• Title/Summary/Keyword: E. coil

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Biosynthetic Regulation of Invertase from Recombinant E. coil pYC17 (재조합 대장균 pYC17이 생성하는 세포내 invertase의 생합성 조절)

  • Yi, Sung-Hun;No, Jae-Duck;Lee, Dae-Hyung;Lee, Jong-Soo
    • The Journal of Natural Sciences
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    • v.17 no.1
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    • pp.103-111
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    • 2006
  • Regulation of invertase biosynthesis was studied with the E. coil harboring recombinant plasmid, pYC17. Biosynthesis of invertase in the recombinant E. coil was effectively induced in the presence of 30mM of sucrose for 3h. Glucose also repressed the invertase induction in the recombinant E. coil at 10 mM, lower than that of parent strain (30 mM).

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In Vivo High Resolution NMR Imaging by Using Surface Gradient Coil (평면 경사자계 코일을 사용한 고분해능 NMR 생체 영상법에 관한 연구)

  • Yi, Jeong-Han;Oh, Woo-Jin;Cho, Zang-Hee
    • Proceedings of the KOSOMBE Conference
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    • v.1990 no.11
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    • pp.48-51
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    • 1990
  • A new in vivo high resolution imaging method which is performed with a newly developed three channel surface gradient coil (SGC) is described. The surface gradient coil can produce more than an order of magnitude stronger gradient fields with good linearity within a limited imaging region. To increase the signal to noise ratio (SNR), we have developed an RF coil integrated surface gradient coil set. In this paper, the geometrical structures and characteristics of the proposed surface gradient coil are discussed and experimentally obtained high resolution images ($50\;{\mu}m$ to $100\;{\mu}m$) of a water filled phantom and a human volunteer's knee using the new surface RF coil integrated SGC set are presented for the demonstration of the in vivo high resolution imaging capability of the new imaging method.

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Test of the Model Coil for a SMES (SMES용 Model Coil의 특성시험)

  • Kim, H.J.;Seong, K.C.;Cho, J.W.;Kwon, Y.K.;Ryu, K.S.;Ryu, K.
    • Proceedings of the KIEE Conference
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    • 2001.07b
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    • pp.726-728
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    • 2001
  • For the development of a small-sized superconducting magnetic energy storage (SMES) system we designed, fabricated and tested the model coil consisting five coils with different features, e.g. winding tensions, bore diameters and materials, cooling channels. The results show that even in the highly pre-stressed small coil A, about 70 % of the coils critical current are degraded. The quench current of the coils A, B and E with narrow cooling channels is two times as high as that of the coil C without them though they are similar except spacers. The test results also indicate that the usual training effect depends on the winding tensions of the coils but the quench characteristic does not change according to materials of a bobbin.

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Fabrication and Test of the Model Coil for a $\mu$ SMES Magnet ($\mu$ SMES 마그네트용 Model Coil의 제작 및 특성시험)

  • 김해종;성기철;조전욱;이언용;권영길;류강식;류경우
    • Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
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    • 2001.02a
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    • pp.89-91
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    • 2001
  • For the development of a small-sized superconducting magnetic energy storage (SMES) system we designed, fabricated and tested the model coil consisting five coils with different features, e.g. winding tensions, bore diameters and materials, cooling channels. The results show that even in the highly pre-stressed small coil A, about 70 % of the coils critical current are degraded The quench current of the coils A, B and E with narrow cooling channels is two times as high as that of the coil C without them though they are similar except spacers. The test results also indicate that the usual training effect depends on the winding tensions of the coils but the quench characteristic does not change according to materials of a bobbin.

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Analysis on electrical and thermal characteristics of MI-SS racetrack coil under conduction cooling and external magnetic field

  • Chae, Yoon Seok;Kim, Ji Hyung;Quach, Huu Luong;Lee, Sung Hoon;Kim, Ho Min
    • Progress in Superconductivity and Cryogenics
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    • v.23 no.4
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    • pp.61-69
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    • 2021
  • This paper presents the analysis and experiment results on the electrical and thermal characteristics of metal insulation (MI) REBCO racetrack coil, which was wound with stainless steel (SS) tape between turn-to-turn layers, under rotating magnetic field and conduction cooling system. Although the field windings of superconducting rotating machine are designed to operate on a direct current, they may be subjected to external magnetic field due to the unsynchronized armature windings during electrical or mechanical load fluctuations. The field windings show the voltage and magnetic field fluctuations and the critical current reduction when they are exposed to an external magnetic field. Moreover, the cryogenic cooling conditions are also identified as the factors that affect the electrical and thermal characteristics of the HTS coil because the characteristic resistance changes according to the cryogenic cooling conditions. Therefore, it is necessary to investigate the effect of external magnetic field on the electrical and thermal characteristics of MI-SS racetrack coil for further development reliable HTS field windings of superconducting rotating machine. First, the major components of the experiment test (i.e., HTS racetrack coil construction, armature winding of 75 kW class induction motor, and conduction cooling system) were fabricated and assembled. Then, the MI racetrack coil was performed under liquid nitrogen bath and conduction cooling conditions to estimate the key parameters (i.e., critical current, time constant, and characteristic resistance) for the test coil in the steady state operation. Further, the test coil was charged to the target value under conduction cooling of 35 K then exposed to the rotating magnetic field, which was generated by three phrase armature windings of 75 kW class induction motor, to investigate the electrical and thermal characteristics during the transient state.

Design of Rogowski coil to improve of current measurement sensitivity (전류측정감도 개선을 위한 로고우스키 코일의 설계)

  • Park, J.N.;Lee, C.;Jang, Y.M.;Kang, S.H.;Lim, K.J.;Na, D.H.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.11b
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    • pp.609-612
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    • 2001
  • Rogowski coil is made having no ferromagnetic material in a core. So the coil cannot be driven into saturation. This result in that Rogowski coils may be calibrated at relatively low currents. and used with confidence at very high currents. However the lowest level of current that can be measured is limited by the sensitivity of the voltage measuring instrument and system noise. Therefore. geometrical effects were investigated in order to measure high sensitivity of low level current and the significant source of error was examined as well. In the results, the source of error was associated with coil designs. i.e. shape and uniformity of coil and a geometrical location of current source inside and outside of the Rogowski coil.

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Design of Rogowski coil to improve of current measurement sensitivity (전류측정감도 개선을 위한 로고우스키 코일의 설계)

  • Park, J. N.;Lee, C.;Jang, Y. M.;Kang, S. H.;Lim, K. J.;Na, D. H.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.11a
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    • pp.609-612
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    • 2001
  • Rogowski coil is made having no ferromagnetic material in a core. So the coil cannot be driven into saturation. This result in that Rogowski coils may be calibrated at relatively low currents, and used with confidence at very high currents. However the lowest level of current that can be measured is limited by the sensitivity of the voltage measuring instrument and system noise. Therefore, geometrical effects were investigated in order to measure high sensitivity of low level current and the significant source of error wa examined as well. n the results, the source of error was associated with coil designs, i.e. shape and uniformity of coil and a geometrical location of current source inside and outside of the Rogowski coil.

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Expression of Yeast Antioxidant Protein Gene in E. coil (대장균(JM 109)에서 효모 Thiol-Specific Antioxident 단백질의 발현)

  • Kim, Il-Han
    • The Journal of Natural Sciences
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    • v.4
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    • pp.1-10
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    • 1991
  • The expression of the cloned Saccharomyces cerevisiae antioxidant protein gene in E. coil on plasmid was studied. Introduction of the DNA encoding yeast thiol-specific antioxidant protein into expression vector, PKK 223-3, having the tac promoter resulted in the production in E. coil of a Mr 27,000 polypeptide. This protein represented as much as about 1% of the bacterial soluble protein and showed the thiol-specific antioxidant properties. The bacterial protein showed physico-chemical properties indistringuishable from those of the yeast protein.

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Dynamic Characteristics of Moving Coil Type Linear Oscillatory Actuator (가동코일형 리니어 왕복 액추에이터의 동특성)

  • Jang, S.M.;Jeong, S.S.;Kweon, C.;Park, H.C.;Moon, S.J.;Park, C.I.;Chung, T.Y.
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.49 no.3
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    • pp.180-187
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    • 2000
  • A moving-coil-type linear oscillatory actuator(LOA) consists of the NdFeB permanent magnets with high specific energy as the stator, a coil-wrapped nonmagnetic hollow rectangular structure. The LOA system was represented by the voltage equation of coil and the mechanical equation of motion. This set of equations was manipulated in state-space form. The EMF constant kE of equation parameters in state-space form can be obtained by using the induced voltage in armature coils at open circuit test. kE and other parameters provide the system matrices and transfer function for frequency response and dynamic simulation. Voltage source inverter-fed LOA is examined aiming to compare with results of simulation.

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Development of Micro-size Search Coil Magnetometer for Magnetic Field Distribution Measurement

  • Ka, E.M.;Son, De-Rac
    • Journal of Magnetics
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    • v.13 no.1
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    • pp.34-36
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    • 2008
  • For the measurement of the magnetic field distribution with high spatial resolution and high accuracy, the magnetic field sensing probe must be non-magnetic, but the MFM probe and sub-millimeter-meter size Hall probe use a ferromagnetic tip and block, respectively, to increase the sensitivity. To overcome this drawback, we developed a micro-size search coil magnetometer which consists of a single turn search coil, Terfenol-D actuator, scanning system, and control software. To reduce the noise generated by the stray ac magnetic field of the actuator driving coil, we employed an even function $\lambda$-H magnetostriction curve and lock-in technique. Using the developed magnetometer, we were able to measure the magnetic field distribution with a magnetic field resolution of 1 mT and spatial resolution of $0.1mm{\times}0.2mm$ at a coil vibration frequency of 1.8 kHz.