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http://dx.doi.org/10.5352/JLS.2020.30.5.476

Eleocharis kuroguwai Ohwi Ameliorates LPS-mediated Inflammation by Suppressing MAPKs Signaling  

Kim, Kang-Hoon (Immunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology)
Bae, Eun Young (Elohim Co., Ltd, R&D Center)
Lim, Hyun Goo (Department of Plant Resources, College of Industrial Science, Kongju National University)
Kim, Mi Heui (Department of Agricultural Environment Rural Environment and Resources Division, National Institute of Agricultural Sciences)
Oh, Tae-Seok (Department of Plant Resources, College of Industrial Science, Kongju National University)
Lee, Seung Woong (Immunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Life Science / v.30, no.5, 2020 , pp. 476-482 More about this Journal
Abstract
In rice agriculture, Eleocharis kuroguwai Ohwi (olbanggae) is a target for herbicidal intervention as a problem weed although it has also long been used clinically as a traditional medicine for jaundice, fever, and blood flow. E. kuroguwai has been evaluated in many clinical trials, but its molecular biological advantages are still unknown. Here, we investigate the anti-inflammatory effects of E. kuroguwai 80% ethanol extracts by screening NO production in LPS-induced macrophage activation. To find the most effective fractions, we partitioned five sub-fractions using HP20 column chromatography, namely 20%, 40%, 60%, 80%, and 100%. Of these, the 60% and 80% sub-fractions were found to significantly inhibit NO production; there were no toxicological effects at any concentration. In addition, the 80% sub-fraction inhibited significantly the iNOS and the mRNA of the pro-inflammatory mediators IL-6, TNF-α, and IL-1β by inhibiting the phosphorylation of JNK and ERK pathways associated with MAPKs signaling. Our results suggest that the 80% E. kuroguwai sub-fraction has the most significant anti-inflammatory effects of inhibiting iNOS and pro-inflammatory mediators and suppressing the phosphorylation of JNK and ERK. Therefore, the 80% sub-fraction of E. kuroguwai extract may be a therapeutic candidate for inflammatory diseases associated with the overexpression of MAPKs.
Keywords
Anti-inflammation; Eleocharis kuroguwai Ohwi; iNOS; MAPK;
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