective agents among the growth regulators used on the bud sprouting of E. kuroguwai. Foliar application of BA increased tuberization of E. kuroguwai by an average of 34.4% as compared with the untreated control. The highest increase, 39.8% was obtained with BA treatment at $10^{-5}M$.

• Korean Journal of Weed Science
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• v.15 no.4
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• pp.254-261
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• 1995

The nationwide weed survey was conducted in lowland rice fields over whole country of Korea in 1992 in order to determine a change of weed community and to identify a major dominant weed species and/or problem weed. Based on morphological characteristics of weeds, population ratio of broad leaf weed was 42.6%, grasses weed-9.0%, sedges-33.4% and others were 15.0%. Annual weed was 33.4% while perennial weed was 66.6% in terms of life cycle of weeds. Meanwhile, there was different weed occurrence as affected by planting method of the rice plant. In hand transplanted paddy fields predominant weed species was Sagittaria trifolia L., Monochoria vaginalis Presl., and Aneilema japonica Kunth. In machine transplanted rice fields of infant and young rice seedling Eleocharis kuroguwai Ohwi. and S. trifolia L. were more predominant. There was high occurrence of M. vaginalis, Echinochloa crus-galli L., and Leesia japonica Makino in water seeding while E. crus-galli and Cyperus serotinus Rottb. were predominant weed species in dry seeded rice. Monoculture of the rice plant would cause to high occurrence of E. kuroguwai, S. trifolia, M. vaginalis, E. crus-galli, and Sagittaria pygmaea Miq and there was higher population of S. trifolia, S. pygmaea, M. vaginalis, E crus-galli, and E. kuroguwai in double cropping system based on rice culture. In particular, there was high different weed occurrence under different transplanting times. E. kuroguwai, S. trifolia, S. pygmaea, M. vaginalis, and C. serotinus were higher population at the transplanting of 25 May and S. trifolia, E crus-galli, C. serotinus, and M. vaginalis at 10 June and S. pygmaea, E. kuroguwai, M. vaginalis, S. trifolia, and E. crusgalli at 25 June in Korea, respectively. Autumn tillage in terms of tillage time would cause more predominant weed species such as S. trifolia, E. kuroguwai, M. vaginalis, and S. pygmaea while spring tillage was higher population of E. kuroguwai, S. trifolia, E. crusgalli, M. vaginalis, and S. pygmaea. In plain area of paddy field there was higher occurrence of E. kuroguwai, S. trifolia, M. vaginalis, E. crus-galli, and S. pygmaea and in mid-mountainous area S. trifolia, E. kuroguwai, M. vaginalis, E. crus-galli, and Ludwigia prostrate Roxb. while in mountainous area S. trifolia, M. vaginalis, Potamogeton distinctus Ben., E. kuroguwai, and E. crus-galli were. In 1992 the most ten predominant weed species at the rice field of Korea based on summed dominant ratio(SDR) were E. kuroguwai > S. trifolia > E. crus-galli > M. vaginalis > S. pygmaea > C. serotinus > L. prostrate > P. distinctus > A. japonica > Scirpus juncoides Roxb.

• Korean Journal of Weed Science
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• v.11 no.1
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• pp.60-67
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• 1991

The experiment was conducted to evaluate weeding effect and to inhibit tuberization of Eleocharis kuroguwai authority by single or systematic applications of several herbicides. E. kuroguwai was controlled above 90 percentage by pretilachlor and butachlor+bensulfuron application at 5-10 DAT(Days after transplanting) followed by bentazon at 50 DAT and weeded 88 percentage by mefenacet+bensulfuron application at 15 DAT. Tuberization inhibition rate of E. kuroguwai was higher at the application of butachlor+bensulfuron at 5 DAT followed by bentazon at 50 DAT than any other applications. The weight variation of E. kuroguwai tubers formed at 15-20cm soil stratum was larger than that at 10-15cm. Tuber of E. kuroguwai was hardly formed at top soil stratum (0-5cm) and deep soil (15-20cm) by the systematic application of bentazon at 50 DAT. Grain yield of rice from systematic application with bentazon at 50 DAT and mefenacet+bensulfuron at 10-15 DAT was similar to that of hand weeded plot.

• Journal of Life Science
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• v.30 no.5
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• pp.476-482
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• 2020

In rice agriculture, Eleocharis kuroguwai Ohwi (olbanggae) is a target for herbicidal intervention as a problem weed although it has also long been used clinically as a traditional medicine for jaundice, fever, and blood flow. E. kuroguwai has been evaluated in many clinical trials, but its molecular biological advantages are still unknown. Here, we investigate the anti-inflammatory effects of E. kuroguwai 80% ethanol extracts by screening NO production in LPS-induced macrophage activation. To find the most effective fractions, we partitioned five sub-fractions using HP20 column chromatography, namely 20%, 40%, 60%, 80%, and 100%. Of these, the 60% and 80% sub-fractions were found to significantly inhibit NO production; there were no toxicological effects at any concentration. In addition, the 80% sub-fraction inhibited significantly the iNOS and the mRNA of the pro-inflammatory mediators IL-6, TNF-α, and IL-1β by inhibiting the phosphorylation of JNK and ERK pathways associated with MAPKs signaling. Our results suggest that the 80% E. kuroguwai sub-fraction has the most significant anti-inflammatory effects of inhibiting iNOS and pro-inflammatory mediators and suppressing the phosphorylation of JNK and ERK. Therefore, the 80% sub-fraction of E. kuroguwai extract may be a therapeutic candidate for inflammatory diseases associated with the overexpression of MAPKs.

• Korean Journal of Weed Science
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• v.13 no.1
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• pp.44-54
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• 1993

This experiment was conducted to understand the environmental factors affecting growth and tuber formation such as temperature, day length, tight intensity, water condition and cutting time of Eleocharis kuroguwai Ohwi. Plant height, shoot number and dry weight of E. kuroguwai were higher at high temperature, 25/$25^{\circ}C$ (day/night), while nitrogen content was higher at low temperature, 20/$15^{\circ}C$. Plant height was more affected by water temperature, while shoot number and dry weight were more affected by air temperature. Contents and absorption of nitrogen, phosphorus, and potassium in top parts of E. kuroguwai were higher under greater difference between air and water temperatures, i.e., 18/$28^{\circ}C$ and 28/$18^{\circ}C$. The number and weight of tubers were increased under greater difference between air and water temperatures, i.e, 18/$28^{\circ}C$ and 28/$18^{\circ}C$, while they were inhibited at low or high air/water temperatures (18/$18^{\circ}C$ or 28/$28^{\circ}C$). Tubers of E. kuroguwai were formed at 8-or 12-hour day length, however, no tuber was formed at l6-hour day length. Photoinductive period for tuber initiation of E. kuroguwai was between 30 and 45 days after emergence, and the induction period of short-day treatment was less than 10 days. Tuber number and weight were reduced by shading due to inhibition of the growth of top and underground parts. Number of days from planting to tuber initiation was shortned as planting time was delayed and plant height, dry weight, and tuber number were also reduced by delayed planting. Tuber number at l0 to 15cm water depth was decreased 63 to 75% as compared with 1 to 5cm water depth. Tuber number and dry weight were not affected by the size of tubers at planting. Due to the reduced growth of top and underground parts, tuber number and dry weight of E. kuroguwai were decreased by delayed shoot cutting. The critical cutting time to inhibit the growth of E. kuroguwai was about 70 days after emergence.

• The Korean Journal of Pesticide Science
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• v.6 no.1
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• pp.36-41
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• 2002

Characteristics of regrowth and starch degradation in perennial weed rhizomes were investigated in a greenhouse. Cyperus serotinus started regrowth at 24 days after treatment of 1.25 g ai/ha of pyrazosulfuron-ethyl. The regrowth of Sagittaria trifolia, Eleocharis kuroguwai, and S. pygmaea required $30{\sim}39$ days, and Potamogeton distinctus regrew at 55 days after treatment of 1.25 g ai/ha of pyrazosulfuron-ethyl. However, all of 5 perennial weeds hardly regrew until 45 days after treatment more than 5 g ai/ha of pyrazosulfuron-ethyl. Regrowth of C. serotinus 4-node rhizomes was 2 times higher than 2-node rhizomes, and $1{\sim}1.5g$ of E. kuroguwai large tubers regrew faster than $0.3{\sim}0.5g$ of small tubers treated with bensulfuron-methyl. Regrowth of C. serotinus was enhanced with delayed application of bensulfuron-methyl, however, 2-leaf stage of E. kuroguwai plants regrew better than 3 leaf stage of plants when treated with equal volume of bensulfuron-methyl. The critical periods of S. trifolia and E. kuroguwai growth depending upon the rhizome detachment were 12 and 18 days after sprouting, respectively. The starch stacked in the S. trifolia and E. kuroguwai tubers were exhausted at 18 and 27 days after sprouting, respectively. The highest level of sugar contents was showed at 9 days after sprouting in S. trifolia, and 18 days after sprouting in E. kuroguwai.